Standard name | |
---|---|
Human Ortholog | |
Description | Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; involved in localization of actin and chitin; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 25 | 5 | 2 | 1 | 1 | 0 | 5 | – | 0 | 0 | 0 | 27 | 19 | 17 | 2 | 5 | 0 | 8 | 11 | 9 | 5 | 6 | 11 |
Bud | 5 | 16 | 11 | 5 | 6 | 14 | 19 | 32 | – | 47 | 23 | 17 | 8 | 9 | 11 | 7 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 2 | 0 | 1 | 0 | 3 | 5 | 13 | – | 11 | 8 | 10 | 0 | 1 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | – | 0 | 1 | 2 | 2 | 3 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 9 | 10 | 13 | 10 | 9 | 8 | 16 | – | 7 | 5 | 8 | 11 | 14 | 25 | 39 | 49 | 23 | 0 | 1 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 5 | 8 | 2 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 5 | 8 | 2 | 2 | 7 | 6 | 0 | 0 | 0 | 1 | 1 | 1 |
Endosome | 100 | 56 | 80 | 28 | 28 | 2 | 5 | 3 | – | 1 | 3 | 2 | 192 | 261 | 337 | 99 | 90 | 43 | 137 | 131 | 96 | 77 | 67 | 62 |
Golgi | 53 | 52 | 13 | 3 | 3 | 1 | 0 | 0 | – | 0 | 1 | 0 | 207 | 336 | 244 | 22 | 40 | 24 | 142 | 182 | 102 | 100 | 117 | 161 |
Mitochondria | 252 | 341 | 178 | 139 | 112 | 268 | 307 | 364 | – | 387 | 218 | 294 | 117 | 133 | 73 | 26 | 26 | 6 | 22 | 38 | 17 | 16 | 10 | 18 |
Nucleus | 0 | 0 | 1 | 2 | 0 | 3 | 4 | 1 | – | 5 | 0 | 1 | 0 | 4 | 4 | 1 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 2 | 0 | 2 | 4 | 4 | 2 | – | 5 | 2 | 4 | 0 | 1 | 2 | 1 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 11 | 6 | 5 | 1 | 3 | 7 | 14 | 8 | – | 25 | 7 | 25 | 4 | 11 | 18 | 16 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 21 | 37 | 72 | 29 | 16 | 15 | 26 | 44 | – | 27 | 16 | 16 | 64 | 102 | 103 | 81 | 64 | 20 | 111 | 76 | 118 | 150 | 168 | 151 |
SpindlePole | 46 | 22 | 70 | 16 | 20 | 11 | 27 | 44 | – | 33 | 13 | 22 | 95 | 147 | 181 | 77 | 59 | 20 | 15 | 4 | 10 | 10 | 2 | 0 |
Vac/Vac Membrane | 12 | 12 | 1 | 0 | 2 | 3 | 3 | 6 | – | 1 | 0 | 3 | 25 | 40 | 83 | 7 | 8 | 3 | 3 | 10 | 1 | 1 | 1 | 3 |
Unique Cell Count | 383 | 426 | 318 | 186 | 162 | 287 | 334 | 408 | 420 | 233 | 320 | 577 | 859 | 866 | 271 | 285 | 116 | 446 | 465 | 362 | 367 | 380 | 421 | |
Labelled Cell Count | 521 | 587 | 450 | 240 | 205 | 341 | 423 | 540 | 549 | 297 | 404 | 758 | 1090 | 1107 | 384 | 377 | 148 | 446 | 465 | 362 | 367 | 380 | 421 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.7 | 6.5 | 6.0 | 5.4 | 5.2 | 4.2 | 4.1 | 4.2 | – | 3.7 | 3.9 | 3.9 | 7.7 | 7.8 | 8.0 | 6.4 | 7.3 | 8.0 | 7.6 | 7.8 | 8.3 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.1 | 1.4 | 1.8 | 1.0 | 1.2 | 1.0 | – | 0.9 | 0.8 | 0.9 | 0.8 | 0.8 | 0.9 | 1.2 | 1.3 | 1.5 | 1.4 | 1.1 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.19 | -0.5 | -0.54 | -0.49 | – | -0.68 | -0.6 | -0.62 | 0.37 | 0.39 | 0.43 | 0.11 | 0.29 | 0.43 | 0.36 | 0.38 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.4 | 0.7 | 0.4 | 0 | 0 | 0 |
