Standard name | |
---|---|
Human Ortholog | |
Description | Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 4 | 6 | 6 | – | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 3 | 2 | 3 | 8 | 7 | 7 | 16 | 8 | 10 | 5 | 5 | – | 3 | 10 | 10 | 16 | 14 | 0 | 0 | 0 | 0 | 0 | 5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 3 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 2 | 6 | 14 | 22 | 25 | 12 | 20 | 14 | 21 | – | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 23 | 17 | 3 | 20 | 13 | 37 | 53 | 31 | 45 | 50 | 56 | 59 | – | 11 | 18 | 28 | 47 | 29 | 0 | 0 | 0 | 0 | 2 | 4 |
Nucleus | 83 | 62 | 20 | 4 | 30 | 33 | 52 | 62 | 28 | 53 | 45 | 47 | – | 5 | 11 | 53 | 60 | 82 | 50 | 39 | 23 | 4 | 17 | 22 |
Nuclear Periphery | 2 | 0 | 1 | 1 | 1 | 2 | 3 | 3 | 4 | 0 | 3 | 1 | – | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 111 | 90 | 71 | 141 | 295 | 270 | 434 | 554 | 270 | 295 | 314 | 403 | – | 59 | 117 | 173 | 256 | 248 | 124 | 114 | 67 | 8 | 64 | 74 |
Peroxisomes | 0 | 1 | 0 | 3 | 5 | 4 | 9 | 8 | 10 | 5 | 3 | 4 | – | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 5 | 18 | 22 | 39 | 73 | 32 | 39 | 32 | 43 | – | 1 | 7 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 2 |
Vac/Vac Membrane | 1 | 6 | 0 | 0 | 10 | 4 | 7 | 10 | 9 | 6 | 5 | 8 | – | 1 | 1 | 15 | 19 | 19 | 2 | 9 | 4 | 0 | 10 | 17 |
Unique Cell Count | 181 | 148 | 88 | 150 | 336 | 318 | 508 | 656 | 339 | 390 | 391 | 473 | 71 | 142 | 248 | 344 | 345 | 187 | 169 | 98 | 14 | 98 | 135 | |
Labelled Cell Count | 223 | 179 | 98 | 180 | 386 | 394 | 629 | 786 | 422 | 484 | 484 | 601 | 81 | 169 | 285 | 410 | 401 | 187 | 169 | 98 | 14 | 98 | 135 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 25.0 | 31.8 | 17.9 | 25.3 | 21.2 | 17.0 | 15.6 | 15.4 | 14.3 | 13.4 | 12.7 | 13.0 | – | 28.2 | 25.2 | 49.0 | 51.6 | 45.0 | – | 25.2 | 25.3 |
Std Deviation (1e-4) | 7.3 | 8.7 | 5.8 | 7.6 | 6.1 | 5.6 | 4.5 | 4.5 | 4.5 | 4.1 | 3.8 | 4.0 | – | 9.5 | 10.2 | 21.2 | 23.1 | 20.7 | – | 7.8 | 9.9 |
Intensity Change (Log2) | – | – | – | 0.5 | 0.24 | -0.08 | -0.2 | -0.22 | -0.33 | -0.42 | -0.49 | -0.47 | – | 0.65 | 0.49 | 1.45 | 1.53 | 1.33 | – | 0.49 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.5 | 0 | 2.3 | 2.1 | 0 | 2.6 | 2.5 | 2.8 | 2.5 | – | 2.7 | 2.4 | 2.2 | 2.7 | 1.6 |
Nucleus | -5.0 | -3.6 | -3.0 | -3.3 | -3.7 | -3.8 | -2.2 | -2.8 | -3.4 | – | -2.7 | -3.2 | -0.3 | -1.1 | 0.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.2 | 1.7 | 1.0 | 1.1 | 0.9 | -0.2 | -1.0 | -0.1 | 1.1 | – | 0.4 | 0.3 | -2.0 | -1.2 | -1.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2.3 | 2.9 | 2.6 | 2.7 | 2.4 | 2.5 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 21.0527 | 29.8703 | 25.797 | 24.9371 | 17.9406 | 24.9871 | 26.4497 | 31.0149 | 27.098 | 25.6513 | 24.7382 | 26.1274 | 20.3076 | 26.4677 | 23.8489 | 22.0983 | 20.025 | 22.8071 |
