Standard name
Human Ortholog
Description MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG2 has a paralog, DIG1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.76 0.76 0.74 0.58 0.37 0.26 0.22 0.18 0.14 0.16 0.12 0.76 0.86 0.82 0.19 0.2 0.2 0.44 0.46 0.41 0.44 0.31 0.23
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.07 0 0 0 0 0 0
Endosome 0 0.06 0.09 0.11 0.13 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.05 0.08 0 0.07 0.58 0.59 0.48 0.56 0.6 0.64 0.53 0 0 0 0.12 0.07 0.05 0.05 0 0.11 0.07 0.05 0
Nucleus 0.09 0.05 0 0.05 0.07 0.12 0.18 0.3 0.3 0.29 0.31 0.37 0.09 0.07 0.06 0.67 0.56 0.68 0.19 0.14 0.07 0.09 0.17 0.25
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0.05
Vac/Vac Membrane 0.15 0.13 0.14 0.15 0.29 0.18 0.12 0.11 0.16 0.09 0.09 0.1 0.12 0.06 0.15 0.09 0.24 0.07 0.17 0.16 0.16 0.3 0.33 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 10 0 1 1
Bud 0 0 2 2 2 15 13 12 11 13 19 15 0 0 0 0 0 1 3 20 11 3 5 8
Bud Neck 0 0 2 0 0 0 1 4 0 2 3 0 0 1 0 0 0 0 1 8 3 1 5 2
Bud Site 0 0 0 1 2 7 16 12 20 18 20 18 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0
Cytoplasm 114 542 437 314 399 321 262 201 126 116 95 84 104 152 191 20 11 17 84 385 265 99 104 72
Endoplasmic Reticulum 0 2 1 0 1 1 2 0 0 0 1 1 2 1 1 8 1 6 0 1 12 2 4 5
Endosome 1 41 54 48 86 5 21 29 21 25 6 34 4 2 7 1 0 4 3 23 35 1 5 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 3 2 0 1 1
Mitochondria 10 39 44 15 51 499 604 438 393 497 389 371 2 1 2 12 4 4 9 33 72 16 15 13
Nucleus 13 35 14 22 49 102 188 275 208 241 186 258 12 12 14 70 30 59 36 114 45 21 57 80
Nuclear Periphery 0 1 0 0 1 2 2 3 1 4 2 2 0 0 0 0 0 0 0 4 6 0 1 1
Nucleolus 0 0 1 0 0 19 22 41 14 40 21 24 0 0 0 0 1 0 2 5 5 0 1 0
Peroxisomes 0 2 2 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 3 0 0 0
SpindlePole 0 3 4 8 11 6 7 15 23 19 9 19 0 0 0 0 0 2 10 61 40 7 11 16
Vac/Vac Membrane 22 93 80 63 201 160 123 102 110 76 52 73 16 11 35 9 13 6 32 136 101 68 109 101
Unique Cell Count 148 713 576 424 687 866 1017 920 702 832 609 701 136 177 234 104 54 87 191 841 650 229 334 322
Labelled Cell Count 160 759 641 473 803 1137 1261 1132 927 1053 803 899 140 181 251 120 60 99 191 841 650 229 334 322


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.2 3.9 4.1 4.2 3.3 3.6 4.0 3.8 3.7 3.7 3.8 4.9 5.1 5.2 5.6 5.7 5.6 4.6 5.0 5.2
Std Deviation (1e-4) 0.4 0.8 1.2 1.3 1.5 1.3 1.3 1.0 1.1 1.1 1.0 0.9 0.8 1.2 1.4 1.0 1.0 0.9 0.8 1.4 1.1
Intensity Change (Log2) – – – 0.07 0.12 -0.23 -0.13 0.03 -0.01 -0.06 -0.08 -0.02 0.32 0.38 0.41 0.52 0.56 0.53 0.24 0.37 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -1.5 2.0 -1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.797 -0.3241 -0.638 -0.6443 -1.3399 -0.7051 0.05 0.9545 0.8602 0.5102 0.4017 0.7321 -1.0993 -0.6097 -0.8973 -0.1061 -0.3368 -0.5222
