Standard name | |
---|---|
Human Ortholog | |
Description | Guanosine diphosphatase located in the Golgi; involved in the transport of GDP-mannose into the Golgi lumen, converting GDP to GMP after mannose is transferred to substrates; null mutants are defective in sporulation and pre-meiotic S phase entry; orthologous to human ENTPD6, a meiosis-associated non-obstructive azoospermia (NOA) related gene identified in GWAS studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 1 |
Bud | 0 | 6 | 1 | 0 | 0 | 7 | 13 | 14 | 7 | 6 | 10 | 9 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 0 | 2 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 191 | 279 | 346 | 270 | 266 | 369 | 375 | 371 | 393 | 371 | 325 | 387 | 151 | 350 | 461 | 69 | 41 | 52 | 16 | 42 | 17 | 10 | 21 | 20 |
Endoplasmic Reticulum | 8 | 5 | 8 | 0 | 4 | 10 | 6 | 6 | 1 | 0 | 1 | 8 | 2 | 4 | 1 | 8 | 9 | 12 | 1 | 1 | 3 | 1 | 4 | 8 |
Endosome | 28 | 23 | 88 | 24 | 29 | 39 | 53 | 40 | 21 | 9 | 15 | 16 | 1 | 0 | 1 | 8 | 19 | 7 | 42 | 34 | 67 | 12 | 9 | 11 |
Golgi | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 7 | 0 | 3 | 0 |
Mitochondria | 18 | 26 | 6 | 15 | 28 | 133 | 141 | 155 | 128 | 184 | 140 | 93 | 2 | 0 | 0 | 15 | 3 | 8 | 16 | 17 | 27 | 9 | 11 | 15 |
Nucleus | 3 | 6 | 10 | 10 | 15 | 12 | 18 | 28 | 26 | 18 | 10 | 11 | 1 | 3 | 1 | 42 | 52 | 48 | 0 | 4 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 3 | 0 | 1 | 3 | 2 | 6 | 6 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 7 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 13 | 2 | 3 | 4 | 2 | 6 | 4 | 4 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 3 |
Vac/Vac Membrane | 199 | 222 | 344 | 233 | 325 | 320 | 328 | 312 | 236 | 264 | 169 | 222 | 13 | 14 | 9 | 96 | 101 | 115 | 332 | 415 | 603 | 111 | 255 | 248 |
Unique Cell Count | 367 | 481 | 656 | 413 | 514 | 724 | 754 | 749 | 658 | 644 | 533 | 609 | 166 | 364 | 468 | 187 | 180 | 188 | 424 | 536 | 745 | 149 | 316 | 328 |
Labelled Cell Count | 448 | 573 | 819 | 557 | 673 | 897 | 951 | 947 | 818 | 859 | 676 | 750 | 171 | 373 | 478 | 240 | 226 | 243 | 424 | 536 | 745 | 149 | 316 | 328 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.0 | 5.1 | 5.3 | 5.5 | 4.9 | 5.0 | 5.2 | 5.0 | 4.5 | 4.8 | 5.1 | 5.4 | 5.6 | 5.6 | 5.7 | 6.6 | 7.2 | 6.2 | 6.4 | 6.7 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.8 | 1.2 | 1.3 | 1.2 | 1.3 | 1.2 | 1.0 | 1.1 | 1.0 | 0.9 | 0.8 | 1.2 | 1.2 | 1.5 | 1.8 | 1.9 | 1.5 | 1.9 | 2.7 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.11 | -0.04 | -0.01 | 0.03 | -0.01 | -0.16 | -0.09 | 0.01 | 0.09 | 0.15 | 0.14 | 0.17 | 0.39 | 0.51 | 0.28 | 0.33 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 3.0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.1 | -0.3 | -0.7 | -1.1 | -1.2 | 2.6 | 1.8 | 2.8 | 3.9 | 9.0 | 14.3 | 16.8 | -3.8 | -7.1 | -6.1 |
