Standard name
Human Ortholog
Description Protein with ubiquitin-like N terminus; subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover; Rad4p-Rad23p heterodimer binds to promoters of DNA damage response genes to repress their transcription in the absence of DNA damage
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.1 0.1 0.12 0.11 0.08 0.07 0.05 0.07 0 0.05 0.07 0.14 0.25 0.15 0.16 0.16 0 0 0.05 0.06 0.06 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.17 0.34 0.34 0.46 0.48 0.58 0.42 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.72 0.62 0.59 0.66 0.59 0.49 0.57 0.58 0.61 0.64 0.62 0.63 0.85 0.79 0.61 0.45 0.42 0.53 0.85 0.82 0.7 0.78 0.63 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.36 0.4 0.43 0.27 0.32 0.38 0.2 0.18 0.13 0.12 0.14 0.13 0.16 0.12 0.22 0.45 0.5 0.38 0.12 0.07 0.18 0.08 0.19 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 11 7 4 7 7 5 0 0 0 0 0 0 0 1 2 1 6 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 6 2 7 6 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 10 17 18 33 32 47 31 16 32 12 23 15 25 70 26 27 16 0 4 8 3 10 14
Endoplasmic Reticulum 0 0 1 0 0 0 1 0 0 0 0 1 0 1 2 7 9 9 0 0 0 0 1 0
Endosome 0 1 1 0 4 4 3 5 0 0 1 0 0 0 3 6 9 2 0 0 2 0 0 3
Golgi 0 0 0 0 2 0 0 0 0 0 0 0 0 1 1 2 3 0 0 2 1 1 0 0
Mitochondria 1 0 3 9 8 47 190 148 157 229 192 178 1 0 1 9 2 2 0 0 2 0 1 3
Nucleus 18 80 99 123 162 139 315 253 207 306 207 265 176 139 168 79 70 54 57 108 130 45 107 116
Nuclear Periphery 0 0 0 0 1 2 5 9 5 1 5 8 0 0 0 0 0 0 0 0 1 0 0 2
Nucleolus 0 1 0 2 1 1 10 3 5 3 5 4 0 0 0 0 0 0 0 0 0 0 1 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 3
Vac/Vac Membrane 9 52 73 50 87 107 113 79 45 59 45 55 33 22 60 78 84 38 8 9 34 4 32 47
Unique Cell Count 25 129 169 185 274 284 555 435 339 478 333 422 206 176 276 175 167 101 67 132 186 58 171 202
Labelled Cell Count 28 144 194 202 298 332 697 541 441 644 480 545 226 188 305 208 204 121 67 132 186 58 171 202