Actin 0.0089 0.0007 0.0052 0.0002 0.0003 0.0007 0.0013 0.0002 0.0003 0.0008 0.0008 0.0002 0.0002 0.0008 0.0002 0.1354 0 0.0026
Bud 0.0003 0.0002 0.0002 0.0003 0.0001 0.0001 0.0005 0.0001 0.0006 0.0001 0.0003 0 0.0004 0.0003 0.0001 0.0007 0 0.0001
Bud Neck 0.006 0.001 0.0005 0.001 0.0008 0.0053 0.0016 0.0006 0.0004 0.0004 0.0017 0.0046 0.0006 0.0006 0.0002 0.0008 0.0001 0.0028
Bud Periphery 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0003 0 0.0006 0.0002 0 0.0008 0 0.0001
Bud Site 0.0045 0.0013 0.0016 0.0003 0.0002 0.0003 0.002 0.0009 0.0003 0.0001 0.0002 0.0001 0.001 0.0034 0.0003 0.0019 0 0.0002
Cell Periphery 0.0003 0.0001 0 0 0 0 0.0002 0 0 0 0 0 0.0002 0.0001 0 0.0001 0 0.0001
Cytoplasm 0.1038 0.1467 0.0883 0.0937 0.0663 0.0773 0.0995 0.0626 0.0465 0.0186 0.0622 0.0534 0.192 0.1739 0.1868 0.0674 0.2088 0.1498
Cytoplasmic Foci 0.011 0.0076 0.0052 0.0008 0.0155 0.0047 0.0077 0.0028 0.0025 0.0031 0.0034 0.0047 0.0033 0.0051 0.0052 0.0353 0.0035 0.0064
Eisosomes 0.0005 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0 0 0.0002 0 0.0001
Endoplasmic Reticulum 0.0046 0.0054 0.002 0.0008 0.0015 0.0013 0.0024 0.0016 0.0007 0.0002 0.0018 0.0007 0.0048 0.0032 0.0032 0.0011 0.0007 0.0041
Endosome 0.0119 0.012 0.0029 0.0006 0.0059 0.0044 0.0035 0.0045 0.0014 0.0006 0.003 0.0016 0.0075 0.0035 0.0028 0.0149 0.0026 0.0133
Golgi 0.0017 0.0003 0.0006 0 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0013 0.0001 0.0643 0 0.0181
Lipid Particles 0.0039 0.0008 0.0011 0 0.0015 0.0006 0.0004 0.0003 0.0002 0.0003 0.0004 0.0002 0.0002 0.0069 0.0003 0.0216 0 0.0063
Mitochondria 0.001 0.0008 0.0006 0.0006 0.0005 0.0004 0.0009 0.0008 0.0007 0.0003 0.0007 0.0004 0.0008 0.0008 0.0004 0.007 0.0001 0.0269
None 0.3145 0.2681 0.2417 0.0895 0.2719 0.2285 0.247 0.1208 0.2239 0.201 0.2065 0.1391 0.2787 0.3274 0.3038 0.1275 0.1167 0.1474
Nuclear Periphery 0.0317 0.0266 0.0183 0.0056 0.0156 0.0129 0.0118 0.0184 0.0078 0.0048 0.019 0.0071 0.0257 0.0074 0.0102 0.0055 0.0014 0.0086
Nucleolus 0.0062 0.0054 0.0054 0.0038 0.0073 0.0111 0.0091 0.0102 0.0082 0.006 0.0147 0.0137 0.003 0.0074 0.0029 0.0012 0.0017 0.004
Nucleus 0.4341 0.4635 0.573 0.7747 0.5075 0.5919 0.5321 0.7132 0.6523 0.7164 0.546 0.6954 0.4475 0.4289 0.4718 0.4512 0.6625 0.5905
Peroxisomes 0.0014 0.0002 0.001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0002 0.0004 0.0002 0.0001 0.0001 0.0019 0.0001 0.0518 0 0.004
Punctate Nuclear 0.0488 0.0538 0.051 0.0271 0.1023 0.059 0.0764 0.0587 0.0531 0.0463 0.1359 0.0778 0.0101 0.0254 0.0099 0.0105 0.0009 0.0118
Vacuole 0.0036 0.0045 0.001 0.0005 0.002 0.0008 0.0022 0.0031 0.0006 0.0002 0.0021 0.0006 0.0217 0.0015 0.0014 0.0004 0.0008 0.002
Vacuole Periphery 0.001 0.0009 0.0003 0.0002 0.0004 0.0003 0.0004 0.0006 0.0002 0.0001 0.0007 0.0001 0.0013 0.0003 0.0002 0.0003 0.0002 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.9752 22.3254 12.8013 16.5729 19.8696 32.9023 23.8557 18.0054 18.0744 22.3304
