Standard name | |
---|---|
Human Ortholog | |
Description | Acetylated cis-Golgi protein, homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; required for unconventional protein secretion (UPS) of Acb1p in response to starvation; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 23 | 55 | 64 | 30 | 71 | 49 | 46 | 51 | 34 | 35 | 33 | 68 | 90 | 97 | 76 | 111 | 107 | 127 | 16 | 33 | 19 | 25 | 37 | 29 |
Bud | 9 | 3 | 4 | 5 | 19 | 15 | 24 | 11 | 22 | 8 | 30 | 27 | 10 | 11 | 14 | 16 | 24 | 33 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 3 | 1 | 2 | 0 | 0 | 2 | 2 | 1 | 4 | 1 | 5 | 6 | 6 | 15 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 10 | 10 | 8 | 16 | 12 | 14 | 28 | 7 | 8 | 13 | 7 | 21 | 37 | 47 | 29 | 35 | 25 | 2 | 3 | 2 | 3 | 6 | 8 |
Endoplasmic Reticulum | 6 | 27 | 22 | 11 | 10 | 17 | 12 | 23 | 4 | 5 | 18 | 10 | 39 | 34 | 31 | 12 | 15 | 17 | 2 | 3 | 4 | 6 | 23 | 29 |
Endosome | 17 | 2 | 9 | 6 | 20 | 17 | 13 | 17 | 4 | 15 | 10 | 31 | 11 | 18 | 22 | 7 | 4 | 4 | 14 | 3 | 22 | 14 | 9 | 8 |
Golgi | 54 | 101 | 66 | 66 | 164 | 116 | 59 | 85 | 31 | 33 | 35 | 96 | 109 | 139 | 153 | 222 | 258 | 208 | 113 | 114 | 110 | 102 | 119 | 112 |
Mitochondria | 108 | 45 | 16 | 51 | 72 | 129 | 200 | 152 | 176 | 160 | 261 | 247 | 7 | 6 | 1 | 17 | 42 | 26 | 3 | 1 | 9 | 2 | 2 | 5 |
Nucleus | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 1 | 1 | 1 | 0 | 1 | 0 | 2 | 7 | 7 | 13 | 3 | 7 | 1 | 0 | 0 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 4 | 6 | 1 | 6 | 8 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 |
SpindlePole | 0 | 1 | 2 | 1 | 7 | 2 | 6 | 6 | 6 | 6 | 3 | 11 | 7 | 8 | 3 | 13 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 2 | 2 | 2 | 2 | 3 | 1 | 3 | 1 | 8 | 4 | 7 | 2 | 3 | 5 | 51 | 42 | 13 | 0 | 2 | 1 | 0 | 0 | 1 |
Unique Cell Count | 135 | 138 | 113 | 105 | 225 | 214 | 260 | 255 | 202 | 203 | 297 | 343 | 195 | 228 | 250 | 302 | 339 | 288 | 160 | 167 | 174 | 158 | 206 | 203 |
Labelled Cell Count | 228 | 249 | 199 | 184 | 385 | 363 | 379 | 392 | 302 | 297 | 420 | 526 | 298 | 359 | 359 | 488 | 549 | 470 | 160 | 167 | 174 | 158 | 206 | 203 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 15.7 | 11.5 | 10.7 | 11.1 | 10.0 | 9.3 | 9.7 | 8.8 | 8.7 | 8.7 | 9.0 | 12.8 | 13.5 | 13.7 | 20.3 | 20.4 | 19.2 | 12.2 | 13.1 | 14.3 |
Std Deviation (1e-4) | 1.9 | 2.3 | 2.0 | 1.8 | 1.6 | 1.7 | 1.9 | 1.8 | 1.9 | 1.6 | 1.9 | 1.6 | 2.2 | 1.9 | 2.2 | 4.8 | 5.1 | 5.0 | 2.0 | 2.1 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.06 | -0.2 | -0.31 | -0.24 | -0.39 | -0.41 | -0.4 | -0.35 | 0.16 | 0.23 | 0.25 | 0.82 | 0.83 | 0.74 | 0.08 | 0.19 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -4.2 | -4.4 | -6.1 | -7.6 | -7.0 | -7.3 | -7.2 | -9.7 | -7.5 | -1.8 | -2.5 | -4.8 | -3.7 | -4.8 | -2.3 |
