Standard name
Human Ortholog
Description Phenylacrylic acid decarboxylase; decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; confers resistance to cinnamic acid; overexpression of both Pad1p and Fdc1p increases cinnamic acid decarboxylase activity due to the Pad1p-catalyzed formation of a diffusible cofactor required for Fdc1p activity; contains mRNA binding activity; homolog of E. coli UbiX

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0.07 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.12 0 0 0 0.08 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.38 0.38 0.48 0.54 0.51 0.39 0.46 0.46 0.31 0.35 0.35 0.4 0.61 0.62 0.71 0.15 0.1 0.25 0.09 0.13 0.22 0.2 0.19
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0.07 0 0 0 0 0 0 0.07 0.09 0.09 0.24 0.32 0.09 0.13 0.11 0.09 0.06 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.62 0.62 0.06 0.25 0.16 0.38 0.34 0.31 0.68 0.51 0.61 0.53 0.12 0 0 0.1 0.1 0.5 0.51 0.46 0.42 0.45 0.41
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.32 0.31 0 0 0.08 0 0 0.05
SpindlePole 0 0 0.33 0.15 0.2 0.16 0.2 0.19 0.1 0.2 0.13 0.17 0.14 0.21 0.16 0.26 0.33 0.05 0 0.12 0.07 0.08 0.09
Vac/Vac Membrane 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 11 2 4 0 1 0 0 0 0 0 12 14 2 0 0 1 5 3 7 14 21
Bud 0 2 5 12 5 17 9 19 25 24 26 22 8 12 3 3 6 0 1 0 1 15 8
Bud Neck 0 0 14 6 8 19 29 34 9 19 6 10 12 13 13 0 1 0 2 1 2 11 8
Bud Site 0 0 0 0 0 5 3 6 5 6 4 8 0 0 1 0 3
Cell Periphery 0 0 1 3 1 3 1 1 0 1 0 0 2 1 7 7 5 0 0 0 0 2 2
Cytoplasm 6 29 58 120 101 167 168 244 105 126 131 197 203 311 346 17 13 18 9 20 45 108 99
Endoplasmic Reticulum 1 3 2 1 2 0 1 1 0 0 0 1 0 6 0 9 6 0 0 0 3 2 3
Endosome 0 2 4 9 13 29 12 14 0 3 2 6 24 44 43 28 43 6 12 17 18 34 51
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 3 5 0 0 0 2 2 5
Mitochondria 10 48 7 55 32 161 123 163 227 183 230 258 41 15 4 11 13 36 49 76 86 239 217
Nucleus 0 0 1 0 2 9 6 11 5 10 9 10 0 1 1 1 6 0 0 0 0 3 2
Nuclear Periphery 1 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 0 0 0 1 0 4 1 1 3 2 1 0 0 1 4 0 0 0 0 1 3
Peroxisomes 0 0 1 9 5 11 1 2 6 1 2 11 10 26 19 37 41 1 3 13 5 12 25
SpindlePole 0 3 39 33 39 67 72 101 33 71 47 82 48 104 80 30 44 3 3 19 15 43 48
Vac/Vac Membrane 0 3 4 6 11 32 3 22 2 3 5 6 9 5 10 9 10 2 6 0 7 22 16
Unique Cell Count 16 77 120 222 200 425 365 525 336 359 374 489 332 503 486 115 134 72 97 165 204 531 529
Labelled Cell Count 19 92 147 256 223 521 429 623 418 449 465 614 371 554 530 156 200 72 97 165 204 531 529