Bud | -0.5 | 0.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | -2.1 | -2.8 | -2.5 | -0.6 | -0.5 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.0 | 1.6 | 0 | -0.6 | 0.6 | – | -1.3 | -0.7 | -0.5 | -1.2 | -1.6 | -0.2 | 4.9 | 5.8 | 5.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.7 | -2.0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.5 | 1.8 | 4.4 | 3.0 | 1.7 | 2.4 |
Golgi | -1.5 | -1.3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 10.6 | 11.7 | 8.9 | 2.1 | 4.3 | 5.5 |
Mitochondria | 4.2 | 2.8 | 0 | 0 | 0 | – | 0 | 0 | 0 | -10.9 | -14.0 | -17.7 | -11.8 | -12.1 | -9.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.6 | 2.8 | 2.2 | 0 |
Peroxisomes | -1.9 | -3.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | -4.6 | -4.6 | -4.6 | 2.0 | -0.1 | -1.2 |
SpindlePole | -3.9 | -2.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | -2.0 | -1.9 | -0.4 | 1.8 | -0.4 | -1.1 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.4 | 3.6 | 5.5 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8629 | 1.5767 | 1.2069 | 1.3996 | 1.7169 | 1.4453 | 1.3634 | 1.9582 | 1.5264 | 1.313 | 1.0629 | 1.7268 | 0.8527 | 1.3005 | 1.2073 | 0.9018 | 0.9344 | 1.1359 |
Actin | 0.0041 | 0.0134 | 0.0039 | 0.001 | 0.0131 | 0.0074 | 0.0119 | 0.0021 | 0.0122 | 0.0007 | 0.0004 | 0.0037 | 0.0307 | 0.0257 | 0.0155 | 0.0019 | 0.0081 | 0.042 |
Bud | 0.0002 | 0.0011 | 0.0028 | 0.0001 | 0.0008 | 0.0001 | 0.0026 | 0.0002 | 0.0008 | 0.0001 | 0 | 0.0017 | 0.0003 | 0.0013 | 0.0004 | 0.0001 | 0.0001 | 0.0009 |
Bud Neck | 0.0008 | 0.0009 | 0.0005 | 0.0003 | 0.0015 | 0.0008 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0013 | 0.0005 | 0.0005 | 0.0004 | 0.0004 | 0.0011 |
Bud Periphery | 0.0002 | 0.0009 | 0.0009 | 0.0001 | 0.0019 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0005 | 0.0004 | 0.0012 | 0.0003 | 0 | 0.0001 | 0.0007 |
Bud Site | 0.0014 | 0.0156 | 0.0019 | 0.0003 | 0.0005 | 0.0003 | 0.0033 | 0.0007 | 0.0009 | 0.0004 | 0.0001 | 0.0003 | 0.0028 | 0.0041 | 0.0012 | 0.0005 | 0.0004 | 0.0011 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0045 | 0.0083 | 0.0046 | 0.0011 | 0.0021 | 0.0007 | 0.0022 | 0.002 | 0.0015 | 0.0021 | 0.0005 | 0.0009 | 0.0037 | 0.0029 | 0.0016 | 0.0006 | 0.0006 | 0.0011 |
Cytoplasmic Foci | 0.1689 | 0.2416 | 0.1596 | 0.1433 | 0.1135 | 0.1322 | 0.1463 | 0.1896 | 0.1495 | 0.1319 | 0.1416 | 0.1261 | 0.1558 | 0.2182 | 0.1871 | 0.1646 | 0.2103 | 0.1571 |
Eisosomes | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0006 | 0.0004 | 0.0003 | 0.0002 | 0.0014 | 0.0003 | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0034 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0013 |
Endosome | 0.2289 | 0.2275 | 0.2034 | 0.2393 | 0.1861 | 0.1105 | 0.1392 | 0.1624 | 0.1321 | 0.1149 | 0.1572 | 0.1048 | 0.1612 | 0.2039 | 0.1888 | 0.1708 | 0.2008 | 0.1011 |
