Standard name
Human Ortholog
Description Threonine aldolase; catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.99 0.99 0.99 0.97 0.96 0.9 0.92 0.89 0.91 0.86 1.0 1.0 1.0 0.99 1.0 0.98 0.99 0.96 0.95 0.96 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.09 0.07 0.08 0.05 0.11 0 0 0 0.06 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 1 0 5 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 1 0 0 0 2 2 0 2 4 5 0 0 0 0 0 1 0 1 0 2 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 1 1 14 22 13 17 22 19 16 0 0 0 3 0 1 0 0 0 0 0 0
Cytoplasm 200 233 405 356 368 430 517 414 419 388 343 422 409 367 477 368 386 314 186 220 371 325 329 334
Endoplasmic Reticulum 0 0 1 2 1 8 17 40 30 36 19 54 0 0 0 24 26 17 0 0 3 0 1 1
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 1 0 3 0 0 1 0 2 2
Mitochondria 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 1 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 0 1 0 3 7 5
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 2 2 0 0 1 1 0 0 1 1 0 0 1 1 1 4 0 0 2 3 3 3
Unique Cell Count 200 237 409 358 370 442 541 462 456 437 378 490 410 367 478 370 387 319 189 230 393 339 354 364
Labelled Cell Count 201 240 413 361 371 452 559 472 469 449 396 501 410 367 478 397 413 340 189 230 393 339 354 364


