Standard name
Human Ortholog
Description Protein involved in regulation of cell wall composition and integrity; also involved in cell wall response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; HLR1 has a paralog, LRE1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0.13 0.11 0 0 0 0.05 0 0 0 0 0.09 0.06 0.05 0.08 0.07 0.11 0 0 0.06 0 0 0
Cytoplasm 0.96 0.99 0.99 0.97 0.97 0.92 0.86 0.86 0.82 0.74 0.65 0.73 1.0 0.99 0.99 0.98 0.98 0.98 0.76 0.9 0.75 0.9 0.88 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.1 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.05 0.17 0.3 0.24 0.33 0.43 0.52 0.45 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 1 0 0 0 0 0 0 0 2 0 0 1 2 0 0 8 1 8 3 5 7
Bud 0 3 1 1 2 3 14 11 10 17 17 8 1 0 0 0 0 0 3 0 5 1 7 9
Bud Neck 6 0 2 0 3 1 4 1 1 1 0 1 5 9 13 10 2 2 6 1 14 8 5 8
Bud Site 0 0 0 0 0 0 0 2 0 0 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 13 4 40 24 9 13 10 27 7 18 11 16 32 41 33 28 19 23 5 0 18 7 8 18
Cytoplasm 230 119 314 221 310 393 528 451 287 365 208 288 353 634 662 349 275 203 296 113 244 345 423 493
Endoplasmic Reticulum 0 0 0 0 0 1 3 0 2 10 2 5 0 4 4 15 17 13 38 0 7 1 3 1
Endosome 1 0 0 0 2 1 2 7 2 1 0 0 0 1 0 1 0 0 2 0 0 1 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 2 0 0 1
Mitochondria 0 3 4 14 16 74 183 126 115 210 166 178 0 0 1 3 7 6 10 3 15 5 8 8
Nucleus 1 0 0 1 0 0 2 1 1 1 2 3 2 3 1 3 2 4 0 0 0 1 2 2
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 0 0 1 0 7 9 2 3 4 8 0 0 0 1 2 1 2 0 1 0 2 3
Vac/Vac Membrane 0 0 0 2 1 0 1 2 4 3 0 0 0 1 0 1 1 0 5 0 1 0 3 3
Unique Cell Count 240 120 318 228 319 425 613 522 351 490 322 394 353 640 667 357 281 208 390 126 324 384 482 577
Labelled Cell Count 254 129 363 264 345 486 755 638 431 630 414 511 393 694 715 413 326 252 390 126 324 384 482 577


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.1 5.0 4.4 4.2 3.6 3.5 3.8 3.4 3.4 3.0 3.2 6.4 6.2 5.6 6.1 6.0 6.2 5.7 5.9 5.9
Std Deviation (1e-4) 0.7 1.2 1.3 1.3 0.9 1.3 1.0 1.6 1.0 1.0 1.0 1.2 1.0 1.1 0.9 1.2 1.5 1.6 1.0 1.3 1.2
Intensity Change (Log2) – – – -0.16 -0.23 -0.45 -0.52 -0.39 -0.53 -0.56 -0.72 -0.63 0.36 0.31 0.17 0.29 0.27 0.32 0.2 0.26 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 2.1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 -1.5 -3.2 -4.3 -2.0 -2.4 -0.5
Cytoplasm -1.5 -1.4 -3.9 -6.2 -6.1 -7.3 -9.2 -11.1 -9.4 2.1 0.5 0.8 -1.0 -0.8 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.7 4.4 4.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 1.1 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.642 2.484 2.7535 2.196 1.5006 2.2262 0.1279 1.452 1.2828 1.0101 -0.0299 0.5621 -0.5876 0.852 0.5111 0.2804 0.3258 -0.053
Actin 0.0274 0.0009 0.0062 0.0284 0.0004 0.0109 0.0302 0.0183 0.0022 0.0015 0.0001 0.0118 0.0085 0.0011 0.0098 0.0048 0.0042 0.0011
