Standard name | |
---|---|
Human Ortholog | |
Description | ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | – | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 2 | 0 | 2 | 2 |
Bud | 1 | – | 4 | 1 | 1 | 4 | 3 | 3 | 4 | 5 | 1 | 3 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 2 | 5 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 |
Bud Site | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | – | 0 | 0 | 0 | 3 | 2 | 3 | 8 | 6 | 5 | 9 | 1 | 4 | 3 | 6 | 4 | 5 | 2 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 295 | – | 102 | 82 | 136 | 198 | 240 | 278 | 201 | 144 | 141 | 199 | 198 | 426 | 371 | 339 | 228 | 173 | 281 | 37 | 88 | 156 | 282 | 280 |
Endoplasmic Reticulum | 6 | – | 0 | 0 | 5 | 2 | 0 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 13 | 19 | 7 | 1 | 0 | 2 | 1 | 2 | 2 |
Endosome | 0 | – | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 5 | 2 | 1 | 5 | 0 | 0 | 0 | 3 | 1 |
Golgi | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 0 | 2 |
Mitochondria | 21 | – | 4 | 6 | 6 | 46 | 93 | 96 | 136 | 83 | 130 | 145 | 1 | 3 | 1 | 10 | 8 | 5 | 6 | 0 | 3 | 3 | 2 | 2 |
Nucleus | 0 | – | 1 | 0 | 2 | 1 | 1 | 4 | 6 | 3 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 0 | – | 0 | 0 | 2 | 3 | 1 | 6 | 2 | 1 | 1 | 1 | 1 | 0 | 1 | 2 | 2 | 3 | 1 | 0 | 5 | 1 | 1 | 2 |
Unique Cell Count | 311 | 105 | 84 | 148 | 217 | 295 | 339 | 289 | 185 | 234 | 280 | 201 | 429 | 375 | 348 | 238 | 182 | 315 | 44 | 112 | 171 | 303 | 312 | |
Labelled Cell Count | 327 | 112 | 90 | 153 | 261 | 343 | 392 | 364 | 243 | 282 | 360 | 204 | 435 | 380 | 377 | 264 | 196 | 315 | 44 | 112 | 171 | 303 | 312 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | – | 4.6 | 4.6 | 4.6 | 4.1 | 3.7 | 4.1 | 3.7 | 3.7 | 3.6 | 3.4 | 5.8 | 5.6 | 5.6 | 5.4 | 5.9 | 6.6 | 5.4 | 5.6 | 6.3 |
Std Deviation (1e-4) | 0.5 | – | 1.0 | 1.4 | 1.1 | 1.3 | 1.2 | 1.3 | 0.9 | 1.6 | 1.1 | 0.7 | 1.2 | 1.0 | 1.4 | 1.4 | 1.6 | 2.3 | 1.2 | 1.1 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.0 | -0.0 | -0.17 | -0.31 | -0.2 | -0.32 | -0.31 | -0.36 | -0.44 | 0.31 | 0.28 | 0.27 | 0.21 | 0.35 | 0.52 | 0.23 | 0.27 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -1.7 | -2.0 | -3.9 | -3.9 | -5.7 | -4.4 | -7.0 | -5.5 | 0.8 | 1.9 | 1.4 | 0.2 | -0.6 | -0.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9043 | 2.0912 | 2.0863 | 1.4659 | 2.1074 | 1.8695 | 1.2946 | 1.7823 | 1.3374 | 1.1946 | 0.762 | 1.2546 | 2.6965 | 2.5322 | 2.2489 | 2.2908 | 2.0435 | 2.3113 |
Actin | 0.0647 | 0.0011 | 0.0279 | 0.0097 | 0.0488 | 0.0202 | 0.0387 | 0.0001 | 0.0083 | 0.0034 | 0.0001 | 0.0136 | 0.0732 | 0.0004 | 0.0083 | 0.0037 | 0.0792 | 0.0038 |
Bud | 0.0016 | 0.0011 | 0.001 | 0.0074 | 0.0011 | 0.0004 | 0.0041 | 0 | 0.0008 | 0.0009 | 0 | 0.0004 | 0.0007 | 0.0001 | 0.0007 | 0.0001 | 0.0008 | 0.0045 |
Bud Neck | 0.0057 | 0.0005 | 0.0044 | 0.027 | 0.0051 | 0.0038 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0005 | 0.0256 | 0.0001 | 0.0003 | 0.0004 | 0.0026 | 0.0067 |
Bud Periphery | 0.0027 | 0.0009 | 0.0023 | 0.0177 | 0.0019 | 0.0009 | 0.0096 | 0 | 0.0005 | 0.0006 | 0 | 0.0006 | 0.0011 | 0.0001 | 0.0017 | 0.0001 | 0.0012 | 0.0007 |
Bud Site | 0.0123 | 0.0209 | 0.0064 | 0.0305 | 0.0043 | 0.0015 | 0.0019 | 0.0005 | 0.0023 | 0.0005 | 0 | 0.0004 | 0.0086 | 0.001 | 0.001 | 0.0002 | 0.0014 | 0.0004 |