Actin | 0.0112 | 0 | 0.0057 | 0.0001 | 0.002 | 0.0015 | 0.0252 | 0 | 0.0076 | 0.0001 | 0.0294 | 0.0016 | 0.0103 | 0 | 0 | 0.043 | 0.0001 | 0.0087 |
Bud | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0008 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 |
Bud Periphery | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0005 | 0.0006 | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0.0002 |
Bud Site | 0.0039 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0065 | 0.0005 | 0.0003 | 0.0072 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 |
Cell Periphery | 0.0005 | 0.0001 | 0.0004 | 0.0004 | 0.0006 | 0.0009 | 0.0003 | 0.0013 | 0.0002 | 0.0006 | 0.0023 | 0.001 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0009 | 0.0007 |
Cytoplasm | 0.0005 | 0 | 0.0002 | 0.0011 | 0.0005 | 0 | 0.0025 | 0 | 0.0008 | 0.0025 | 0.0008 | 0.0001 | 0.0034 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0105 | 0.0001 | 0.0103 | 0.0028 | 0.0074 | 0.001 | 0.0064 | 0.0001 | 0.0066 | 0.0056 | 0.0153 | 0.0045 | 0.0169 | 0 | 0 | 0.0067 | 0.0208 | 0.0005 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0019 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0007 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0013 | 0 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0012 | 0 | 0 | 0 | 0 | 0.0001 |
Endosome | 0.008 | 0 | 0.0019 | 0.0007 | 0.0007 | 0.0009 | 0.0034 | 0 | 0.0062 | 0.0179 | 0.0018 | 0.0037 | 0.0072 | 0 | 0 | 0.0001 | 0.0001 | 0.0012 |
Golgi | 0.0034 | 0 | 0.0018 | 0.0001 | 0.0009 | 0.0013 | 0.0015 | 0 | 0.0042 | 0.0015 | 0.0042 | 0.0031 | 0.0027 | 0 | 0 | 0.0007 | 0.0002 | 0.0016 |
Lipid Particles | 0.0087 | 0.0001 | 0.0087 | 0.0028 | 0.0326 | 0.0055 | 0.0067 | 0.0005 | 0.0029 | 0.0031 | 0.0184 | 0.0117 | 0.0096 | 0.0001 | 0.0001 | 0.0035 | 0.0111 | 0.008 |
Mitochondria | 0.0151 | 0.0004 | 0.0109 | 0.0371 | 0.014 | 0.0318 | 0.0059 | 0.0176 | 0.0023 | 0.0039 | 0.0334 | 0.0531 | 0.0024 | 0.0004 | 0.0004 | 0.0341 | 0.0166 | 0.0173 |
None | 0.0018 | 0 | 0.0011 | 0.0011 | 0.0197 | 0.0001 | 0.0258 | 0 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0042 | 0 | 0 | 0.0006 | 0.0009 | 0.0001 |
Nuclear Periphery | 0.0101 | 0.0001 | 0.0044 | 0.023 | 0.0529 | 0.0222 | 0.0082 | 0.0002 | 0.0029 | 0.0034 | 0.0411 | 0.0145 | 0.0086 | 0.0016 | 0.0008 | 0.0009 | 0.0158 | 0.0138 |
