Standard name
Human Ortholog
Description Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0 0.08 0.05 – 0 0 0 0 0 0.19 0.16 0.22 0.13 0.2 0.21 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.31 0.17 0 0.3 – 0.26 0.36 0.34 0.4 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.87 0.87 0.88 0.89 0.79 0.75 – 0.74 0.73 0.73 0.78 0.74 0.78 0.71 0.69 0.49 0.53 0.5 0.87 0.88 0.84 0.68 0.66 0.63
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0.15 0.1 0.12 0.12 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.13 0 0 0.12 0.08 – 0 0 0 0 0 0.06 0.1 0.09 0.42 0.27 0.4 0 0 0.06 0.11 0.15 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 2 0 2 – 6 3 1 8 2 0 0 0 0 0 0 7 6 4 2 8 6
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2
Bud Site 0 0 0 0 2 10 – 22 4 15 27 19 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 16 7 9 12 29 21 – 28 16 21 13 22 27 27 48 18 23 27 1 1 4 3 5 3
Endoplasmic Reticulum 2 1 1 0 0 0 – 0 0 0 0 0 0 0 1 5 3 3 0 0 1 0 0 0
Endosome 0 3 0 0 9 3 – 4 1 3 0 0 0 3 2 1 3 0 3 3 1 2 5 3
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 1 1 1 1 0 0 0 2 2
Mitochondria 17 1 82 49 14 122 – 169 134 181 199 183 0 2 1 0 5 2 2 3 1 0 1 4
Nucleus 278 263 231 258 287 302 – 480 274 387 390 377 112 119 152 68 60 64 288 286 231 49 129 100
Nuclear Periphery 0 0 1 1 1 8 – 13 3 7 7 6 0 1 1 0 0 0 0 0 0 0 1 1
Nucleolus 2 1 2 3 3 12 – 96 38 65 60 68 1 4 1 1 1 0 4 7 4 0 6 5
Peroxisomes 0 0 0 0 0 1 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 0 0 0 – 1 0 0 0 0 1 0 0 0 0 0 2 4 5 1 3 0
Vac/Vac Membrane 34 40 10 9 42 31 – 23 14 17 15 11 8 17 20 59 30 51 14 9 16 7 28 23
Unique Cell Count 318 301 263 289 363 404 652 374 529 501 508 144 167 221 139 113 128 332 326 277 73 196 159
Labelled Cell Count 349 317 337 334 387 512 842 487 697 719 688 149 175 226 154 127 148 332 326 277 73 196 159


