Standard name | |
---|---|
Human Ortholog | |
Description | Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 3 | 0 | 1 | 2 |
Bud | 0 | 4 | 4 | 0 | 0 | 3 | 4 | 12 | 5 | 7 | 2 | 6 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 0 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 4 | 6 | 6 | 6 | 10 | 8 | 21 | 12 | 10 | 24 | 0 | 5 | 4 | 2 | 9 | 4 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 280 | 365 | 310 | 259 | 271 | 356 | 305 | 392 | 312 | 248 | 208 | 250 | 129 | 322 | 288 | 202 | 173 | 149 | 272 | 344 | 264 | 101 | 182 | 198 |
Endoplasmic Reticulum | 2 | 6 | 0 | 0 | 1 | 1 | 4 | 9 | 10 | 6 | 7 | 1 | 0 | 0 | 2 | 9 | 10 | 4 | 0 | 0 | 3 | 0 | 1 | 2 |
Endosome | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 3 | 5 | 2 | 0 | 0 | 0 |
Golgi | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 4 | 0 | 0 | 2 | 0 |
Mitochondria | 26 | 6 | 9 | 17 | 32 | 100 | 122 | 172 | 164 | 152 | 169 | 206 | 0 | 0 | 0 | 2 | 3 | 4 | 9 | 8 | 15 | 1 | 2 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 6 | 8 | 2 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 4 | 4 | 3 | 5 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 3 | 2 | 0 | 1 | 10 | 15 | 25 | 81 | 15 | 29 | 14 | 24 | 0 | 3 | 1 | 4 | 10 | 10 | 5 | 2 | 2 | 1 | 0 | 6 |
Unique Cell Count | 301 | 382 | 314 | 266 | 291 | 410 | 399 | 589 | 451 | 379 | 341 | 414 | 129 | 325 | 291 | 207 | 183 | 162 | 309 | 389 | 303 | 109 | 198 | 230 |
Labelled Cell Count | 312 | 390 | 327 | 285 | 323 | 484 | 477 | 686 | 541 | 464 | 416 | 518 | 129 | 331 | 296 | 225 | 206 | 176 | 309 | 389 | 303 | 109 | 198 | 230 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.9 | 4.2 | 4.3 | 5.3 | 5.4 | 5.9 | 5.7 | 6.3 | 6.1 | 6.1 | 6.3 | 6.3 | 8.2 | 8.7 | 7.1 | 7.9 | 7.6 | 4.2 | 4.6 | 5.2 |
Std Deviation (1e-4) | 0.7 | 1.3 | 1.1 | 1.5 | 1.9 | 2.0 | 2.6 | 2.0 | 2.4 | 2.5 | 2.2 | 2.6 | 2.6 | 3.1 | 3.2 | 2.8 | 2.7 | 2.6 | 1.3 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.32 | 0.35 | 0.48 | 0.43 | 0.58 | 0.52 | 0.53 | 0.56 | 0.56 | 0.95 | 1.04 | 0.74 | 0.9 | 0.83 | -0.01 | 0.12 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 1.2 | 0 | 2.6 | 1.7 | 1.5 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.2 | -3.5 | -5.8 | -8.6 | -11.1 | -10.3 | -11.0 | -11.9 | -12.2 | 1.3 | 0.4 | 0.3 | -1.0 | -2.7 | -3.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 4.0 | 8.0 | 9.5 | 9.4 | 10.9 | 11.6 | 13.4 | 13.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.4 | 4.5 | 6.9 | 3.3 | 5.0 | 3.6 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2922 | 4.2406 | 3.7741 | 2.9508 | 2.8944 | 3.9037 | 5.6449 | 5.2361 | 6.1079 | 5.9671 | 6.7342 | 5.4954 | 6.2988 | 6.8667 | 6.2256 | 6.1958 | 6.5673 | 7.3556 |
