Standard name
Human Ortholog
Description Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Vps21p-GFP; has potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of; Vab2p-GFP-fusion localizes to cytoplasm in punctate pattern
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0.05 0.05
Bud Neck 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.9 0.91 0.92 0.94 0.94 0.88 0.87 – 0.71 0.76 0.69 0.64 0.95 0.96 0.87 – – – 0.48 0.72 0.5 0.71 0.69 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 – – – 0.06 0 0.1 0.07 0.05 0.07
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.2 0.06 0 0 0 0.08 0.08 – 0.4 0.25 0.37 0.39 0 0 0 – – – 0.31 0.12 0.2 0.08 0.06 0.08
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0.05 0.06 0.06 – 0 0.05 0.05 0.07 0 0 0.05 – – – 0 0 0.06 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0.06 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 0 0 0 1 – 0 0 0 1 2 0 0 – – – 0 2 10 1 4 5
Bud 0 3 1 2 0 3 10 – 15 11 15 26 1 1 0 – – – 0 0 6 6 22 25
Bud Neck 0 2 22 2 7 8 26 – 7 14 11 12 1 2 1 – – – 0 1 4 2 2 4
Bud Site 0 0 0 0 0 0 0 – 4 1 2 5 0 0 0 – – – – – – – – –
Cell Periphery 0 2 0 2 1 1 2 – 0 7 0 2 1 0 0 – – – 0 1 3 0 0 3
Cytoplasm 45 215 414 282 333 458 586 – 379 441 375 453 178 250 270 – – – 36 167 229 291 298 297
Endoplasmic Reticulum 0 1 2 1 0 1 1 – 2 0 1 0 4 0 0 – – – 0 0 6 5 4 5
Endosome 1 1 5 0 5 18 11 – 2 3 9 2 2 5 26 – – – 4 10 44 27 20 32
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 1 0 – – – 1 0 0 1 1 1
Mitochondria 10 14 0 2 1 41 57 – 210 145 201 274 0 1 0 – – – 23 27 91 31 26 38
Nucleus 0 0 0 1 0 0 2 – 0 4 3 8 1 1 0 – – – 0 0 0 4 7 3
Nuclear Periphery 0 0 0 1 0 0 1 – 0 0 0 3 0 0 1 – – – 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 – – – 0 0 1 0 0 1
Peroxisomes 0 0 0 0 1 3 1 – 1 0 1 3 0 0 4 – – – 0 0 2 0 0 0
SpindlePole 0 4 23 9 19 33 40 – 22 31 27 49 1 4 17 – – – 1 5 27 15 18 18
Vac/Vac Membrane 2 1 3 3 3 10 7 – 6 10 9 5 1 1 3 – – – 4 7 13 17 13 21
Unique Cell Count 50 236 450 299 353 521 676 531 581 547 711 187 260 310 75 232 463 413 433 476
Labelled Cell Count 58 244 472 305 370 576 745 648 668 654 843 193 266 322 75 232 463 413 433 476


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.1 4.9 4.6 5.2 4.2 4.0 – 3.3 3.7 3.5 3.6 4.9 5.1 4.8 – – – 6.2 6.3 6.3
Std Deviation (1e-4) 0.9 0.5 1.4 2.0 2.2 1.6 1.2 – 1.2 1.0 0.8 0.8 1.6 1.5 1.5 – – – 2.3 2.4 2.3
Intensity Change (Log2) – – – -0.09 0.09 -0.23 -0.27 – -0.54 -0.41 -0.46 -0.43 0.0 0.06 -0.01 – – – 0.35 0.38 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3514 3.0878 3.8104 3.1889 3.9304 3.0895 4.1771 2.4365 2.4954 2.1205 2.15 2.5016 1.7055 -0.128 0.097 -0.6586 -0.4968 -0.6727
Actin 0.1183 0.0005 0.0206 0.029 0.1497 0.02 0.0601 0.002 0.0165 0.0306 0.0528 0.0055 0.0511 0.0055 0.0314 0.0004 0 0.0001