Nucleolus | 0.8461 | 0.9043 | 0.893 | 0.8553 | 0.7601 | 0.8439 | 0.819 | 0.893 | 0.8819 | 0.8036 | 0.6899 | 0.7576 | 0.8397 | 0.8449 | 0.8859 | 0.7806 | 0.7578 | 0.7987 |
Nucleus | 0.0652 | 0.0941 | 0.0493 | 0.0621 | 0.0599 | 0.0722 | 0.087 | 0.0824 | 0.0738 | 0.1311 | 0.1066 | 0.1059 | 0.066 | 0.1513 | 0.1119 | 0.1201 | 0.1515 | 0.1226 |
Peroxisomes | 0.0043 | 0 | 0.0065 | 0.0007 | 0.0232 | 0.0033 | 0.0012 | 0 | 0.0042 | 0.0007 | 0.0082 | 0.0055 | 0.0068 | 0 | 0 | 0.0064 | 0.0009 | 0.0004 |
Punctate Nuclear | 0.0046 | 0.0001 | 0.0012 | 0.005 | 0.0154 | 0.0005 | 0.002 | 0 | 0.0025 | 0.0008 | 0.0017 | 0.0151 | 0.006 | 0.0001 | 0.0001 | 0.0001 | 0.0022 | 0.0022 |
Vacuole | 0.0015 | 0.0002 | 0.0008 | 0.0013 | 0.001 | 0.0017 | 0.0009 | 0.0017 | 0.0012 | 0.0099 | 0.0063 | 0.0017 | 0.0012 | 0.0003 | 0.0001 | 0.0006 | 0.002 | 0.002 |
Vacuole Periphery | 0.002 | 0.0003 | 0.0023 | 0.0056 | 0.0064 | 0.0125 | 0.0017 | 0.0029 | 0.0012 | 0.0067 | 0.0382 | 0.0193 | 0.0049 | 0.0011 | 0.0004 | 0.0009 | 0.0182 | 0.0215 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 80.614 | 83.5742 | 57.5595 | 59.1719 | 62.0585 | 61.5461 | 51.4854 | 40.9818 | 40.0212 | 64.3472 |
Translational Efficiency | 1.9486 | 1.7199 | 2.1297 | 1.513 | 1.8 | 1.5754 | 1.5579 | 1.4103 | 1.3662 | 1.3864 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2324 | 1299 | 2504 | 1177 | 1545 | 1318 | 486 | 1660 | 3869 | 2617 | 2990 | 2837 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1337.47 | 1681.65 | 2123.10 | 3419.22 | 1743.28 | 1891.84 | 2295.13 | 3262.21 | 1499.52 | 1787.51 | 2151.06 | 3327.35 |
Standard Deviation | 330.68 | 492.32 | 492.43 | 1089.24 | 381.92 | 469.88 | 519.34 | 945.01 | 404.27 | 492.49 | 500.94 | 1010.32 |
Intensity Change Log 2 | — | 0.330371 | 0.666666 | 1.354161 | — | 0.117986 | 0.396772 | 0.904045 | — | 0.214052 | 0.520187 | 1.116875 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001128 | 0.000836 | 0.001022 | 0.001057 | 0.000511 | 0.000787 | 0.000562 | 0.001059 | 0.000882 | 0.000811 | 0.000947 | 0.001058 |
Bud Neck | 0.001595 | 0.000879 | 0.002659 | 0.004336 | 0.000676 | 0.001069 | 0.002214 | 0.005485 | 0.001228 | 0.000974 | 0.002587 | 0.005009 |
Bud Site | 0.000648 | 0.000286 | 0.000345 | 0.000978 | 0.000129 | 0.000335 | 0.000573 | 0.001138 | 0.000441 | 0.000311 | 0.000382 | 0.001072 |
Cell Periphery | 0.000083 | 0.000031 | 0.000028 | 0.000106 | 0.000011 | 0.000057 | 0.000019 | 0.000120 | 0.000054 | 0.000044 | 0.000027 | 0.000114 |
Cytoplasm | 0.000588 | 0.000313 | 0.000139 | 0.000247 | 0.000020 | 0.000150 | 0.000074 | 0.000481 | 0.000361 | 0.000231 | 0.000129 | 0.000384 |
