Standard name | |
---|---|
Human Ortholog | |
Description | Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 3 | 0 | 1 | 2 |
Bud | 0 | 4 | 4 | 0 | 0 | 3 | 4 | 12 | 5 | 7 | 2 | 6 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 0 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 4 | 6 | 6 | 6 | 10 | 8 | 21 | 12 | 10 | 24 | 0 | 5 | 4 | 2 | 9 | 4 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 280 | 365 | 310 | 259 | 271 | 356 | 305 | 392 | 312 | 248 | 208 | 250 | 129 | 322 | 288 | 202 | 173 | 149 | 272 | 344 | 264 | 101 | 182 | 198 |
Endoplasmic Reticulum | 2 | 6 | 0 | 0 | 1 | 1 | 4 | 9 | 10 | 6 | 7 | 1 | 0 | 0 | 2 | 9 | 10 | 4 | 0 | 0 | 3 | 0 | 1 | 2 |
Endosome | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 3 | 5 | 2 | 0 | 0 | 0 |
Golgi | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 4 | 0 | 0 | 2 | 0 |
Mitochondria | 26 | 6 | 9 | 17 | 32 | 100 | 122 | 172 | 164 | 152 | 169 | 206 | 0 | 0 | 0 | 2 | 3 | 4 | 9 | 8 | 15 | 1 | 2 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 6 | 8 | 2 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 4 | 4 | 3 | 5 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 3 | 2 | 0 | 1 | 10 | 15 | 25 | 81 | 15 | 29 | 14 | 24 | 0 | 3 | 1 | 4 | 10 | 10 | 5 | 2 | 2 | 1 | 0 | 6 |
Unique Cell Count | 301 | 382 | 314 | 266 | 291 | 410 | 399 | 589 | 451 | 379 | 341 | 414 | 129 | 325 | 291 | 207 | 183 | 162 | 309 | 389 | 303 | 109 | 198 | 230 |
Labelled Cell Count | 312 | 390 | 327 | 285 | 323 | 484 | 477 | 686 | 541 | 464 | 416 | 518 | 129 | 331 | 296 | 225 | 206 | 176 | 309 | 389 | 303 | 109 | 198 | 230 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.9 | 4.2 | 4.3 | 5.3 | 5.4 | 5.9 | 5.7 | 6.3 | 6.1 | 6.1 | 6.3 | 6.3 | 8.2 | 8.7 | 7.1 | 7.9 | 7.6 | 4.2 | 4.6 | 5.2 |
Std Deviation (1e-4) | 0.7 | 1.3 | 1.1 | 1.5 | 1.9 | 2.0 | 2.6 | 2.0 | 2.4 | 2.5 | 2.2 | 2.6 | 2.6 | 3.1 | 3.2 | 2.8 | 2.7 | 2.6 | 1.3 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.32 | 0.35 | 0.48 | 0.43 | 0.58 | 0.52 | 0.53 | 0.56 | 0.56 | 0.95 | 1.04 | 0.74 | 0.9 | 0.83 | -0.01 | 0.12 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 1.2 | 0 | 2.6 | 1.7 | 1.5 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.2 | -3.5 | -5.8 | -8.6 | -11.1 | -10.3 | -11.0 | -11.9 | -12.2 | 1.3 | 0.4 | 0.3 | -1.0 | -2.7 | -3.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 4.0 | 8.0 | 9.5 | 9.4 | 10.9 | 11.6 | 13.4 | 13.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.4 | 4.5 | 6.9 | 3.3 | 5.0 | 3.6 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2922 | 4.2406 | 3.7741 | 2.9508 | 2.8944 | 3.9037 | 5.6449 | 5.2361 | 6.1079 | 5.9671 | 6.7342 | 5.4954 | 6.2988 | 6.8667 | 6.2256 | 6.1958 | 6.5673 | 7.3556 |
Actin | 0.0366 | 0.0008 | 0.0033 | 0.0103 | 0.0028 | 0.0065 | 0.0508 | 0.0031 | 0.0213 | 0.0126 | 0.0325 | 0.0189 | 0.0132 | 0.0034 | 0.0362 | 0.0042 | 0.0372 | 0.0106 |
Bud | 0.0037 | 0.0064 | 0.0054 | 0.0004 | 0.0042 | 0.0004 | 0.0017 | 0.0019 | 0.0034 | 0.0007 | 0.0018 | 0.005 | 0.0012 | 0.0019 | 0.0059 | 0.0025 | 0.0119 | 0.0031 |
Bud Neck | 0.0124 | 0.0002 | 0.0003 | 0.0007 | 0.0007 | 0.001 | 0.0019 | 0.0004 | 0.0008 | 0.0015 | 0.0024 | 0.0012 | 0.0033 | 0.0003 | 0.0055 | 0.0004 | 0.0014 | 0.0018 |
