Standard name
Human Ortholog
Description Protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.24 0.22 0.32 0.1 0.07 0.08 0.09 – 0.09 0.06 0.07 0.07 0.34 0.32 0.33 0.62 0.69 0.67 0.12 0.14 0.14 0.18 0.11 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.13 0 0 0 0.32 0 – 0.39 0.26 0.43 0.37 0 0 0 0.06 0 0.1 0 0 0.05 0 0 0
Nucleus 0.44 0.56 0.59 0.81 0.9 0.78 0.82 – 0.73 0.81 0.71 0.74 0.46 0.49 0.5 0.2 0.14 0.16 0.5 0.54 0.52 0.38 0.34 0.28
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06
Vac/Vac Membrane 0.36 0.25 0.1 0.05 0.05 0 0.05 – 0 0.07 0 0 0.21 0.25 0.26 0.15 0.19 0.14 0.23 0.18 0.2 0.26 0.31 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 6 0 – 5 2 2 2 0 0 0 1 0 2 6 9 2 9 11 14
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 3 0 0 1 0 2 4 5
Bud Site 0 0 0 0 0 5 1 – 5 5 12 17 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 65 72 46 21 15 24 36 – 31 19 26 26 85 114 133 178 190 189 39 53 22 46 43 42
Endoplasmic Reticulum 1 3 0 0 0 0 1 – 0 0 0 1 2 1 0 14 20 16 0 1 0 1 1 2
Endosome 1 0 1 4 1 2 4 – 1 0 5 1 4 10 7 7 9 8 5 4 1 4 14 15
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 0 1 2 0 0 2 1 0 0 0 1
Mitochondria 18 44 2 8 1 93 13 – 132 78 170 142 6 6 3 18 6 27 11 11 7 7 17 14
Nucleus 120 185 84 169 181 230 333 – 246 246 278 286 116 174 201 58 38 45 161 203 84 100 130 110
Nuclear Periphery 0 0 0 1 0 1 4 – 2 1 2 4 1 0 1 0 0 0 1 0 0 0 2 7
Nucleolus 0 0 0 0 1 8 3 – 4 7 3 10 0 0 1 0 0 0 1 1 1 3 16 14
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 7 – 1 2 3 3 0 1 2 0 2 1 11 15 6 12 16 25
Vac/Vac Membrane 99 84 14 11 10 11 21 – 14 20 15 17 52 88 105 44 52 40 74 67 32 68 118 133
Unique Cell Count 274 333 142 208 202 293 405 337 305 394 385 250 353 405 286 277 284 321 377 163 264 386 402
Labelled Cell Count 305 388 147 215 210 380 424 441 380 516 510 267 395 455 323 320 328 321 377 163 264 386 402


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.6 5.4 5.5 7.0 4.6 6.0 – 4.9 5.0 4.6 4.9 5.1 5.5 5.7 5.6 5.5 4.9 4.7 5.0 5.4
Std Deviation (1e-4) 0.6 1.0 1.1 1.2 1.6 1.5 1.8 – 1.6 1.3 1.2 1.1 1.2 1.3 1.6 2.1 1.6 1.5 1.1 1.6 1.4
Intensity Change (Log2) – – – 0.01 0.36 -0.23 0.14 – -0.14 -0.13 -0.23 -0.16 -0.09 0.01 0.06 0.05 0.02 -0.14 -0.22 -0.12 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.6 2.4 1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0267 -0.9122 -0.8611 -1.2485 -0.8089 -0.8911 -0.0565 0.7385 0.4261 0.2542 0.3309 0.5255 3.1155 2.5554 2.1548 1.4632 2.8973 2.3277
Actin 0.0284 0.0009 0.0174 0.0001 0.0313 0.0007 0.0016 0.0011 0.0018 0.0005 0.0058 0.0008 0.0715 0.0039 0.0042 0.0007 0.0937 0.0083
Bud 0.0006 0.0034 0.0012 0 0.0002 0.0001 0.0001 0.0001 0.0005 0.0002 0.0004 0.0003 0.0018 0.0003 0.0026 0.0003 0.0088 0.002