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.9 4.6 4.6 5.4 4.2 4.2 4.5 3.6 3.9 3.6 3.6 4.8 5.1 5.0 4.1 5.6 5.8 5.2 5.5 5.7
Std Deviation (1e-4) 0.3 0.6 0.8 1.4 1.7 1.4 1.2 1.3 1.4 1.4 1.2 1.0 0.9 1.0 1.2 0.7 1.8 1.6 1.2 1.3 1.9
Intensity Change (Log2) -0.0 0.23 -0.12 -0.13 -0.05 -0.36 -0.25 -0.37 -0.34 0.07 0.15 0.11 -0.16 0.29 0.34 0.18 0.25 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 1.5 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.902 5.1493 4.9994 4.9668 4.722 4.9655 3.5338 4.1928 3.5131 3.1197 2.7962 3.7375 0.1808 0.7593 0.6183 0.7719 0.1365 0.4263
Actin 0.0302 0.0588 0.0343 0.0045 0.0477 0.0201 0.0304 0.0091 0.0223 0.0229 0.0121 0.016 0.0153 0.0163 0.0244 0.0068 0.0191 0.0091
Bud 0.0005 0.0011 0.0007 0.0012 0.0002 0.0003 0.0007 0.0011 0.0012 0.0014 0.0011 0.0004 0.0005 0.0022 0.0009 0.0005 0.0018 0.0003
Bud Neck 0.0008 0.0006 0.0074 0.001 0.0005 0.011 0.002 0.0016 0.0085 0.0008 0.0007 0.0011 0.0007 0.0013 0.0093 0.0013 0.0008 0.0013
Bud Periphery 0.0021 0.0044 0.0017 0.0025 0.0003 0.0005 0.0016 0.0034 0.0042 0.0028 0.0015 0.0011 0.0008 0.0052 0.0012 0.0007 0.0016 0.0017
Bud Site 0.0029 0.0032 0.0095 0.0091 0.0004 0.001 0.0021 0.0105 0.0097 0.0092 0.0043 0.0008 0.0019 0.0192 0.0093 0.0009 0.0025 0.0005
Cell Periphery 0.0018 0.0016 0.001 0.0003 0.0001 0.0001 0.0007 0.002 0.0008 0.0004 0.0002 0.0005 0.0004 0.0005 0.0004 0.0001 0.0004 0.0023
Cytoplasm 0.004 0.0063 0.0024 0.0033 0.0008 0.0016 0.0044 0.0048 0.0068 0.0057 0.0034 0.0027 0.0047 0.0017 0.0021 0.0011 0.005 0.0024
Cytoplasmic Foci 0.0503 0.0301 0.0294 0.0535 0.0291 0.041 0.0648 0.0818 0.0487 0.0374 0.0515 0.036 0.0615 0.0388 0.0349 0.0446 0.0621 0.0329
Eisosomes 0.0021 0.0013 0.0025 0.0002 0.0002 0.0004 0.0008 0.0011 0.0005 0.0006 0.0002 0.0004 0.0003 0.0006 0.0005 0.0002 0.0003 0.0004
Endoplasmic Reticulum 0.0027 0.0018 0.0003 0.0001 0 0.0001 0.0023 0.0008 0.0009 0.0003 0.0021 0.0008 0.0044 0.0012 0.0004 0.0003 0.0006 0.0015
Endosome 0.0378 0.0428 0.0226 0.0316 0.0072 0.0222 0.1293 0.0844 0.0498 0.0413 0.098 0.0528 0.0856 0.0601 0.0495 0.0827 0.0565 0.067
Golgi 0.0435 0.0569 0.0401 0.0396 0.0156 0.0358 0.0879 0.0837 0.0537 0.0618 0.0802 0.0615 0.0664 0.0867 0.0618 0.0521 0.0901 0.0699
Lipid Particles 0.0501 0.0146 0.0141 0.0141 0.0086 0.0241 0.0619 0.034 0.0261 0.007 0.0187 0.033 0.0933 0.0258 0.0322 0.0106 0.0313 0.0455
Mitochondria 0.5825 0.6533 0.6604 0.6177 0.7709 0.6579 0.4407 0.5204 0.6573 0.6426 0.6575 0.6708 0.5158 0.6536 0.6648 0.7214 0.5748 0.6799
None 0.0238 0.0336 0.0174 0.0297 0.0003 0.0167 0.0319 0.0265 0.0032 0.0067 0.0008 0.0062 0.018 0.0113 0.0009 0.0029 0.0044 0.0031
Nuclear Periphery 0.0015 0.0002 0.0002 0 0 0.0001 0.0018 0.0002 0.0019 0.0001 0.0023 0.0005 0.0045 0.0004 0.0002 0.0002 0.001 0.0045
Nucleolus 0.0005 0.0003 0.0005 0.0002 0 0.0002 0.0007 0.0007 0.0015 0.0002 0.0004 0.0003 0.0008 0.0005 0.0006 0.0002 0.0008 0.0003
Nucleus 0.0003 0.0002 0.0003 0.0002 0 0.0001 0.0009 0.0005 0.0008 0.0002 0.0007 0.0002 0.005 0.0003 0.0004 0.0001 0.0005 0.0004
Peroxisomes 0.1576 0.0827 0.1513 0.1888 0.1174 0.1649 0.1214 0.1263 0.0847 0.1548 0.0512 0.1092 0.1063 0.0675 0.0955 0.0651 0.1373 0.0701
Punctate Nuclear 0.0004 0.0001 0.0004 0.0004 0.0001 0.0004 0.0021 0.0004 0.0004 0.0003 0.0043 0.0017 0.0015 0.0008 0.0005 0.0003 0.0045 0.0005
Vacuole 0.0025 0.0047 0.0021 0.001 0.0002 0.0006 0.0071 0.0041 0.0122 0.0022 0.0042 0.0018 0.0072 0.0038 0.0056 0.0044 0.0023 0.0028
Vacuole Periphery 0.002 0.0014 0.0012 0.0011 0.0003 0.0007 0.0045 0.0026 0.0049 0.0013 0.0048 0.0021 0.0053 0.0022 0.0046 0.0034 0.0024 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.2989 20.2958 19.0765 17.8137 22.568 39.3315 26.3354 22.1605 20.4843 29.3715
Translational Efficiency 0.7191 0.9229 0.7151 0.7713 0.8656 0.6295 0.6776 0.7294 0.7395 0.5772