Golgi | 0.1468 | 0.1796 | 0.1967 | 0.2064 | 0.2191 | 0.154 | 0.1492 | 0.1822 | 0.1795 | 0.192 | 0.1676 | 0.1813 | 0.1627 | 0.238 | 0.294 | 0.2752 | 0.2133 | 0.2423 |
Lipid Particles | 0.1408 | 0.0612 | 0.0975 | 0.0875 | 0.1493 | 0.1626 | 0.0901 | 0.0712 | 0.0588 | 0.1225 | 0.1049 | 0.0919 | 0.1965 | 0.0608 | 0.0503 | 0.1027 | 0.0881 | 0.1346 |
Mitochondria | 0.0319 | 0.0309 | 0.0492 | 0.0679 | 0.0254 | 0.0168 | 0.0204 | 0.0241 | 0.0308 | 0.0375 | 0.0156 | 0.0318 | 0.0267 | 0.0155 | 0.0276 | 0.0075 | 0.003 | 0.0291 |
None | 0.0029 | 0.0054 | 0.0013 | 0.0001 | 0.0012 | 0.0002 | 0.0032 | 0.0081 | 0.0011 | 0.0285 | 0.0001 | 0.0006 | 0.001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0008 |
Nuclear Periphery | 0.0008 | 0.0003 | 0.0002 | 0.0001 | 0.0188 | 0.0003 | 0.0033 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0005 | 0.0121 | 0.0001 | 0.0001 | 0 | 0 | 0.0041 |
Nucleolus | 0.0003 | 0.0021 | 0.0006 | 0.0001 | 0.0022 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0018 | 0 | 0.0005 |
Nucleus | 0.0003 | 0.0006 | 0.0004 | 0 | 0.0219 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0034 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0006 |
Peroxisomes | 0.2493 | 0.2025 | 0.2721 | 0.2499 | 0.2327 | 0.4107 | 0.4191 | 0.3549 | 0.4277 | 0.3664 | 0.4102 | 0.4518 | 0.2322 | 0.2234 | 0.2297 | 0.2709 | 0.2736 | 0.2768 |
Punctate Nuclear | 0.0032 | 0.0034 | 0.0013 | 0.0004 | 0.002 | 0.0011 | 0.0027 | 0.0006 | 0.0025 | 0.0005 | 0.0003 | 0.0022 | 0.0018 | 0.0012 | 0.0005 | 0.0016 | 0.0003 | 0.0025 |
Vacuole | 0.0126 | 0.0031 | 0.0017 | 0.0011 | 0.0034 | 0.0007 | 0.0011 | 0.0007 | 0.0006 | 0.0008 | 0.0005 | 0.0006 | 0.0016 | 0.0014 | 0.0011 | 0.0007 | 0.0004 | 0.001 |
Vacuole Periphery | 0.0014 | 0.001 | 0.001 | 0.0009 | 0.0029 | 0.0007 | 0.0008 | 0.0005 | 0.0005 | 0.0006 | 0.0005 | 0.0006 | 0.0015 | 0.0007 | 0.0008 | 0.0006 | 0.0004 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.5883 | 3.0728 | 5.3204 | 11.0403 | 4.4567 | 5.7258 | 9.3208 | 12.9313 | 9.3953 | 11.0563 |
Translational Efficiency | 0.9623 | 1.9516 | 1.3892 | 0.4872 | 1.2899 | 1.3775 | 0.9524 | 0.553 | 0.8555 | 0.9989 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
130 | 1178 | 1193 | 1192 | 1626 | 2647 | 673 | 1225 | 1756 | 3825 | 1866 | 2417 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 977.48 | 809.04 | 882.11 | 892.30 | 687.40 | 846.47 | 862.85 | 939.08 | 708.88 | 834.94 | 875.16 | 916.01 |
Standard Deviation | 151.69 | 113.79 | 118.68 | 136.06 | 86.31 | 103.23 | 117.64 | 114.76 | 119.88 | 107.99 | 118.67 | 127.87 |
Intensity Change Log 2 | — | -0.272856 | -0.148109 | -0.131538 | — | 0.300309 | 0.327960 | 0.450098 | — | -0.008142 | 0.067776 | 0.137513 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.015043 | 0.003165 | 0.008863 | 0.035292 | 0.001961 | 0.001893 | 0.004963 | 0.006149 | 0.002929 | 0.002285 | 0.007457 | 0.020522 |