Translational Efficiency 1.8236 1.9947 0.9884 1.0426 1.783 2.5612 1.2171 1.2538 1.082 1.5206

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1893 1673 2184 1460 1476 2562 1650 1365 3369 4235 3834 2825

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 637.91 726.68 743.20 776.07 660.44 713.05 759.58 746.33 647.78 718.43 750.25 761.70
Standard Deviation 72.89 88.75 88.71 136.69 71.24 103.19 91.80 104.23 73.03 97.97 90.42 122.99
Intensity Change Log 2 — 0.187967 0.220398 0.282834 — 0.110576 0.201774 0.176386 — 0.149119 0.210954 0.229668

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000241 0.000302 0.000173 0.001897 0.000057 0.000362 0.000212 0.000423 0.000161 0.000338 0.000190 0.001185
Bud Neck 0.008977 0.017336 0.009182 0.028153 0.006590 0.040781 0.006586 0.009857 0.007931 0.031520 0.008065 0.019312
Bud Site 0.003037 0.005641 0.010772 0.053380 0.001856 0.019172 0.004971 0.041017 0.002519 0.013827 0.008275 0.047406
Cell Periphery 0.000170 0.000135 0.000180 0.000667 0.000084 0.000236 0.000094 0.000332 0.000133 0.000196 0.000143 0.000505
Cytoplasm 0.466137* 0.358266* 0.187142* 0.260475* 0.400437* 0.376952* 0.136583* 0.386825* 0.437353* 0.369570* 0.165384* 0.321526*
Cytoplasmic Foci 0.120160 0.132140 0.005093 0.008781 0.087723 0.131553 0.001529 0.023041 0.105949 0.131785 0.003559 0.015671
Eisosomes 0.000083 0.000087 0.000026 0.000037 0.000051 0.000088 0.000020 0.000051 0.000069 0.000088 0.000024 0.000044
Endoplasmic Reticulum 0.000610 0.001801 0.001687 0.004296 0.000683 0.001529 0.002588 0.001670 0.000642 0.001636 0.002075 0.003027
Endosome 0.005444 0.008562 0.001182 0.004153 0.002898 0.013199 0.000606 0.003104 0.004329 0.011367 0.000934 0.003646
Golgi 0.000618 0.001783 0.000078 0.002412 0.000444 0.002471 0.000045 0.000602 0.000542 0.002200 0.000063 0.001537
Lipid Particles 0.007254 0.010289 0.000861 0.000971 0.005785 0.007792 0.000614 0.001669 0.006610 0.008778 0.000755 0.001308
Mitochondria 0.002783 0.009479 0.001635 0.003729 0.003610 0.017467 0.001163 0.002366 0.003145 0.014311 0.001432 0.003070
Mitotic Spindle 0.001265 0.008600 0.005525 0.033591 0.001828 0.023759 0.009715 0.021755 0.001512 0.017771 0.007328 0.027872
None 0.005444 0.006424 0.009579 0.012791 0.005501 0.005819 0.006883 0.011752 0.005469 0.006058 0.008418 0.012289
Nuclear Periphery 0.000976 0.002167 0.001741 0.002725 0.001344 0.002057 0.002193 0.001832 0.001137 0.002101 0.001936 0.002294
Nuclear Periphery Foci 0.000270 0.000910 0.000240 0.000831 0.000391 0.000658 0.000359 0.000468 0.000323 0.000757 0.000291 0.000655
Nucleolus 0.001936 0.003686 0.001995 0.001202 0.002382 0.004033 0.001077 0.001621 0.002131 0.003896 0.001600 0.001404
Nucleus 0.346436* 0.390604* 0.750933* 0.533342* 0.463994* 0.287500* 0.814475* 0.444773* 0.397940* 0.328230* 0.778279* 0.490547*
Peroxisomes 0.002233 0.010540 0.000253 0.001161 0.002330 0.011694 0.000151 0.000975 0.002276 0.011238 0.000209 0.001071
