Standard name
Human Ortholog
Description Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0 0 0 0 0 0.05 0 0 0 0.05 0 0 0.09 0.05 0.12 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.94 0.97 0.96 0.87 0.69 0.7 0.65 0.62 0.59 0.58 0.99 0.99 0.99 0.95 0.97 0.91 0.96 0.92 0.92 0.94 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0.11 0.09 0.27 0.48 0.43 0.45 0.47 0.58 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 3 0 2 7 0 3 0 0 0 0 0 0 0 0 3 0 0 1
Bud 2 0 0 1 1 3 11 7 5 10 14 13 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 21 7 10 7 19 10 23 15 8 27 7 8 7 29 12 16 0 0 0 0 0 0
Cytoplasm 146 109 296 262 315 316 380 391 311 349 272 360 141 256 276 304 228 125 140 102 306 114 239 253
Endoplasmic Reticulum 0 0 5 0 1 4 10 4 15 28 3 14 1 1 2 6 9 4 2 2 7 0 2 1
Endosome 0 0 0 0 0 0 1 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1
Golgi 0 0 1 1 1 1 0 0 0 0 0 0 0 0 0 1 2 2 0 0 3 0 0 0
Mitochondria 10 1 9 30 28 98 265 242 215 262 269 333 0 1 0 5 0 2 1 0 3 0 0 2
Nucleus 0 0 1 0 3 1 2 5 3 2 0 3 0 1 1 3 1 2 0 1 0 3 1 1
Nuclear Periphery 0 0 0 0 0 2 2 1 1 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 9 6 1 4 12 21 15 11 13 16 0 0 4 5 3 1 0 0 4 2 3 2
Unique Cell Count 147 109 315 271 327 365 552 559 482 563 462 620 143 258 279 321 235 138 147 111 334 122 253 268
Labelled Cell Count 159 112 342 307 360 437 705 682 592 684 582 772 149 267 291 353 255 152 147 111 334 122 253 268