Bud Neck | 0.003925 | 0.000490 | 0.001122 | 0.001218 | 0.001584 | 0.000220 | 0.002125 | 0.000835 | 0.001758 | 0.000303 | 0.001483 | 0.001023 |
Bud Site | 0.024205 | 0.001142 | 0.005403 | 0.012271 | 0.001457 | 0.000830 | 0.004564 | 0.004793 | 0.003141 | 0.000926 | 0.005100 | 0.008481 |
Cell Periphery | 0.000119 | 0.000050 | 0.000056 | 0.000076 | 0.000066 | 0.000019 | 0.000047 | 0.000030 | 0.000070 | 0.000029 | 0.000053 | 0.000053 |
Cytoplasm | 0.006773 | 0.000867 | 0.001564 | 0.004325 | 0.001219 | 0.000675 | 0.003767 | 0.002926 | 0.001630 | 0.000734 | 0.002359 | 0.003616 |
Cytoplasmic Foci | 0.312212* | 0.329316* | 0.440328* | 0.330044* | 0.207768* | 0.279610* | 0.340672* | 0.356490* | 0.215500* | 0.294918* | 0.404385* | 0.343447* |
Eisosomes | 0.000065 | 0.000049 | 0.000049 | 0.000075 | 0.000085 | 0.000035 | 0.000041 | 0.000031 | 0.000083 | 0.000040 | 0.000046 | 0.000053 |
Endoplasmic Reticulum | 0.000371 | 0.000171 | 0.000573 | 0.000311 | 0.000138 | 0.000038 | 0.000182 | 0.000162 | 0.000156 | 0.000079 | 0.000432 | 0.000235 |
Endosome | 0.223979* | 0.183702 | 0.205686* | 0.225079* | 0.183499 | 0.244024* | 0.310756* | 0.291059* | 0.186496 | 0.225447* | 0.243581* | 0.258519* |
Golgi | 0.168657* | 0.315598* | 0.206533* | 0.193419* | 0.418607* | 0.363248* | 0.188381* | 0.170801* | 0.400103* | 0.348573* | 0.199986* | 0.181956* |
Lipid Particles | 0.009961 | 0.035220 | 0.030498 | 0.010838 | 0.030900 | 0.012068 | 0.022727 | 0.009967 | 0.029350 | 0.019198 | 0.027695 | 0.010397 |
Mitochondria | 0.002870 | 0.015121 | 0.007413 | 0.008409 | 0.042022 | 0.025213 | 0.006675 | 0.008168 | 0.039124 | 0.022105 | 0.007147 | 0.008287 |
Mitotic Spindle | 0.057518 | 0.010410 | 0.035974 | 0.041266 | 0.004117 | 0.003389 | 0.035952 | 0.052878 | 0.008070 | 0.005551 | 0.035966 | 0.047151 |
None | 0.000034 | 0.000203 | 0.000021 | 0.000212 | 0.000491 | 0.000018 | 0.000068 | 0.000016 | 0.000457 | 0.000075 | 0.000038 | 0.000113 |
Nuclear Periphery | 0.000125 | 0.000032 | 0.000147 | 0.000057 | 0.000018 | 0.000013 | 0.000093 | 0.000066 | 0.000026 | 0.000019 | 0.000127 | 0.000062 |
Nuclear Periphery Foci | 0.000529 | 0.000504 | 0.002604 | 0.000758 | 0.000641 | 0.000230 | 0.000730 | 0.000633 | 0.000632 | 0.000314 | 0.001928 | 0.000695 |
Nucleolus | 0.000621 | 0.000541 | 0.000151 | 0.000072 | 0.001110 | 0.000076 | 0.000126 | 0.000146 | 0.001074 | 0.000219 | 0.000142 | 0.000109 |
Nucleus | 0.000308 | 0.000190 | 0.000116 | 0.000062 | 0.000397 | 0.000017 | 0.000274 | 0.000052 | 0.000391 | 0.000070 | 0.000173 | 0.000057 |
Peroxisomes | 0.146750* | 0.099704 | 0.046449 | 0.126025* | 0.101858* | 0.066467 | 0.067003 | 0.086053 | 0.105181* | 0.076703 | 0.053862 | 0.105766* |
Vacuole | 0.021759 | 0.001902 | 0.005242 | 0.008535 | 0.001175 | 0.001102 | 0.009112 | 0.007366 | 0.002699 | 0.001349 | 0.006638 | 0.007943 |
