Standard name
Human Ortholog
Description Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc; IZH1 has a paralog, IZH4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.94 0.95 0.98 0.99 0.92 0.85 0.79 0.75 0.72 0.65 0.62 0.55 0.99 0.98 0.98 0.97 – 0.97 0.65 0.67 0.61 0.55 0.65 0.63
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 – 0 0.12 0.06 0.1 0.05 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0.15 0.3 0.49 0.43 0.5 0.5 0.62 0.63 0 0 0 0 – 0 0.09 0.09 0.15 0.21 0.14 0.13
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – 0 2 18 12 9 12 14
Bud 0 3 1 0 1 0 2 8 5 4 14 16 0 0 0 0 – 0 2 15 1 4 10 19
Bud Neck 0 2 1 1 2 2 3 2 1 2 1 1 5 3 2 2 – 1 0 7 6 8 8 9
Bud Site 0 0 0 0 0 0 1 2 1 1 4 3 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 3 0 0 2 0 0 0 0 0 0 2 4 5 3 6 – 4 0 8 5 10 15 10
Cytoplasm 45 539 329 270 239 265 254 453 269 244 229 303 543 461 606 186 – 128 42 394 212 221 383 363
Endoplasmic Reticulum 2 6 2 0 1 2 0 0 1 2 1 1 1 7 2 13 – 5 7 34 33 20 17 10
Endosome 0 4 0 0 2 0 0 0 0 2 0 0 0 0 4 0 – 0 0 9 3 4 2 14
Golgi 0 3 0 0 0 0 0 0 0 0 0 0 1 0 1 0 – 0 0 9 2 2 3 2
Mitochondria 5 18 8 8 38 92 156 258 188 188 230 345 0 1 0 4 – 5 5 50 52 84 83 76
Nucleus 0 0 0 0 0 2 1 2 3 1 1 2 0 0 1 0 – 0 0 0 0 1 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 – 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 1 0 0 2 0 0 0 1 0 0 0 1 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 0 2 10 3 10 12 13 0 0 2 0 – 0 0 7 2 5 5 16
Vac/Vac Membrane 1 7 1 0 1 0 1 2 6 2 1 2 4 1 5 1 – 3 1 11 7 20 33 26
Unique Cell Count 48 569 336 274 259 311 321 605 375 373 368 546 550 469 617 191 132 65 585 349 402 586 578
Labelled Cell Count 53 586 343 279 289 363 420 739 478 457 494 689 559 478 627 213 146 65 585 349 402 586 578


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.3 3.6 3.7 3.3 3.1 2.5 2.7 2.6 2.5 2.6 2.5 5.4 4.9 4.9 5.1 4.8 5.1 5.8 5.5 5.4
Std Deviation (1e-4) 2.7 0.9 0.8 1.1 1.4 0.9 0.6 0.7 0.8 0.7 1.1 0.6 1.2 0.8 1.0 1.1 0.9 1.2 1.5 1.1 1.3
Intensity Change (Log2) – – – 0.03 -0.12 -0.19 -0.53 -0.41 -0.47 -0.54 -0.46 -0.5 0.58 0.46 0.47 0.51 0.43 0.52 0.69 0.61 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2098 0.2585 0.0398 0.4169 -0.078 0.1923 2.559 2.9861 2.826 3.004 3.4543 2.8186 1.1643 1.1378 1.1242 1.0314 0.2985 1.5209
Actin 0.0018 0.0006 0.0003 0.0001 0.0002 0.0005 0.0247 0.0011 0.0046 0.0009 0.0243 0.0046 0.018 0.0022 0.0049 0.0013 0.0008 0.0046
Bud 0.0049 0.0099 0.0089 0.0002 0.0034 0.0029 0.0016 0.0026 0.0013 0.0025 0.0027 0.0057 0.0366 0.0872 0.069 0.0321 0.0226 0.0593
Bud Neck 0.0018 0.0035 0.0003 0 0.0006 0.0005 0.0083 0.0007 0.0014 0.0017 0.051 0.0076 0.0044 0.0013 0.0058 0.0013 0.001 0.0076
Bud Periphery 0.001 0.0027 0.0019 0 0.0011 0.0006 0.0024 0.0022 0.0017 0.0054 0.0132 0.0062 0.0162 0.0182 0.0184 0.0113 0.0105 0.0288