Bud Neck 0.0073 0.0007 0.0022 0.0001 0.0019 0.0095 0.0004 0.0005 0.0017 0.0018 0.0012 0.0023 0.0028 0.0004 0.0011 0.0002 0.0018 0.0031
Bud Periphery 0.002 0.0007 0.0016 0 0.0003 0.0001 0.0001 0.0001 0.0008 0.0002 0.0008 0.0002 0.0037 0.0002 0.0026 0.0007 0.0496 0.0024
Bud Site 0.0045 0.0057 0.0063 0 0.0004 0.0002 0.0003 0.0025 0.002 0.0005 0.0004 0.0003 0.0028 0.0135 0.0058 0.0001 0.0025 0.0011
Cell Periphery 0.0007 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0005 0.0003 0.0002 0 0.0013 0.0001
Cytoplasm 0.1081 0.1373 0.1013 0.0868 0.1091 0.1873 0.2346 0.2669 0.2622 0.3048 0.1568 0.2603 0.1766 0.2387 0.1748 0.1454 0.159 0.2491
Cytoplasmic Foci 0.0158 0.0082 0.0148 0.0037 0.0127 0.0247 0.0058 0.0101 0.0093 0.0086 0.0075 0.0153 0.0334 0.0054 0.0073 0.0055 0.0104 0.0173
Eisosomes 0.0004 0.0001 0.0004 0 0.0004 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0012 0.0002 0.0001 0 0.0006 0.0001
Endoplasmic Reticulum 0.0043 0.0034 0.0027 0.0007 0.0079 0.0015 0.0037 0.0037 0.0047 0.0037 0.0029 0.0024 0.0107 0.0031 0.0022 0.0012 0.0036 0.0061
Endosome 0.0127 0.0089 0.0075 0.0012 0.022 0.0035 0.0051 0.0078 0.0091 0.0042 0.0111 0.0049 0.0265 0.0025 0.0077 0.0016 0.0172 0.0115
Golgi 0.0038 0.0015 0.0027 0 0.0052 0.0005 0.0006 0.0006 0.0004 0.0002 0.0018 0.0001 0.0165 0.0006 0.0049 0.0001 0.018 0.0026
Lipid Particles 0.0068 0.0017 0.0061 0.0002 0.0086 0.006 0.0035 0.0028 0.0005 0.0018 0.0026 0.0003 0.0139 0.0004 0.002 0.0003 0.041 0.0072
Mitochondria 0.0033 0.0018 0.0127 0.0003 0.0022 0.0005 0.0006 0.0017 0.001 0.0009 0.0011 0.001 0.0076 0.0004 0.0025 0.0005 0.0331 0.0018
None 0.6782 0.6425 0.6053 0.8137 0.6445 0.5705 0.5978 0.5678 0.572 0.5262 0.7278 0.498 0.4858 0.5982 0.6551 0.6929 0.3977 0.5084
Nuclear Periphery 0.0145 0.0103 0.0119 0.0027 0.0398 0.0039 0.0061 0.0055 0.0053 0.0065 0.0068 0.0048 0.032 0.0036 0.0055 0.0031 0.0078 0.0109
Nucleolus 0.0025 0.0034 0.0039 0.0007 0.0041 0.0043 0.0016 0.0012 0.0011 0.0015 0.0011 0.0027 0.0011 0.0007 0.0011 0.0004 0.0007 0.0015
Nucleus 0.084 0.1403 0.1593 0.0785 0.0692 0.1509 0.1205 0.1143 0.1144 0.1273 0.0602 0.1837 0.065 0.1229 0.105 0.1401 0.1283 0.1366
Peroxisomes 0.004 0.0006 0.0038 0.0001 0.0024 0.0023 0.0019 0.0007 0.0002 0.0002 0.0011 0.0002 0.0231 0.0002 0.001 0.0009 0.0166 0.004
Punctate Nuclear 0.0121 0.0255 0.0353 0.0106 0.0338 0.0318 0.0127 0.0085 0.0097 0.0083 0.0093 0.0194 0.0159 0.0025 0.0113 0.0048 0.0056 0.0232
Vacuole 0.0047 0.0027 0.0026 0.0004 0.0019 0.0012 0.0024 0.0033 0.0026 0.0023 0.0009 0.0023 0.0049 0.0019 0.0022 0.0007 0.0015 0.0019
Vacuole Periphery 0.0014 0.0004 0.0007 0.0001 0.002 0.0002 0.0004 0.0006 0.0004 0.0004 0.0004 0.0006 0.0027 0.0001 0.0005 0.0002 0.0013 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