Bud 0.0014 0.0007 0.0024 0.0036 0.0002 0.0005 0.0008 0.0007 0.0008 0.0004 0 0.0002 0.0086 0.0143 0.0147 0.0153 0.0013 0.0132
Bud Neck 0.0084 0.0007 0.0009 0.0031 0.001 0.0061 0.0059 0.0007 0.0025 0.0013 0.0003 0.0021 0.0006 0.0005 0.0007 0.0007 0.0134 0.0042
Bud Periphery 0.0054 0.001 0.0043 0.0107 0.0005 0.0018 0.001 0.0015 0.0012 0.0006 0.0001 0.0004 0.0066 0.0038 0.0082 0.0072 0.0021 0.0074
Bud Site 0.01 0.0579 0.0195 0.0087 0.0004 0.0005 0.023 0.021 0.0078 0.003 0 0.0006 0.0072 0.0318 0.0271 0.007 0.0073 0.0006
Cell Periphery 0.0089 0.006 0.0047 0.0027 0.0015 0.0025 0.004 0.0029 0.0023 0.0017 0.0003 0.0011 0.0027 0.0033 0.0022 0.0016 0.0031 0.0007
Cytoplasm 0.3619 0.487 0.5606 0.5141 0.4923 0.5704 0.2441 0.6734 0.7795 0.6268 0.7262 0.745 0.4548 0.6472 0.68 0.5172 0.5963 0.6495
Cytoplasmic Foci 0.0075 0.0031 0.0069 0.0148 0.0023 0.0043 0.075 0.0046 0.017 0.0237 0.0035 0.0018 0.0322 0.0274 0.0172 0.0175 0.0193 0.0364
Eisosomes 0.0008 0.0002 0.0004 0.0006 0.0003 0.0002 0.0008 0.0002 0 0.0001 0 0.0004 0.0004 0.0002 0.0002 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0048 0.0012 0.0034 0.004 0.0011 0.002 0.0036 0.004 0.0035 0.0039 0.0019 0.0038 0.0055 0.0075 0.0048 0.0124 0.005 0.0021
Endosome 0.0041 0.0004 0.0028 0.0023 0.0002 0.0008 0.0308 0.0015 0.0069 0.0244 0.0028 0.0006 0.0129 0.0018 0.0023 0.0192 0.0252 0.0016
Golgi 0.0027 0.0002 0.0004 0.0012 0.0001 0.0006 0.0105 0.0015 0.0021 0.0097 0.0002 0.0007 0.0026 0.0008 0.0009 0.0032 0.0039 0.0002
Lipid Particles 0.0018 0.0004 0.0007 0.0011 0.0002 0.0005 0.0298 0.0007 0.0011 0.0294 0.0001 0.0008 0.0084 0.0024 0.0024 0.0112 0.0045 0.0006
Mitochondria 0.0007 0.0001 0.0002 0.0008 0.0001 0.0002 0.0021 0.0003 0.0003 0.0125 0 0.0071 0.0074 0.0017 0.0051 0.002 0.0016 0.0024
None 0.5437 0.4371 0.3777 0.3947 0.4974 0.3961 0.5027 0.2648 0.1582 0.2323 0.2629 0.2212 0.4104 0.2444 0.204 0.3668 0.2741 0.2701
Nuclear Periphery 0.0026 0.0002 0.0015 0.0011 0.0002 0.0003 0.0007 0.0005 0.0007 0.0004 0.0002 0.0002 0.0108 0.0007 0.0018 0.002 0.0115 0.0004
Nucleolus 0.0004 0.0004 0.0003 0.0003 0.0001 0.0001 0.0007 0.0001 0.0003 0.0016 0 0.0001 0.002 0.0041 0.0015 0.0008 0.0013 0.0012
Nucleus 0.0016 0.0008 0.0021 0.001 0.0004 0.0003 0.0006 0.0007 0.0015 0.0008 0.0002 0.0003 0.0032 0.0014 0.0028 0.0022 0.0043 0.0021
Peroxisomes 0.0013 0.0001 0.0005 0.0018 0.0001 0.0007 0.0247 0.0007 0.0044 0.018 0 0.001 0.0034 0.0003 0.0077 0.001 0.0009 0.0002
Punctate Nuclear 0.0014 0.0006 0.0027 0.0031 0.0004 0.0007 0.0056 0.0004 0.0013 0.0005 0.0001 0.0001 0.0065 0.0021 0.0041 0.0045 0.0121 0.0039
Vacuole 0.003 0.0014 0.0015 0.0014 0.0007 0.0006 0.0031 0.0015 0.0059 0.0063 0.0009 0.0005 0.004 0.0026 0.002 0.0027 0.0074 0.0015
Vacuole Periphery 0.0002 0 0.0001 0.0002 0 0.0001 0.0003 0.0001 0.0004 0.0011 0.0001 0.0001 0.0013 0.0004 0.0006 0.0006 0.0012 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.6142 12.4072 13.8101 30.6278 20.2445 15.6056 15.0931 20.3737 21.1317 30.8531
