ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0.05 0.05 0.05 0 0 0.05 0.05 0.05 0.07 0 0.05 0.07 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0.07 0 0.06 0 0.05 0.06 0.05 0.06 0.07 0.14 0.21 0.16 0.09 0.17 0 0 0 0 0 0
Cytoplasm 0.18 0.22 0.22 0.23 0.22 0.23 0.16 0.22 0.24 0.2 0.23 0.15 0.12 0.1 0.1 0.44 0.5 0.43 0 0.06 0.06 0 0 0
Endoplasmic Reticulum 0.78 0.77 0.6 0.62 0.5 0.35 0.38 0.13 0.39 0.4 0.27 0.36 0.78 0.81 0.76 0.07 0.05 0 0.84 0.8 0.76 0.74 0.73 0.72
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.09 0.17 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.22 0 0.06 0.08 0 0.22 0.14 0 0 0 0.1 0.08 0.1 0.05 0 0.07 0.06 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0 0.26 0.23 0.35 0.3 0.51 0.61 0.32 0.42 0.33 0.37 0.11 0.08 0.08 0.35 0.36 0.42 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 7 5 9 18 11 9 9 14 14 14 26 8 26 39 5 3 3 4 8 1 9 24 23
Bud 0 0 1 1 1 6 4 15 7 4 7 7 1 2 3 0 1 0 0 2 0 2 9 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 6 4
Bud Site 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 6 5 8 25 8 25 9 15 18 13 23 24 74 126 20 11 14 0 0 3 11 16 17
Cytoplasm 28 42 22 44 79 52 69 102 66 56 63 60 39 53 60 56 65 35 4 11 7 21 24 27
Endoplasmic Reticulum 121 148 60 120 181 80 158 61 108 113 74 144 255 446 448 9 7 2 136 167 99 346 469 439
Endosome 1 0 1 2 3 1 3 7 2 1 2 2 0 0 0 1 3 1 1 1 0 2 2 9
Golgi 0 0 0 2 9 0 3 0 1 2 0 2 7 6 12 16 11 14 1 1 0 3 1 4
Mitochondria 3 4 0 4 6 50 17 28 23 10 61 57 2 9 1 13 10 8 8 4 9 29 33 29
Nucleus 0 3 2 0 2 3 5 11 5 2 5 6 1 1 2 4 2 1 0 1 0 1 0 0
Nuclear Periphery 3 2 0 0 1 2 3 0 4 2 3 11 9 5 3 0 0 0 0 1 0 12 11 4
Nucleolus 0 0 1 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0
Vac/Vac Membrane 17 7 26 44 127 69 216 285 87 118 91 147 35 44 49 45 46 34 1 4 4 20 31 36
Unique Cell Count 156 191 100 194 364 230 420 469 274 280 274 400 326 548 593 128 129 81 163 209 130 470 641 613
Labelled Cell Count 179 219 123 234 452 283 512 528 332 340 335 486 381 667 743 169 160 112 163 209 130 470 641 613