Cytoplasmic Foci | 0.001652 | 0.006091 | 0.001100 | 0.008439 | 0.003441 | 0.002928 | 0.004296 | 0.005719 | 0.002366 | 0.004498 | 0.001620 | 0.006847 |
Eisosomes | 0.000322 | 0.000269 | 0.000251 | 0.000255 | 0.000290 | 0.000265 | 0.000216 | 0.000258 | 0.000309 | 0.000267 | 0.000245 | 0.000257 |
Endoplasmic Reticulum | 0.000484 | 0.000248 | 0.000203 | 0.000115 | 0.000109 | 0.000222 | 0.000098 | 0.000151 | 0.000334 | 0.000235 | 0.000186 | 0.000136 |
Endosome | 0.000173 | 0.000036 | 0.000036 | 0.000332 | 0.000013 | 0.000086 | 0.000209 | 0.000188 | 0.000109 | 0.000061 | 0.000064 | 0.000248 |
Golgi | 0.000124 | 0.000145 | 0.000064 | 0.000551 | 0.000077 | 0.000129 | 0.000265 | 0.000232 | 0.000106 | 0.000137 | 0.000096 | 0.000364 |
Lipid Particles | 0.000197 | 0.000124 | 0.000026 | 0.000070 | 0.000031 | 0.000098 | 0.000070 | 0.000051 | 0.000130 | 0.000111 | 0.000033 | 0.000059 |
Mitochondria | 0.003336 | 0.002290 | 0.002438 | 0.005519 | 0.001386 | 0.001546 | 0.002517 | 0.005274 | 0.002557 | 0.001915 | 0.002451 | 0.005375 |
Mitotic Spindle | 0.000252 | 0.000318 | 0.000303 | 0.003131 | 0.000429 | 0.001250 | 0.004356 | 0.004884 | 0.000323 | 0.000787 | 0.000962 | 0.004157 |
None | 0.002421 | 0.001425 | 0.000511 | 0.000883 | 0.000363 | 0.000643 | 0.000454 | 0.000918 | 0.001599 | 0.001031 | 0.000501 | 0.000904 |
Nuclear Periphery | 0.001895 | 0.001168 | 0.002727 | 0.004680 | 0.001479 | 0.001488 | 0.002190 | 0.004097 | 0.001729 | 0.001329 | 0.002640 | 0.004339 |
Nuclear Periphery Foci | 0.001208 | 0.001239 | 0.000702 | 0.001919 | 0.000781 | 0.000978 | 0.000667 | 0.001499 | 0.001037 | 0.001108 | 0.000697 | 0.001674 |
Nucleolus | 0.566615* | 0.649070* | 0.578672* | 0.703841* | 0.743125* | 0.617821* | 0.712471* | 0.662260* | 0.637100* | 0.633332* | 0.600419* | 0.679511* |
Nucleus | 0.385627* | 0.270623* | 0.390042* | 0.199896* | 0.189607* | 0.343046* | 0.186348* | 0.259527* | 0.307351* | 0.307097* | 0.356933* | 0.234787* |
Peroxisomes | 0.022354 | 0.059649 | 0.013316 | 0.042382 | 0.055701 | 0.021502 | 0.080452 | 0.030918 | 0.035670 | 0.040437 | 0.024229 | 0.035674 |
Vacuole | 0.003371 | 0.001607 | 0.000664 | 0.003176 | 0.000484 | 0.001691 | 0.000616 | 0.002907 | 0.002218 | 0.001649 | 0.000656 | 0.003018 |
Vacuole Periphery | 0.005928 | 0.003355 | 0.004752 | 0.018088 | 0.001338 | 0.003911 | 0.001333 | 0.012835 | 0.004095 | 0.003635 | 0.004196 | 0.015015 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 4.93 | 1.89 | 1.13 | -3.56 | -0.53 | -7.69 | -1.07 | -12.13 | -5.54 | -8.50 | 1.88 | -1.67 | -4.15 | -6.32 | -2.57 |
Bud Neck | 4.92 | -5.38 | -10.67 | -15.01 | -6.26 | -3.50 | -6.37 | -14.25 | -12.60 | -8.47 | 2.25 | -8.71 | -16.42 | -17.80 | -9.88 |