Actin | 0.0366 | 0.0008 | 0.0033 | 0.0103 | 0.0028 | 0.0065 | 0.0508 | 0.0031 | 0.0213 | 0.0126 | 0.0325 | 0.0189 | 0.0132 | 0.0034 | 0.0362 | 0.0042 | 0.0372 | 0.0106 |
Bud | 0.0037 | 0.0064 | 0.0054 | 0.0004 | 0.0042 | 0.0004 | 0.0017 | 0.0019 | 0.0034 | 0.0007 | 0.0018 | 0.005 | 0.0012 | 0.0019 | 0.0059 | 0.0025 | 0.0119 | 0.0031 |
Bud Neck | 0.0124 | 0.0002 | 0.0003 | 0.0007 | 0.0007 | 0.001 | 0.0019 | 0.0004 | 0.0008 | 0.0015 | 0.0024 | 0.0012 | 0.0033 | 0.0003 | 0.0055 | 0.0004 | 0.0014 | 0.0018 |
Bud Periphery | 0.0086 | 0.0045 | 0.005 | 0.0006 | 0.0067 | 0.0007 | 0.0027 | 0.0034 | 0.0055 | 0.0008 | 0.0033 | 0.0025 | 0.0014 | 0.0012 | 0.0069 | 0.0041 | 0.0179 | 0.0064 |
Bud Site | 0.0046 | 0.0044 | 0.0065 | 0.0016 | 0.0005 | 0.0006 | 0.0047 | 0.0056 | 0.022 | 0.0021 | 0.0025 | 0.0006 | 0.0023 | 0.0082 | 0.0208 | 0.0015 | 0.0025 | 0.0007 |
Cell Periphery | 0.0009 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0005 | 0.0007 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0001 |
Cytoplasm | 0.6023 | 0.7484 | 0.6265 | 0.6534 | 0.6091 | 0.7462 | 0.6244 | 0.7032 | 0.7117 | 0.5928 | 0.6631 | 0.7485 | 0.6785 | 0.8104 | 0.6508 | 0.609 | 0.6075 | 0.763 |
Cytoplasmic Foci | 0.0235 | 0.0076 | 0.0125 | 0.0484 | 0.0189 | 0.0138 | 0.039 | 0.0065 | 0.0114 | 0.0306 | 0.0493 | 0.019 | 0.026 | 0.0128 | 0.0178 | 0.0105 | 0.0236 | 0.0118 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0062 | 0.0025 | 0.0031 | 0.0034 | 0.003 | 0.0024 | 0.013 | 0.0019 | 0.0042 | 0.0042 | 0.0049 | 0.0032 | 0.008 | 0.0022 | 0.0057 | 0.0028 | 0.0071 | 0.0049 |
Endosome | 0.0141 | 0.0029 | 0.0032 | 0.0131 | 0.0056 | 0.0073 | 0.0263 | 0.0021 | 0.0187 | 0.0312 | 0.0112 | 0.0131 | 0.0186 | 0.0069 | 0.0081 | 0.017 | 0.0107 | 0.0119 |
Golgi | 0.0061 | 0.0003 | 0.0008 | 0.0026 | 0.0005 | 0.0017 | 0.0158 | 0.0005 | 0.0043 | 0.0259 | 0.004 | 0.0066 | 0.0046 | 0.0047 | 0.0049 | 0.0033 | 0.0037 | 0.0035 |
Lipid Particles | 0.0063 | 0.0001 | 0.0012 | 0.0028 | 0.0003 | 0.0041 | 0.0115 | 0.0002 | 0.003 | 0.0131 | 0.0243 | 0.0023 | 0.0096 | 0.0009 | 0.0033 | 0.0055 | 0.0041 | 0.0007 |
Mitochondria | 0.002 | 0.0005 | 0.0058 | 0.0014 | 0.0014 | 0.0029 | 0.0063 | 0.0005 | 0.012 | 0.0131 | 0.0016 | 0.0035 | 0.002 | 0.0005 | 0.0095 | 0.0014 | 0.0084 | 0.0021 |
None | 0.2469 | 0.2134 | 0.3165 | 0.2437 | 0.3365 | 0.2011 | 0.1557 | 0.2621 | 0.1655 | 0.2094 | 0.1683 | 0.1626 | 0.1878 | 0.1409 | 0.2054 | 0.316 | 0.2268 | 0.166 |
Nuclear Periphery | 0.0049 | 0.0009 | 0.0012 | 0.0007 | 0.0013 | 0.0025 | 0.0097 | 0.0007 | 0.0026 | 0.0012 | 0.001 | 0.0012 | 0.0125 | 0.0006 | 0.0024 | 0.0036 | 0.0066 | 0.0026 |