Translational Efficiency 0.672 0.6316 0.6865 0.3217 0.5285 1.3781 0.457 0.4517 0.4524 0.5164

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1304 661 119 1148 1311 1450 1788 117 2615 2111 1907 1265

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 732.28 736.80 1070.75 869.06 710.74 777.09 980.03 988.77 721.48 764.47 985.69 880.13
Standard Deviation 86.42 116.68 127.57 128.81 92.02 102.66 118.65 114.15 89.92 108.86 121.23 132.16
Intensity Change Log 2 — 0.008878 0.548154 0.247060 — 0.128760 0.463504 0.476313 — 0.069169 0.507081 0.364527

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000589 0.001633 0.001744 0.002081 0.000334 0.002504 0.000795 0.000738 0.000461 0.002231 0.000854 0.001956
Bud Neck 0.032131 0.030057 0.005011 0.004455 0.016582 0.021234 0.004408 0.009296 0.024336 0.023997 0.004445 0.004902
Bud Site 0.010820 0.030256 0.023251 0.047011 0.006983 0.017700 0.018526 0.033352 0.008896 0.021631 0.018820 0.045748
Cell Periphery 0.000581 0.000252 0.001317 0.000492 0.000366 0.000414 0.000414 0.000448 0.000473 0.000363 0.000470 0.000488
Cytoplasm 0.529760* 0.422816* 0.302629* 0.522407* 0.541394* 0.500914* 0.616948* 0.360911* 0.535593* 0.476460* 0.597334* 0.507470*
Cytoplasmic Foci 0.211994* 0.260358* 0.063545 0.056520 0.164701 0.190406* 0.046328 0.060895 0.188284* 0.212310* 0.047402 0.056925
Eisosomes 0.001113 0.000330 0.000253 0.000216 0.000416 0.000308 0.000135 0.000202 0.000764 0.000315 0.000143 0.000215
Endoplasmic Reticulum 0.001288 0.000920 0.010290 0.001438 0.001466 0.001046 0.001500 0.003072 0.001378 0.001007 0.002048 0.001589
Endosome 0.016066 0.038289 0.011722 0.004566 0.006379 0.016091 0.003268 0.004623 0.011210 0.023042 0.003795 0.004571
Golgi 0.004635 0.008840 0.012770 0.002277 0.001874 0.004227 0.000537 0.001409 0.003251 0.005671 0.001301 0.002196
Lipid Particles 0.008067 0.005550 0.006214 0.001897 0.005094 0.005383 0.001299 0.002191 0.006576 0.005435 0.001606 0.001924
Mitochondria 0.002928 0.004559 0.000224 0.000778 0.002856 0.003389 0.001404 0.000091 0.002892 0.003755 0.001330 0.000715
Mitotic Spindle 0.000375 0.004304 0.000472 0.007037 0.001100 0.006269 0.005709 0.018725 0.000739 0.005654 0.005383 0.008118
None 0.001019 0.001941 0.000592 0.000525 0.001406 0.001577 0.001252 0.002975 0.001213 0.001691 0.001211 0.000752
Nuclear Periphery 0.000171 0.000189 0.001180 0.000250 0.000264 0.000397 0.000297 0.000356 0.000218 0.000332 0.000352 0.000260
Nuclear Periphery Foci 0.000438 0.000712 0.014979 0.001994 0.000356 0.000704 0.002489 0.002632 0.000396 0.000706 0.003268 0.002053
Nucleolus 0.000310 0.001359 0.000187 0.000071 0.000389 0.000925 0.000081 0.000052 0.000350 0.001061 0.000088 0.000070
Nucleus 0.086535 0.046854 0.181235* 0.047479 0.187100* 0.110294 0.092970 0.054638 0.136952* 0.090429 0.098477 0.048141
