Standard name
Human Ortholog
Description Peroxisomal matrix protein; well-conserved in fungi; contains tripartite homology domain of thiamine pyrophosphate (TPP) enzymes; targeted to peroxisomes by Pex5p; contains low sequence identity with Pdc1p; mRNA identified as translated by ribosome profiling data

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.99 0.98 0.99 0.98 0.97 0.9 0.91 0.76 0.87 1.0 1.0 0.98 0.89 0.93 0.93 0.83 0.87 0.8 0.89 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0 0 0.1 0.08 0.35 0.13 0 0 0 0 0 0 0.08 0 0.12 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 1 1 0 2 0 0 1 1 0 0 0 1 0 0 3 2 2 0 0 0
Bud 0 0 0 0 0 0 1 2 1 6 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 4 10 12 8 10 7 3 2 8 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 3 1 10 3 3 5 1 1 1 1 1 0 0 0 7 5 11 0 0 0 0 0 0
Cytoplasm 235 204 192 248 356 397 452 223 221 207 295 45 50 101 150 201 229 228 186 147 12 39 95
Endoplasmic Reticulum 0 1 2 1 0 2 2 4 1 0 3 0 0 2 7 11 11 1 0 1 0 0 0
Endosome 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 0 0 0 0
Golgi 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Mitochondria 14 1 0 1 0 5 10 25 19 97 45 1 0 1 0 1 0 21 9 22 0 0 0
Nucleus 1 0 0 0 1 2 1 4 0 0 0 0 0 0 2 3 6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 4 2 1 3 3 0 0 0 0 0 0 2 0 0 0 0 0
Vac/Vac Membrane 0 1 0 1 1 1 5 4 6 2 3 0 0 0 15 11 13 6 6 4 0 0 0
Unique Cell Count 246 208 194 253 359 407 465 249 242 274 341 45 50 103 168 215 245 275 214 186 14 43 105
Labelled Cell Count 255 210 209 266 374 422 488 272 253 319 363 46 50 104 182 233 270 275 214 186 14 43 105