Bud Site | 1.81 | 0.86 | -1.93 | -5.29 | -3.37 | -2.68 | -2.05 | -5.21 | -3.93 | -2.14 | 0.96 | -0.20 | -4.20 | -5.88 | -3.97 |
Cell Periphery | 3.74 | 4.16 | -1.44 | -4.78 | -5.11 | -2.96 | -2.49 | -5.81 | -2.57 | -5.30 | 0.77 | 3.40 | -4.23 | -4.72 | -6.93 |
Cytoplasm | 1.04 | 2.15 | 1.53 | 0.33 | -1.46 | -2.61 | -1.96 | -4.13 | -2.75 | -3.59 | 0.85 | 1.76 | -0.35 | -1.57 | -2.92 |
Cytoplasmic Foci | -4.12 | 1.12 | -6.50 | -1.89 | -6.72 | 0.20 | -0.82 | -2.42 | -2.60 | -1.71 | -2.87 | 1.33 | -6.67 | -2.93 | -7.76 |
Eisosomes | 2.39 | 3.26 | 2.31 | 0.49 | -0.23 | 1.19 | 3.76 | 1.38 | 0.37 | -1.78 | 2.67 | 3.99 | 2.76 | 0.61 | -0.65 |
Endoplasmic Reticulum | 3.22 | 5.95 | 8.32 | 2.29 | 6.41 | -3.12 | 0.73 | -2.53 | 1.99 | -3.70 | 2.18 | 4.82 | 7.05 | 2.92 | 4.20 |
Endosome | 2.51 | 2.40 | -0.57 | -1.10 | -1.08 | -2.66 | -1.00 | -1.88 | -0.99 | 0.11 | 1.16 | 0.80 | -1.12 | -1.49 | -1.41 |
Golgi | -0.63 | 3.15 | -1.41 | -1.31 | -1.62 | -1.55 | -1.60 | -6.03 | -1.91 | 0.29 | -1.39 | 0.27 | -2.10 | -1.71 | -2.13 |
Lipid Particles | 0.61 | 2.22 | 1.66 | 0.70 | -2.74 | -1.84 | -0.75 | -1.59 | 1.39 | 0.40 | 0.13 | 2.02 | 1.55 | 1.32 | -1.65 |
Mitochondria | 3.35 | 2.88 | -2.98 | -4.63 | -4.42 | -1.07 | -2.81 | -8.67 | -8.37 | -4.79 | 3.30 | 0.45 | -6.78 | -8.88 | -7.31 |
Mitotic Spindle | -0.52 | -0.94 | -3.57 | -3.50 | -3.45 | -1.18 | -2.67 | -4.07 | -2.71 | -0.32 | -1.30 | -2.68 | -5.32 | -4.14 | -4.17 |
None | 3.00 | 5.76 | 5.11 | 3.07 | -2.27 | -2.54 | -1.18 | -7.24 | -2.17 | -4.58 | 2.83 | 4.98 | 3.71 | 1.04 | -2.94 |
Nuclear Periphery | 1.63 | -1.37 | -3.43 | -4.64 | -2.23 | -0.02 | -0.42 | -3.17 | -3.73 | -1.12 | 1.10 | -1.61 | -4.59 | -5.87 | -2.48 |
Nuclear Periphery Foci | -0.11 | 2.10 | -2.15 | -1.09 | -3.69 | -0.83 | 0.41 | -2.98 | -1.12 | -2.48 | -0.40 | 1.79 | -3.14 | -1.44 | -4.37 |
Nucleolus | -8.67 | -1.31 | -13.84 | -4.88 | -12.45 | 12.58* | 2.80 | 9.71 | -3.54 | 4.37 | 0.57 | 5.20 | -5.43 | -5.42 | -9.75 |
Nucleus | 11.95 | -0.52 | 19.59 | 6.69 | 19.74 | -15.24 | 0.31 | -8.38 | 7.25 | -6.36 | 0.05 | -6.75 | 10.22 | 9.09 | 15.53 |
Peroxisomes | -13.21 | 8.52 | -9.12 | 4.94 | -13.86 | 14.09 | -4.87 | 9.53 | -5.45 | 9.74 | -2.61 | 7.61 | -0.42 | 2.14 | -7.48 |
Vacuole | 3.07 | 5.77 | 0.30 | -3.15 | -6.66 | -2.15 | -0.84 | -5.85 | -1.70 | -5.07 | 1.17 | 5.44 | -2.15 | -3.08 | -8.05 |
Vacuole Periphery | 3.51 | 1.80 | -5.86 | -7.16 | -6.56 | -3.95 | 0.03 | -9.91 | -6.65 | -9.69 | 0.87 | -0.20 | -9.72 | -9.88 | -9.64 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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