Endoplasmic Reticulum | 0 | -0.7 | 0.3 | -0.8 | -0.8 | 0 | 0 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -4.0 | -4.4 | -5.2 | -4.0 | -5.2 | -6.7 | 0 | 0 | -7.0 | -4.7 | -7.3 | -8.1 | -3.5 | -1.0 | -3.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.1 | 4.6 | 10.8 | 10.9 | 11.6 | 11.1 | 0 | 0 | 9.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.1 | 1.6 | 0.2 | 1.2 | 2.6 | 2.7 | 1.6 | 0.5 | 0.4 | 0 | 0 | 0 | 10.5 | 12.4 | 11.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -2.0 | -2.0 | -2.4 | -3.1 | -1.9 | -2.2 | 0 | 0 | -2.7 | 0 | 0 | -3.1 | 0 | 0 | 0 |
Vacuole | 1.3 | 3.7 | -3.1 | -3.4 | -4.0 | -6.0 | -4.1 | -7.2 | -5.7 | -10.4 | -15.6 | -18.0 | -0.3 | 0.9 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5324 | 3.7055 | 3.6358 | 3.6223 | 6.2641 | 3.8009 | 5.0691 | 6.7711 | 6.3428 | 6.2947 | 7.1527 | 6.5196 | 5.4805 | 7.4091 | 7.0698 | 6.5164 | 6.7567 | 7.0452 |
Actin | 0.0127 | 0.0001 | 0.0078 | 0.0042 | 0.0263 | 0.0014 | 0.0286 | 0 | 0.0029 | 0.0094 | 0 | 0.0003 | 0.0103 | 0 | 0.0053 | 0.0145 | 0.0007 | 0 |
Bud | 0.0008 | 0.0005 | 0.0021 | 0.0001 | 0.0004 | 0.0005 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0002 |
Bud Neck | 0.002 | 0.0007 | 0.0014 | 0.0003 | 0.0026 | 0.0012 | 0.0009 | 0.0005 | 0.0009 | 0.0014 | 0.0003 | 0.0008 | 0.0027 | 0.0007 | 0.0005 | 0.0009 | 0.0006 | 0.0009 |
Bud Periphery | 0.0024 | 0.0006 | 0.001 | 0.0001 | 0.001 | 0.0012 | 0.001 | 0.0007 | 0.0003 | 0.0008 | 0.0003 | 0.0007 | 0.0004 | 0.0004 | 0.0006 | 0.0008 | 0.0005 | 0.0006 |
Bud Site | 0.0026 | 0.0042 | 0.0034 | 0.0004 | 0.0013 | 0.0005 | 0.0023 | 0.0008 | 0.0029 | 0.001 | 0.0003 | 0.0005 | 0.0008 | 0.0007 | 0.0008 | 0.0009 | 0.0004 | 0.0004 |
Cell Periphery | 0.0013 | 0.0008 | 0.0008 | 0.0001 | 0.0137 | 0.0009 | 0.001 | 0.0006 | 0.0004 | 0.0007 | 0.0002 | 0.0007 | 0.0005 | 0.0006 | 0.0004 | 0.0007 | 0.0005 | 0.0006 |
Cytoplasm | 0.0174 | 0.0289 | 0.0232 | 0.0082 | 0.0058 | 0.012 | 0.0104 | 0.0309 | 0.0217 | 0.0097 | 0.0037 | 0.0121 | 0.0083 | 0.0184 | 0.0116 | 0.0024 | 0.0038 | 0.0047 |
Cytoplasmic Foci | 0.0199 | 0.0178 | 0.0282 | 0.0134 | 0.017 | 0.0104 | 0.0257 | 0.0073 | 0.0175 | 0.0073 | 0.0061 | 0.0123 | 0.013 | 0.0039 | 0.0119 | 0.0072 | 0.0051 | 0.0049 |
Eisosomes | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0005 | 0 | 0.0001 | 0.0013 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0138 | 0.0051 | 0.0068 | 0.0031 | 0.043 | 0.0056 | 0.012 | 0.0066 | 0.0055 | 0.0032 | 0.0011 | 0.0078 | 0.0103 | 0.0099 | 0.007 | 0.0023 | 0.0051 | 0.0035 |
Endosome | 0.4017 | 0.3195 | 0.4137 | 0.532 | 0.3214 | 0.2905 | 0.4285 | 0.2804 | 0.4372 | 0.4974 | 0.387 | 0.359 | 0.3993 | 0.2329 | 0.3877 | 0.2814 | 0.4061 | 0.284 |
Golgi | 0.0168 | 0.0089 | 0.0337 | 0.0138 | 0.0388 | 0.0174 | 0.0337 | 0.0082 | 0.0386 | 0.0223 | 0.0518 | 0.0227 | 0.0189 | 0.0061 | 0.0291 | 0.0095 | 0.0121 | 0.0123 |