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 8.2 8.1 7.5 6.7 6.0 5.5 5.6 5.5 5.3 5.1 5.2 9.8 9.6 9.0 15.1 15.4 14.5 8.7 9.6 9.9
Std Deviation (1e-4) 0.9 1.3 1.0 1.5 1.4 1.5 1.0 1.1 1.1 0.9 1.0 1.0 1.2 1.3 1.3 3.0 2.7 2.7 1.1 1.3 1.5
Intensity Change (Log2) – – – -0.12 -0.27 -0.43 -0.57 -0.53 -0.56 -0.63 -0.68 -0.63 0.27 0.24 0.15 0.9 0.93 0.84 0.11 0.23 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.3 -2.1 -3.1 -2.2 -3.7 -1.1 -2.9 -3.6 -1.5 -0.7 -1.9 -2.4 1.1 -0.8 1.8
Cytoplasm 1.5 1.4 -3.2 -8.6 -8.3 -9.5 -10.3 -10.8 -11.3 2.2 3.3 3.2 0.4 1.7 -1.3
Endoplasmic Reticulum 0 0 0 0.2 0 1.3 2.5 0 0.7 0 0 0 0.3 1.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.0 3.1 8.6 13.8 12.7 12.8 13.4 15.8 15.3 0 0 0 -1.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.5 0 -1.7 -0.6 0.7 0.2 -0.9 0 -0.2 0 0 -1.2 -1.1 -1.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9216 6.6533 6.186 5.5951 5.4471 6.0422 4.1049 6.2616 5.5184 5.0838 5.0987 5.4051 4.1622 5.8081 5.7967 5.8099 4.7113 5.3851
Actin 0.0012 0.0005 0.0005 0.0003 0.0002 0.0004 0.0295 0.0001 0.0055 0.009 0.0052 0.0006 0.0008 0.0013 0.0002 0 0.0001 0.0002
Bud 0.0003 0.0004 0.0005 0.0007 0.0002 0.0002 0.0012 0.0002 0.0023 0.0003 0.0002 0.0001 0.0005 0.0004 0.0003 0.0003 0.0002 0.0001
Bud Neck 0.0004 0.0002 0.0003 0.0006 0.0004 0.0009 0.0014 0.0001 0.0007 0.0005 0.0003 0.0005 0.0005 0.0002 0.0002 0.0002 0.0003 0.0005
Bud Periphery 0.0002 0.0001 0.0002 0.0003 0 0.0002 0.0017 0 0.0012 0.0002 0.0001 0.0001 0.0003 0.0002 0.0001 0 0.0001 0
Bud Site 0.0015 0.001 0.0009 0.0003 0.0001 0.0001 0.0083 0.0006 0.0017 0.0005 0.0001 0.0001 0.0034 0.0012 0.0003 0.0001 0.0002 0
Cell Periphery 0.0006 0.0003 0.0001 0.0001 0 0.0002 0.0007 0.0001 0.0001 0.0001 0 0 0.0002 0.0002 0 0 0 0
Cytoplasm 0.8519 0.9542 0.9179 0.9345 0.8841 0.912 0.7498 0.9733 0.8801 0.8575 0.9164 0.9206 0.8805 0.9443 0.9444 0.9428 0.8645 0.9462
Cytoplasmic Foci 0.0138 0.0078 0.0126 0.011 0.0176 0.0115 0.0298 0.0064 0.019 0.0213 0.0134 0.0106 0.0107 0.0085 0.0101 0.0108 0.0252 0.0065
Eisosomes 0 0 0 0 0 0 0.0005 0 0.0001 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0566 0.0095 0.0139 0.0047 0.0067 0.0218 0.0507 0.002 0.01 0.0165 0.0036 0.0232 0.0367 0.009 0.0039 0.0022 0.0032 0.0257
Endosome 0.0356 0.0101 0.0353 0.0305 0.063 0.0334 0.0559 0.0117 0.0398 0.0541 0.0189 0.0304 0.029 0.0118 0.0278 0.0267 0.0762 0.0141
Golgi 0.0037 0.0022 0.002 0.0011 0.0039 0.0027 0.0146 0.0007 0.0058 0.0059 0.0046 0.002 0.0017 0.001 0.0008 0.0003 0.0055 0.0005
Lipid Particles 0.001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0086 0 0.0036 0.004 0.011 0.0031 0.0014 0.0003 0 0 0.0001 0
Mitochondria 0.0015 0.0011 0.0011 0.0005 0.0035 0.0042 0.0132 0.0002 0.0053 0.0023 0.0008 0.0016 0.0025 0.0003 0.0003 0.0002 0.013 0.0006
None 0.0133 0.0053 0.0018 0.0012 0.0011 0.0039 0.0055 0.0016 0.0025 0.0032 0.0011 0.0009 0.0142 0.0131 0.0022 0.001 0.0012 0.001
Nuclear Periphery 0.0041 0.0008 0.0015 0.0011 0.0011 0.0009 0.0076 0.0004 0.0069 0.0089 0.0014 0.0008 0.0037 0.0011 0.0008 0.001 0.0008 0.001
Nucleolus 0.0001 0.0002 0 0 0 0 0.001 0 0.001 0.0002 0.0001 0 0.0002 0.0001 0 0 0 0
Nucleus 0.0021 0.0012 0.0011 0.0012 0.0011 0.0009 0.0025 0.0005 0.0022 0.004 0.0006 0.0008 0.0019 0.0013 0.001 0.0015 0.0011 0.001
Peroxisomes 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.004 0 0.0034 0.0009 0.0119 0.0009 0.0023 0.0001 0 0 0.0001 0
Punctate Nuclear 0.0011 0.0008 0.0002 0.0002 0.0002 0.0001 0.0036 0.0001 0.0007 0.0018 0.006 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001
Vacuole 0.0093 0.0035 0.008 0.0089 0.0128 0.0046 0.008 0.0018 0.0064 0.0066 0.0033 0.0025 0.0077 0.005 0.0062 0.0105 0.0057 0.0019
Vacuole Periphery 0.0014 0.0005 0.0015 0.0024 0.0037 0.0016 0.002 0.0003 0.0018 0.002 0.001 0.001 0.0014 0.0007 0.0013 0.002 0.0022 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.0928 34.3737 24.9523 31.7295 29.8044 29.2407 27.0109 27.282 26.7518 32.931
Translational Efficiency 1.5193 1.4025 1.5125 1.0652 1.4111 1.2816 1.0135 0.9253 0.9116 1.078

