Standard name
Human Ortholog
Description Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Spc105p; modified by sumoylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.05 0.07 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.06 0 0 0 0.06 0.06 0.06 0.06 0.08 0.07 0.11 0.09 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0.05 0.07 0.05 0 0 0.05 0 0 0.08 0.13 0.18 0.18 0.12 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.08 0.09 0.08 0 0 0.06 0 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0.09 0 0.07 0.05 0.08 0.1 0.09 0.1 0.15 0.13 0.13 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Nucleus 0.05 0 0 0.05 0 0.05 0 0.06 0 0.05 0.06 0.05 0 0 0.05 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.54 0.72 0.36 0.28 0.3 0.37 0.38 0.36 0.44 0.46 0.49 0.46 0.52 0.51 0.45 0.44 0.48 0.5 0 0 0 0.05 0.06 0
Peroxisomes 0 0 0.06 0.06 0 0.06 0.05 0.05 0 0 0.07 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0.73 0.63 0.82 0.77 0.84 0.72 0.72 0.72 0.75 0.65 0.73 0.72 0.8 0.77 0.71 0.67 0.74 0.71 0.9 0.88 0.89 0.83 0.77 0.74
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Bud 3 0 5 7 4 4 13 18 12 11 11 13 0 0 0 3 0 1 2 1 1 0 5 11
Bud Neck 8 3 3 5 7 6 6 16 11 5 11 19 6 5 5 2 1 3 1 1 1 1 4 7
Bud Site 10 0 3 10 15 16 21 25 27 21 28 29 6 1 2 5 4 4 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 5 2 11 20 12 18 20 12 6 14 4 7 26 51 79 21 15 10 0 0 1 1 5 4
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 1 2 1 0 0 0 0 0 0
Endosome 0 0 18 9 2 4 7 16 3 2 2 1 14 26 36 9 11 10 2 1 14 9 27 27
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 2 0
Mitochondria 32 4 1 16 12 22 35 39 32 46 34 44 0 1 0 9 7 2 0 0 2 1 6 6
Nucleus 8 2 6 11 10 14 16 24 14 16 15 16 14 12 22 5 4 6 1 1 0 6 6 13
Nuclear Periphery 0 0 0 0 0 0 0 1 1 1 0 0 1 3 1 0 1 0 0 0 0 0 0 2
Nucleolus 85 33 91 65 73 99 137 150 140 141 130 155 177 193 198 51 60 60 4 1 2 12 24 19
Peroxisomes 5 0 15 14 11 17 20 20 11 8 18 22 2 4 9 3 1 6 0 0 1 2 5 5
SpindlePole 115 29 210 179 206 194 262 297 238 197 194 244 273 293 314 78 92 86 143 74 233 206 331 326
Vac/Vac Membrane 0 1 1 1 1 0 1 1 0 0 0 0 4 1 1 5 1 2 0 0 0 0 4 8
Unique Cell Count 157 46 255 233 245 270 365 412 318 304 265 338 341 382 444 117 124 121 159 85 263 248 433 440
Labelled Cell Count 271 74 365 340 353 394 538 619 495 462 447 550 523 591 668 193 199 192 159 85 263 248 433 440


