Standard name
Human Ortholog
Description High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.13 0 0.13 0.34 0.38 0.28 0.23 0.21 0.13 0.16 0.06 0.08 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0.1 0.11 0.1 0.12 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.89 0.82 0.86 0.77 0.83 0.87 0.74 0.5 0.43 0.31 0.29 0.29 0.85 0.85 0.88 0.84 0.62 0.74 0.68 0.55 0.7 0.58 0.71 0.83
Cytoplasm 0.08 0.25 0.05 0.05 0.1 0.12 0.18 0.24 0.34 0.36 0.39 0.45 0.1 0.11 0.1 0.18 0.37 0.21 0 0.18 0 0.07 0 0
Endoplasmic Reticulum 0.12 0.1 0.13 0.24 0.11 0 0.09 0.24 0.22 0.21 0.21 0.13 0.21 0.23 0.13 0 0 0.06 0.26 0.22 0.22 0.25 0.15 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.12 0 0 0 0.06 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.08 0.12 0.16 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 12 37 9 41 108 175 146 88 94 56 82 12 24 9 4 7 0 1 6 4 1 4 4
Bud 2 5 2 4 5 11 29 51 41 44 52 44 4 3 3 7 2 2 0 1 1 1 4 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2
Bud Site 0 0 0 0 0 0 1 0 6 3 1 3 0 0 0 0 0 0
Cell Periphery 177 197 248 176 261 279 336 264 164 137 125 152 169 261 237 148 113 89 151 174 267 184 375 400
Cytoplasm 17 61 15 12 32 38 81 124 128 161 169 235 19 35 28 31 67 25 5 58 1 23 15 5
Endoplasmic Reticulum 25 25 38 55 36 13 39 127 84 96 91 67 41 69 35 5 5 7 58 69 83 80 77 26
Endosome 0 0 0 0 0 0 0 0 6 6 3 5 0 0 0 0 1 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 2 5 0 3 0 0 1 2 0 4 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 3 13 6 13 18 14 0 0 0 10 14 15 2 2 15 20 39 26
Nucleus 0 0 0 0 0 0 0 1 1 0 4 7 0 0 0 0 0 1 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 1 12 1 8 3 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 2 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 1 5 3 3 4 7 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 2 5 6 36 53 85 0 1 0 0 2 1 0 0 1 2 4 2
Unique Cell Count 200 240 287 229 314 319 456 525 381 449 430 528 198 306 268 177 182 121 223 317 380 319 528 483
Labelled Cell Count 229 300 340 256 377 450 668 750 536 608 581 707 245 393 313 207 211 144 223 317 380 319 528 483