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
175 162 2459 1152 586 1889 172 771 761 2051 2631 1923

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 920.83 1035.43 1110.19 1105.27 933.47 974.35 1213.26 1185.28 930.56 979.17 1116.93 1137.35
Standard Deviation 150.31 143.28 119.05 168.00 118.85 127.72 146.11 156.95 126.89 130.06 123.65 168.29
Intensity Change Log 2 — 0.169223 0.269800 0.263392 — 0.061836 0.378213 0.344552 — 0.116163 0.325394 0.304819

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001493 0.000447 0.000607 0.001023 0.000402 0.000195 0.000327 0.000326 0.000653 0.000215 0.000589 0.000743
Bud Neck 0.006972 0.002224 0.001409 0.005539 0.002369 0.002821 0.002066 0.001948 0.003428 0.002773 0.001452 0.004099
Bud Site 0.092885 0.040830 0.014292 0.028380 0.025694 0.022824 0.018267 0.022151 0.041145 0.024246 0.014552 0.025882
Cell Periphery 0.000545 0.000232 0.000045 0.000277 0.000337 0.000150 0.000129 0.000110 0.000385 0.000156 0.000050 0.000210
Cytoplasm 0.460153* 0.641058* 0.833938* 0.608946* 0.653631* 0.728343* 0.487823* 0.671051* 0.609139* 0.721449* 0.811311* 0.633846*
Cytoplasmic Foci 0.266421* 0.220267* 0.038029 0.057932 0.219329* 0.146769 0.108837 0.069532 0.230158* 0.152574 0.042658 0.062583
Eisosomes 0.000144 0.000021 0.000006 0.000236 0.000042 0.000020 0.000043 0.000008 0.000066 0.000020 0.000009 0.000144
Endoplasmic Reticulum 0.007214 0.002038 0.000223 0.001538 0.004220 0.001099 0.000936 0.000759 0.004909 0.001173 0.000269 0.001226
Endosome 0.028428 0.022100 0.002364 0.012196 0.017395 0.010775 0.013754 0.012636 0.019932 0.011669 0.003108 0.012372
Golgi 0.011783 0.011377 0.000536 0.005101 0.008392 0.012308 0.000345 0.004189 0.009172 0.012234 0.000523 0.004736
Lipid Particles 0.008133 0.000944 0.000375 0.002103 0.001895 0.000929 0.001569 0.000899 0.003329 0.000930 0.000454 0.001620
Mitochondria 0.003244 0.002144 0.000103 0.004415 0.001019 0.001784 0.000037 0.001528 0.001530 0.001812 0.000099 0.003257
Mitotic Spindle 0.008937 0.002545 0.004493 0.009824 0.000839 0.004919 0.000603 0.011827 0.002701 0.004732 0.004239 0.010627
None 0.000378 0.000240 0.000837 0.001607 0.000491 0.000681 0.000363 0.000190 0.000465 0.000646 0.000806 0.001039
Nuclear Periphery 0.000176 0.000022 0.000058 0.000448 0.000053 0.000018 0.000221 0.000155 0.000081 0.000018 0.000068 0.000330
Nuclear Periphery Foci 0.003019 0.000588 0.000886 0.001353 0.000934 0.000167 0.007384 0.001229 0.001414 0.000201 0.001311 0.001303
Nucleolus 0.001317 0.000047 0.000127 0.000737 0.000606 0.000128 0.000122 0.000168 0.000769 0.000121 0.000126 0.000509
Nucleus 0.011189 0.002001 0.019767 0.033740 0.005636 0.005287 0.018102 0.005186 0.006913 0.005028 0.019658 0.022291
Peroxisomes 0.004265 0.001495 0.000815 0.002383 0.000978 0.001471 0.000754 0.001142 0.001734 0.001473 0.000811 0.001886
Vacuole 0.074396 0.044561 0.080825 0.218843* 0.047919 0.054461 0.337984* 0.189007 0.054007 0.053679 0.097636 0.206881*
Vacuole Periphery 0.008907 0.004818 0.000265 0.003380 0.007821 0.004853 0.000335 0.005959 0.008071 0.004851 0.000269 0.004414