Bud | 0 | 1.7 | 1.3 | 1.9 | 0 | 2.3 | 0 | 2.2 | 1.6 | 0.6 | 0 | 0.8 | 0.7 | 1.4 | 2.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | -0.6 | -1.1 | -1.3 | 0.6 | -2.0 | -1.8 | -1.8 | 0 | 0.5 | 1.9 | 2.4 | 0.2 | 0.5 | -0.1 |
Endoplasmic Reticulum | -1.9 | -4.5 | -3.1 | -4.6 | -2.8 | -5.4 | -5.2 | -4.1 | -6.0 | 0.1 | -1.1 | -1.8 | -5.1 | -5.1 | -4.1 |
Endosome | -0.7 | 0.3 | 0 | -1.1 | -0.4 | 0 | -0.2 | -2.0 | 0.3 | -0.8 | 0 | 0.3 | 0 | 0 | 0 |
Golgi | 0.7 | 2.7 | -0.7 | -6.7 | -4.5 | -7.9 | -7.7 | -9.8 | -5.9 | -0.4 | 0.5 | 0.5 | 3.0 | 3.6 | 2.7 |
Mitochondria | 5.5 | 3.5 | 8.0 | 11.3 | 8.1 | 12.7 | 11.1 | 14.2 | 10.8 | -3.4 | -4.1 | -5.7 | -2.9 | -0.5 | -1.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 3.3 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.7276 | 11.1645 | 10.3744 | 10.4507 | 9.6376 | 10.5666 | 9.4457 | 10.704 | 9.756 | 9.3168 | 9.4201 | 10.0484 | 8.8656 | 9.9701 | 8.9264 | 8.7558 | 8.4534 | 9.112 |
Actin | 0.0732 | 0.0659 | 0.0768 | 0.0046 | 0.0321 | 0.056 | 0.0659 | 0.0201 | 0.0777 | 0.0249 | 0.048 | 0.0248 | 0.0678 | 0.0169 | 0.0608 | 0.0259 | 0.0035 | 0.0697 |
Bud | 0.0003 | 0.0004 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Bud Neck | 0.0005 | 0.0002 | 0.0018 | 0.0002 | 0.0001 | 0.0004 | 0.0015 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0006 |
Bud Periphery | 0.0002 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Bud Site | 0.0015 | 0.0014 | 0.0006 | 0 | 0 | 0.0001 | 0.0004 | 0.0021 | 0.0021 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0013 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0048 | 0.0032 | 0.002 | 0.0015 | 0.001 | 0.0026 | 0.0042 | 0.0095 | 0.0028 | 0.0014 | 0.0018 | 0.0023 | 0.0013 | 0.0057 | 0.0017 | 0.0015 | 0.0012 | 0.0024 |
Cytoplasmic Foci | 0.0753 | 0.1082 | 0.0567 | 0.0614 | 0.0627 | 0.0525 | 0.0875 | 0.1335 | 0.0872 | 0.097 | 0.0797 | 0.0634 | 0.077 | 0.1209 | 0.0769 | 0.1041 | 0.0817 | 0.0639 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0029 | 0.0004 | 0.0004 | 0.0006 | 0.0003 | 0.0007 | 0.0007 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0005 |
Endosome | 0.1032 | 0.0957 | 0.0602 | 0.076 | 0.0806 | 0.0628 | 0.0847 | 0.1267 | 0.0773 | 0.1022 | 0.0733 | 0.0797 | 0.08 | 0.0971 | 0.0733 | 0.11 | 0.0875 | 0.0646 |
Golgi | 0.7133 | 0.7168 | 0.7687 | 0.852 | 0.8198 | 0.8084 | 0.7284 | 0.6878 | 0.7315 | 0.762 | 0.7839 | 0.8154 | 0.7561 | 0.7478 | 0.7749 | 0.748 | 0.8203 | 0.784 |
Lipid Particles | 0.0083 | 0.0012 | 0.0016 | 0.0011 | 0.0007 | 0.0017 | 0.0067 | 0.0011 | 0.0035 | 0.0028 | 0.0022 | 0.001 | 0.0028 | 0.005 | 0.0027 | 0.0025 | 0.0009 | 0.0017 |
