Standard name
Human Ortholog
Description Protein involved in late endosome to vacuole transport; cytoplasmic and vacuolar membrane protein; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.08 0.1 0.22 0.21 0.27 0.27 0.12 0.13 0.15 0 0.08 0.05 0.07 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0.06 0 0.06 0 0.06 0.06 0 0.12 0 0.05 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0.11 0 – 0.16 0.19 0.1 0.16 0.23 0.05 0.1 0 0.06 0.1 0.26 0.32 0.14 0.12 0.15 0.29 0.29 0.32 0.26 0.3 0.36
Golgi 0.07 0 – 0.24 0.28 0.17 0.09 0.08 0 0 0.08 0 0 0.11 0 0 0 0 0 0.05 0 0 0 0.06
Mitochondria 0.27 0.46 – 0.33 0.08 0.23 0.29 0.18 0.59 0.61 0.55 0.72 0.23 0.11 0.07 0.06 0 0 0.08 0.07 0.1 0.05 0 0.06
Nucleus 0 0.12 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.08 – 0 0 0 0 0 0 0 0 0 0.18 0.08 0 0 0.05 0 0 0 0.18 0.09 0 0.05
Nucleolus 0.21 0.08 – 0 0 0 0 0 0 0 0 0 0.18 0.09 0 0.09 0.1 0.13 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.65 0.5 – 0.47 0.46 0.41 0.34 0.28 0.16 0.13 0.23 0.08 0.56 0.53 0.68 0.76 0.82 0.73 0.54 0.46 0.28 0.48 0.55 0.38
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 1 5 0 1 2 3 4 0 6 0 1 0 0 0 0 0 0 0 0 0 1
Bud 0 0 – 0 1 0 1 1 1 3 1 3 0 1 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 1 1 1 1 1 2 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 0 1 – 4 13 18 18 31 27 8 8 18 1 7 4 11 8 9 0 1 0 0 0 4
Endoplasmic Reticulum 0 0 – 2 8 2 5 2 6 4 2 14 0 4 1 8 3 7 0 1 0 3 1 3
Endosome 8 1 – 8 24 8 14 27 5 7 2 7 4 22 27 22 21 20 30 19 18 18 46 51
Golgi 5 0 – 12 36 14 8 9 3 2 5 5 1 9 2 6 4 1 1 3 1 2 2 9
Mitochondria 19 11 – 16 10 19 25 21 59 41 33 85 9 9 6 9 6 3 7 4 5 3 7 7
Nucleus 0 3 – 0 1 1 1 1 3 1 3 1 0 2 0 5 6 6 0 0 0 1 2 0
Nuclear Periphery 3 2 – 0 0 0 1 0 1 0 0 0 7 7 2 4 8 5 3 1 10 6 6 6
Nucleolus 15 2 – 0 1 0 0 1 0 2 1 2 7 8 1 14 17 18 1 0 0 0 1 0
Peroxisomes 0 0 – 0 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 – 1 1 0 2 2 0 1 1 2 0 2 5 2 4 5 0 0 1 0 0 0
Vac/Vac Membrane 46 12 – 23 58 33 29 33 16 9 14 10 22 45 58 119 141 98 54 31 16 34 86 54
Unique Cell Count 71 24 49 127 81 85 116 100 67 60 118 39 85 85 156 173 135 102 69 58 73 157 143
Labelled Cell Count 97 32 67 158 96 106 132 125 83 72 153 51 118 107 200 218 173 102 69 58 73 157 143