Bud 0.0019 0.0014 0.0013 0.0008 0.0013 0.0014 0.0021 0.002 0.0009 0.0067 0.0035 0.0001 0.0012 0.0002 0.0005 0.0001 0 0
Bud Neck 0.0189 0.0002 0.0027 0.0108 0.0009 0.0091 0.0133 0.0013 0.0004 0.0005 0.0072 0.0005 0.0157 0.0012 0.0032 0.0001 0 0.0001
Bud Periphery 0.0041 0.0039 0.002 0.0011 0.0023 0.004 0.0057 0.004 0.0018 0.0344 0.0091 0.0002 0.0013 0.0005 0.0005 0.0002 0 0
Bud Site 0.0241 0.0047 0.0274 0.0023 0.0032 0.0008 0.042 0.0048 0.0013 0.0007 0.0106 0.0001 0.0214 0.005 0.0419 0.0003 0 0
Cell Periphery 0.0018 0.0003 0.0007 0.0004 0.0002 0.0003 0.0018 0.0005 0.0003 0.0002 0.0013 0.0001 0.001 0.0009 0.0006 0.0002 0.0001 0
Cytoplasm 0.0387 0.0447 0.049 0.057 0.0307 0.0494 0.0311 0.0925 0.092 0.0781 0.0687 0.0667 0.0321 0.0914 0.1084 0.1302 0.015 0.0937
Cytoplasmic Foci 0.0893 0.0701 0.0854 0.1308 0.0917 0.1782 0.0649 0.1114 0.0811 0.1722 0.1928 0.1456 0.1833 0.0615 0.1266 0.1889 0.2563 0.1255
Eisosomes 0.0019 0.0003 0.0006 0.0006 0.0011 0.0001 0.0014 0.0002 0.0006 0.0004 0.0006 0.0002 0.0006 0.0011 0.0005 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0054 0.0011 0.0064 0.0016 0.0053 0.002 0.0127 0.0032 0.0047 0.0013 0.0024 0.0018 0.0028 0.0034 0.0073 0.0066 0.0004 0.0008
Endosome 0.0485 0.0205 0.0299 0.0861 0.0486 0.056 0.0571 0.0849 0.0561 0.0825 0.1325 0.0344 0.0833 0.0307 0.0683 0.1426 0.0071 0.0491
Golgi 0.0211 0.0012 0.0078 0.0079 0.023 0.0053 0.0107 0.0051 0.0094 0.0065 0.0208 0.0029 0.0257 0.0019 0.0063 0.0094 0.0004 0.0015
Lipid Particles 0.0334 0.0162 0.0162 0.057 0.0192 0.03 0.0566 0.0157 0.0196 0.0302 0.0373 0.0296 0.0675 0.0093 0.0377 0.0738 0.0557 0.0425
Mitochondria 0.0055 0.0016 0.011 0.0102 0.0298 0.0028 0.0071 0.0073 0.015 0.0025 0.0244 0.0008 0.0057 0.0173 0.002 0.0054 0 0.0002
None 0.5052 0.8229 0.699 0.568 0.517 0.6128 0.4779 0.6477 0.6688 0.5246 0.3974 0.6795 0.3405 0.7581 0.5381 0.3972 0.6617 0.6765
Nuclear Periphery 0.0129 0.0004 0.005 0.0025 0.012 0.0037 0.0556 0.0014 0.0064 0.0009 0.0011 0.0178 0.0045 0.001 0.0064 0.0028 0.0001 0.0004
Nucleolus 0.0028 0.0006 0.0013 0.0007 0.0008 0.0007 0.0047 0.0005 0.0004 0.0007 0.0004 0.0003 0.0065 0.0006 0.001 0.0004 0 0.0001
Nucleus 0.0028 0.0004 0.0034 0.0018 0.0024 0.0011 0.0435 0.0008 0.0012 0.0007 0.0007 0.001 0.1008 0.0006 0.0028 0.0009 0 0.0002
Peroxisomes 0.0279 0.0023 0.0178 0.0137 0.031 0.0093 0.0171 0.0023 0.0166 0.0166 0.0272 0.0075 0.0336 0.0051 0.0057 0.0153 0.0012 0.0061
Punctate Nuclear 0.0282 0.0013 0.0067 0.0111 0.0269 0.0038 0.0176 0.002 0.003 0.0059 0.0011 0.0037 0.0108 0.0013 0.0049 0.0027 0.0013 0.0011
Vacuole 0.0056 0.0049 0.0048 0.0052 0.0014 0.0082 0.012 0.0095 0.0028 0.0032 0.0062 0.0012 0.0091 0.0026 0.0049 0.0186 0.0003 0.0016
Vacuole Periphery 0.0017 0.0003 0.001 0.0014 0.0013 0.0011 0.005 0.0012 0.0011 0.0006 0.0018 0.0006 0.0014 0.0006 0.0008 0.0038 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.2491 6.9709 1.7242 7.7063 5.8977 3.8968 5.2862 6.7392 6.001 8.4988