171 309 284 91 1440 2314 1394 426 1611 2623 1678 517

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 702.45 747.68 927.86 916.48 665.02 783.40 854.01 922.02 668.99 779.19 866.51 921.04
Standard Deviation 76.48 97.16 111.91 110.32 101.53 97.27 114.66 121.28 99.84 97.94 117.51 119.45
Intensity Change Log 2 — 0.090025 0.401512 0.383708 — 0.236351 0.360855 0.471400 — 0.163040 0.381883 0.427020

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000127 0.000776 0.000555 0.001083 0.000075 0.000217 0.000310 0.000616 0.000080 0.000283 0.000352 0.000698
Bud Neck 0.001827 0.014373 0.001987 0.012533 0.002635 0.010655 0.003766 0.004481 0.002549 0.011093 0.003465 0.005898
Bud Site 0.001450 0.012296 0.000864 0.026432 0.001147 0.005920 0.002917 0.012715 0.001179 0.006671 0.002570 0.015129
Cell Periphery 0.000184 0.000180 0.000152 0.000639 0.000194 0.000230 0.000122 0.000197 0.000193 0.000224 0.000127 0.000275
Cytoplasm 0.143347* 0.250496* 0.065108 0.217471* 0.201427* 0.283018* 0.115532* 0.276443* 0.195262* 0.279187* 0.106998* 0.266063*
Cytoplasmic Foci 0.077515 0.130922 0.008130 0.013949 0.023987 0.039924 0.001312 0.010733 0.029669 0.050644 0.002466 0.011299
Eisosomes 0.000079 0.000111 0.000070 0.000202 0.000032 0.000045 0.000021 0.000046 0.000037 0.000053 0.000029 0.000074
Endoplasmic Reticulum 0.001142 0.004004 0.008149 0.012920 0.001426 0.001125 0.004599 0.005200 0.001396 0.001464 0.005200 0.006559
Endosome 0.001347 0.015052 0.001476 0.002878 0.001079 0.003982 0.000565 0.005890 0.001107 0.005286 0.000719 0.005360
Golgi 0.000337 0.002489 0.000074 0.000227 0.000249 0.001160 0.000039 0.003640 0.000259 0.001316 0.000045 0.003040
Lipid Particles 0.009728 0.004797 0.002193 0.003329 0.001698 0.003248 0.000430 0.001764 0.002550 0.003430 0.000728 0.002039
Mitochondria 0.004437 0.006124 0.000485 0.000661 0.001069 0.005039 0.000692 0.001294 0.001426 0.005167 0.000657 0.001182
Mitotic Spindle 0.000462 0.004684 0.000163 0.006205 0.000497 0.004872 0.001935 0.003510 0.000493 0.004850 0.001635 0.003984
None 0.008951 0.005050 0.006662 0.009848 0.021424 0.012056 0.005665 0.019600 0.020100 0.011231 0.005833 0.017883
Nuclear Periphery 0.000735 0.000945 0.001446 0.001940 0.000900 0.001117 0.001225 0.001974 0.000882 0.001096 0.001263 0.001968
Nuclear Periphery Foci 0.000242 0.000851 0.000649 0.002249 0.000460 0.000449 0.000388 0.001482 0.000437 0.000497 0.000432 0.001617
Nucleolus 0.002480 0.003099 0.001992 0.001035 0.003551 0.003300 0.000584 0.000783 0.003438 0.003276 0.000822 0.000828
Nucleus 0.731341* 0.481554* 0.872519* 0.571729* 0.730981* 0.603591* 0.848580* 0.572363* 0.731019* 0.589215* 0.852631* 0.572252*
Peroxisomes 0.005930 0.013396 0.000336 0.005483 0.000569 0.003449 0.000081 0.000354 0.001138 0.004621 0.000124 0.001257