Ambiguous, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.8 5.2 4.8 5.2 4.6 4.5 4.3 4.4 4.2 4.1 4.1 6.4 6.5 6.3 7.2 6.6 6.9 6.2 6.5 6.8
Std Deviation (1e-4) 0.5 0.9 1.7 1.6 2.2 1.7 1.7 1.5 1.6 1.5 1.5 1.3 1.3 1.5 1.2 2.1 1.5 1.9 1.1 1.4 1.6
Intensity Change (Log2) – – – -0.12 0.0 -0.18 -0.22 -0.26 -0.22 -0.29 -0.33 -0.35 0.3 0.32 0.27 0.48 0.35 0.41 0.25 0.32 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 3.0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 0.3 1.2 0.7 -1.0 -1.7 0.2 -1.9 -1.6 1.7 3.8 5.1 4.4 2.8 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 -3.6 0 0 0 0 0 0 0 -1.6 -0.1 0.5 0.2 0.6 0.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 1.4 1.1 1.7 1.3 2.1 1.3 1.8 1.9 1.5 1.2 0.6 1.7 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 -1.4 0 0 0 0 0 0 0 3.9 3.7 2.3 1.5 2.4 2.6
Peroxisomes 0 -0.7 0 0 0 0 0 0 0 -3.8 -3.5 -2.7 -1.4 -2.3 -0.4
SpindlePole 0 0.5 0 0 0 0 0 0 0 -0.7 -1.7 -3.4 -3.4 -1.9 -2.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1965 0.2834 0.1321 0.2742 -0.0001 0.3152 1.1758 0.5447 0.7913 0.6731 0.9405 0.6083 0.619 0.4374 0.385 0.7213 0.5815 0.4816
Actin 0.0204 0 0.0004 0 0.0002 0 0.0339 0 0.0054 0.0009 0.0314 0.0044 0.0091 0 0.0056 0.0052 0.001 0.0014
Bud 0.0001 0.0001 0 0 0 0 0.0009 0 0.0001 0.0004 0.0004 0 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001
Bud Neck 0.0009 0.0004 0.0002 0.0004 0.0006 0.0002 0.0019 0.0008 0.0012 0.0007 0.0005 0.0003 0.0005 0.0005 0.0006 0.0007 0.0003 0.0003
Bud Periphery 0.0001 0 0 0 0 0 0.0013 0 0.0002 0.0004 0.0006 0.0001 0.0003 0.0001 0.0003 0.0002 0.0003 0.0001
Bud Site 0.0006 0.0004 0.0001 0.0001 0.0002 0 0.0038 0.0006 0.0013 0.0042 0.001 0.0001 0.002 0.0045 0.0011 0.0028 0.0004 0.0003
Cell Periphery 0.0001 0 0 0 0 0 0.0002 0 0 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.0013 0.0001 0.0003 0.0002 0.0001 0 0.0103 0.0003 0.0028 0.0004 0.0013 0 0.0005 0.0001 0.0003 0.0002 0.0009 0
Cytoplasmic Foci 0.0196 0.0062 0.0177 0.0174 0.0555 0.0032 0.0472 0.0066 0.0156 0.0188 0.0519 0.0038 0.0284 0.0056 0.0066 0.0215 0.0226 0.0038
Eisosomes 0.0008 0 0 0 0 0 0.0002 0 0.0001 0 0.0001 0 0.0004 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0015 0 0.0001 0 0 0 0.0061 0 0.0005 0.0002 0.0009 0.0005 0.0004 0 0.0004 0.0002 0.0021 0
Endosome 0.0068 0.0001 0.0018 0.0001 0.0023 0.0001 0.0556 0.0024 0.0099 0.0426 0.0194 0.0016 0.01 0.0001 0.003 0.0058 0.019 0.0017
Golgi 0.0023 0.0003 0.0004 0.0002 0.0019 0.0001 0.0229 0.0002 0.0028 0.0161 0.0283 0.0024 0.005 0.0001 0.0021 0.005 0.0026 0.0023
Lipid Particles 0.0115 0.0053 0.0073 0.0014 0.0263 0.0014 0.0374 0.0016 0.0082 0.028 0.0926 0.0058 0.0259 0.0008 0.0053 0.0292 0.0135 0.0148
Mitochondria 0.0004 0 0.0002 0 0 0 0.0092 0 0.0018 0.004 0.0121 0.0012 0.0073 0 0.0062 0.0022 0.0042 0.002
None 0.0127 0.0001 0.0007 0.0001 0.0001 0.0001 0.0046 0.0001 0.0011 0.0002 0.0005 0.0001 0.0008 0.0001 0.0002 0.0005 0.0005 0.0001
Nuclear Periphery 0.0084 0.0002 0.0013 0.0009 0.0006 0.0001 0.0229 0.0004 0.0013 0.0011 0.0079 0.007 0.0008 0.0001 0.0008 0.0016 0.0047 0.0004
Nucleolus 0.0214 0.0468 0.0328 0.0107 0.0044 0.015 0.0046 0.0883 0.0869 0.0365 0.0035 0.0164 0.0226 0.0468 0.0204 0.0014 0.0032 0.0061
Nucleus 0.0047 0.0156 0.0033 0.0057 0.0005 0.0013 0.0114 0.0649 0.0399 0.0035 0.0011 0.0035 0.0029 0.0155 0.0073 0.0005 0.0019 0.0013
Peroxisomes 0.0046 0.0008 0.0013 0.0006 0.0075 0.0001 0.0172 0.0003 0.0026 0.0123 0.0295 0.0003 0.007 0.0004 0.0035 0.013 0.0158 0.0067
Punctate Nuclear 0.8813 0.9234 0.9318 0.9622 0.8997 0.9784 0.6996 0.8333 0.8172 0.8268 0.7159 0.9518 0.8744 0.9253 0.9358 0.9094 0.8994 0.9584
Vacuole 0.0003 0 0.0001 0 0 0 0.0058 0.0001 0.0007 0.002 0.0005 0.0001 0.0011 0 0.0003 0.0002 0.0046 0.0001
Vacuole Periphery 0.0003 0 0.0001 0 0 0 0.0029 0 0.0003 0.0009 0.0005 0.0005 0.0004 0 0.0001 0.0002 0.0029 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.335 3.4072 3.7924 6.8912 12.3542 4.7616 8.1818 8.4286 6.2202 9.3212
Translational Efficiency 2.7773 1.8154 1.2495 0.7599 0.6547 1.8387 0.9119 0.6151 1.1078 1.149

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Spc105p; modified by sumoylation
Localization
Cell Percentages spindle pole (75%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kre28

Kre28


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kre28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available