Nucleolus | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0.0007 |
Nucleus | 0.004 | 0.0022 | 0.0019 | 0.0013 | 0.002 | 0.0018 | 0.0093 | 0.0016 | 0.0018 | 0.0013 | 0.0013 | 0.0014 | 0.0036 | 0.0017 | 0.0022 | 0.0026 | 0.0106 | 0.003 |
Peroxisomes | 0.0074 | 0.0002 | 0.0011 | 0.0121 | 0.0014 | 0.003 | 0.0107 | 0.002 | 0.0059 | 0.0538 | 0.0134 | 0.0051 | 0.0127 | 0.0006 | 0.0091 | 0.0033 | 0.0121 | 0.0018 |
Punctate Nuclear | 0.0037 | 0.0007 | 0.0025 | 0.0007 | 0.0015 | 0.0019 | 0.0096 | 0.0015 | 0.0014 | 0.0013 | 0.0114 | 0.0032 | 0.0104 | 0.0007 | 0.002 | 0.0087 | 0.0037 | 0.0034 |
Vacuole | 0.0038 | 0.0029 | 0.0019 | 0.002 | 0.0027 | 0.0011 | 0.0037 | 0.0017 | 0.0025 | 0.0029 | 0.0026 | 0.0012 | 0.0023 | 0.0014 | 0.0022 | 0.0022 | 0.0024 | 0.0014 |
Vacuole Periphery | 0.0007 | 0.0003 | 0.0003 | 0.0004 | 0.0008 | 0.0003 | 0.0016 | 0.0001 | 0.0008 | 0.0009 | 0.0005 | 0.0003 | 0.0009 | 0.0001 | 0.0005 | 0.0006 | 0.0007 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.175 | 21.0007 | 32.1408 | 65.197 | 48.5437 | 60.8994 | 37.1591 | 60.4605 | 103.0956 | 64.1658 |
Translational Efficiency | 1.7321 | 1.3143 | 1.3152 | 1.7284 | 1.508 | 1.8653 | 1.6803 | 1.4387 | 1.5448 | 1.5594 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
551 | 1093 | 122 | 1904 | 258 | 1592 | 1038 | 475 | 809 | 2685 | 1160 | 2379 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 668.16 | 688.69 | 1023.99 | 1035.52 | 900.99 | 698.38 | 1264.33 | 969.80 | 742.41 | 694.44 | 1239.05 | 1022.40 |
Standard Deviation | 90.02 | 98.81 | 208.02 | 261.19 | 266.72 | 103.85 | 370.81 | 279.93 | 199.95 | 101.94 | 364.72 | 266.34 |
Intensity Change Log 2 | — | 0.043661 | 0.615936 | 0.632090 | — | -0.367499 | 0.488790 | 0.106176 | — | -0.177943 | 0.544306 | 0.353849 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000053 | 0.000434 | 0.000375 | 0.002834 | 0.000227 | 0.000556 | 0.000198 | 0.000541 | 0.000108 | 0.000507 | 0.000217 | 0.002376 |
Bud Neck | 0.008688 | 0.035731 | 0.001612 | 0.010940 | 0.000940 | 0.048488 | 0.001648 | 0.004833 | 0.006217 | 0.043295 | 0.001644 | 0.009721 |
Bud Site | 0.003815 | 0.023150 | 0.005423 | 0.045020 | 0.004329 | 0.030516 | 0.007280 | 0.031580 | 0.003979 | 0.027518 | 0.007084 | 0.042337 |
Cell Periphery | 0.000145 | 0.000148 | 0.000280 | 0.000191 | 0.000171 | 0.000146 | 0.000114 | 0.000266 | 0.000153 | 0.000147 | 0.000131 | 0.000206 |
Cytoplasm | 0.590827* | 0.396379* | 0.450541* | 0.654680* | 0.785799* | 0.385428* | 0.779828* | 0.584152* | 0.653006* | 0.389886* | 0.745196* | 0.640598* |