Lipid Particles | 0.0123 | 0.0032 | 0.0096 | 0.0135 | 0.0436 | 0.0051 | 0.0148 | 0.0014 | 0.0055 | 0.009 | 0.001 | 0.0094 | 0.0084 | 0.0017 | 0.0061 | 0.0024 | 0.0028 | 0.0031 |
Mitochondria | 0.0189 | 0.0055 | 0.0307 | 0.002 | 0.0678 | 0.0308 | 0.0252 | 0.0023 | 0.028 | 0.0248 | 0.0006 | 0.0177 | 0.0172 | 0.0027 | 0.0152 | 0.0338 | 0.0021 | 0.0084 |
None | 0.0146 | 0.0093 | 0.0264 | 0.0416 | 0.0038 | 0.0051 | 0.0023 | 0.0175 | 0.0116 | 0.0012 | 0 | 0.0026 | 0.0009 | 0.0003 | 0.0066 | 0.0001 | 0.0003 | 0.0001 |
Nuclear Periphery | 0.0196 | 0.0132 | 0.0096 | 0.0044 | 0.0213 | 0.0144 | 0.0145 | 0.0077 | 0.0041 | 0.0065 | 0.002 | 0.0101 | 0.0101 | 0.0124 | 0.0081 | 0.003 | 0.0198 | 0.0054 |
Nucleolus | 0.002 | 0.0009 | 0.0012 | 0.0006 | 0.0011 | 0.0013 | 0.0012 | 0.001 | 0.0006 | 0.0009 | 0.0002 | 0.0006 | 0.0005 | 0.0005 | 0.0005 | 0.0005 | 0.0007 | 0.0004 |
Nucleus | 0.0121 | 0.0043 | 0.0036 | 0.0011 | 0.0032 | 0.0033 | 0.0044 | 0.0038 | 0.002 | 0.0016 | 0.0008 | 0.002 | 0.0022 | 0.0023 | 0.0017 | 0.0011 | 0.0026 | 0.0014 |
Peroxisomes | 0.0066 | 0.0006 | 0.0067 | 0.0011 | 0.0239 | 0.0075 | 0.0017 | 0.0001 | 0.0023 | 0.0019 | 0.0001 | 0.0008 | 0.0029 | 0.0001 | 0.0008 | 0.0094 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.0046 | 0.0002 | 0.0044 | 0.0022 | 0.0118 | 0.0013 | 0.008 | 0.0002 | 0.0024 | 0.0005 | 0 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0 |
Vacuole | 0.3017 | 0.4691 | 0.2947 | 0.2576 | 0.2628 | 0.4468 | 0.2807 | 0.4754 | 0.3015 | 0.2879 | 0.4234 | 0.3798 | 0.3472 | 0.528 | 0.3418 | 0.4602 | 0.377 | 0.4957 |
Vacuole Periphery | 0.1155 | 0.1065 | 0.091 | 0.1001 | 0.0888 | 0.1428 | 0.1021 | 0.1542 | 0.1136 | 0.1108 | 0.1208 | 0.1594 | 0.1449 | 0.178 | 0.1635 | 0.1684 | 0.1588 | 0.173 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 137.1283 | 127.3357 | 104.8306 | 78.6937 | 113.0156 | 106.2422 | 130.1902 | 106.1352 | 90.3804 | 120.7159 |
Translational Efficiency | 1.2045 | 1.2834 | 0.9178 | 1.1423 | 0.9222 | 1.2575 | 0.9835 | 0.9284 | 0.9073 | 0.8395 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
117 | 1200 | 1334 | 620 | 2016 | 1352 | 375 | 71 | 2133 | 2552 | 1709 | 691 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 812.38 | 827.15 | 831.53 | 863.61 | 749.25 | 805.89 | 911.00 | 947.20 | 752.71 | 815.89 | 848.97 | 872.20 |
Standard Deviation | 153.38 | 114.43 | 104.65 | 129.82 | 97.14 | 104.61 | 99.36 | 130.48 | 102.05 | 109.85 | 108.61 | 132.34 |
Intensity Change Log 2 | — | 0.025994 | 0.033614 | 0.088225 | — | 0.105136 | 0.282004 | 0.338222 | — | 0.064507 | 0.158131 | 0.213583 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005962 | 0.000494 | 0.000424 | 0.001242 | 0.000115 | 0.000435 | 0.001635 | 0.000514 | 0.000435 | 0.000462 | 0.000689 | 0.001167 |
Bud Neck | 0.008863 | 0.011199 | 0.010449 | 0.014332 | 0.009938 | 0.014142 | 0.014666 | 0.009542 | 0.009879 | 0.012758 | 0.011374 | 0.013840 |