Vacuole 0.025368 0.030252 0.011332 0.043240 0.011727 0.050379 0.010007 0.044657 0.019392 0.042428 0.010762 0.043925
Vacuole Periphery 0.000556 0.000995 0.000391 0.002167 0.000284 0.002500 0.000129 0.001211 0.000437 0.001905 0.000278 0.001705

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.40 0.10 -2.29 -2.29 -2.50 -7.97 -9.61 -7.30 -1.08 -1.90 -1.84 -1.10 -2.96 -2.38 -2.73
Bud Neck -4.79 -0.55 -6.94 -2.16 -6.56 -17.29 -1.69 -3.69 14.54 -2.08 -16.84 -1.41 -7.68 9.24 -6.58
Bud Site -2.51 -4.68 -9.67 -8.77 -6.90 -12.23 -3.13 -8.68 -3.17 -7.62 -11.54 -5.59 -12.90 -7.28 -10.10
Cell Periphery 0.77 0.15 -3.90 -5.68 -5.06 -8.20 -0.90 -6.34 -0.13 -5.92 -2.57 -0.01 -5.53 -4.48 -6.92
Cytoplasm 12.63 39.15 33.24 18.62 -3.68 2.50 34.38 7.01 5.35 -24.05 11.10 51.53 26.79 17.05 -19.24
Cytoplasmic Foci -2.26 31.28 30.58 28.73 -1.51 -8.07 23.61 18.00 30.67 -11.88 -6.61 38.99 34.50 42.88 -10.42
Eisosomes -1.32 15.24 8.23 9.50 -9.93 -8.59 7.70 -1.50 8.02 -11.04 -5.89 16.81 5.70 12.44 -14.91
Endoplasmic Reticulum -9.56 -13.69 -18.23 -13.69 -12.60 -5.17 -14.67 -13.48 -7.28 4.19 -9.47 -19.64 -22.04 -15.33 -8.07
Endosome -3.57 9.52 5.46 7.44 -3.68 -12.10 6.12 0.78 13.00 -6.58 -11.20 11.27 5.03 14.91 -6.75
Golgi -3.31 5.40 -0.90 1.69 -2.54 -5.52 4.16 0.47 5.68 -3.24 -6.32 6.81 -0.64 4.82 -3.31
Lipid Particles -3.94 12.32 11.72 14.59 -3.22 -3.01 10.45 8.19 14.04 -7.76 -4.37 16.11 14.20 20.49 -7.87
Mitochondria -5.60 1.90 -0.28 6.03 -3.53 -10.47 3.27 2.20 13.45 -2.07 -12.25 3.58 1.42 14.48 -3.88
Mitotic Spindle -4.65 -4.16 -8.55 -5.40 -6.76 -9.28 -3.92 -6.99 0.67 -3.63 -10.48 -5.63 -11.05 -2.55 -7.53
None -1.32 -4.18 -4.97 -3.43 -0.67 -0.37 -0.72 -2.37 -2.27 -2.08 -1.07 -3.76 -4.66 -3.94 -1.86
Nuclear Periphery -5.61 -10.54 -18.34 -8.94 -12.00 -4.11 -7.76 -9.78 -4.54 0.46 -7.20 -12.02 -20.28 -9.96 -7.44
Nuclear Periphery Foci -7.33 -1.39 -7.79 -0.62 -7.00 -1.68 -2.19 -2.82 -0.31 -0.12 -4.43 -2.52 -7.45 -0.83 -4.55
Nucleolus -4.02 0.36 3.69 6.96 4.75 -3.39 3.36 2.88 7.83 -0.59 -5.67 2.65 4.45 10.95 2.66
Nucleus -4.78 -51.36 -25.88 -19.66 17.00 18.88 -38.16 -2.22 -19.50 31.95 10.61 -62.64 -19.53 -29.38 33.61
Peroxisomes -7.42 8.41 6.08 9.10 -2.24 -11.47 8.99 5.91 14.19 -3.17 -13.56 12.21 8.43 16.63 -3.80
Vacuole -2.90 5.04 -13.80 -12.33 -16.44 -18.65 -4.76 -16.71 -6.61 -13.55 -14.74 1.05 -21.08 -12.57 -21.42
Vacuole Periphery -4.22 2.41 -1.35 -0.00 -2.07 -7.69 3.46 -1.74 4.28 -2.25 -8.10 3.36 -2.09 3.69 -2.97
Standard name
Human Ortholog
Description MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG2 has a paralog, DIG1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (28%), cytoplasm (4%), mixed (35%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dig2

Dig2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dig2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available