Cytoplasmic Foci | 0.176248 | 0.263423* | 0.069867 | 0.016540 | 0.076723 | 0.241820* | 0.011263 | 0.038353 | 0.144508 | 0.250614* | 0.017426 | 0.020895 |
Eisosomes | 0.000129 | 0.000157 | 0.000107 | 0.000020 | 0.000035 | 0.000130 | 0.000011 | 0.000030 | 0.000099 | 0.000141 | 0.000021 | 0.000022 |
Endoplasmic Reticulum | 0.000469 | 0.000759 | 0.002600 | 0.000885 | 0.000585 | 0.000714 | 0.000604 | 0.002370 | 0.000506 | 0.000733 | 0.000814 | 0.001182 |
Endosome | 0.007606 | 0.035286 | 0.014729 | 0.004943 | 0.003719 | 0.039772 | 0.001910 | 0.010597 | 0.006366 | 0.037946 | 0.003258 | 0.006072 |
Golgi | 0.000747 | 0.006202 | 0.000280 | 0.006548 | 0.000440 | 0.008708 | 0.000430 | 0.009734 | 0.000649 | 0.007688 | 0.000414 | 0.007184 |
Lipid Particles | 0.004694 | 0.006559 | 0.004612 | 0.001341 | 0.001465 | 0.005641 | 0.000762 | 0.001668 | 0.003664 | 0.006015 | 0.001166 | 0.001406 |
Mitochondria | 0.002536 | 0.013537 | 0.000194 | 0.003630 | 0.000220 | 0.018958 | 0.000183 | 0.007539 | 0.001798 | 0.016751 | 0.000184 | 0.004411 |
Mitotic Spindle | 0.000516 | 0.004679 | 0.001305 | 0.085018 | 0.000498 | 0.019785 | 0.015685 | 0.046757 | 0.000511 | 0.013636 | 0.014173 | 0.077379 |
None | 0.003936 | 0.004080 | 0.002396 | 0.006706 | 0.008349 | 0.003243 | 0.006771 | 0.006815 | 0.005343 | 0.003584 | 0.006311 | 0.006728 |
Nuclear Periphery | 0.000369 | 0.000359 | 0.000994 | 0.000710 | 0.000158 | 0.000377 | 0.000473 | 0.000899 | 0.000302 | 0.000370 | 0.000528 | 0.000748 |
Nuclear Periphery Foci | 0.000367 | 0.000550 | 0.006670 | 0.001354 | 0.003159 | 0.000391 | 0.003483 | 0.002690 | 0.001258 | 0.000456 | 0.003818 | 0.001621 |
Nucleolus | 0.000813 | 0.002109 | 0.001031 | 0.000514 | 0.000311 | 0.002162 | 0.000152 | 0.001871 | 0.000653 | 0.002141 | 0.000244 | 0.000785 |
Nucleus | 0.152244* | 0.073656 | 0.182607* | 0.083407 | 0.078867 | 0.058201 | 0.098105 | 0.076113 | 0.128843 | 0.064492 | 0.106993 | 0.081951 |
Peroxisomes | 0.002275 | 0.017840 | 0.000670 | 0.002540 | 0.000319 | 0.023188 | 0.000090 | 0.006423 | 0.001651 | 0.021011 | 0.000151 | 0.003315 |
Vacuole | 0.043059 | 0.111366 | 0.253067* | 0.069715 | 0.033321 | 0.108089 | 0.070588 | 0.159086 | 0.039953 | 0.109423 | 0.089780 | 0.087559 |
Vacuole Periphery | 0.000463 | 0.003595 | 0.000638 | 0.002463 | 0.000366 | 0.003686 | 0.000423 | 0.007682 | 0.000432 | 0.003649 | 0.000445 | 0.003505 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -11.03 | -6.57 | -3.72 | -2.21 | -2.31 | -3.16 | -0.09 | -1.83 | 1.60 | -2.78 | -8.53 | -5.11 | -3.75 | -1.83 | -3.00 |
Bud Neck | -10.13 | 6.33 | 1.59 | 11.58 | -7.78 | -19.19 | -5.54 | -6.48 | 17.73 | -3.04 | -19.20 | 5.08 | 0.08 | 20.12 | -7.16 |