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.77 2.25 1.37 -1.84 -1.22 3.28 1.11 1.64 -1.48 0.87 4.44 0.71 0.02 -3.14 -0.65
Bud Neck 3.71 4.50 1.54 -3.28 -4.72 -1.09 1.11 1.72 2.67 0.93 1.42 5.38 -0.31 -1.58 -4.86
Bud Site 3.98 6.48 5.36 3.18 -5.29 1.34 3.35 2.50 1.60 -0.41 4.80 8.21 5.03 0.75 -5.39
Cell Periphery 4.37 7.77 4.53 0.06 -6.40 4.37 6.18 6.87 1.98 2.00 5.92 11.26 6.10 -0.54 -6.92
Cytoplasm -6.19 -17.69 -7.21 1.12 20.42 -6.54 7.36 -0.74 5.52 -7.80 -10.38 -17.50 -2.16 10.18 19.61
Cytoplasmic Foci 2.18 15.46 14.12 10.52 -6.89 9.06 12.35 19.82 16.36 3.98 10.44 28.58 25.32 22.07 -7.93
Eisosomes 3.89 4.36 -0.53 -2.62 -2.86 6.03 0.41 9.74 9.24 9.85 5.74 7.20 -1.22 -2.50 -2.80
Endoplasmic Reticulum 2.41 3.54 2.98 0.88 -3.07 3.92 4.36 4.38 1.55 0.63 4.84 6.18 5.01 0.73 -3.08
Endosome 1.26 8.83 5.69 2.72 -6.26 4.24 3.81 3.58 0.40 0.03 5.66 12.93 6.07 1.39 -7.80
Golgi 0.14 7.41 4.15 2.26 -3.12 -4.12 15.01 5.18 7.88 -3.19 -3.26 15.80 6.02 8.10 -4.16
Lipid Particles 2.29 2.46 1.99 -3.48 -6.22 5.05 2.04 5.25 0.63 2.64 3.24 3.85 2.45 -3.59 -6.86
Mitochondria 0.53 3.11 -0.06 -0.55 -2.81 -1.41 2.50 -0.11 0.96 -1.77 -0.49 3.79 -1.10 -0.79 -3.21
Mitotic Spindle 1.13 0.88 0.12 -2.01 -2.00 -3.08 -0.33 -2.88 -1.31 -2.77 -1.17 -0.85 -2.88 -1.96 -2.56
None 1.10 -3.83 -1.77 -2.07 -0.92 -1.38 -0.22 2.53 5.58 2.49 -1.57 -3.29 -1.17 -0.62 -0.12
Nuclear Periphery 2.64 1.54 -1.25 -2.45 -1.99 2.82 -8.94 -1.27 -1.87 2.22 3.82 -1.10 -2.14 -2.89 -1.97
Nuclear Periphery Foci 2.45 2.05 1.66 -4.23 -2.29 6.98 -8.74 -1.84 -8.63 8.33 4.91 -0.11 0.38 -10.71 0.99
Nucleolus 1.43 1.33 0.79 -3.24 -2.71 1.15 1.18 1.07 -0.63 -0.92 1.69 1.65 0.89 -2.90 -2.74
Nucleus 2.68 -3.70 -4.03 -10.03 -1.65 0.66 -6.55 -0.36 -0.89 6.29 2.10 -15.48 -6.79 -8.84 2.57
Peroxisomes 2.50 3.22 1.93 -1.40 -3.30 -2.00 1.75 -0.01 2.33 -2.34 0.85 3.07 0.35 -0.56 -3.22
Vacuole 2.30 -2.74 -13.12 -17.40 -16.32 -1.50 -14.83 -17.50 -17.90 6.41 0.04 -13.00 -25.22 -28.42 -16.31
Vacuole Periphery 2.04 5.26 3.43 1.53 -3.11 1.48 3.80 1.46 0.36 -3.28 2.01 4.96 2.74 2.01 -4.53
Standard name
Human Ortholog
Description Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Rba50

Rba50


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rba50-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available