Peroxisomes 0.005107 0.016868 0.000474 0.001187 0.003549 0.009010 0.000474 0.001999 0.004326 0.011471 0.000474 0.001262
Vacuole 0.084985 0.122098 0.361616* 0.295102* 0.056765 0.106030 0.200739* 0.441337* 0.070837 0.111061 0.210778* 0.308627*
Vacuole Periphery 0.001085 0.001815 0.000295 0.002217 0.000625 0.001178 0.000430 0.000059 0.000854 0.001378 0.000421 0.002017

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.86 -4.56 -3.68 -0.06 0.35 -2.26 -2.46 -2.34 2.05 1.25 -2.59 -2.16 -4.36 0.90 -2.61
Bud Neck 0.83 11.61 13.29 9.22 2.94 -2.29 10.44 7.06 8.18 0.38 0.41 15.90 16.63 14.18 1.65
Bud Site -6.57 -3.93 -6.89 -0.64 -2.39 -5.08 -6.06 -1.83 0.57 0.17 -7.32 -5.32 -7.84 -3.04 -5.04
Cell Periphery 4.56 -3.70 2.60 -4.14 5.45 -0.72 0.17 1.58 1.93 1.68 2.32 1.55 2.12 -0.54 1.07
Cytoplasm 8.44 12.55 6.05 -3.09 -9.16 4.47 -1.63 11.79 9.97 12.47 8.06 -0.40 9.59 2.81 9.05
Cytoplasmic Foci -4.94 19.71 30.11 25.49 0.41 -3.51 25.41 17.10 20.05 0.18 -4.06 39.30 35.60 35.22 -3.15
Eisosomes 4.21 4.80 5.11 2.97 2.25 2.11 5.88 5.09 5.55 -1.74 4.71 6.68 6.18 5.72 -4.42
Endoplasmic Reticulum 0.84 -7.25 -0.64 -2.37 7.25 1.43 -1.09 -2.69 -3.61 -2.35 1.48 -4.09 -1.15 -3.95 4.04
Endosome -7.44 4.48 12.46 12.51 5.18 -7.65 7.77 6.53 10.79 0.43 -8.81 14.20 12.53 15.69 -0.04
Golgi -2.20 -0.29 5.02 4.07 0.83 -3.08 4.29 3.52 5.00 -0.08 -3.00 5.04 3.75 5.00 -0.63
Lipid Particles 3.20 2.85 11.38 5.54 6.44 -0.48 8.24 6.30 7.87 -2.22 2.47 14.14 12.13 9.51 -0.61
Mitochondria -1.43 3.74 3.44 5.02 -0.66 -0.49 2.33 4.33 4.32 1.56 -1.05 2.94 4.89 5.40 0.81
Mitotic Spindle -2.58 -0.93 -3.26 -0.32 -2.58 -3.41 -2.87 -1.21 -0.16 -0.46 -4.34 -3.26 -3.27 0.25 -0.42
None -2.31 1.18 3.14 4.13 0.96 -0.37 0.86 0.46 0.62 0.15 -1.52 0.34 4.37 4.15 3.95
Nuclear Periphery -1.03 -8.95 -4.03 -2.90 7.95 -1.87 -7.71 -3.72 -0.90 -1.24 -2.22 -11.98 -3.90 0.66 5.86
Nuclear Periphery Foci -2.66 -5.70 -6.41 -5.24 4.87 -3.86 -12.79 -4.35 -3.60 -0.84 -4.19 -12.48 -7.56 -5.91 2.50
Nucleolus -2.39 2.99 7.84 2.93 2.53 -2.94 6.92 7.81 4.79 5.41 -3.85 10.15 9.47 5.31 0.73
Nucleus 8.04 -5.29 8.86 -0.63 6.89 11.18 12.06 14.45 7.14 7.01 10.17 4.59 21.16 11.21 15.78
Peroxisomes -5.33 7.58 6.54 7.60 -2.39 -5.41 6.54 4.98 8.79 -0.96 -7.26 10.03 8.05 11.56 -2.79
Vacuole -6.11 -12.79 -32.40 -24.23 0.50 -11.36 -32.10 -20.91 -18.71 -13.33 -11.42 -32.51 -38.62 -31.60 -13.63
Vacuole Periphery -2.24 3.82 -0.16 0.83 -1.49 -1.48 1.44 3.00 3.69 1.58 -1.87 2.91 -0.38 0.41 -1.35
Standard name
Human Ortholog
Description Protein involved in regulation of cell wall composition and integrity; also involved in cell wall response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; HLR1 has a paralog, LRE1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (63%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hlr1

Hlr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hlr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available