Bud Site | 0.048096 | 0.015150 | 0.009043 | 0.040218 | 0.010158 | 0.010677 | 0.016231 | 0.013116 | 0.012239 | 0.012780 | 0.010620 | 0.037433 |
Cell Periphery | 0.000392 | 0.000103 | 0.000301 | 0.000122 | 0.000259 | 0.000248 | 0.000083 | 0.000059 | 0.000267 | 0.000180 | 0.000253 | 0.000116 |
Cytoplasm | 0.124439* | 0.105983* | 0.352158* | 0.140426* | 0.039792 | 0.060001 | 0.113659* | 0.235971* | 0.044435 | 0.081623 | 0.299825* | 0.150244* |
Cytoplasmic Foci | 0.116818 | 0.255541* | 0.115539 | 0.137290 | 0.112416 | 0.158704 | 0.171001 | 0.177949 | 0.112657 | 0.204239* | 0.127709 | 0.141468 |
Eisosomes | 0.000121 | 0.000088 | 0.000123 | 0.000047 | 0.000063 | 0.000147 | 0.000085 | 0.000064 | 0.000066 | 0.000119 | 0.000115 | 0.000049 |
Endoplasmic Reticulum | 0.002529 | 0.003000 | 0.004094 | 0.005719 | 0.004796 | 0.004632 | 0.004474 | 0.001129 | 0.004671 | 0.003865 | 0.004177 | 0.005248 |
Endosome | 0.156167 | 0.210854* | 0.049800 | 0.156266 | 0.247015* | 0.251444* | 0.141161 | 0.120970 | 0.242032* | 0.232358* | 0.069847 | 0.152640 |
Golgi | 0.014901 | 0.043911 | 0.001580 | 0.015677 | 0.038451 | 0.037984 | 0.003131 | 0.000754 | 0.037159 | 0.040771 | 0.001920 | 0.014144 |
Lipid Particles | 0.017785 | 0.009800 | 0.014845 | 0.006606 | 0.006230 | 0.013487 | 0.018949 | 0.006073 | 0.006864 | 0.011753 | 0.015745 | 0.006551 |
Mitochondria | 0.047696 | 0.012304 | 0.001261 | 0.005828 | 0.019938 | 0.025465 | 0.001858 | 0.000220 | 0.021461 | 0.019276 | 0.001392 | 0.005252 |
Mitotic Spindle | 0.012305 | 0.004434 | 0.012825 | 0.057226 | 0.000725 | 0.001426 | 0.034481 | 0.016211 | 0.001360 | 0.002841 | 0.017577 | 0.053011 |
None | 0.000122 | 0.000193 | 0.005572 | 0.000355 | 0.000149 | 0.000208 | 0.000673 | 0.000618 | 0.000147 | 0.000201 | 0.004497 | 0.000382 |
Nuclear Periphery | 0.000695 | 0.000223 | 0.002444 | 0.002075 | 0.000175 | 0.000356 | 0.002802 | 0.001190 | 0.000204 | 0.000293 | 0.002523 | 0.001984 |
Nuclear Periphery Foci | 0.001485 | 0.000414 | 0.004774 | 0.005795 | 0.000179 | 0.000342 | 0.012454 | 0.003415 | 0.000250 | 0.000376 | 0.006459 | 0.005551 |
Nucleolus | 0.000689 | 0.000489 | 0.004264 | 0.000796 | 0.000402 | 0.000688 | 0.004580 | 0.000804 | 0.000417 | 0.000594 | 0.004333 | 0.000797 |
Nucleus | 0.011232 | 0.005812 | 0.203180* | 0.014014 | 0.002861 | 0.006393 | 0.068299 | 0.042044 | 0.003321 | 0.006120 | 0.173583* | 0.016894 |
Peroxisomes | 0.017273 | 0.008771 | 0.004640 | 0.017680 | 0.003267 | 0.009550 | 0.027700 | 0.035126 | 0.004035 | 0.009184 | 0.009700 | 0.019473 |
Vacuole | 0.401459* | 0.289651* | 0.200339* | 0.365170* | 0.447045* | 0.365993* | 0.359196* | 0.333366* | 0.444545* | 0.330096* | 0.235196* | 0.361902* |
Vacuole Periphery | 0.010973 | 0.021587 | 0.002349 | 0.013116 | 0.056028 | 0.037679 | 0.002883 | 0.000866 | 0.053556 | 0.030112 | 0.002466 | 0.011857 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.27 | 1.29 | 1.09 | -2.77 | -3.14 | -2.31 | -4.48 | -2.02 | 0.23 | 3.38 | -0.34 | -1.42 | -2.43 | -2.86 | -1.73 |