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 7.2 – 10.3 10.6 10.1 8.3 8.7 7.6 7.3 8.0 7.3 8.1 9.5 9.6 9.0 10.8 10.8 11.2 11.6 12.7
Std Deviation (1e-4) 2.4 1.9 – 2.4 2.2 3.0 1.8 1.8 2.1 1.9 3.0 2.1 2.2 2.8 2.8 3.0 3.5 3.0 2.8 3.1 3.9
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3746 5.0875 4.2687 3.7045 3.2092 4.1988 6.0285 8.5637 7.706 7.8675 6.8275 7.7175 3.2022 6.0964 5.2878 4.5464 4.2959 5.0486
Actin 0.0443 0.0132 0.0367 0.0129 0.0433 0.0286 0.1216 0.016 0.018 0.001 0.0291 0.0111 0.0929 0.0576 0.006 0.0178 0.0108 0.0238
Bud 0.001 0.0005 0.0008 0.0005 0.0012 0.0023 0.0008 0.0009 0.0014 0.0007 0.0011 0.0015 0.0012 0.0011 0.001 0.0013 0.0005 0.0009
Bud Neck 0.0017 0.0013 0.0011 0.0008 0.0015 0.0008 0.0015 0.002 0.0015 0.0008 0.0011 0.0014 0.0013 0.0023 0.0012 0.0012 0.0018 0.0013
Bud Periphery 0.002 0.0014 0.0026 0.001 0.0024 0.0106 0.0011 0.0026 0.0051 0.0019 0.0016 0.006 0.002 0.0022 0.002 0.0024 0.0013 0.0042
Bud Site 0.0044 0.001 0.0024 0.0005 0.0048 0.0008 0.0035 0.0059 0.0029 0.0014 0.0027 0.0008 0.0031 0.0048 0.0044 0.0033 0.001 0.001
Cell Periphery 0.0013 0.0017 0.0021 0.0008 0.0008 0.0017 0.0009 0.0046 0.002 0.0035 0.0009 0.0019 0.0012 0.0051 0.0024 0.005 0.0013 0.0027
Cytoplasm 0.0188 0.0053 0.0038 0.0052 0.0021 0.0022 0.0366 0.0045 0.004 0.0023 0.033 0.0026 0.0339 0.0096 0.0038 0.0032 0.0049 0.0072
Cytoplasmic Foci 0.0402 0.0237 0.0199 0.0267 0.0528 0.0152 0.0574 0.024 0.0277 0.0121 0.0412 0.0189 0.0625 0.0273 0.023 0.0231 0.0293 0.0169
Eisosomes 0.0005 0.0001 0.0005 0.0003 0.0002 0.0002 0.0007 0.0003 0.0002 0 0.0002 0.0001 0.0006 0.001 0.0002 0.0002 0.0008 0.0002
Endoplasmic Reticulum 0.0248 0.0208 0.0124 0.0095 0.0069 0.0203 0.0104 0.0246 0.0308 0.0057 0.0127 0.0106 0.0159 0.0206 0.0143 0.0073 0.0131 0.0149
Endosome 0.4114 0.3823 0.4192 0.5091 0.314 0.4398 0.3612 0.401 0.365 0.506 0.3898 0.401 0.3342 0.3569 0.3934 0.4422 0.4808 0.3413
Golgi 0.1343 0.1261 0.129 0.1301 0.1647 0.1484 0.1381 0.1295 0.1533 0.124 0.1678 0.1207 0.0809 0.1243 0.1341 0.1072 0.2386 0.12
Lipid Particles 0.0662 0.0299 0.0233 0.0489 0.0525 0.0294 0.0736 0.0484 0.0446 0.0142 0.0183 0.0644 0.0747 0.0622 0.0753 0.0509 0.0497 0.086
Mitochondria 0.0549 0.0557 0.0753 0.0129 0.0179 0.0471 0.0719 0.0536 0.0684 0.0433 0.0589 0.0456 0.0439 0.0335 0.0615 0.0464 0.0468 0.0541
None 0.0131 0.0002 0.0005 0.0029 0.0005 0.0003 0.0153 0.0003 0.0005 0.0001 0.0007 0.003 0.0207 0.0004 0.0006 0.0003 0.0016 0.0003
Nuclear Periphery 0.0163 0.006 0.0058 0.004 0.0091 0.0127 0.0125 0.0059 0.0068 0.0022 0.007 0.0156 0.0172 0.0126 0.0045 0.0025 0.0117 0.0084
Nucleolus 0.0007 0.0002 0.0003 0.0002 0.0006 0.0003 0.0004 0.0014 0.0018 0.0004 0.0002 0.0004 0.0016 0.0004 0.001 0.0007 0.0005 0.0005
Nucleus 0.0031 0.0007 0.0007 0.0005 0.0015 0.0013 0.0009 0.0009 0.0021 0.0007 0.0026 0.0008 0.0053 0.0039 0.001 0.0007 0.0025 0.0009
Peroxisomes 0.0043 0.0012 0.0018 0.0008 0.0615 0.0008 0.0244 0.0019 0.0058 0.0005 0.0057 0.0019 0.0227 0.003 0.003 0.0081 0.001 0.0029
Punctate Nuclear 0.0032 0.0001 0.0002 0.0002 0.0023 0.0002 0.003 0.0002 0.0033 0.0001 0.0021 0.0003 0.0108 0.0003 0.0007 0.0004 0.0013 0.0004
Vacuole 0.0935 0.1893 0.1298 0.1132 0.1221 0.1166 0.0405 0.1799 0.1619 0.1525 0.1276 0.134 0.107 0.1982 0.1748 0.2189 0.0631 0.1749
Vacuole Periphery 0.06 0.1393 0.1318 0.119 0.1371 0.1204 0.0235 0.0917 0.0931 0.1265 0.0957 0.1572 0.0664 0.0728 0.0917 0.0567 0.0374 0.1373

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.7845 25.7316 22.2762 35.7016 25.9169 25.572 29.2693 28.2908 26.4798 36.8128
Translational Efficiency 2.2294 2.4346 2.3976 1.1813 2.3555 3.1876 2.2638 2.3918 2.567 1.951

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in late endosome to vacuole transport; cytoplasmic and vacuolar membrane protein; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p
Localization
Cell Percentages vacuolar membrane (34%), ER (9%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps60

Vps60


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps60-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available