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1535 822 1507 1243 1606 1765 1903 879 3141 2587 3410 2122

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 625.93 679.78 742.68 785.92 676.96 795.97 836.73 770.60 652.02 759.05 795.17 779.57
Standard Deviation 87.89 114.38 90.71 111.49 78.64 91.34 102.23 95.87 87.11 113.03 107.94 105.57
Intensity Change Log 2 0.119067 0.246739 0.328381 0.233643 0.305692 0.186912 0.179733 0.277670 0.256609

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001195 0.001965 0.003437 0.006313 0.000856 0.000412 0.002753 0.001855 0.001022 0.000905 0.003055 0.004466
Bud Neck 0.033084 0.034391 0.073225 0.026441 0.017799 0.053848 0.040150 0.028165 0.025269 0.047666 0.054767 0.027155
Bud Site 0.017154 0.043819 0.080661 0.048836 0.017628 0.013549 0.031457 0.048369 0.017396 0.023167 0.053202 0.048643
Cell Periphery 0.000505 0.000279 0.000313 0.000329 0.000278 0.000233 0.000205 0.000321 0.000389 0.000248 0.000253 0.000326
Cytoplasm 0.035018 0.063884 0.135023* 0.278840* 0.037409 0.079893 0.257591* 0.341284* 0.036241 0.074806 0.203424* 0.304706*
Cytoplasmic Foci 0.155884 0.200089* 0.095842 0.103086 0.204744* 0.223977* 0.093460 0.105833 0.180866* 0.216387* 0.094513 0.104224
Eisosomes 0.000254 0.000149 0.000121 0.000146 0.000142 0.000179 0.000106 0.000146 0.000197 0.000170 0.000113 0.000146
Endoplasmic Reticulum 0.000567 0.000503 0.002548 0.003300 0.000625 0.000524 0.003923 0.001859 0.000597 0.000517 0.003315 0.002703
Endosome 0.075441 0.149244 0.072425 0.019582 0.092171 0.134848 0.040992 0.012319 0.083995 0.139422 0.054884 0.016574
Golgi 0.132832* 0.105916 0.021020 0.011088 0.155538* 0.074787 0.007696 0.006988 0.144442* 0.084678 0.013584 0.009390
Lipid Particles 0.020856 0.007536 0.005119 0.003173 0.012676 0.021610 0.008063 0.004838 0.016674 0.017138 0.006762 0.003862
Mitochondria 0.406270* 0.187327* 0.106551* 0.019742 0.329309* 0.196916* 0.022752 0.018756 0.366920* 0.193869* 0.059786 0.019334
Mitotic Spindle 0.008844 0.025319 0.090197 0.049426 0.023814 0.019738 0.073454 0.019823 0.016498 0.021512 0.080853 0.037164
None 0.003046 0.001989 0.001574 0.002552 0.001833 0.003574 0.005766 0.005371 0.002426 0.003070 0.003913 0.003720
Nuclear Periphery 0.000354 0.000162 0.001113 0.001259 0.000114 0.000469 0.002663 0.001235 0.000231 0.000372 0.001978 0.001249
Nuclear Periphery Foci 0.000348 0.000333 0.000718 0.001124 0.000308 0.000364 0.002299 0.000907 0.000327 0.000354 0.001600 0.001034
Nucleolus 0.001911 0.003523 0.003832 0.001033 0.001870 0.004906 0.006089 0.002244 0.001890 0.004466 0.005092 0.001535
Nucleus 0.006412 0.016193 0.100536 0.160300* 0.006231 0.030108 0.232917* 0.245912* 0.006319 0.025687 0.174413* 0.195763*
Peroxisomes 0.085102 0.106161* 0.097979 0.069913 0.073553 0.081600 0.047971 0.051028 0.079197 0.089404 0.070071 0.062090
Vacuole 0.008375 0.040419 0.102427 0.186549 0.013788 0.049489 0.115848 0.101549 0.011142 0.046607 0.109917 0.151339
Vacuole Periphery 0.006549 0.010798 0.005337 0.006966 0.009315 0.008978 0.003847 0.001197 0.007964 0.009556 0.004505 0.004576