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.7 4.9 4.5 5.0 4.2 4.3 3.7 4.0 3.4 3.9 4.9 4.9 5.0 11.6 11.0 10.5 4.5 4.9
Std Deviation (1e-4) 0.4 1.0 0.7 1.2 1.6 0.7 1.0 0.9 1.0 1.0 1.2 1.0 0.9 1.0 2.1 1.6 1.8 1.5 1.7
Intensity Change (Log2) -0.11 0.03 -0.23 -0.19 -0.4 -0.29 -0.55 -0.34 -0.0 -0.01 0.03 1.24 1.17 1.1 -0.13 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 4.3 3.2 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1682 0.0215 0.0921 -0.1689 -0.1104 -0.1764 -0.9697 -0.6696 -0.707 -0.9869 -0.7052 -0.5476 -1.0483 -1.1673 -1.1262 -1.3508 -0.9021 -1.553
Actin 0.0151 0.0003 0.0188 0.0078 0.0026 0.0008 0.0172 0.0001 0.0161 0.0045 0.0254 0 0.0354 0.0008 0.0197 0 0.0894 0.0001
Bud 0.0011 0.0021 0.001 0.0018 0.0008 0.0007 0.0003 0 0.0001 0.0003 0.0008 0 0.0004 0.0013 0.0007 0 0.0555 0
Bud Neck 0.0071 0.0001 0.0031 0.0178 0.002 0.0007 0.0006 0 0.0003 0.0014 0.0019 0.0001 0.0007 0.0001 0.0003 0.0001 0.0005 0.0001
Bud Periphery 0.0028 0.0026 0.0019 0.0135 0.0035 0.0025 0.0009 0 0.0002 0.0007 0.0012 0 0.0006 0.0015 0.0007 0 0.0161 0
Bud Site 0.0086 0.0055 0.0092 0.0083 0.002 0.0005 0.0027 0.0002 0.0003 0.0036 0.017 0 0.0012 0.0035 0.0065 0 0.0012 0
Cell Periphery 0.0003 0.0003 0.0003 0.0011 0.0005 0 0.0008 0.0001 0.0001 0.0002 0.0003 0 0.0002 0.0001 0.0001 0 0.0003 0
Cytoplasm 0.0555 0.0921 0.07 0.0833 0.0913 0.0965 0.0429 0.0909 0.0848 0.0232 0.0693 0.1371 0.0835 0.1631 0.1487 0.3338 0.1445 0.2136
Cytoplasmic Foci 0.0124 0.0041 0.0138 0.0105 0.0116 0.0084 0.0059 0.0033 0.005 0.0299 0.0389 0.0048 0.0298 0.0028 0.0183 0.0057 0.0189 0.0108
Eisosomes 0.0003 0.0001 0.0003 0.0006 0.0003 0.0001 0.0005 0.0001 0.0003 0.0003 0.0003 0 0.0002 0.0001 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.0034 0.0006 0.0016 0.0011 0.0033 0.0004 0.0016 0.0012 0.0011 0.001 0.0013 0.0004 0.0039 0.0007 0.0011 0.0002 0.0019 0.0009
Endosome 0.0117 0.0009 0.0116 0.006 0.0216 0.0016 0.0097 0.0008 0.0029 0.0225 0.0187 0.0008 0.0336 0.001 0.0111 0.0005 0.0824 0.0011
Golgi 0.0047 0.0001 0.0021 0.0023 0.0026 0.0003 0.0026 0.0001 0.0011 0.0051 0.0091 0 0.0199 0.0004 0.006 0 0.0427 0
Lipid Particles 0.0111 0.0001 0.0029 0.0125 0.0148 0.0003 0.0091 0.0003 0.0014 0.049 0.029 0.0001 0.0149 0.0026 0.0052 0 0.0298 0.0001
Mitochondria 0.0048 0.0003 0.0046 0.0032 0.0099 0.0003 0.0073 0.0002 0.0021 0.0061 0.0049 0.0001 0.0209 0.0025 0.0035 0.0001 0.0181 0.0001
None 0.8311 0.8858 0.8309 0.7833 0.76 0.8795 0.8784 0.901 0.8705 0.8318 0.7247 0.855 0.6874 0.8147 0.7471 0.6587 0.4624 0.7717
Nuclear Periphery 0.0025 0.0006 0.0044 0.0011 0.0093 0.0008 0.0034 0.0003 0.001 0.001 0.0028 0.0001 0.0075 0.0003 0.0005 0.0001 0.0035 0.0002
Nucleolus 0.0026 0.0004 0.0011 0.005 0.011 0.0003 0.0009 0.0001 0.0085 0.0015 0.0017 0 0.0005 0.0009 0.0001 0 0.0006 0
Nucleus 0.0038 0.0018 0.0024 0.003 0.0115 0.0017 0.0016 0.0005 0.0013 0.0008 0.0017 0.0003 0.0097 0.0007 0.0005 0.0004 0.0099 0.0002
Peroxisomes 0.014 0.0001 0.0125 0.0265 0.0239 0.0006 0.0047 0.0001 0.0007 0.0127 0.0418 0 0.0199 0.0014 0.0277 0 0.0136 0
Punctate Nuclear 0.0039 0.0013 0.0054 0.0089 0.0093 0.0035 0.0056 0.0004 0.0014 0.0021 0.0073 0.0007 0.0234 0.0005 0.001 0.0001 0.0029 0.0009
Vacuole 0.0023 0.0006 0.0013 0.0021 0.0066 0.0003 0.0026 0.0004 0.0004 0.0018 0.0015 0.0003 0.0031 0.0007 0.0008 0.0002 0.0037 0.0002
Vacuole Periphery 0.0009 0.0001 0.0006 0.0004 0.0015 0.0001 0.0007 0 0.0003 0.0005 0.0007 0 0.0033 0.0001 0.0002 0 0.002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.8508 13.4799 7.3933 8.9236 8.9254 6.5525 10.5184 8.999 9.8124 9.6899
Translational Efficiency 0.3225 0.3402 0.4906 0.6338 0.3901 0.8336 0.5791 0.8846 0.6943 0.4885

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
546 306 1075 672 2048 1832 151 494 2594 2138 1226 1166