Bud Site 0.0033 0.0109 0.0028 0 0.0184 0.0004 0.0209 0.0347 0.0096 0.0018 0.004 0.0055 0.0247 0.0808 0.0469 0.0022 0.0033 0.0036
Cell Periphery 0.0002 0.0006 0.0002 0 0.0001 0.0001 0.0008 0.0013 0.0012 0.0006 0.0005 0.0004 0.0016 0.0015 0.0014 0.0004 0.0012 0.0013
Cytoplasm 0.0146 0.0239 0.019 0.0237 0.0429 0.0334 0.0811 0.1679 0.1059 0.2358 0.0874 0.1665 0.1112 0.1361 0.1193 0.1606 0.1568 0.2534
Cytoplasmic Foci 0.0048 0.0036 0.0029 0.001 0.0055 0.0058 0.0156 0.0062 0.0106 0.0086 0.0192 0.009 0.0567 0.0129 0.0205 0.0137 0.0225 0.0343
Eisosomes 0.0002 0.0002 0.0002 0 0.0001 0.0001 0.0003 0.0002 0.001 0.0001 0.0005 0.0002 0.0006 0.0002 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0002 0.0007 0.0003 0.0001 0.0007 0.0004 0.0083 0.0059 0.0067 0.0145 0.0069 0.0103 0.0056 0.0041 0.0031 0.0024 0.0049 0.0046
Endosome 0.0002 0.0008 0.0002 0.0001 0.0017 0.0003 0.0095 0.0019 0.0052 0.0027 0.0198 0.0032 0.0093 0.0014 0.0042 0.0038 0.0035 0.0034
Golgi 0.0001 0.0001 0 0 0.0001 0 0.0025 0.0002 0.0011 0.0005 0.0035 0.0004 0.0027 0.0002 0.0023 0.0001 0.0002 0.0008
Lipid Particles 0.0002 0.0004 0.0002 0 0.0002 0.0001 0.0057 0.0005 0.0016 0.0012 0.0023 0.0015 0.0072 0.0007 0.002 0.0002 0.0015 0.0033
Mitochondria 0.0005 0.0004 0.0006 0.0001 0.0004 0.0003 0.0015 0.0004 0.0089 0.0016 0.0035 0.0009 0.03 0.0078 0.0117 0.0013 0.001 0.0028
None 0.9574 0.9222 0.9329 0.9392 0.9138 0.9315 0.7875 0.7429 0.8208 0.6942 0.7261 0.759 0.6027 0.5424 0.5434 0.6533 0.6755 0.4427
Nuclear Periphery 0.0003 0.0008 0.0012 0.0004 0.0005 0.0006 0.0093 0.0017 0.0032 0.0076 0.0066 0.0032 0.0094 0.0043 0.0056 0.0074 0.011 0.0062
Nucleolus 0.001 0.002 0.0024 0.0007 0.0007 0.0014 0.0011 0.0018 0.0011 0.0014 0.0041 0.0009 0.0116 0.0046 0.007 0.0038 0.0031 0.0043
Nucleus 0.0015 0.0064 0.0106 0.009 0.003 0.005 0.0075 0.0106 0.0039 0.0082 0.0055 0.005 0.0171 0.0419 0.0458 0.0372 0.028 0.0507
Peroxisomes 0.0009 0.0003 0.0004 0 0.0003 0.0001 0.0029 0.0002 0.0014 0.0005 0.0093 0.0004 0.0088 0.0004 0.002 0.0002 0.0003 0.0065
Punctate Nuclear 0.0046 0.0093 0.0143 0.0252 0.0059 0.0157 0.0054 0.0156 0.0069 0.0074 0.0065 0.0083 0.018 0.049 0.0829 0.0642 0.0456 0.0768
Vacuole 0.0004 0.0007 0.0004 0.0001 0.0004 0.0003 0.0023 0.0013 0.0015 0.0023 0.0023 0.001 0.0053 0.0023 0.0028 0.0024 0.0056 0.0041
Vacuole Periphery 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0007 0.0001 0.0005 0.0004 0.0008 0.0003 0.0023 0.0004 0.0007 0.0006 0.0011 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.1442 16.5952 14.6233 16.3134 18.804 22.0327 11.2736 12.3867 9.4215 15.3596
Translational Efficiency 1.064 1.0313 1.4535 0.8881 1.2552 0.8621 1.1145 1.0835 1.2484 1.0573

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1833 2436 1822 1104 2162 2166 270 1459 3995 4602 2092 2563