Mitochondria | 0.0068 | 0.0011 | 0.0031 | 0.001 | 0.0009 | 0.0028 | 0.0028 | 0.0014 | 0.0022 | 0.0013 | 0.0019 | 0.0073 | 0.0048 | 0.0008 | 0.002 | 0.0008 | 0.0013 | 0.0029 |
None | 0.0005 | 0 | 0.0007 | 0 | 0 | 0.0006 | 0.0044 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.0007 | 0 | 0.0001 | 0 | 0 | 0.0013 | 0.0004 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0.0007 |
Nucleolus | 0.0001 | 0 | 0.0009 | 0 | 0 | 0.0002 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0003 | 0.0008 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 |
Peroxisomes | 0.0053 | 0.0047 | 0.0243 | 0.001 | 0.0012 | 0.0071 | 0.0077 | 0.0106 | 0.0135 | 0.0071 | 0.0072 | 0.0034 | 0.0062 | 0.0034 | 0.0063 | 0.006 | 0.0021 | 0.0076 |
Punctate Nuclear | 0.0006 | 0.0001 | 0.0002 | 0 | 0 | 0.0017 | 0.0026 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 |
Vacuole | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0042 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 |
Vacuole Periphery | 0.001 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0.0017 | 0.0003 | 0.0003 | 0.0003 | 0.0006 | 0.0006 | 0.0002 | 0.0003 | 0.0004 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.8937 | 34.3777 | 35.3212 | 57.1842 | 54.9744 | 36.4641 | 44.1175 | 45.1157 | 61.8581 | 64.9534 |
Translational Efficiency | 1.1761 | 1.1136 | 1.2206 | 1.0482 | 1.0913 | 1.4117 | 0.9697 | 1.0957 | 1.0975 | 0.9076 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
21 | 1299 | 232 | 2030 | 1313 | 1799 | 795 | 1646 | 1334 | 3098 | 1027 | 3676 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1276.83 | 1210.06 | 1470.21 | 1505.33 | 1148.44 | 1211.22 | 1557.77 | 1485.65 | 1150.46 | 1210.73 | 1537.99 | 1496.52 |
Standard Deviation | 218.69 | 183.79 | 195.14 | 218.83 | 173.73 | 163.38 | 201.65 | 233.97 | 175.26 | 172.23 | 203.52 | 225.95 |
Intensity Change Log 2 | — | -0.077488 | 0.203456 | 0.237513 | — | 0.076785 | 0.439807 | 0.371419 | — | -0.002375 | 0.320210 | 0.302473 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.027988 | 0.060320 | 0.038450 | 0.079031 | 0.046327 | 0.075366 | 0.079632 | 0.088302 | 0.046038 | 0.069058 | 0.070329 | 0.083182 |
Bud Neck | 0.003805 | 0.000161 | 0.000916 | 0.000410 | 0.000188 | 0.000163 | 0.000708 | 0.000597 | 0.000245 | 0.000162 | 0.000755 | 0.000494 |
Bud Site | 0.008123 | 0.001254 | 0.003237 | 0.004481 | 0.001243 | 0.002041 | 0.002290 | 0.007317 | 0.001352 | 0.001711 | 0.002504 | 0.005750 |
Cell Periphery | 0.001194 | 0.000198 | 0.000313 | 0.000707 | 0.000203 | 0.000172 | 0.000260 | 0.000594 | 0.000219 | 0.000183 | 0.000272 | 0.000656 |