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 550.19 735.45 829.10 811.77 697.37 690.71 911.04 849.16 666.39 697.11 839.19 827.61
Standard Deviation 66.54 97.82 116.60 131.86 73.90 99.32 83.49 104.77 94.04 100.34 116.21 122.53
Intensity Change Log 2 0.418697 0.591616 0.561141 -0.013844 0.385590 0.284112 0.193027 0.480094 0.412882

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000039 0.000665 0.000352 0.000752 0.000233 0.000386 0.000258 0.000723 0.000192 0.000426 0.000340 0.000740
Bud Neck 0.011671 0.035331 0.003840 0.007058 0.013457 0.045205 0.004947 0.006040 0.013081 0.043792 0.003977 0.006627
Bud Site 0.002465 0.016807 0.004353 0.025947 0.003042 0.017453 0.009839 0.033302 0.002920 0.017360 0.005028 0.029063
Cell Periphery 0.000387 0.000176 0.000118 0.000239 0.000130 0.000193 0.000179 0.000183 0.000184 0.000190 0.000125 0.000215
Cytoplasm 0.464315* 0.293860* 0.314049* 0.515079* 0.496114* 0.397552* 0.363233* 0.520191* 0.489421* 0.382711* 0.320107* 0.517245*
Cytoplasmic Foci 0.155727 0.320592* 0.005661 0.025115 0.216607* 0.260206* 0.030059 0.026055 0.203793* 0.268849* 0.008666 0.025513
Eisosomes 0.000186 0.000214 0.000029 0.000050 0.000151 0.000167 0.000096 0.000062 0.000158 0.000174 0.000038 0.000055
Endoplasmic Reticulum 0.000693 0.001270 0.004696 0.001873 0.000867 0.000672 0.002701 0.001794 0.000830 0.000757 0.004450 0.001840
Endosome 0.003231 0.032835 0.001497 0.007163 0.009077 0.028282 0.007281 0.005289 0.007847 0.028934 0.002209 0.006369
Golgi 0.000531 0.005216 0.000072 0.013534 0.002501 0.006739 0.000250 0.000246 0.002086 0.006521 0.000094 0.007904
Lipid Particles 0.012201 0.010644 0.000987 0.002165 0.008424 0.008832 0.003020 0.001491 0.009219 0.009091 0.001238 0.001879
Mitochondria 0.002716 0.015193 0.000706 0.001335 0.003471 0.015554 0.000669 0.000574 0.003312 0.015502 0.000702 0.001013
Mitotic Spindle 0.000222 0.005306 0.004212 0.036245 0.003179 0.011588 0.013094 0.013686 0.002556 0.010689 0.005306 0.026688
None 0.014927 0.002813 0.004564 0.010213 0.002981 0.002813 0.005388 0.004700 0.005495 0.002813 0.004666 0.007877
Nuclear Periphery 0.000913 0.000633 0.001376 0.001133 0.000502 0.000436 0.001982 0.001150 0.000589 0.000464 0.001450 0.001140
Nuclear Periphery Foci 0.000196 0.000852 0.001136 0.001219 0.000273 0.000588 0.001871 0.001235 0.000256 0.000626 0.001227 0.001225
Nucleolus 0.002201 0.003013 0.000414 0.000857 0.000950 0.002108 0.000753 0.000387 0.001213 0.002237 0.000456 0.000658
Nucleus 0.307806* 0.111235 0.619405* 0.235793* 0.190803* 0.105033 0.408604* 0.225405* 0.215431* 0.105921 0.593442* 0.231392*
Peroxisomes 0.002871 0.037702 0.000346 0.000862 0.004513 0.015485 0.001845 0.001189 0.004168 0.018665 0.000531 0.001000
Vacuole 0.016018 0.103335 0.032033 0.108088 0.041820 0.077727 0.143638 0.155720 0.036389 0.081392 0.045779 0.128268
Vacuole Periphery 0.000683 0.002308 0.000154 0.005281 0.000907 0.002981 0.000294 0.000580 0.000860 0.002885 0.000171 0.003289