Bud Site | -9.08 | -2.10 | -9.89 | 0.31 | -8.75 | -12.47 | -2.50 | -5.90 | 2.28 | -4.89 | -15.23 | -3.02 | -11.50 | 2.49 | -8.95 |
Cell Periphery | -0.04 | -3.14 | 1.61 | 1.94 | 4.07 | 1.59 | 2.04 | -1.00 | -1.76 | -1.95 | 0.85 | 0.61 | 0.96 | 0.36 | 0.49 |
Cytoplasm | 14.61 | 6.39 | -0.25 | -18.50 | -6.84 | 25.11 | 6.52 | 12.84 | -8.92 | 9.41 | 25.05 | 0.12 | 6.67 | -23.63 | 7.22 |
Cytoplasmic Foci | -8.98 | 13.05 | 23.15 | 36.03 | 5.88 | -17.88 | 8.74 | 5.94 | 32.94 | -5.28 | -15.06 | 22.91 | 22.67 | 52.12 | -1.11 |
Eisosomes | -2.33 | 2.30 | 12.34 | 17.63 | 7.88 | -12.36 | 4.39 | 1.84 | 18.07 | -6.82 | -5.18 | 11.66 | 12.17 | 26.11 | 0.87 |
Endoplasmic Reticulum | -2.68 | -6.61 | -2.61 | 0.31 | 5.89 | -0.49 | -0.26 | -1.64 | -1.30 | -1.58 | -2.05 | -3.98 | -2.98 | -0.68 | 0.68 |
Endosome | -11.19 | -2.24 | 3.96 | 16.26 | 6.94 | -19.21 | 4.23 | -1.22 | 16.88 | -3.57 | -18.93 | 3.78 | 3.74 | 25.46 | 0.06 |
Golgi | -9.01 | 3.75 | -1.67 | 5.21 | -2.55 | -9.96 | 1.21 | -1.27 | 2.70 | -1.33 | -12.67 | 3.08 | -2.16 | 6.82 | -2.72 |
Lipid Particles | -2.49 | 0.80 | 5.30 | 9.44 | 4.69 | -7.85 | 1.48 | -1.11 | 5.59 | -2.01 | -4.46 | 5.51 | 4.71 | 11.05 | -0.81 |
Mitochondria | -4.49 | 1.87 | 1.00 | 5.94 | -5.57 | -11.12 | 0.85 | -1.63 | 7.75 | -1.66 | -9.47 | 1.89 | 0.36 | 11.39 | -4.32 |
Mitotic Spindle | -3.95 | -1.48 | -12.48 | -10.81 | -12.15 | -7.59 | -7.57 | -5.47 | -1.60 | -2.29 | -8.27 | -7.54 | -13.63 | -7.79 | -7.12 |
None | -0.14 | 1.40 | -1.53 | -2.17 | -8.03 | 2.58 | 1.57 | 1.92 | -1.36 | 1.08 | 1.45 | 0.29 | -0.35 | -3.59 | -1.62 |
Nuclear Periphery | 0.04 | -6.64 | -7.58 | -9.58 | 0.97 | -7.04 | -15.55 | -8.34 | -5.48 | -1.75 | -1.56 | -8.56 | -10.43 | -11.28 | -3.99 |
Nuclear Periphery Foci | -2.01 | -7.29 | -8.35 | -6.66 | 5.63 | 5.37 | -0.97 | 1.42 | -6.77 | 3.32 | 4.35 | -7.94 | -2.52 | -9.78 | 6.78 |
Nucleolus | -3.30 | 0.17 | 1.48 | 5.73 | 1.96 | -5.28 | 2.71 | -1.23 | 3.00 | -1.58 | -5.03 | 2.22 | 0.88 | 6.61 | -2.77 |
Nucleus | 10.05 | -1.22 | 4.22 | -9.59 | 2.96 | 2.40 | -3.67 | 0.29 | -2.96 | 5.47 | 10.82 | 1.29 | 2.83 | -13.22 | 1.80 |
Peroxisomes | -10.18 | 2.15 | 1.52 | 12.80 | -1.41 | -13.74 | 5.44 | -1.40 | 9.75 | -1.58 | -15.45 | 2.78 | 0.67 | 16.83 | -3.04 |
Vacuole | -13.20 | -10.50 | -22.93 | -9.44 | 4.90 | -11.21 | -13.63 | -17.24 | -12.62 | -8.67 | -18.30 | -18.15 | -28.21 | -15.35 | -3.53 |
Vacuole Periphery | -7.52 | -0.25 | -1.56 | 6.36 | -1.23 | -10.82 | 1.14 | -2.07 | 1.32 | -2.19 | -12.93 | 1.73 | -2.64 | 7.56 | -3.15 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|