Bud Periphery | 0.0086 | 0.0045 | 0.005 | 0.0006 | 0.0067 | 0.0007 | 0.0027 | 0.0034 | 0.0055 | 0.0008 | 0.0033 | 0.0025 | 0.0014 | 0.0012 | 0.0069 | 0.0041 | 0.0179 | 0.0064 |
Bud Site | 0.0046 | 0.0044 | 0.0065 | 0.0016 | 0.0005 | 0.0006 | 0.0047 | 0.0056 | 0.022 | 0.0021 | 0.0025 | 0.0006 | 0.0023 | 0.0082 | 0.0208 | 0.0015 | 0.0025 | 0.0007 |
Cell Periphery | 0.0009 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0005 | 0.0007 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0001 |
Cytoplasm | 0.6023 | 0.7484 | 0.6265 | 0.6534 | 0.6091 | 0.7462 | 0.6244 | 0.7032 | 0.7117 | 0.5928 | 0.6631 | 0.7485 | 0.6785 | 0.8104 | 0.6508 | 0.609 | 0.6075 | 0.763 |
Cytoplasmic Foci | 0.0235 | 0.0076 | 0.0125 | 0.0484 | 0.0189 | 0.0138 | 0.039 | 0.0065 | 0.0114 | 0.0306 | 0.0493 | 0.019 | 0.026 | 0.0128 | 0.0178 | 0.0105 | 0.0236 | 0.0118 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0062 | 0.0025 | 0.0031 | 0.0034 | 0.003 | 0.0024 | 0.013 | 0.0019 | 0.0042 | 0.0042 | 0.0049 | 0.0032 | 0.008 | 0.0022 | 0.0057 | 0.0028 | 0.0071 | 0.0049 |
Endosome | 0.0141 | 0.0029 | 0.0032 | 0.0131 | 0.0056 | 0.0073 | 0.0263 | 0.0021 | 0.0187 | 0.0312 | 0.0112 | 0.0131 | 0.0186 | 0.0069 | 0.0081 | 0.017 | 0.0107 | 0.0119 |
Golgi | 0.0061 | 0.0003 | 0.0008 | 0.0026 | 0.0005 | 0.0017 | 0.0158 | 0.0005 | 0.0043 | 0.0259 | 0.004 | 0.0066 | 0.0046 | 0.0047 | 0.0049 | 0.0033 | 0.0037 | 0.0035 |
Lipid Particles | 0.0063 | 0.0001 | 0.0012 | 0.0028 | 0.0003 | 0.0041 | 0.0115 | 0.0002 | 0.003 | 0.0131 | 0.0243 | 0.0023 | 0.0096 | 0.0009 | 0.0033 | 0.0055 | 0.0041 | 0.0007 |
Mitochondria | 0.002 | 0.0005 | 0.0058 | 0.0014 | 0.0014 | 0.0029 | 0.0063 | 0.0005 | 0.012 | 0.0131 | 0.0016 | 0.0035 | 0.002 | 0.0005 | 0.0095 | 0.0014 | 0.0084 | 0.0021 |
None | 0.2469 | 0.2134 | 0.3165 | 0.2437 | 0.3365 | 0.2011 | 0.1557 | 0.2621 | 0.1655 | 0.2094 | 0.1683 | 0.1626 | 0.1878 | 0.1409 | 0.2054 | 0.316 | 0.2268 | 0.166 |
Nuclear Periphery | 0.0049 | 0.0009 | 0.0012 | 0.0007 | 0.0013 | 0.0025 | 0.0097 | 0.0007 | 0.0026 | 0.0012 | 0.001 | 0.0012 | 0.0125 | 0.0006 | 0.0024 | 0.0036 | 0.0066 | 0.0026 |
Nucleolus | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0.0007 |
Nucleus | 0.004 | 0.0022 | 0.0019 | 0.0013 | 0.002 | 0.0018 | 0.0093 | 0.0016 | 0.0018 | 0.0013 | 0.0013 | 0.0014 | 0.0036 | 0.0017 | 0.0022 | 0.0026 | 0.0106 | 0.003 |
Peroxisomes | 0.0074 | 0.0002 | 0.0011 | 0.0121 | 0.0014 | 0.003 | 0.0107 | 0.002 | 0.0059 | 0.0538 | 0.0134 | 0.0051 | 0.0127 | 0.0006 | 0.0091 | 0.0033 | 0.0121 | 0.0018 |
Punctate Nuclear | 0.0037 | 0.0007 | 0.0025 | 0.0007 | 0.0015 | 0.0019 | 0.0096 | 0.0015 | 0.0014 | 0.0013 | 0.0114 | 0.0032 | 0.0104 | 0.0007 | 0.002 | 0.0087 | 0.0037 | 0.0034 |
Vacuole | 0.0038 | 0.0029 | 0.0019 | 0.002 | 0.0027 | 0.0011 | 0.0037 | 0.0017 | 0.0025 | 0.0029 | 0.0026 | 0.0012 | 0.0023 | 0.0014 | 0.0022 | 0.0022 | 0.0024 | 0.0014 |
Vacuole Periphery | 0.0007 | 0.0003 | 0.0003 | 0.0004 | 0.0008 | 0.0003 | 0.0016 | 0.0001 | 0.0008 | 0.0009 | 0.0005 | 0.0003 | 0.0009 | 0.0001 | 0.0005 | 0.0006 | 0.0007 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.175 | 21.0007 | 32.1408 | 65.197 | 48.5437 | 60.8994 | 37.1591 | 60.4605 | 103.0956 | 64.1658 |
Translational Efficiency | 1.7321 | 1.3143 | 1.3152 | 1.7284 | 1.508 | 1.8653 | 1.6803 | 1.4387 | 1.5448 | 1.5594 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|