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 9.0 7.9 8.6 9.9 7.3 9.6 10.9 10.2 9.6 11.1 9.5 12.7 15.9 17.8 11.7 10.9 12.9 7.8 8.0 8.4
Std Deviation (1e-4) 1.4 1.8 1.8 1.6 1.9 2.8 1.8 13.3 11.8 1.8 14.0 1.9 3.1 3.9 4.1 3.1 2.7 3.3 1.9 2.3 2.4
Intensity Change (Log2) – – – 0.13 0.33 -0.1 0.29 0.47 0.38 0.28 0.49 0.28 0.7 1.01 1.18 0.58 0.48 0.72 -0.01 0.02 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0.6 0 0 0.6 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.7 0 0.4 0 0.2 0.5 0 0.3 0.8 2.4 3.8 2.5 1.0 2.7
Cytoplasm 0.1 -0.1 0.1 -1.3 -0.1 0.4 -0.4 0.2 -1.7 -2.5 -3.5 -3.4 3.4 4.4 3.1
Endoplasmic Reticulum 0.3 -1.8 -4.3 -4.1 -10.4 -3.5 -3.4 -5.9 -4.4 3.6 4.8 3.3 -8.6 -9.0 -8.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.7 0 4.3
Mitochondria 0 0 5.1 0 0 3.0 0 5.2 4.0 0 0 0 3.3 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 1.7 0.7 4.6 6.3 1.1 2.9 1.3 2.0 -3.8 -5.3 -5.3 1.5 1.6 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1187 6.9065 5.6286 5.7621 5.4991 5.9945 5.453 6.903 6.125 5.7358 5.1012 6.1772 8.3035 9.9845 8.6589 9.0243 8.0338 8.678
Actin 0.0139 0.0004 0.0243 0.0029 0.0009 0.0007 0.0161 0.0002 0.0127 0.0128 0.0231 0.0007 0.0086 0.0008 0.0015 0.0002 0.0003 0.001
Bud 0.0001 0 0.0007 0.0002 0 0.0003 0.0004 0 0.0001 0.0001 0.0008 0 0 0.0001 0 0 0 0.0001
Bud Neck 0.0007 0.0001 0.0025 0.0106 0.0001 0.0006 0.0019 0.0001 0.0004 0.0003 0.002 0.0004 0.002 0.0002 0.0002 0.0001 0.0001 0.0007
Bud Periphery 0.0001 0 0.0008 0.0002 0.0001 0.0006 0.0005 0 0.0002 0.0004 0.001 0.0001 0.0001 0.0001 0.0001 0 0 0.0004
Bud Site 0.0004 0.0002 0.0058 0.0032 0.0001 0.0004 0.0016 0.0001 0.001 0.0003 0.0035 0 0.0005 0.0018 0.0003 0 0 0
Cell Periphery 0.0003 0.0027 0.002 0.0006 0.0001 0.0005 0.0005 0.0011 0.0021 0.0013 0.0004 0.0002 0.0006 0.0027 0.0016 0.0004 0.0004 0.0005
Cytoplasm 0.0351 0.0405 0.0562 0.0907 0.0148 0.0314 0.0291 0.04 0.0388 0.0208 0.0543 0.0425 0.0292 0.1075 0.0537 0.0496 0.034 0.0372
Cytoplasmic Foci 0.0052 0.0016 0.0038 0.0045 0.0011 0.0026 0.0161 0.0014 0.012 0.0031 0.019 0.0014 0.0011 0.0014 0.0014 0.0024 0.0008 0.0007
Eisosomes 0.0001 0 0.0005 0 0 0 0.0002 0 0.0001 0.0005 0.0005 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.8477 0.9026 0.8545 0.818 0.8984 0.9306 0.7413 0.9255 0.8759 0.9083 0.6867 0.907 0.9203 0.8605 0.9042 0.931 0.9431 0.9445
Endosome 0.0193 0.0033 0.007 0.0137 0.0253 0.0029 0.0479 0.0055 0.0141 0.006 0.0585 0.0081 0.0052 0.0038 0.0049 0.0073 0.004 0.0023
Golgi 0.0048 0.0004 0.0021 0.0052 0.0016 0.0002 0.0177 0.0005 0.0055 0.0011 0.0342 0.0014 0.001 0.0008 0.0014 0.0004 0.0004 0.0003
Lipid Particles 0.0033 0.0139 0.0023 0.0291 0.0028 0.0015 0.0208 0.013 0.0099 0.0036 0.0341 0.008 0.0026 0.0044 0.0033 0.0022 0.0016 0.0016
Mitochondria 0.0013 0.0001 0.001 0.0003 0.0018 0.0001 0.0064 0.0001 0.0039 0.0011 0.0343 0.0042 0.0004 0.0003 0.0011 0.0001 0.0001 0.0001
None 0.0011 0.0012 0.0055 0.0003 0.0013 0.0028 0.0132 0.0004 0.0008 0.0158 0.0022 0.0004 0.0019 0.0017 0.0069 0.0008 0.0031 0.0007
Nuclear Periphery 0.0502 0.0218 0.0223 0.0123 0.0398 0.0188 0.0464 0.0078 0.0083 0.0101 0.0137 0.0165 0.0216 0.0065 0.0144 0.0034 0.009 0.0071
Nucleolus 0.0003 0 0.0003 0.0001 0.0004 0.0001 0.0012 0 0.0001 0.0002 0.0006 0.0001 0 0 0 0 0 0
Nucleus 0.0068 0.0017 0.0027 0.0029 0.0045 0.0026 0.0108 0.0005 0.0009 0.0019 0.0052 0.0018 0.0014 0.0011 0.0008 0.0003 0.0013 0.001
Peroxisomes 0.0011 0 0.0003 0.0005 0.0002 0 0.01 0 0.0081 0.0006 0.0108 0.0007 0.0002 0 0.0001 0.0001 0 0
Punctate Nuclear 0.0026 0.0005 0.0018 0.0012 0.0013 0.001 0.0077 0.0001 0.0011 0.0058 0.0016 0.002 0.0005 0.0003 0.0002 0.0002 0.0002 0.0002
Vacuole 0.0039 0.0084 0.003 0.0027 0.0033 0.0018 0.0074 0.0035 0.0032 0.0046 0.0109 0.0031 0.0021 0.0058 0.0027 0.0013 0.0014 0.0015
Vacuole Periphery 0.0016 0.0007 0.0006 0.0005 0.0019 0.0004 0.0029 0.0002 0.0007 0.0015 0.0026 0.0012 0.0005 0.0003 0.0011 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 73.5603 61.3745 94.3381 70.787 43.4482 102.9274 98.9936 103.7113 104.3689 75.3487
Translational Efficiency 1.1342 1.2686 1.0862 1.6391 1.3285 1.0739 1.1933 1.7684 1.5629 1.34