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1989 1409 1478 1450 1588 2033 165 1553 3577 3442 1643 3003

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 643.49 647.16 802.11 798.37 610.63 714.49 806.82 779.48 628.90 686.93 802.58 788.60
Standard Deviation 71.16 88.67 96.28 128.95 61.35 75.11 109.69 106.14 68.95 87.45 97.72 118.08
Intensity Change Log 2 — 0.008205 0.317882 0.311140 — 0.226615 0.401948 0.352214 — 0.118681 0.359426 0.331285

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000157 0.000607 0.000127 0.001095 0.000232 0.000483 0.000186 0.000401 0.000190 0.000534 0.000133 0.000736
Bud Neck 0.037580 0.056981 0.029718 0.014421 0.025214 0.056862 0.010658 0.022310 0.032090 0.056910 0.027804 0.018500
Bud Site 0.004886 0.020991 0.021271 0.037118 0.004835 0.011789 0.013820 0.045655 0.004864 0.015556 0.020523 0.041533
Cell Periphery 0.000214 0.000151 0.000083 0.000289 0.000205 0.000155 0.000391 0.000198 0.000210 0.000153 0.000114 0.000242
Cytoplasm 0.322312* 0.295321* 0.411336* 0.389867* 0.364114* 0.326410* 0.217112* 0.450497* 0.340870* 0.313683* 0.391831* 0.421222*
Cytoplasmic Foci 0.259224* 0.271298* 0.032402 0.044495 0.302022* 0.272781* 0.094053 0.039110 0.278224* 0.272174* 0.038593 0.041710
Eisosomes 0.000307 0.000160 0.000025 0.000064 0.000344 0.000216 0.000398 0.000053 0.000324 0.000193 0.000063 0.000058
Endoplasmic Reticulum 0.003187 0.001108 0.001763 0.002808 0.000945 0.000666 0.001508 0.001364 0.002191 0.000847 0.001737 0.002061
Endosome 0.034526 0.055804 0.024872 0.031563 0.017636 0.034286 0.040349 0.027034 0.027028 0.043095 0.026426 0.029221
Golgi 0.004074 0.008680 0.000201 0.010282 0.005510 0.005856 0.000391 0.002287 0.004711 0.007012 0.000220 0.006147
Lipid Particles 0.026952 0.013508 0.002426 0.003055 0.028875 0.014498 0.009013 0.002046 0.027806 0.014093 0.003088 0.002533
Mitochondria 0.012690 0.028605 0.001945 0.009450 0.013090 0.019459 0.000386 0.009014 0.012867 0.023203 0.001789 0.009224
Mitotic Spindle 0.001315 0.004907 0.025626 0.055192 0.000715 0.003776 0.004519 0.037280 0.001049 0.004239 0.023507 0.045929
None 0.003187 0.004638 0.003944 0.004987 0.001910 0.002247 0.005534 0.003985 0.002620 0.003226 0.004104 0.004469
Nuclear Periphery 0.001183 0.000832 0.004931 0.002654 0.000770 0.000801 0.001196 0.001691 0.001000 0.000814 0.004556 0.002156
Nuclear Periphery Foci 0.000372 0.000462 0.001522 0.002426 0.000431 0.000270 0.004530 0.001147 0.000398 0.000348 0.001824 0.001764
Nucleolus 0.001849 0.003603 0.004875 0.001291 0.001257 0.002220 0.006083 0.001488 0.001586 0.002786 0.004996 0.001393
Nucleus 0.163561* 0.090454 0.369757* 0.194772* 0.178585* 0.144736* 0.488457* 0.226203* 0.170231* 0.122515 0.381677* 0.211027*
Peroxisomes 0.011657 0.028910 0.004159 0.007674 0.017171 0.023918 0.020656 0.004905 0.014105 0.025961 0.005816 0.006242