Cytoplasm | 0.087299 | 0.000735 | 0.000296 | 0.001625 | 0.000551 | 0.000929 | 0.000429 | 0.003114 | 0.001917 | 0.000848 | 0.000399 | 0.002292 |
Cytoplasmic Foci | 0.099879 | 0.185392* | 0.133600 | 0.101444 | 0.210483* | 0.204891* | 0.096103 | 0.132397 | 0.208742* | 0.196715* | 0.104574 | 0.115304 |
Eisosomes | 0.000608 | 0.000388 | 0.000278 | 0.000620 | 0.000293 | 0.000416 | 0.000366 | 0.000378 | 0.000298 | 0.000404 | 0.000346 | 0.000512 |
Endoplasmic Reticulum | 0.070156 | 0.004923 | 0.010314 | 0.008188 | 0.004352 | 0.004111 | 0.007690 | 0.003070 | 0.005388 | 0.004451 | 0.008282 | 0.005896 |
Endosome | 0.124881 | 0.171634 | 0.206729* | 0.141612 | 0.151985 | 0.156266 | 0.146919 | 0.152880 | 0.151558 | 0.162710 | 0.160430 | 0.146657 |
Golgi | 0.272230* | 0.505738* | 0.461468* | 0.611805* | 0.532720* | 0.512996* | 0.546657* | 0.558234* | 0.528619* | 0.509953* | 0.527413* | 0.587818* |
Lipid Particles | 0.021440 | 0.062687 | 0.130709* | 0.037821 | 0.046699 | 0.036039 | 0.107958* | 0.038495 | 0.046301 | 0.047213 | 0.113098* | 0.038122 |
Mitochondria | 0.004264 | 0.000627 | 0.000075 | 0.000935 | 0.000807 | 0.001256 | 0.000083 | 0.000651 | 0.000861 | 0.000992 | 0.000081 | 0.000808 |
Mitotic Spindle | 0.000597 | 0.000965 | 0.000905 | 0.001226 | 0.000288 | 0.000693 | 0.002898 | 0.001880 | 0.000292 | 0.000807 | 0.002448 | 0.001519 |
None | 0.001362 | 0.000017 | 0.000010 | 0.000030 | 0.000010 | 0.000015 | 0.000016 | 0.000021 | 0.000031 | 0.000016 | 0.000015 | 0.000026 |
Nuclear Periphery | 0.001480 | 0.000020 | 0.000130 | 0.000057 | 0.000016 | 0.000019 | 0.000084 | 0.000042 | 0.000039 | 0.000019 | 0.000095 | 0.000050 |
Nuclear Periphery Foci | 0.052909 | 0.000144 | 0.001127 | 0.000336 | 0.000187 | 0.000245 | 0.000649 | 0.000289 | 0.001017 | 0.000202 | 0.000757 | 0.000315 |
Nucleolus | 0.024261 | 0.000002 | 0.000001 | 0.000003 | 0.000001 | 0.000013 | 0.000001 | 0.000003 | 0.000382 | 0.000008 | 0.000001 | 0.000003 |
Nucleus | 0.083006 | 0.000017 | 0.000015 | 0.000020 | 0.000010 | 0.000028 | 0.000017 | 0.000019 | 0.001316 | 0.000024 | 0.000017 | 0.000019 |
Peroxisomes | 0.002753 | 0.002442 | 0.000585 | 0.001230 | 0.001172 | 0.002300 | 0.000624 | 0.003614 | 0.001197 | 0.002360 | 0.000615 | 0.002297 |
Vacuole | 0.098750 | 0.001216 | 0.008957 | 0.005778 | 0.001127 | 0.001058 | 0.005171 | 0.005455 | 0.002663 | 0.001124 | 0.006026 | 0.005634 |
Vacuole Periphery | 0.013016 | 0.001120 | 0.001886 | 0.002644 | 0.001340 | 0.000983 | 0.001444 | 0.002649 | 0.001524 | 0.001040 | 0.001544 | 0.002647 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.42 | -1.12 | -5.26 | -3.23 | -5.05 | -5.64 | -4.71 | -6.99 | -2.04 | -1.09 | -5.30 | -4.01 | -8.18 | -3.31 | -2.14 |