Vacuole Periphery | 0.004175 | 0.001621 | 0.001208 | 0.001657 | 0.000888 | 0.000815 | 0.001741 | 0.001378 | 0.001131 | 0.001063 | 0.001400 | 0.001516 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.78 | 0.91 | -2.68 | -9.20 | -7.09 | 0.16 | -2.68 | -3.41 | -3.39 | -0.73 | 1.07 | -4.13 | -9.15 | -9.79 | -6.30 |
Bud Neck | 2.89 | 2.43 | 2.33 | -4.73 | -0.61 | 3.28 | -0.59 | 1.69 | -5.15 | 1.48 | 3.78 | 0.61 | 1.82 | -7.55 | 1.20 |
Bud Site | 3.98 | 3.31 | 2.19 | -8.51 | -4.43 | 1.92 | -3.81 | -4.73 | -5.74 | -0.01 | 4.20 | -2.04 | -5.67 | -10.18 | -3.48 |
Cell Periphery | 1.95 | 1.80 | 1.24 | -2.94 | -2.50 | 8.47 | 1.62 | 6.13 | -4.05 | 1.97 | 6.90 | 2.41 | 2.54 | -5.73 | 0.02 |
Cytoplasm | 3.21 | 2.83 | 1.25 | -7.25 | -5.30 | 2.39 | -2.45 | -3.16 | -4.14 | 0.81 | 3.79 | -1.64 | -4.95 | -7.86 | -2.25 |
Cytoplasmic Foci | -1.06 | -7.02 | -1.06 | -0.02 | 12.88 | -15.29 | -16.11 | -21.56 | -11.08 | -1.56 | -17.90 | -31.38 | -23.46 | -9.36 | 9.48 |
Eisosomes | 1.46 | 1.43 | -0.65 | -6.16 | -5.70 | 4.90 | 4.04 | 5.34 | 1.53 | 2.54 | 4.70 | 3.89 | 3.26 | -5.09 | -2.01 |
Endoplasmic Reticulum | 1.99 | -1.21 | 0.58 | -4.02 | 1.96 | 4.64 | -1.01 | -0.35 | -2.58 | 0.54 | 3.59 | -3.19 | -2.16 | -5.39 | 2.26 |
Endosome | 2.00 | 0.83 | -0.16 | -5.56 | -2.47 | -10.40 | -12.52 | -13.52 | -6.07 | 1.67 | -7.43 | -8.82 | -11.66 | -6.05 | -2.31 |
Golgi | -8.34 | -2.30 | -1.50 | 15.69 | 1.97 | 7.61 | 25.89 | 32.80 | 30.45 | 2.07 | 7.58 | 28.77 | 32.21 | 34.04 | 3.50 |
Lipid Particles | -5.21 | -4.58 | -0.26 | 6.82 | 6.48 | 6.63 | 2.00 | 7.00 | 1.12 | 3.93 | 3.67 | 0.51 | 7.15 | 5.40 | 7.68 |
Mitochondria | -6.88 | -2.78 | -4.24 | 3.50 | -0.56 | 5.27 | 11.87 | 11.43 | 8.03 | -0.82 | 6.05 | 11.85 | 11.74 | 9.06 | -0.87 |
Mitotic Spindle | 3.83 | 1.67 | 1.20 | -8.16 | -1.18 | 1.09 | -6.95 | -11.45 | -11.68 | -2.80 | 2.05 | -9.32 | -13.32 | -14.91 | -3.02 |
None | -1.19 | 0.88 | -0.93 | -0.10 | -1.01 | 1.41 | 1.22 | 1.41 | -0.09 | 1.57 | 1.22 | 1.34 | 1.03 | -0.42 | -0.78 |
Nuclear Periphery | 1.51 | -0.14 | 1.14 | -2.19 | 2.94 | 1.92 | -4.48 | -4.87 | -5.44 | 1.38 | 1.26 | -4.94 | -4.48 | -6.52 | 3.14 |
Nuclear Periphery Foci | -0.73 | -2.76 | -1.06 | 0.04 | 2.25 | 1.43 | -0.92 | 0.01 | -2.23 | 0.97 | 0.80 | -2.49 | -0.28 | -1.49 | 2.52 |
Nucleolus | 0.13 | 0.92 | 1.02 | 1.23 | 0.64 | 2.57 | 1.90 | 1.19 | -1.02 | -0.44 | 2.18 | 2.38 | 1.95 | -0.20 | -0.44 |
Nucleus | 0.77 | 1.34 | 1.59 | 1.03 | 0.87 | 2.62 | 0.39 | 2.27 | -2.23 | 2.30 | 2.31 | 1.43 | 2.40 | 0.07 | 2.40 |
Peroxisomes | 2.34 | 5.09 | 1.03 | -3.21 | -11.17 | 6.93 | 5.14 | 2.51 | -3.58 | -2.67 | 5.80 | 9.99 | -0.05 | -6.23 | -10.63 |
Vacuole | 4.18 | 3.54 | 2.84 | -8.06 | -3.65 | 0.67 | -6.02 | -8.08 | -8.18 | 1.22 | 3.45 | -5.08 | -7.65 | -12.08 | -1.71 |
Vacuole Periphery | 1.14 | 1.32 | 1.13 | -0.07 | -1.13 | 0.73 | -2.92 | -2.50 | -2.86 | 1.06 | 0.37 | -1.09 | -1.29 | -1.81 | -0.48 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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