Vacuole 0.108223 0.107467 0.058598 0.178157 0.035000 0.075944 0.080393 0.118162 0.075716 0.088848 0.060787 0.147131
Vacuole Periphery 0.002543 0.005513 0.000419 0.008342 0.001140 0.002628 0.000367 0.005170 0.001920 0.003809 0.000413 0.006702

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.76 0.54 -3.16 -1.09 -3.22 -1.56 -0.80 -1.63 0.97 -0.67 -3.32 1.03 -3.55 -0.49 -3.86
Bud Neck -5.28 -0.57 10.66 13.43 9.59 -10.03 3.54 2.52 12.40 -2.07 -10.33 -1.68 9.34 18.36 8.20
Bud Site -10.92 -4.74 -7.81 -0.61 -2.05 -5.01 -1.46 -8.98 -6.24 -2.03 -10.88 -4.97 -11.94 -5.44 -3.58
Cell Periphery 2.01 4.81 1.09 -0.70 -3.78 1.82 -2.91 2.07 0.57 4.86 2.69 5.25 2.15 -0.31 -3.87
Cytoplasm 2.98 -3.28 0.25 -2.42 3.14 4.39 9.92 -2.18 -6.21 -10.86 4.41 0.47 -1.92 -5.76 -1.91
Cytoplasmic Foci -1.26 40.03 42.79 37.95 1.98 3.87 16.42 45.15 43.99 5.78 1.30 52.24 61.67 58.29 4.26
Eisosomes 7.86 15.74 13.33 12.72 -13.95 2.89 -0.34 6.56 12.75 6.54 5.72 11.58 11.91 16.37 -0.01
Endoplasmic Reticulum 5.30 1.81 -1.55 -11.00 -5.16 2.30 -3.59 -7.03 -10.61 0.97 6.01 -0.99 -2.87 -14.89 -2.53
Endosome -6.46 3.17 6.76 11.65 2.70 -7.75 -3.12 0.16 6.45 3.12 -8.32 -0.26 5.57 12.53 3.92
Golgi -5.07 9.73 0.26 3.63 -3.44 -0.22 7.46 6.22 7.33 -2.42 -3.57 12.06 3.68 6.43 -4.19
Lipid Particles 8.12 18.52 18.53 9.54 0.03 7.90 7.96 16.13 15.81 4.96 11.45 22.79 24.57 17.86 4.18
Mitochondria -6.40 9.43 5.59 10.01 -1.62 -3.49 10.66 4.97 8.22 -3.63 -7.04 13.21 7.40 12.98 -2.80
Mitotic Spindle -3.34 -8.58 -10.56 -9.41 -2.55 -3.70 -1.71 -9.43 -8.01 -4.55 -4.70 -8.85 -14.07 -12.38 -2.03
None -1.26 -0.75 -0.84 0.72 -0.23 -0.80 -1.39 -4.98 -3.53 0.41 -1.10 -2.27 -2.83 -1.04 -0.18
Nuclear Periphery 2.14 -13.81 -10.71 -11.82 5.17 -0.21 -3.14 -11.49 -10.23 -3.29 1.64 -14.53 -14.91 -15.46 6.98
Nuclear Periphery Foci -2.10 -5.68 -7.09 -6.59 -0.24 1.02 -3.07 -3.50 -4.22 2.81 0.09 -6.27 -7.90 -7.85 2.76
Nucleolus -3.92 -7.42 2.24 4.65 7.74 -2.88 -2.32 -2.68 -0.19 1.97 -4.59 -7.94 -1.29 3.14 7.63
Nucleus 12.76 -23.99 -8.81 -18.39 14.45 5.47 -9.35 -11.78 -16.71 5.70 11.13 -25.75 -14.93 -23.72 14.45
Peroxisomes -8.31 7.68 5.98 11.39 0.04 -3.48 -0.57 9.19 13.18 2.63 -8.67 7.13 11.02 17.19 2.21
Vacuole -0.40 5.06 -21.82 -20.89 -24.57 -13.87 -5.74 -25.74 -17.78 -2.91 -4.77 -3.04 -30.88 -27.72 -24.40
Vacuole Periphery -7.14 12.46 -0.85 2.57 -3.67 -6.55 6.16 -1.87 0.15 -2.89 -8.38 14.24 -1.72 1.62 -4.66
Standard name
Human Ortholog
Description Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Vps21p-GFP; has potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of; Vab2p-GFP-fusion localizes to cytoplasm in punctate pattern
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Vab2

Vab2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vab2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available