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 670.88 706.73 769.71 993.46 670.72 729.20 1007.77 1116.11 670.79 717.31 800.43 1063.28
Standard Deviation 77.52 91.13 101.98 159.51 78.65 96.65 137.80 194.57 78.13 94.44 133.71 190.26
Intensity Change Log 2 — 0.075104 0.198260 0.566407 — 0.120604 0.587384 0.734697 — 0.098031 0.405879 0.652994

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000138 0.000489 0.000147 0.006164 0.000190 0.000520 0.005784 0.005531 0.000166 0.000503 0.000874 0.005804
Bud Neck 0.011524 0.026776 0.004847 0.011395 0.015191 0.026625 0.010023 0.007971 0.013508 0.026705 0.005515 0.009446
Bud Site 0.003568 0.012988 0.005190 0.038475 0.003350 0.011638 0.033647 0.035436 0.003450 0.012352 0.008863 0.036745
Cell Periphery 0.000372 0.000199 0.000350 0.003338 0.000212 0.000261 0.001932 0.004200 0.000285 0.000228 0.000554 0.003829
Cytoplasm 0.415396* 0.389180* 0.341800* 0.211143* 0.414986* 0.390904* 0.166261* 0.122901* 0.415174* 0.389991* 0.319144* 0.160911*
Cytoplasmic Foci 0.192333* 0.199230* 0.017033 0.026210 0.212932* 0.192543* 0.020636 0.031670 0.203481* 0.196083* 0.017498 0.029318
Eisosomes 0.000454 0.000281 0.000049 0.000623 0.000377 0.000360 0.000440 0.000666 0.000412 0.000318 0.000099 0.000648
Endoplasmic Reticulum 0.035292 0.021660 0.007540 0.423826* 0.012038 0.029489 0.489601* 0.577010* 0.022708 0.025345 0.069756 0.511027*
Endosome 0.011482 0.018423 0.002481 0.012439 0.009292 0.015601 0.010971 0.027248 0.010297 0.017095 0.003577 0.020869
Golgi 0.004974 0.005727 0.000083 0.004781 0.004141 0.005406 0.001200 0.013011 0.004523 0.005576 0.000227 0.009466
Lipid Particles 0.059036 0.030919 0.002650 0.024267 0.037336 0.040938 0.014296 0.030523 0.047293 0.035634 0.004153 0.027828
Mitochondria 0.009576 0.012009 0.000567 0.001371 0.009569 0.017068 0.000745 0.005093 0.009573 0.014390 0.000590 0.003490
Mitotic Spindle 0.000450 0.002690 0.001827 0.006589 0.001724 0.003748 0.001824 0.011621 0.001140 0.003188 0.001826 0.009454
None 0.003643 0.003972 0.008298 0.001072 0.002405 0.003032 0.000382 0.001739 0.002973 0.003530 0.007277 0.001452
Nuclear Periphery 0.004240 0.003064 0.005579 0.003921 0.002356 0.003541 0.007665 0.004030 0.003221 0.003288 0.005848 0.003983
Nuclear Periphery Foci 0.002325 0.002003 0.003144 0.034307 0.001160 0.001607 0.048612 0.028452 0.001695 0.001817 0.009012 0.030974
Nucleolus 0.001108 0.002034 0.001153 0.000226 0.001396 0.001423 0.000234 0.000315 0.001264 0.001746 0.001034 0.000277
Nucleus 0.188981* 0.177784* 0.567450* 0.069058 0.222151* 0.184801* 0.075021 0.020878 0.206932* 0.181087* 0.503896* 0.041631
Peroxisomes 0.005516 0.009161 0.000342 0.001123 0.005473 0.008921 0.000628 0.002829 0.005493 0.009048 0.000379 0.002094