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 8.4 4.8 5.9 6.7 5.3 – 5.5 5.2 5.3 5.3 5.4 7.7 7.8 7.6 10.7 10.1 10.4 6.8 8.4 8.1
Std Deviation (1e-4) 1.6 1.8 1.1 1.5 2.1 1.5 – 1.5 1.6 1.4 1.7 1.4 2.3 2.4 2.3 2.3 2.4 2.1 2.1 2.6 2.1
Intensity Change (Log2) – – – 0.29 0.46 0.14 – 0.2 0.12 0.14 0.13 0.16 0.67 0.69 0.65 1.14 1.05 1.11 0.49 0.8 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 3.0 0 0 3.8 3.2 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 2.4 1.1 – 0.6 0.5 0.4 -0.6 0.6 5.2 4.7 6.2 3.6 5.4 5.7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.9 -9.4 -0.3 – -1.6 1.2 0.9 2.3 1.3 -7.5 -7.6 -8.9 -7.4 -5.6 -6.7
Nucleus 0.5 -2.9 -4.1 – -4.7 -4.5 -4.7 -3.4 -4.4 -2.7 -4.3 -5.1 -8.5 -7.4 -8.2
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.9 – 6.2 4.8 5.5 5.4 5.8 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.4 3.5 2.0 – -0.2 0 -0.4 -0.6 -1.3 0.8 2.7 2.4 9.8 6.6 9.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1308 1.5708 1.3789 0.52 0.9181 1.3026 1.6127 1.8224 1.5516 1.4915 1.4721 1.4447 3.3435 3.6641 3.2717 3.1687 2.6033 2.9956
Actin 0.0245 0 0.0057 0 0.0076 0.001 0.0082 0.0006 0.0043 0.0559 0.03 0.0002 0.0172 0 0.0019 0.0032 0.0059 0.0022
Bud 0.0006 0 0.0001 0 0.0006 0.0003 0.0003 0 0.0006 0.0003 0.0024 0.0006 0.0004 0 0.0002 0.0005 0.0002 0.0001
Bud Neck 0.0024 0.0001 0.0003 0.0001 0.0024 0.0016 0.0019 0.0002 0.0007 0.0006 0.009 0.0012 0.0051 0.0002 0.0023 0.0015 0.001 0.0015
Bud Periphery 0.0009 0 0.0001 0 0.0009 0.0007 0.0008 0 0.0016 0.0005 0.0061 0.0002 0.0006 0 0.0003 0.0006 0.0003 0.0002
Bud Site 0.0056 0.0001 0.0006 0.0001 0.0026 0.0007 0.0026 0.0001 0.0036 0.0006 0.0084 0 0.0017 0.0001 0.0021 0.0015 0.0008 0.0002
Cell Periphery 0.0004 0 0.0001 0 0.0002 0.0001 0.0002 0 0.0005 0.0001 0.0012 0 0.0003 0 0.0001 0.0002 0 0
Cytoplasm 0.018 0.0013 0.0031 0.0007 0.0177 0.0047 0.0037 0.0029 0.0044 0.0086 0.002 0.0045 0.0125 0.008 0.0085 0.0132 0.0026 0.0011
Cytoplasmic Foci 0.0246 0 0.0047 0.002 0.0204 0.0005 0.0097 0.0011 0.0015 0.0057 0.0121 0.0005 0.0076 0 0.0015 0.0071 0.006 0.0003
Eisosomes 0.0006 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0005 0 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0025 0 0.0004 0.0002 0.0011 0.0007 0.0011 0.0004 0.0007 0.0018 0.003 0.0001 0.0033 0.0001 0.0012 0.0057 0.0005 0.0006
Endosome 0.0157 0 0.0114 0.037 0.031 0.0028 0.0144 0.0007 0.005 0.0265 0.0557 0.0002 0.0185 0.0001 0.0031 0.0109 0.0084 0.0022
Golgi 0.008 0 0.0043 0.0024 0.017 0.0006 0.003 0.0016 0.0045 0.0111 0.0297 0 0.006 0 0.0003 0.002 0.006 0.0008
Lipid Particles 0.0109 0 0.0095 0.0066 0.0039 0.0006 0.0086 0.0006 0.0009 0.0278 0.0496 0.0001 0.0111 0 0.0004 0.0017 0.0215 0.001
Mitochondria 0.0112 0.0001 0.0082 0.0025 0.0638 0.0092 0.0162 0.0007 0.0087 0.0103 0.0327 0.0002 0.0029 0.0001 0.001 0.0014 0.0101 0.0023
None 0.0252 0 0.0004 0.0002 0.0005 0.0053 0.0063 0.0003 0.004 0.0014 0.0023 0.0004 0.0018 0.0001 0.003 0.0072 0.0005 0.0003
Nuclear Periphery 0.0077 0.0033 0.0089 0.0022 0.003 0.0091 0.0173 0.0065 0.0113 0.0065 0.012 0.0055 0.0165 0.0024 0.0165 0.006 0.0086 0.0028
Nucleolus 0.0044 0.0032 0.0032 0.0025 0.0081 0.0076 0.0168 0.0039 0.0056 0.0021 0.0142 0.0093 0.0098 0.0033 0.0052 0.0017 0.0052 0.0089
Nucleus 0.8 0.9909 0.9278 0.9358 0.8079 0.9479 0.8489 0.9728 0.9239 0.8033 0.6875 0.9688 0.867 0.9836 0.9394 0.9086 0.8763 0.9699
Peroxisomes 0.0199 0 0.005 0.0004 0.0025 0.0002 0.0028 0.0054 0.0007 0.0063 0.0138 0 0.0036 0 0.0004 0.0007 0.0414 0.0003
Punctate Nuclear 0.0065 0.0007 0.0028 0.0014 0.0044 0.0044 0.0247 0.0018 0.0115 0.0265 0.0135 0.0081 0.0112 0.0019 0.0092 0.0241 0.0038 0.0045
Vacuole 0.0084 0.0001 0.0025 0.0041 0.0028 0.0013 0.0098 0.0004 0.0046 0.0017 0.0097 0.0002 0.0016 0.0001 0.0023 0.0018 0.0005 0.0004
Vacuole Periphery 0.0021 0 0.0009 0.0017 0.0014 0.0008 0.0027 0.0001 0.0012 0.002 0.0048 0.0001 0.0009 0 0.0012 0.0003 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.931 14.5055 12.5577 15.1855 17.532 8.1088 15.2776 11.5487 23.2522 16.391
Translational Efficiency 0.989 1.084 1.5522 1.725 1.3213 2.2707 0.8067 2.5271 1.1629 1.2442

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p
Localization
Cell Percentages nucleus (92%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cwc21

Cwc21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cwc21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available