Bud Neck | -1.50 | -1.16 | -2.38 | -1.59 | -1.87 | -3.11 | -2.61 | 1.11 | 3.06 | 2.84 | -2.99 | -1.51 | -2.37 | -0.20 | -1.10 |
Bud Site | 2.44 | 2.98 | 0.90 | -4.84 | -6.61 | -0.47 | -2.17 | -0.09 | 0.07 | 1.12 | -0.68 | 1.37 | -5.73 | -5.55 | -6.24 |
Cell Periphery | 2.07 | 0.59 | 2.10 | 0.11 | 4.75 | 0.33 | 8.17 | 9.90 | 6.45 | 4.00 | 3.23 | 1.04 | 7.71 | 4.17 | 4.77 |
Cytoplasm | 2.01 | -9.82 | -0.93 | -5.92 | 15.55 | -5.13 | -9.90 | -6.35 | -5.70 | -3.01 | -9.82 | -35.99 | -14.28 | -10.08 | 11.83 |
Cytoplasmic Foci | -10.77 | -0.21 | 0.21 | 17.71 | 0.77 | -7.95 | -3.29 | -1.26 | 1.01 | 0.18 | -18.06 | -1.49 | 0.20 | 13.71 | 1.29 |
Eisosomes | 0.84 | -0.04 | 2.17 | 5.68 | 6.99 | -4.33 | -4.05 | 0.02 | 4.09 | 2.85 | -4.70 | -5.18 | 3.15 | 6.42 | 7.76 |
Endoplasmic Reticulum | -0.88 | -2.39 | -1.21 | -0.77 | 0.14 | 0.27 | -0.77 | 8.36 | 5.80 | 6.40 | 1.69 | 0.58 | 0.82 | 0.13 | 0.62 |
Endosome | -3.52 | 4.52 | 1.58 | 12.19 | -7.87 | -0.51 | 15.28 | 8.39 | 8.50 | 1.33 | 1.50 | 32.65 | 17.37 | 17.23 | -5.98 |
Golgi | -6.14 | 2.71 | 0.81 | 10.15 | -4.09 | 0.16 | 16.20 | 17.16 | 14.53 | 4.29 | -1.24 | 16.81 | 10.82 | 12.82 | -3.92 |
Lipid Particles | 0.98 | 0.30 | 1.70 | 4.21 | 5.52 | -5.59 | -4.12 | 0.84 | 4.84 | 4.12 | -5.39 | -5.75 | 1.52 | 6.72 | 6.69 |
Mitochondria | 1.87 | 2.61 | 2.44 | 5.21 | -2.23 | -2.36 | 12.83 | 15.53 | 13.00 | 1.90 | 0.98 | 13.49 | 10.16 | 10.37 | -2.04 |
Mitotic Spindle | 1.04 | -0.62 | -4.29 | -6.99 | -5.06 | -1.53 | -5.84 | -1.53 | -1.48 | 0.70 | -2.49 | -8.13 | -7.68 | -7.39 | -4.33 |
None | -1.11 | -5.09 | -2.94 | -2.56 | 4.71 | -1.35 | -4.04 | -1.77 | -1.47 | 1.94 | -1.59 | -5.36 | -3.28 | -2.75 | 4.87 |
Nuclear Periphery | 2.01 | -6.67 | -2.06 | -2.90 | 0.73 | -3.55 | -8.07 | -3.13 | -2.62 | 4.10 | -2.44 | -15.82 | -3.12 | -2.97 | 1.31 |
Nuclear Periphery Foci | 2.59 | -7.19 | -3.55 | -4.81 | 0.61 | -2.22 | -7.16 | -2.05 | -1.95 | 3.90 | -2.35 | -12.90 | -5.29 | -5.14 | 2.41 |
Nucleolus | 0.63 | -6.54 | -1.31 | -3.72 | 6.36 | -1.92 | -3.67 | -1.82 | -0.13 | 3.40 | -2.08 | -8.42 | -4.60 | -2.63 | 7.01 |
Nucleus | 2.22 | -24.72 | -3.03 | -8.15 | 24.30 | -3.49 | -10.37 | -4.57 | -4.23 | 2.48 | -3.79 | -30.09 | -10.26 | -8.98 | 23.73 |
Peroxisomes | 1.57 | 2.55 | 0.66 | -2.23 | -4.86 | -6.95 | -5.70 | -2.65 | -1.77 | -0.15 | -8.12 | -4.94 | -5.87 | -2.57 | -2.97 |
Vacuole | 4.18 | 6.65 | -0.71 | -10.60 | -16.66 | 7.21 | 3.67 | 1.05 | -1.16 | -0.58 | 12.43 | 20.64 | 0.07 | -8.57 | -14.40 |
Vacuole Periphery | -3.57 | 2.29 | 0.46 | 6.32 | -3.06 | 8.48 | 32.57* | 34.20* | 25.29 | 3.94 | 12.81 | 32.76 | 20.71 | 12.19 | -2.96 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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