Cell Periphery, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 67.8 56.7 63.7 65.2 48.9 34.3 27.0 21.8 19.0 16.4 14.5 14.0 61.2 62.8 66.9 37.3 45.7 55.0 67.5 69.5 66.3
Std Deviation (1e-4) 15.1 12.9 14.7 12.6 11.9 9.3 8.6 7.5 7.8 7.2 6.6 6.2 13.4 16.5 14.0 10.0 14.3 19.0 13.4 16.5 16.7
Intensity Change (Log2) 0.03 -0.38 -0.89 -1.24 -1.54 -1.74 -1.96 -2.14 -2.18 -0.06 -0.02 0.07 -0.77 -0.48 -0.21 0.08 0.12 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.5 0.1 6.0 7.5 4.9 3.3 2.8 0.1 1.0 -2.5 -2.0 -4.1 -3.9 -3.3 -4.1
Bud 0 0 2.3 3.8 5.0 5.2 5.0 5.7 4.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.8 -1.1 0.4 -4.1 -10.2 -11.4 -14.8 -15.1 -15.7 -0.3 -0.4 0.7 -0.8 -6.1 -3.1
Cytoplasm 0 2.3 2.9 5.0 6.7 8.8 9.5 10.2 11.6 1.9 2.7 2.3 4.3 8.8 4.8
Endoplasmic Reticulum 3.2 -0.7 -4.1 -2.0 3.7 2.9 2.8 2.7 -0.2 2.2 2.9 -0.1 -3.8 -3.8 -2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 2.9 3.5 0 0 0 0 0 4.8 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 4.9 6.2 7.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 118.6286 138.79 129.6599 131.9131 74.5854 134.0688 115.6522 139.9555 136.6626 127.8223 101.9009 122.2285 131.6169 152.578 145.7363 140.4688 140.0306 146.7665
Actin 0.0028 0 0 0.0003 0.0205 0.0001 0.0051 0.0002 0.0001 0.0002 0.015 0.0002 0.0002 0 0 0 0 0
Bud 0.0003 0.0001 0 0.0001 0.0012 0.0002 0.0004 0 0.0005 0.0002 0.0006 0.0003 0.0001 0 0 0 0 0
Bud Neck 0.0006 0.0003 0.0006 0.0033 0.007 0.001 0.0009 0.0005 0.0014 0.0009 0.0029 0.0029 0.0002 0.0003 0.0005 0.0008 0.0009 0.0008
Bud Periphery 0.0019 0.0005 0.0003 0.0008 0.0041 0.0008 0.0034 0.0002 0.002 0.001 0.0039 0.0036 0.0004 0.0001 0.0002 0.0004 0.0007 0.0007
Bud Site 0.001 0.0054 0.0002 0.0003 0.0033 0.0002 0.0018 0.004 0.0004 0.0005 0.0075 0.0003 0.0002 0.0006 0.0002 0 0.0001 0
Cell Periphery 0.5372 0.6472 0.5883 0.6081 0.3678 0.5506 0.5184 0.6376 0.6097 0.5092 0.5339 0.5889 0.5121 0.6039 0.5615 0.5074 0.5187 0.5277
Cytoplasm 0.0038 0.0079 0.01 0.0014 0.0197 0.0044 0.0045 0.0064 0.0014 0.0006 0.0048 0.0025 0.0016 0.0016 0.001 0.0005 0.001 0.0007
Cytoplasmic Foci 0.0029 0 0.0039 0 0.0225 0.0008 0.0092 0.0001 0.0001 0.0002 0.0214 0.0028 0.0002 0 0 0 0 0
Eisosomes 0.0092 0.0055 0.0031 0.0034 0.0118 0.0126 0.0192 0.0076 0.006 0.0094 0.0038 0.0102 0.0101 0.0199 0.0084 0.0054 0.032 0.0064
Endoplasmic Reticulum 0.146 0.0987 0.1347 0.1158 0.1341 0.2136 0.1535 0.1106 0.1098 0.2061 0.1492 0.175 0.2129 0.1025 0.1524 0.2254 0.2436 0.2718
Endosome 0.0006 0 0 0 0.0232 0.0001 0.0019 0 0 0.0001 0.0054 0.0001 0 0 0 0 0 0
Golgi 0.0008 0 0 0.0001 0.0079 0.0001 0.001 0.0003 0 0.0001 0.0042 0.0002 0.0001 0 0 0 0 0.0001
Lipid Particles 0.0034 0.001 0.0018 0.0014 0.0608 0.0025 0.0077 0.0035 0.0006 0.0019 0.0235 0.0053 0.0023 0.0005 0.0006 0.0005 0.0006 0.0011
Mitochondria 0.0026 0 0.0002 0.0001 0.0307 0.0053 0.0059 0 0.0012 0.0227 0.017 0.0103 0.0002 0 0 0 0 0
None 0.0061 0 0.0009 0 0.0142 0.0026 0.0072 0 0.0029 0.001 0.0047 0.0005 0.0009 0 0 0 0 0.0001
Nuclear Periphery 0.2189 0.1988 0.2129 0.2165 0.1181 0.1574 0.2059 0.2062 0.2325 0.2108 0.1211 0.1512 0.2285 0.2477 0.2476 0.2293 0.1835 0.1629
Nucleolus 0.0098 0.0001 0.0015 0.0003 0.0543 0.0045 0.0063 0.0001 0.0004 0.0044 0.0165 0.0087 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0024 0.0005 0.0012 0.001 0.0096 0.001 0.0021 0.0006 0.0008 0.001 0.0023 0.0015 0.0004 0.0004 0.0004 0.0005 0.0004 0.0004
Peroxisomes 0.0008 0 0.0001 0 0.0255 0.0003 0.0059 0 0.0001 0.0004 0.0196 0.0007 0 0 0 0 0 0
Punctate Nuclear 0.0009 0 0.0006 0 0.0184 0 0.0019 0 0 0 0.0023 0.0001 0 0 0 0 0 0
Vacuole 0.0457 0.0337 0.039 0.0464 0.0407 0.0407 0.0352 0.0219 0.0293 0.0274 0.0385 0.0334 0.0289 0.0223 0.0265 0.0291 0.0181 0.0267
Vacuole Periphery 0.0021 0.0001 0.0004 0.0007 0.0047 0.0012 0.0023 0.0002 0.0006 0.0019 0.0018 0.0012 0.0006 0.0001 0.0004 0.0006 0.0003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 137.3716 107.9468 123.0913 158.0525 140.0449 139.51 159.712 154.0892 178.1527 172.6892
Translational Efficiency 1.5522 1.5467 1.4055 1.2564 1.2085 1.6184 1.1951 1.0613 1.0952 1.1179

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (24%), cytoplasm (2%), mixed (61%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gnp1

Gnp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gnp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available