Standard name | |
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Human Ortholog | |
Description | Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 1 | 6 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 2 | 4 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 4 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 76 | 15 | 103 | 90 | 44 | 91 | 123 | 184 | – | 162 | 197 | 225 | 589 | 753 | 765 | – | 37 | 31 | 12 | 3 | 6 | 18 | 10 | 10 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | – | 1 | 0 | 1 | 2 | 3 | 3 | – | 3 | 7 | 0 | 0 | 0 | 3 | 2 | 3 |
Endosome | 7 | 0 | 13 | 2 | 13 | 1 | 0 | 2 | – | 0 | 0 | 0 | 9 | 1 | 2 | – | 3 | 5 | 3 | 0 | 4 | 4 | 14 | 22 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 2 | 0 | 2 | 0 | 1 | 4 |
Mitochondria | 18 | 11 | 28 | 13 | 20 | 31 | 40 | 46 | – | 86 | 49 | 50 | 2 | 0 | 0 | – | 20 | 6 | 6 | 1 | 4 | 6 | 10 | 15 |
Nucleus | 31 | 7 | 29 | 28 | 59 | 67 | 129 | 125 | – | 77 | 30 | 46 | 22 | 12 | 7 | – | 102 | 72 | 1 | 0 | 1 | 0 | 2 | 2 |
Nuclear Periphery | 0 | 2 | 5 | 0 | 1 | 1 | 9 | 7 | – | 4 | 1 | 8 | 1 | 0 | 0 | – | 1 | 1 | 1 | 0 | 2 | 1 | 1 | 1 |
Nucleolus | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | – | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 2 | 0 | 0 | 1 | 2 | 5 |
Vac/Vac Membrane | 128 | 29 | 221 | 154 | 311 | 266 | 338 | 450 | – | 276 | 209 | 233 | 206 | 87 | 24 | – | 56 | 73 | 209 | 86 | 302 | 269 | 357 | 348 |
Unique Cell Count | 216 | 50 | 315 | 203 | 369 | 365 | 483 | 630 | 460 | 384 | 414 | 760 | 826 | 787 | 192 | 163 | 245 | 96 | 334 | 314 | 411 | 422 | ||
Labelled Cell Count | 264 | 64 | 402 | 289 | 451 | 459 | 639 | 815 | 607 | 493 | 565 | 834 | 857 | 809 | 223 | 199 | 245 | 96 | 334 | 314 | 411 | 422 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 4.4 | 5.0 | 5.8 | 7.7 | 6.6 | 6.2 | 6.2 | – | 5.1 | 5.4 | 5.5 | 5.6 | 5.4 | 5.2 | 4.9 | 5.3 | 6.5 | 6.2 | 6.1 | 6.5 |
Std Deviation (1e-4) | 0.9 | 0.8 | 1.4 | 2.0 | 2.0 | 2.3 | 2.3 | 2.1 | – | 1.8 | 1.8 | 1.6 | 0.9 | 0.9 | 0.9 | 1.3 | 1.5 | 2.0 | 1.3 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.21 | 0.63 | 0.4 | 0.31 | 0.3 | – | 0.04 | 0.1 | 0.13 | 0.16 | 0.12 | 0.06 | -0.03 | 0.09 | 0.37 | 0.32 | 0.3 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | -9.0 | -5.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.113 | 2.1166 | 1.8398 | 1.225 | 2.0136 | 1.9421 | 3.4329 | 3.752 | 3.4857 | 2.6484 | 3.113 | 3.4554 | 1.9023 | 3.4841 | 2.8183 | 2.6883 | 2.0574 | 3.1342 |
Actin | 0.0018 | 0.0001 | 0.0001 | 0.0002 | 0.0172 | 0.0003 | 0.0433 | 0 | 0.0202 | 0 | 0.0043 | 0.0042 | 0.021 | 0 | 0.0014 | 0.0003 | 0 | 0.0002 |
Bud | 0.0007 | 0.0035 | 0.0004 | 0.0013 | 0.0009 | 0.0011 | 0.0008 | 0.0004 | 0.0008 | 0.0003 | 0.0005 | 0.0004 | 0.0006 | 0.0005 | 0.001 | 0.0002 | 0.0003 | 0.0005 |
Bud Neck | 0.0055 | 0.002 | 0.0007 | 0.0018 | 0.0028 | 0.0134 | 0.0009 | 0.0021 | 0.001 | 0.0006 | 0.0015 | 0.0013 | 0.0007 | 0.001 | 0.0019 | 0.0007 | 0.0005 | 0.0011 |
Bud Periphery | 0.0013 | 0.0027 | 0.0011 | 0.0013 | 0.0014 | 0.0022 | 0.0014 | 0.0012 | 0.0017 | 0.0005 | 0.001 | 0.0009 | 0.001 | 0.0012 | 0.003 | 0.0004 | 0.001 | 0.0013 |
Bud Site | 0.0014 | 0.0055 | 0.0012 | 0.0032 | 0.0033 | 0.0013 | 0.0019 | 0.0026 | 0.0048 | 0.0005 | 0.0026 | 0.0011 | 0.0021 | 0.0029 | 0.0066 | 0.0005 | 0.0005 | 0.0007 |