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.53 -3.96 -4.25 -2.87 -1.09 4.28 -4.07 -3.67 -6.45 2.13 0.61 -5.53 -5.03 -5.32 -0.58
Bud Neck -0.25 -8.92 3.72 3.64 13.52 -10.86 -8.01 -2.27 7.77 4.46 -8.15 -11.36 1.36 9.49 13.03
Bud Site -7.16 -15.38 -5.62 2.18 9.26 2.41 -4.69 -5.06 -6.21 -2.45 -3.41 -14.91 -7.56 -4.90 4.42
Cell Periphery 2.39 2.12 2.81 1.02 1.09 1.38 2.85 0.31 -1.09 -2.67 2.91 3.02 2.74 -0.00 -0.39
Cytoplasm -5.42 -18.22 -29.57 -22.75 -13.37 -9.08 -32.60 -29.27 -23.81 -6.10 -10.87 -36.44 -41.34 -32.34 -11.59
Cytoplasmic Foci -6.02 11.65 14.01 16.86 2.97 -2.90 21.07 16.78 19.56 -0.61 -7.26 23.35 22.53 27.81 1.66
Eisosomes 4.22 5.53 4.90 1.42 -1.57 -3.15 4.76 0.07 3.09 -4.27 1.95 6.77 4.58 3.69 -4.23
Endoplasmic Reticulum 0.61 -8.66 -8.68 -9.00 -1.58 1.07 -17.28 -10.66 -12.26 8.37 1.13 -18.31 -11.48 -12.07 2.45
Endosome -10.26 2.05 18.16 21.05 15.09 -7.77 14.82 23.23 28.38 11.44 -12.94 11.86 29.22 35.21 18.59
Golgi 3.99 23.85 26.55 17.68 7.15 14.39 31.41 31.25 21.40 1.08 14.10 39.35 40.73 27.39 5.77
Lipid Particles 6.34 7.97 9.39 4.13 4.21 -4.92 4.15 7.03 11.09 5.50 -0.37 8.84 11.84 12.20 7.73
Mitochondria 17.37 30.68 43.20* 18.65 16.18* 12.95 36.97* 36.45* 27.15 1.75 21.94* 47.92* 56.22* 33.70 14.05
Mitotic Spindle -5.49 -16.51 -6.98 -2.08 8.64 1.54 -8.83 1.79 0.56 9.42 -2.64 -17.59 -4.36 -2.29 11.67
None 1.29 1.90 1.03 -0.56 -1.79 -2.10 -3.07 -3.00 -0.87 0.43 -0.97 -1.56 -1.33 -0.16 0.50
Nuclear Periphery 1.19 -5.58 -8.40 -15.94 -3.57 -3.80 -18.38 -8.14 -5.46 6.01 -1.44 -16.39 -12.60 -12.12 3.59
Nuclear Periphery Foci -0.08 -4.40 -8.88 -10.30 -4.30 -1.07 -7.92 -6.56 -5.93 5.22 -0.69 -8.74 -11.46 -11.54 3.60
Nucleolus -1.49 -3.72 1.97 2.19 6.00 -3.89 -5.13 -0.26 3.95 5.34 -4.18 -6.40 1.06 5.12 8.31
Nucleus -3.80 -20.18 -28.68 -25.23 -8.99 -10.44 -38.77 -29.10 -25.80 -1.59 -10.99 -41.93 -40.42 -35.24 -4.69
Peroxisomes -2.96 -0.89 8.20 9.21 8.60 -1.75 7.75 6.85 8.52 0.75 -2.66 4.71 10.54 12.40 6.33
Vacuole -9.69 -25.67 -34.76 -28.30 -18.24 -14.10 -29.66 -20.31 -14.43 -1.57 -17.73 -39.22 -38.96 -30.87 -15.26
Vacuole Periphery -3.32 2.64 2.76 4.90 1.21 0.47 7.52 11.73 15.36 4.10 -2.30 7.66 7.78 9.58 2.10
Standard name
Human Ortholog
Description Phenylacrylic acid decarboxylase; decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; confers resistance to cinnamic acid; overexpression of both Pad1p and Fdc1p increases cinnamic acid decarboxylase activity due to the Pad1p-catalyzed formation of a diffusible cofactor required for Fdc1p activity; contains mRNA binding activity; homolog of E. coli UbiX
Localization
Cell Percentages mitochondrion (75%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Pad1

Pad1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pad1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available