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1624 385 207 2211 629 2166 2474 1238 2253 2551 2681 3449

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1021.94 999.02 1319.87 978.89 966.22 939.54 1166.24 1124.40 1006.38 948.52 1178.10 1031.12
Standard Deviation 251.15 179.42 186.95 167.06 276.10 137.55 189.41 191.67 259.57 146.21 193.61 189.61
Intensity Change Log 2 — -0.032725 0.369085 -0.062092 — -0.040397 0.271441 0.218732 — -0.036448 0.322456 0.081214

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001079 0.001429 0.005222 0.004824 0.000941 0.000677 0.001913 0.004188 0.001040 0.000790 0.002168 0.004595
Bud Neck 0.001049 0.002802 0.003888 0.012212 0.001503 0.002955 0.002156 0.007036 0.001176 0.002932 0.002289 0.010354
Bud Site 0.010721 0.011632 0.010126 0.038026 0.021067 0.004886 0.008889 0.036351 0.013609 0.005905 0.008985 0.037425
Cell Periphery 0.005803 0.002502 0.003583 0.005074 0.006332 0.001784 0.003351 0.003937 0.005950 0.001893 0.003369 0.004666
Cytoplasm 0.016965 0.081608 0.049323 0.222319* 0.029357 0.136768* 0.061809 0.129140* 0.020425 0.128443* 0.060845 0.188873*
Cytoplasmic Foci 0.011773 0.033657 0.007693 0.017611 0.033586 0.017921 0.001978 0.022323 0.017863 0.020296 0.002419 0.019303
Eisosomes 0.000108 0.000296 0.000241 0.000941 0.000101 0.000672 0.000171 0.000473 0.000106 0.000615 0.000176 0.000773
Endoplasmic Reticulum 0.915291* 0.771182* 0.723284* 0.480298* 0.777307* 0.744911* 0.875072* 0.626269* 0.876768* 0.748876* 0.863352* 0.532694*
Endosome 0.006306 0.011064 0.016593 0.008129 0.021751 0.007071 0.003920 0.014119 0.010618 0.007674 0.004898 0.010279
Golgi 0.005393 0.008808 0.001111 0.003265 0.013131 0.009042 0.000839 0.008488 0.007553 0.009007 0.000860 0.005139
Lipid Particles 0.004903 0.018439 0.011914 0.018084 0.012636 0.019774 0.003463 0.016743 0.007062 0.019573 0.004116 0.017603
Mitochondria 0.004150 0.003885 0.000295 0.003753 0.003563 0.006496 0.001028 0.002815 0.003986 0.006102 0.000972 0.003417
Mitotic Spindle 0.000262 0.002912 0.001274 0.002322 0.000972 0.000574 0.000252 0.007250 0.000460 0.000927 0.000331 0.004091
None 0.003170 0.004243 0.000104 0.003129 0.013479 0.007462 0.003072 0.001025 0.006048 0.006976 0.002843 0.002374
Nuclear Periphery 0.000418 0.002542 0.031430 0.001836 0.007765 0.003468 0.000899 0.001291 0.002469 0.003329 0.003257 0.001640
Nuclear Periphery Foci 0.004085 0.009750 0.087747 0.012814 0.027059 0.004738 0.006673 0.015090 0.010498 0.005495 0.012933 0.013631
Nucleolus 0.000219 0.000128 0.000037 0.000270 0.001423 0.000185 0.000042 0.000230 0.000555 0.000176 0.000042 0.000256
Nucleus 0.002056 0.008026 0.007538 0.081283 0.007461 0.017723 0.011935 0.023299 0.003565 0.016260 0.011596 0.060470
Peroxisomes 0.001766 0.001726 0.000206 0.001268 0.001322 0.002550 0.000216 0.000983 0.001642 0.002425 0.000216 0.001166
Vacuole 0.003051 0.020404 0.037861 0.081437 0.011196 0.008426 0.012114 0.071853 0.005325 0.010234 0.014102 0.077997
Vacuole Periphery 0.001433 0.002965 0.000529 0.001105 0.008048 0.001917 0.000207 0.007098 0.003280 0.002075 0.000232 0.003256