Cell Periphery | 0.0011 | 0.002 | 0.0009 | 0.0015 | 0.0015 | 0.0019 | 0.0013 | 0.0033 | 0.0026 | 0.0005 | 0.0019 | 0.0022 | 0.0029 | 0.0041 | 0.0029 | 0.0011 | 0.0023 | 0.0051 |
Cytoplasm | 0.0281 | 0.0471 | 0.0279 | 0.0175 | 0.0191 | 0.0228 | 0.0311 | 0.0222 | 0.0159 | 0.0706 | 0.002 | 0.0071 | 0.0216 | 0.0424 | 0.0152 | 0.0014 | 0.0013 | 0.0138 |
Cytoplasmic Foci | 0.0349 | 0.0222 | 0.0246 | 0.0166 | 0.0222 | 0.0263 | 0.0298 | 0.0311 | 0.0154 | 0.0143 | 0.0121 | 0.0081 | 0.0259 | 0.0101 | 0.0276 | 0.0221 | 0.0057 | 0.0232 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0071 | 0.0057 | 0.0037 | 0.002 | 0.0067 | 0.0044 | 0.0225 | 0.0094 | 0.0111 | 0.0052 | 0.0006 | 0.0042 | 0.0034 | 0.0045 | 0.0035 | 0.0004 | 0.0022 | 0.0028 |
Endosome | 0.3263 | 0.1795 | 0.2749 | 0.1988 | 0.1773 | 0.2375 | 0.2811 | 0.2097 | 0.2533 | 0.1985 | 0.2254 | 0.1843 | 0.1911 | 0.1644 | 0.2212 | 0.1874 | 0.2455 | 0.1642 |
Golgi | 0.0043 | 0.0028 | 0.004 | 0.003 | 0.0069 | 0.0034 | 0.0344 | 0.0048 | 0.0107 | 0.0052 | 0.0424 | 0.0055 | 0.0098 | 0.004 | 0.0285 | 0.029 | 0.0088 | 0.0064 |
Lipid Particles | 0.0159 | 0.0076 | 0.0089 | 0.0083 | 0.0106 | 0.0127 | 0.0489 | 0.0253 | 0.0153 | 0.1227 | 0.024 | 0.0094 | 0.0842 | 0.0084 | 0.0387 | 0.0158 | 0.0675 | 0.0585 |
Mitochondria | 0.0156 | 0.009 | 0.015 | 0.0073 | 0.0041 | 0.006 | 0.0137 | 0.0073 | 0.0292 | 0.002 | 0.1203 | 0.0067 | 0.0255 | 0.0195 | 0.0898 | 0.105 | 0.0279 | 0.0305 |
None | 0.0672 | 0.0705 | 0.0833 | 0.0057 | 0.0036 | 0.0155 | 0.0039 | 0.0133 | 0.0647 | 0.0624 | 0.0002 | 0.0106 | 0.0637 | 0.0117 | 0.0161 | 0.0002 | 0.0005 | 0.0227 |
Nuclear Periphery | 0.0127 | 0.0101 | 0.0066 | 0.0059 | 0.0163 | 0.0092 | 0.0241 | 0.0086 | 0.0254 | 0.0038 | 0.0007 | 0.0054 | 0.0046 | 0.0142 | 0.0039 | 0.0009 | 0.006 | 0.0114 |
Nucleolus | 0.0056 | 0.0091 | 0.0032 | 0.006 | 0.0097 | 0.0095 | 0.0012 | 0.0036 | 0.0051 | 0.0012 | 0.004 | 0.0019 | 0.0092 | 0.0059 | 0.0037 | 0.0028 | 0.0031 | 0.0027 |
Nucleus | 0.0123 | 0.0181 | 0.0048 | 0.0218 | 0.0217 | 0.0277 | 0.0048 | 0.0036 | 0.0058 | 0.0032 | 0.0027 | 0.0031 | 0.0032 | 0.0101 | 0.0091 | 0.0025 | 0.0026 | 0.0069 |
Peroxisomes | 0.0012 | 0.0008 | 0.0013 | 0.0009 | 0.0012 | 0.0008 | 0.0109 | 0.0018 | 0.0151 | 0.0046 | 0.005 | 0.0014 | 0.0123 | 0.0005 | 0.0042 | 0.0194 | 0.0017 | 0.0033 |
Punctate Nuclear | 0.0023 | 0.0074 | 0.0009 | 0.023 | 0.0022 | 0.0018 | 0.0012 | 0.0004 | 0.0008 | 0.0004 | 0.0002 | 0.0006 | 0.0035 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0004 |
Vacuole | 0.3886 | 0.528 | 0.4576 | 0.5952 | 0.5945 | 0.517 | 0.3396 | 0.5498 | 0.3767 | 0.4223 | 0.5169 | 0.6049 | 0.3911 | 0.5534 | 0.3954 | 0.5125 | 0.4741 | 0.5144 |
Vacuole Periphery | 0.0662 | 0.0663 | 0.0788 | 0.0787 | 0.0768 | 0.0851 | 0.1029 | 0.0993 | 0.1243 | 0.0812 | 0.0314 | 0.1366 | 0.1222 | 0.1407 | 0.1259 | 0.0972 | 0.1485 | 0.1299 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 103.1401 | 66.1063 | 75.7321 | 144.3841 | 162.7416 | 116.0494 | 92.1982 | 96.7545 | 150.8955 | 228.8561 |
Translational Efficiency | 1.0064 | 1.0706 | 0.9898 | 0.8215 | 0.8908 | 0.9191 | 0.8174 | 0.8385 | 0.827 | 0.8039 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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