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.1 5.8 6.5 6.4 5.3 4.9 5.0 4.6 4.4 4.2 4.4 7.7 7.6 7.4 7.5 8.0 7.5 6.0 7.8 8.1
Std Deviation (1e-4) 0.5 1.1 1.2 2.6 2.3 1.4 1.5 2.1 1.3 1.1 1.2 1.1 1.1 1.4 1.4 1.9 2.2 1.6 0.6 1.2 1.6
Intensity Change (Log2) – – – 0.16 0.14 -0.13 -0.25 -0.21 -0.35 -0.39 -0.48 -0.41 0.41 0.39 0.35 0.37 0.47 0.36 0.04 0.42 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 0.6 0.4 -0.6 -1.2 -2.3 -1.4 -2.9 -2.0 -1.0 1.2 4.0 1.3 1.7 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.6 0 4.9 0 8.2 0 0 0 0 0 0 0 1.7 0 0
Nucleus 1.5 0.1 -2.0 -0.4 -0.1 0.5 1.3 0.8 1.0 5.9 4.1 0.5 -2.5 -3.1 -0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.2 -2.4 -1.2 -5.9 -6.4 -7.5 -8.6 -7.4 -8.0 -5.8 -6.4 -4.8 0.3 1.3 -0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.4569 6.3858 5.4118 5.3702 4.5827 5.4238 3.5479 5.5206 4.6669 4.3953 4.8074 5.1338 4.4534 5.5372 4.7136 4.5284 4.643 4.7124
Actin 0.0164 0 0.0004 0 0.0917 0.0019 0.0243 0 0.0044 0.0351 0 0.0326 0.0161 0.0001 0.0034 0.0109 0 0.0007
Bud 0.0003 0 0.0001 0 0.0001 0.0013 0.0013 0 0.0004 0.0006 0 0.0023 0.0012 0.0001 0.0002 0.0003 0 0.0003
Bud Neck 0.0008 0.0001 0.0002 0 0.0009 0.0011 0.0012 0 0.0002 0.0003 0.0004 0.0021 0.0011 0.0002 0.0008 0.0002 0.0001 0.0012
Bud Periphery 0.0005 0 0.0001 0 0.0002 0.0012 0.0017 0 0.0004 0.0006 0 0.0021 0.0037 0.0001 0.0002 0.0005 0 0.0003
Bud Site 0.0012 0.0001 0.0002 0 0.0004 0.0003 0.0036 0 0.0021 0.0011 0 0.0274 0.0018 0.0002 0.0047 0.0007 0 0.0007
Cell Periphery 0.0001 0 0 0 0.0001 0.0001 0.0004 0 0.0001 0.0001 0 0.0002 0.0006 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0037 0.008 0.0062 0.0002 0.0018 0.0027 0.0904 0.0022 0.0013 0.004 0.0009 0.0014 0.0019 0.0016 0.0131 0.0006 0.0016 0.0014
Cytoplasmic Foci 0.0015 0 0.0005 0 0.006 0.0005 0.0269 0 0.0079 0.0165 0 0.003 0.0121 0 0.0118 0.0207 0 0.0051
Eisosomes 0.0004 0 0 0 0.0005 0 0.0002 0 0 0.0004 0 0.0001 0.0013 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0026 0 0.0007 0 0.0009 0.0003 0.0094 0 0.0005 0.0023 0.0001 0.0002 0.0017 0.0002 0.0013 0.0008 0 0.0002
Endosome 0.0062 0 0.0016 0 0.0075 0.003 0.0536 0 0.0131 0.0206 0 0.006 0.0061 0.0001 0.0097 0.0223 0 0.0067
Golgi 0.0025 0 0.0003 0 0.0055 0.0014 0.0232 0 0.0042 0.0227 0 0.0148 0.0019 0 0.0057 0.0305 0 0.0033
Lipid Particles 0.0024 0 0.0012 0 0.0079 0.0009 0.0072 0 0.0048 0.0149 0 0.0026 0.0022 0 0.0066 0.0843 0 0.0009
Mitochondria 0.0053 0.0001 0.0036 0.0001 0.0008 0.0031 0.0102 0.0002 0.0081 0.0604 0.0003 0.0039 0.0152 0.0004 0.0069 0.014 0.0001 0.0016
None 0.0008 0.0007 0.0001 0 0.0051 0.0054 0.0058 0 0.0005 0.0014 0 0.0001 0.0009 0.0001 0.0008 0.0004 0 0.0001
Nuclear Periphery 0.0217 0.0031 0.0117 0.0018 0.011 0.0029 0.0332 0.0073 0.0069 0.0148 0.0069 0.0076 0.0093 0.0064 0.0052 0.0063 0.0019 0.0021
Nucleolus 0.0021 0.0021 0.002 0.0006 0.0035 0.0061 0.0042 0.0013 0.0046 0.004 0.0033 0.0038 0.0046 0.0031 0.0009 0.0009 0.0017 0.0031
Nucleus 0.9199 0.9854 0.964 0.9972 0.8043 0.9639 0.6577 0.988 0.9281 0.7487 0.9865 0.8848 0.9084 0.9859 0.9175 0.7835 0.9941 0.9697
Peroxisomes 0.0037 0 0.0045 0 0.0327 0.0002 0.0016 0 0.0018 0.0105 0 0.0028 0.0016 0 0.008 0.0174 0 0.0012
Punctate Nuclear 0.0064 0.0002 0.001 0.0001 0.0186 0.0016 0.0206 0.0003 0.0048 0.0388 0.0011 0.0006 0.0019 0.0004 0.0012 0.0021 0.0003 0.0008
Vacuole 0.0008 0.0001 0.0012 0 0.0002 0.0013 0.0165 0.0003 0.0033 0.0005 0.0002 0.0009 0.004 0.0006 0.001 0.001 0.0001 0.0006
Vacuole Periphery 0.0008 0 0.0005 0 0.0003 0.0008 0.0069 0.0001 0.0024 0.0016 0.0001 0.0005 0.0025 0.0003 0.0008 0.0025 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 92.1808 84.0521 103.9505 95.8599 75.2954 93.0511 110.1889 98.4103 116.2416 76.7464
Translational Efficiency 2.6828 3.6268 2.6468 2.9144 3.147 2.8323 2.0206 2.7267 1.9622 2.3483

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with ubiquitin-like N terminus; subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover; Rad4p-Rad23p heterodimer binds to promoters of DNA damage response genes to repress their transcription in the absence of DNA damage
Localization
Cell Percentages nucleus (46%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rad23

Rad23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available