Vacuole 0.008124 0.046363 0.026829 0.108982 0.006358 0.015444 0.011149 0.074160 0.006546 0.019087 0.013803 0.080289
Vacuole Periphery 0.000216 0.002438 0.000160 0.000205 0.000242 0.001160 0.000089 0.002753 0.000239 0.001311 0.000101 0.002305

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.59 -6.61 -6.97 -1.59 -2.62 -1.98 -13.56 -4.39 -3.07 -2.28 -3.04 -15.34 -5.76 -3.62 -2.80
Bud Neck -5.79 -1.99 -3.53 1.73 -3.09 -7.84 -2.77 -4.25 3.74 -2.21 -9.08 -2.82 -5.19 3.90 -3.38
Bud Site -3.54 0.12 -1.88 -0.12 -1.91 -5.69 -2.00 -3.54 -1.71 -2.83 -6.66 -1.97 -4.02 -1.73 -3.37
Cell Periphery -0.01 0.76 -1.83 -1.86 -2.09 -0.81 1.75 0.86 3.08 -1.96 -0.80 1.75 -0.15 0.87 -2.83
Cytoplasm -5.20 4.65 -0.37 3.71 -3.82 -9.31 11.66 1.52 8.38 -6.64 -10.32 12.96 1.59 9.34 -7.58
Cytoplasmic Foci -3.47 6.14 5.89 12.09 -0.85 -5.71 11.67 8.01 14.91 -4.26 -6.79 12.68 9.74 18.45 -4.25
Eisosomes -1.88 -0.18 -2.53 -1.63 -2.51 -3.93 1.62 -9.29 -6.93 -11.65 -4.44 0.15 -7.26 -5.24 -7.54
Endoplasmic Reticulum -3.51 -10.78 -8.39 -6.45 -3.07 2.07 -15.56 -12.73 -13.25 -3.77 -0.42 -18.60 -14.68 -14.37 -4.78
Endosome -4.68 -1.79 -3.95 3.88 -3.02 -6.74 3.31 -2.54 0.69 -3.00 -8.09 1.91 -3.06 2.13 -3.37
Golgi -2.74 2.49 1.57 2.99 -3.14 -2.81 1.94 -1.20 0.04 -1.49 -3.49 2.19 -1.16 0.51 -1.51
Lipid Particles 1.08 1.64 1.45 2.32 -1.76 -4.26 7.34 0.24 4.23 -5.76 -1.57 3.47 1.20 4.41 -5.94
Mitochondria -0.43 1.26 1.22 2.79 -1.23 -5.60 1.17 0.43 6.05 -1.00 -5.06 1.74 1.26 6.69 -1.10
Mitotic Spindle -2.33 0.23 -1.36 -0.43 -1.38 -4.20 -2.59 -4.00 -1.33 -2.71 -4.68 -2.53 -4.05 -1.37 -3.02
None 1.59 1.12 1.38 -0.20 0.22 4.04 8.08 3.01 0.15 -2.61 4.27 8.07 3.45 0.31 -2.53
Nuclear Periphery -1.40 -6.63 -8.08 -7.32 -4.09 -1.61 -5.40 -10.78 -11.02 -8.48 -1.78 -6.51 -12.33 -12.70 -9.39
Nuclear Periphery Foci -3.71 -4.37 -6.81 -4.79 -4.97 0.07 -0.91 -7.19 -5.32 -6.68 -0.42 -2.20 -9.26 -6.63 -8.15
Nucleolus -0.37 1.53 3.73 5.21 3.59 0.46 4.71 4.51 8.52 -1.21 0.42 4.71 4.86 9.38 1.03
Nucleus 8.27 -4.65 4.77 -2.06 9.46 12.08 -11.66 8.35 0.58 16.45 14.23 -12.67 9.65 0.13 18.87
Peroxisomes -2.05 2.34 1.35 3.66 -1.12 -5.68 6.03 2.46 6.25 -1.80 -5.80 3.88 1.47 6.63 -1.61
Vacuole -7.70 -6.54 -8.49 -6.53 -6.72 -7.27 -7.84 -13.97 -13.07 -12.47 -10.05 -10.09 -16.22 -14.79 -14.12
Vacuole Periphery -2.63 0.67 0.52 2.66 -0.23 -3.36 3.63 -0.99 0.32 -1.21 -4.08 3.54 -0.98 0.80 -1.22
Standard name
Human Ortholog
Description Protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
Localization
Cell Percentages nucleus (57%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Irc4

Irc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Irc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available