Bud Neck | 3.25 | 2.79 | 3.10 | -5.57 | 3.43 | 0.56 | -4.75 | -3.89 | -4.28 | 0.71 | 2.19 | -4.90 | -3.30 | -6.43 | 2.56 |
Bud Site | 2.79 | 2.11 | 1.80 | -6.47 | -1.16 | -2.51 | -2.88 | -8.08 | -6.83 | -6.38 | -1.32 | -2.91 | -9.42 | -8.94 | -6.13 |
Cell Periphery | 2.84 | 2.44 | 1.19 | -7.14 | -4.36 | 1.39 | -2.12 | -3.02 | -3.28 | -2.58 | 1.54 | -1.97 | -6.12 | -6.79 | -5.36 |
Cytoplasm | 2.10 | 2.11 | 2.08 | -3.30 | -5.22 | -5.01 | 2.62 | -6.70 | -5.67 | -7.01 | 1.54 | 2.15 | -0.43 | -6.38 | -8.53 |
Cytoplasmic Foci | -3.96 | -1.74 | -0.53 | 20.56 | 3.30 | 1.17 | 22.74 | 16.22 | 16.64 | -7.89 | 2.74 | 20.66 | 22.62 | 27.52 | -2.75 |
Eisosomes | 1.02 | 1.68 | -0.43 | -4.13 | -5.31 | -4.27 | -2.19 | -2.67 | 1.14 | -0.30 | -4.13 | -1.64 | -6.35 | -3.18 | -4.47 |
Endoplasmic Reticulum | 2.17 | 1.96 | 2.05 | -3.32 | 1.14 | 0.29 | -3.70 | 2.50 | 1.62 | 5.08 | 1.15 | -3.21 | -0.82 | -2.25 | 2.65 |
Endosome | -2.39 | -3.52 | -1.22 | 6.74 | 6.01 | -1.01 | 0.93 | -0.19 | 0.82 | -1.10 | -2.95 | -1.76 | 1.23 | 5.38 | 3.00 |
Golgi | -4.67 | -3.75 | -6.51 | -12.73 | -8.12 | 2.63 | -1.23 | -2.85 | -5.41 | -0.96 | 2.63 | 0.09 | -8.23 | -13.37 | -6.36 |
Lipid Particles | -4.35 | -6.78 | -1.97 | 5.53 | 6.72 | 2.60 | -8.84 | 1.93 | -0.72 | 10.48 | -0.24 | -10.27 | 2.21 | 3.29 | 12.75 |
Mitochondria | 0.94 | 1.11 | 0.85 | -0.75 | -2.82 | -0.67 | 1.66 | 0.32 | 1.09 | -2.91 | -0.27 | 1.78 | 0.09 | 0.50 | -3.83 |
Mitotic Spindle | -0.60 | -0.63 | -1.47 | -0.48 | -0.72 | -0.85 | -1.73 | -3.60 | -2.08 | 0.60 | -1.34 | -1.83 | -3.96 | -1.79 | 0.73 |
None | 1.96 | 1.97 | 1.94 | -3.49 | -10.07 | -1.84 | -4.89 | -6.48 | -1.60 | -2.74 | 1.33 | 1.46 | 0.56 | -3.92 | -8.15 |
Nuclear Periphery | 1.91 | 1.77 | 1.86 | -2.72 | 3.58 | -0.38 | -5.87 | -2.98 | -2.14 | 2.91 | 1.39 | -3.51 | -0.82 | -3.39 | 3.54 |
Nuclear Periphery Foci | 2.71 | 2.67 | 2.70 | -3.27 | 2.18 | -0.43 | -2.32 | -1.07 | -0.38 | 1.79 | 2.26 | 0.94 | 2.00 | -1.42 | 2.59 |
Nucleolus | 2.39 | 2.39 | 2.39 | -0.81 | -2.24 | -1.14 | -1.73 | -2.95 | 0.97 | -1.59 | 2.12 | 2.16 | 2.15 | 0.92 | -2.32 |
Nucleus | 3.01 | 3.01 | 3.01 | -0.37 | -2.55 | -1.67 | -6.48 | -3.96 | 0.92 | -1.12 | 2.54 | 2.55 | 2.55 | 0.80 | -2.08 |
Peroxisomes | 0.83 | 2.33 | 1.82 | 3.00 | -4.87 | -4.76 | 4.33 | -4.49 | -2.28 | -5.55 | -4.98 | 4.93 | -4.10 | 0.19 | -6.68 |
Vacuole | 3.14 | 2.95 | 3.02 | -7.53 | 2.15 | -0.18 | -6.54 | -8.85 | -7.92 | -0.48 | 2.45 | -2.95 | -2.89 | -10.60 | 0.45 |
Vacuole Periphery | 2.03 | 1.88 | 1.74 | -4.71 | -2.06 | 1.70 | -0.52 | -2.55 | -3.37 | -2.47 | 2.11 | -0.18 | -3.46 | -5.73 | -3.97 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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