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.80 -1.68 -7.96 -5.66 0.19 1.22 -2.19 -6.23 -6.89 -3.67 1.39 -2.55 -10.00 -10.90 -4.42
Bud Neck -1.98 -2.64 -12.12 -8.90 -5.42 -2.22 -1.49 -8.88 -5.61 -7.34 -2.78 -2.33 -14.29 -10.38 -12.36
Bud Site -0.36 -0.19 -9.68 -7.60 -6.57 4.80 3.38 -3.87 -10.48 -8.77 4.81 2.32 -10.37 -16.00 -13.18
Cell Periphery 2.52 0.86 0.95 -2.50 -0.42 3.21 2.06 1.63 -4.13 -0.99 4.86 2.93 1.70 -7.52 -2.64
Cytoplasm -8.71 -3.62 -41.90 -16.63 -15.50 -20.48 -8.27 -16.42 -0.35 -12.10 -27.92 -15.60 -43.45 -12.87 -29.86
Cytoplasmic Foci -4.80 0.93 -4.26 3.29 -3.59 3.99 8.42 2.34 -2.67 -11.61 -1.32 10.55 -1.64 -0.22 -18.11
Eisosomes -3.01 -1.35 -10.70 -6.74 -5.47 -3.96 -1.67 -5.74 1.34 -5.11 -4.22 -2.37 -12.10 -1.06 -10.52
Endoplasmic Reticulum 8.41 8.89 47.44* 17.38 10.52 2.07 -6.33 10.04 11.26 22.27 15.31 2.60 42.50 26.66 42.88*
Endosome -3.58 -4.86 -2.45 2.34 4.09 6.73 8.44 3.30 -5.84 -9.57 3.14 7.08 0.47 -3.27 -8.71
Golgi -2.19 5.80 2.58 3.85 -3.08 1.82 5.76 1.98 0.62 -4.87 -1.40 8.40 2.69 4.52 -5.99
Lipid Particles -4.74 -2.58 -10.36 0.15 -1.80 -2.90 4.04 -1.68 1.60 -9.54 -9.74 3.15 -9.21 1.45 -14.07
Mitochondria 0.19 5.03 0.69 0.26 -8.46 -3.07 3.19 0.84 4.77 -2.99 -2.80 5.16 1.07 4.89 -7.35
Mitotic Spindle -1.05 -2.50 -3.48 0.22 -1.06 1.03 2.12 -3.90 -4.15 -4.36 -1.03 0.50 -5.23 -3.97 -5.34
None -0.52 4.04 0.21 0.64 -6.43 1.53 2.72 3.27 5.87 3.17 -0.60 2.48 3.06 4.75 0.90
Nuclear Periphery -2.87 -5.41 -6.45 0.86 5.15 2.26 3.74 3.52 3.81 -2.70 -1.21 -1.09 1.47 3.28 3.17
Nuclear Periphery Foci -3.65 -8.64 -8.49 -1.99 7.69 5.59 5.02 2.73 -8.11 -6.20 3.93 -1.66 -2.47 -10.48 -0.72
Nucleolus 1.07 2.08 -0.78 -4.50 -9.36 2.78 3.10 2.60 -0.70 -2.44 2.72 3.71 1.99 -2.50 -6.84
Nucleus -3.42 -3.51 -28.24 -23.57 -21.85 -4.37 -2.17 -7.13 -4.29 -6.81 -10.16 -6.92 -28.40 -21.59 -23.24
Peroxisomes 0.07 5.23 1.67 1.35 -7.72 -2.37 2.90 0.81 4.07 -5.13 -2.07 6.14 1.94 4.00 -10.04
Vacuole -5.07 -5.74 -27.10 -16.16 -7.08 1.65 -1.22 -16.15 -17.62 -16.22 -4.90 -8.30 -31.78 -29.72 -26.86
Vacuole Periphery -1.88 3.59 1.53 2.36 -1.93 4.52 5.98 0.69 -3.27 -4.56 2.38 7.77 0.31 -1.62 -5.38
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
Localization
Cell Percentages ER (96%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

YDR476C

YDR476C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr476c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available