Cell Periphery | 0.001 | 0.0006 | 0.0007 | 0.0007 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0004 | 0.0006 | 0.0001 | 0.0004 | 0.0001 |
Cytoplasm | 0.4755 | 0.6841 | 0.6323 | 0.4989 | 0.7221 | 0.6693 | 0.2233 | 0.5726 | 0.4608 | 0.4523 | 0.6937 | 0.5658 | 0.4775 | 0.7626 | 0.6619 | 0.7149 | 0.7241 | 0.8063 |
Cytoplasmic Foci | 0.0227 | 0.0056 | 0.0101 | 0.0097 | 0.0055 | 0.0187 | 0.0419 | 0.0067 | 0.0295 | 0.0654 | 0.0027 | 0.0077 | 0.0204 | 0.0236 | 0.0062 | 0.0054 | 0.0038 | 0.0052 |
Eisosomes | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0006 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0.0011 | 0 | 0.0014 | 0.0001 | 0.0007 | 0 |
Endoplasmic Reticulum | 0.0104 | 0.0028 | 0.0071 | 0.0014 | 0.003 | 0.0054 | 0.0102 | 0.0042 | 0.0034 | 0.0017 | 0.002 | 0.0026 | 0.0085 | 0.002 | 0.0059 | 0.0024 | 0.0045 | 0.002 |
Endosome | 0.0431 | 0.0017 | 0.0101 | 0.0041 | 0.013 | 0.0114 | 0.0477 | 0.003 | 0.0199 | 0.0856 | 0.0011 | 0.0052 | 0.0168 | 0.0021 | 0.0058 | 0.0048 | 0.0126 | 0.0021 |
Golgi | 0.0215 | 0.0002 | 0.0022 | 0.0021 | 0.0084 | 0.0047 | 0.0255 | 0.0002 | 0.022 | 0.0211 | 0.0001 | 0.0052 | 0.0102 | 0.0004 | 0.005 | 0.0004 | 0.0079 | 0.0006 |
Lipid Particles | 0.0227 | 0.0002 | 0.0028 | 0.0008 | 0.0059 | 0.0102 | 0.011 | 0.0003 | 0.0037 | 0.0153 | 0 | 0.0047 | 0.0143 | 0.0004 | 0.002 | 0.0003 | 0.0049 | 0.0007 |
Mitochondria | 0.0123 | 0.0001 | 0.0015 | 0.002 | 0.011 | 0.0027 | 0.0108 | 0 | 0.0056 | 0.0031 | 0 | 0.0047 | 0.0151 | 0.0001 | 0.0021 | 0.0009 | 0.003 | 0.0006 |
None | 0.2662 | 0.2761 | 0.2582 | 0.3782 | 0.1065 | 0.2298 | 0.5298 | 0.4099 | 0.4067 | 0.3102 | 0.2977 | 0.3778 | 0.288 | 0.2039 | 0.2929 | 0.2548 | 0.1401 | 0.1592 |
Nuclear Periphery | 0.0095 | 0.0005 | 0.0083 | 0.0005 | 0.0408 | 0.0081 | 0.0136 | 0.0004 | 0.0049 | 0.0003 | 0.0001 | 0.0003 | 0.0091 | 0.0003 | 0.0009 | 0.0013 | 0.0057 | 0.0007 |
Nucleolus | 0.0017 | 0.0001 | 0.0008 | 0.0016 | 0.0011 | 0.0003 | 0.0006 | 0 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0005 | 0.0004 | 0.0001 |
Nucleus | 0.0029 | 0.0013 | 0.0095 | 0.0018 | 0.0075 | 0.0024 | 0.0128 | 0.0003 | 0.0122 | 0.0004 | 0.0003 | 0.0004 | 0.002 | 0.0006 | 0.0008 | 0.0053 | 0.0016 | 0.0024 |
Peroxisomes | 0.0116 | 0.0001 | 0.0019 | 0.0018 | 0.0029 | 0.0032 | 0.0057 | 0 | 0.0069 | 0.0357 | 0 | 0.0081 | 0.0184 | 0.0006 | 0.0007 | 0.0003 | 0.0009 | 0.0003 |
Punctate Nuclear | 0.0034 | 0.0008 | 0.0067 | 0.0012 | 0.004 | 0.004 | 0.0038 | 0.0002 | 0.0091 | 0.0006 | 0.0001 | 0.0012 | 0.0051 | 0.0008 | 0.0005 | 0.0032 | 0.0022 | 0.003 |
Vacuole | 0.0057 | 0.0011 | 0.005 | 0.0025 | 0.0021 | 0.0014 | 0.0047 | 0.0008 | 0.0015 | 0.0017 | 0.0017 | 0.0004 | 0.0014 | 0.0004 | 0.0009 | 0.0007 | 0.0009 | 0.0003 |
Vacuole Periphery | 0.0021 | 0.0001 | 0.0007 | 0.0003 | 0.004 | 0.0009 | 0.0029 | 0 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0017 | 0 | 0.0003 | 0.0002 | 0.0009 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.9914 | 12.9338 | 14.6488 | 21.1398 | 17.2779 | 5.3275 | 18.2395 | 19.5109 | 14.7918 | 23.967 |
Translational Efficiency | 0.6139 | 0.6946 | 0.5178 | 0.3145 | 0.4745 | 1.5676 | 0.4993 | 0.4177 | 0.5132 | 0.3823 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|