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.98 -11.67 -5.13 0.90 -0.28 -0.74 -0.50 -1.17 -0.46 -1.07 -1.38 -2.51 -2.29 -0.65 -0.26
Bud Neck -4.31 3.27 2.72 5.76 -0.82 -12.73 5.16 7.00 16.59 -0.11 -13.50 7.98 7.34 17.55 -0.61
Bud Site -5.43 -1.79 -4.47 0.50 -3.57 -9.26 -1.69 -3.05 0.94 -1.62 -10.38 -2.53 -5.43 1.13 -3.96
Cell Periphery 2.27 2.66 2.31 0.28 -0.90 -2.07 -1.79 0.73 2.28 2.03 -0.18 1.68 1.39 1.38 -0.03
Cytoplasm 9.30 13.50 4.97 -5.06 -7.92 11.42 10.29 10.58 3.23 -2.92 13.55 23.70 12.26 1.12 -8.37
Cytoplasmic Foci -10.59 20.53 18.68 23.01 -7.97 -6.14 31.85 41.08 43.26 2.50 -10.00 48.85 45.65 49.31 -7.31
Eisosomes -1.13 7.77 6.32 7.96 -6.55 -1.49 2.11 9.71 11.71 3.17 -1.55 14.38 12.14 14.65 -4.98
Endoplasmic Reticulum -2.18 -14.88 -8.19 -5.71 9.00 0.91 -7.31 -9.29 -8.59 2.41 0.34 -16.85 -12.52 -10.46 9.69
Endosome -7.57 2.91 -0.67 7.43 -3.21 -12.58 1.70 8.67 16.72 2.94 -14.89 11.48 8.16 18.32 -2.00
Golgi -2.75 5.53 -1.97 0.33 -2.23 -5.34 7.04 7.26 9.21 0.65 -6.20 7.72 -0.33 3.20 -2.23
Lipid Particles 0.70 7.95 7.52 4.78 -2.61 -0.49 7.16 12.43 11.28 4.20 0.18 14.62 14.23 12.03 -1.14
Mitochondria -2.90 5.27 5.14 3.37 -0.72 -8.03 5.14 6.57 10.54 0.75 -8.59 6.63 7.05 10.85 0.20
Mitotic Spindle -3.14 -2.80 -5.60 -4.47 -4.69 -4.33 -1.25 -3.45 -1.31 -1.22 -4.91 -1.41 -6.14 -3.56 -5.27
None 4.43 4.33 3.38 -2.72 -3.30 0.34 -0.30 -2.15 -2.20 -0.66 3.72 2.59 0.32 -4.31 -3.06
Nuclear Periphery 1.04 -4.35 -6.21 -9.23 -3.79 0.88 -9.84 -9.89 -10.26 0.73 1.58 -15.10 -15.27 -17.20 -3.73
Nuclear Periphery Foci -4.10 -10.76 -10.40 -2.45 1.71 -1.37 -5.96 -8.78 -3.98 1.08 -1.87 -11.77 -13.20 -4.01 1.31
Nucleolus -0.73 3.61 3.32 2.48 -1.00 -4.37 1.42 5.53 6.98 1.72 -3.64 5.74 5.35 6.83 -0.04
Nucleus 13.59 -23.00 1.43 -13.17 26.72 16.52 -9.18 -6.20 -13.82 5.73 21.41 -42.07 -7.43 -21.70 29.72
Peroxisomes -6.76 7.19 6.99 7.30 -0.50 -10.60 3.91 7.63 16.49 1.27 -12.61 8.76 9.00 17.07 0.22
Vacuole -10.83 -10.15 -21.86 -9.30 -17.26 -12.05 -9.39 -19.93 -17.04 -3.52 -16.25 -9.33 -28.15 -21.67 -20.91
Vacuole Periphery -3.20 2.69 -0.98 0.44 -1.48 -5.03 6.57 6.10 6.70 0.33 -5.59 8.91 -0.37 2.56 -1.50
Standard name
Human Ortholog
Description Peroxisomal matrix protein; well-conserved in fungi; contains tripartite homology domain of thiamine pyrophosphate (TPP) enzymes; targeted to peroxisomes by Pex5p; contains low sequence identity with Pdc1p; mRNA identified as translated by ribosome profiling data
Localization
Cell Percentages cytoplasm (46%), nucleus (8%), mixed (28%)
Cell Cycle Regulation Yes
nucleus - MA (0.00013)
Subcompartmental Group N/A

Pxp1

Pxp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pxp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available