Vacuole 0.048177 0.079323 0.029223 0.118384 0.042796 0.059538 0.109136 0.065382 0.045265 0.070011 0.039537 0.088213
Vacuole Periphery 0.001414 0.002089 0.000248 0.001288 0.000923 0.002034 0.000961 0.003494 0.001149 0.002063 0.000340 0.002544

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.30 -1.41 -7.35 -6.91 -7.31 -4.09 -2.34 -7.68 -7.18 -0.09 -6.99 -2.35 -10.62 -9.96 -8.21
Bud Neck -8.67 5.36 -0.21 8.28 -5.33 -5.81 2.14 5.33 10.18 1.37 -10.02 8.15 3.77 13.47 -4.67
Bud Site -6.77 -1.51 -10.25 -7.34 -9.62 -6.85 -4.43 -11.72 -8.35 -0.27 -9.66 -4.33 -15.56 -11.05 -11.54
Cell Periphery 7.71 0.62 -7.57 -8.09 -7.60 -3.35 -5.81 -6.86 -6.77 -3.45 4.38 -4.48 -9.38 -9.54 -8.55
Cytoplasm 3.61 10.79 21.58 18.60 11.16 3.18 17.75 39.38 35.21 3.39 4.80 15.71 43.02 38.22 19.34
Cytoplasmic Foci -1.22 42.06 37.77 39.05 -4.63 3.53 40.64 39.62 34.79 -3.74 1.82 61.02 54.31 51.77 -7.43
Eisosomes 4.77 18.01 -1.86 -4.37 -8.55 0.52 -0.53 -2.33 -2.45 -1.65 3.90 17.09 -2.83 -4.05 -7.10
Endoplasmic Reticulum 4.48 10.04 -28.34 -29.99 -31.43* -7.03 -18.52 -50.95* -48.64* -4.32 -1.29 -8.89 -55.66 -55.39* -44.79*
Endosome -6.11 12.57 -0.17 5.45 -10.50 -6.52 -1.47 -10.18 -6.19 -8.00 -9.12 13.89 -9.15 -2.82 -15.86
Golgi -1.30 14.37 0.94 1.58 -4.15 -2.53 7.06 -5.78 -4.90 -7.95 -2.78 17.77 -4.63 -3.51 -9.56
Lipid Particles 10.57 24.15 12.80 3.75 -12.82 -1.44 9.15 2.68 3.86 -5.87 6.59 30.11 10.42 4.79 -17.04
Mitochondria -1.52 7.11 6.45 11.80 -3.85 -4.21 7.51 3.24 7.69 -5.00 -4.10 10.47 6.31 11.92 -5.82
Mitotic Spindle -3.90 -2.03 -3.88 -2.51 -3.00 -2.10 -0.41 -5.42 -4.21 -5.20 -3.58 -1.01 -6.71 -5.01 -5.95
None -0.48 -3.75 4.18 5.38 6.41 -1.33 6.32 1.06 1.92 -2.14 -1.39 -4.27 3.32 4.56 5.78
Nuclear Periphery 1.98 -1.77 0.25 -1.96 2.17 -2.49 -4.28 -2.68 -0.75 2.60 -0.16 -4.60 -1.81 -1.74 2.79
Nuclear Periphery Foci 0.94 -1.62 -15.03 -15.30 -14.34 -2.45 -8.19 -12.76 -12.55 3.10 -0.64 -7.94 -19.11 -19.08 -12.36
Nucleolus -4.67 -0.83 9.00 9.71 4.16 -0.05 4.29 3.83 7.50 -0.66 -2.65 0.28 6.08 11.82 3.90
Nucleus 2.08 -49.34 20.51 19.33 64.32* 6.78 15.13 46.37* 38.91 7.02 6.72 -40.37 46.94 41.39 64.05
Peroxisomes -4.64 8.80 7.40 14.75 -5.21 -4.54 10.75 4.49 8.36 -5.40 -6.58 14.12 8.00 14.98 -7.08
Vacuole -11.30 4.84 -16.53 -11.50 -18.25 -6.44 -7.71 -7.87 -3.77 4.69 -13.05 -1.33 -17.26 -10.38 -14.42
Vacuole Periphery -2.64 5.53 0.76 2.65 -2.61 -7.03 0.03 -4.58 -2.44 -3.06 -6.40 6.25 -3.58 -1.01 -5.99
Standard name
Human Ortholog
Description Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc; IZH1 has a paralog, IZH4, that arose from the whole genome duplication
Localization
Cell Percentages ER (32%), cytoplasm (6%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Izh1

Izh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Izh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available