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 194.1 278.1 195.8 182.7 156.7 130.2 109.1 105.3 93.5 85.9 75.3 74.7 213.7 200.3 179.8 231.3 207.5 188.3 200.5 197.2 205.4
Std Deviation (1e-4) 34.9 63.2 46.4 50.9 34.7 32.4 28.7 26.7 23.5 22.8 20.1 20.8 47.9 43.1 42.9 70.4 65.8 62.8 50.5 56.9 57.0
Intensity Change (Log2) – – – -0.1 -0.32 -0.59 -0.84 -0.89 -1.07 -1.19 -1.38 -1.39 0.13 0.03 -0.12 0.24 0.08 -0.06 0.03 0.01 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.9 3.5 2.6 3.2 4.0 3.9 3.0 0 0 0 0 0 0
Cytoplasm 0.7 0.7 -1.9 -3.1 -5.9 -4.9 -6.1 -5.3 -7.1 1.3 1.9 1.5 0.7 1.3 -0.7
Endoplasmic Reticulum 0 0 0 3.2 5.9 5.0 5.7 4.3 6.7 0 0 0 4.9 5.0 4.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 226.6722 298.6539 273.7681 244.523 234.8256 281.1815 219.1454 312.9258 282.8604 258.686 267.0178 287.9811 218.0733 293.7883 259.3969 219.1337 227.0721 217.4952
Actin 0.0012 0.0001 0.0001 0.0003 0.0052 0.0001 0.0059 0.0001 0.0033 0.0138 0.0046 0.0004 0.0164 0.0001 0.0009 0.0132 0.0082 0.0196
Bud 0.0007 0.0001 0.0003 0.0035 0.0005 0.0011 0.0008 0.0001 0.0011 0.0004 0.0007 0.0005 0.0009 0 0.0002 0.001 0.0055 0.0005
Bud Neck 0.0002 0.0001 0.0002 0.0003 0.0004 0.0006 0.0005 0.0001 0.0002 0.0009 0.0006 0.0005 0.0004 0.0001 0.0001 0.0341 0.0013 0.0002
Bud Periphery 0.0002 0 0.0002 0.001 0.0003 0.0003 0.0004 0 0.0002 0.0004 0.0002 0.0004 0.0006 0 0.0002 0.0008 0.0076 0.0011
Bud Site 0.0003 0 0.0001 0.0001 0.0002 0 0.0005 0 0.0002 0.0003 0.0005 0.0002 0.0039 0 0.0002 0.0005 0.0065 0.0005
Cell Periphery 0.0005 0.0002 0.0007 0.0004 0.0004 0.0001 0.0014 0 0.0007 0.0006 0.0012 0.0002 0.0008 0 0.0002 0.0008 0.0003 0.0002
Cytoplasm 0.8856 0.9887 0.9755 0.9447 0.8304 0.98 0.8608 0.9961 0.9534 0.9144 0.9198 0.9607 0.8643 0.9935 0.9512 0.9048 0.9099 0.7482
Cytoplasmic Foci 0.0078 0.0001 0.0004 0.0008 0.035 0.0002 0.0086 0.0001 0.0043 0.001 0.0065 0.0033 0.0165 0 0.0066 0.0032 0.002 0.0013
Eisosomes 0.0004 0 0 0 0.0006 0 0.001 0 0 0.0002 0.0004 0.0001 0.0009 0 0.0001 0.0006 0 0.0002
Endoplasmic Reticulum 0.0008 0.0001 0.0002 0.0001 0.0023 0.0002 0.0034 0 0.0008 0.0009 0.0013 0.0002 0.0036 0 0.0006 0.0002 0.0032 0.0178
Endosome 0.0045 0 0.0001 0 0.0246 0.0001 0.0072 0 0.0029 0.0021 0.0006 0.0003 0.0118 0 0.0018 0.0005 0.0052 0.0453
Golgi 0.0006 0 0 0 0.0057 0 0.0013 0 0.0005 0.0018 0.0001 0.0001 0.0038 0 0.0002 0.0008 0.0008 0.0077
Lipid Particles 0.0025 0 0.0001 0.0001 0.013 0 0.0033 0 0.0006 0.0006 0.0017 0.0003 0.0034 0 0.001 0.0005 0.0003 0.0056
Mitochondria 0.0066 0.0001 0.0021 0.0123 0.0098 0.0005 0.0078 0.0001 0.0012 0.0093 0.0027 0.0032 0.0057 0 0.0073 0.0072 0.0042 0.0108
None 0.0079 0.0003 0.0038 0.0025 0.0165 0.0002 0.0166 0.0002 0.003 0.0018 0.0153 0.0058 0.0086 0.0001 0.0052 0.0163 0.0009 0.0013
Nuclear Periphery 0.0096 0.0013 0.0022 0.008 0.0241 0.002 0.0227 0.0004 0.0083 0.0061 0.0082 0.0029 0.0142 0.0007 0.0038 0.0016 0.0098 0.1158
Nucleolus 0.026 0.0001 0.0023 0.0063 0.0069 0 0.0076 0 0.0007 0.0056 0.0104 0.0019 0.0036 0 0.0097 0.0014 0.0009 0.0004
Nucleus 0.039 0.0085 0.0082 0.0169 0.0127 0.013 0.0376 0.0026 0.0135 0.0288 0.0157 0.0164 0.0329 0.0052 0.007 0.01 0.0262 0.0102
Peroxisomes 0.0005 0 0.0001 0.0004 0.0009 0 0.0004 0 0.0008 0.0003 0.0003 0.0003 0.0022 0 0.0005 0.0005 0.0003 0.0003
Punctate Nuclear 0.002 0 0.0001 0.0002 0.0033 0.0001 0.0013 0 0.0014 0.0003 0.0009 0.0005 0.0027 0 0.0005 0.0004 0.0016 0.0028
Vacuole 0.0018 0.0002 0.0024 0.0012 0.0024 0.001 0.0063 0.0001 0.002 0.0037 0.0035 0.0011 0.0015 0.0001 0.001 0.0011 0.0039 0.0031
Vacuole Periphery 0.0013 0 0.0008 0.0008 0.0047 0.0004 0.0045 0 0.0006 0.0067 0.0046 0.0006 0.0012 0 0.0015 0.0004 0.0013 0.007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 343.629 397.4316 413.7563 381.3654 345.9771 330.6486 393.703 356.8033 388.7973 360.8952
Translational Efficiency 1.4132 1.6242 1.4153 1.7036 1.5818 1.5081 1.5491 1.6753 1.4083 1.3891

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
89 126 2200 1368 1540 1301 1287 1494 1629 1427 3487 2862

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 13560.41 19827.31 16729.86 18287.49 11333.94 15843.50 17105.14 16937.51 11455.58 16195.26 16868.37 17582.78
Standard Deviation 2998.78 5524.03 3339.07 5180.11 2713.25 4043.34 3678.07 4372.68 2776.13 4344.78 3472.78 4823.06
Intensity Change Log 2 — 0.548088 0.303025 0.431456 — 0.483242 0.593780 0.579572 — 0.518926 0.442698 0.500781

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000272 0.000080 0.000042 0.000392 0.000052 0.000041 0.000049 0.000100 0.000064 0.000045 0.000045 0.000240
Bud Neck 0.005080 0.000870 0.000463 0.001335 0.000332 0.000443 0.000902 0.000677 0.000591 0.000481 0.000625 0.000992
Bud Site 0.005867 0.001601 0.001204 0.003583 0.000782 0.000518 0.000537 0.002501 0.001060 0.000613 0.000958 0.003018
Cell Periphery 0.021325 0.006815 0.001970 0.003439 0.004469 0.000967 0.001227 0.001629 0.005390 0.001483 0.001695 0.002494
Cytoplasm 0.723898* 0.758019* 0.869619* 0.766520* 0.827179* 0.772553* 0.660728* 0.776278* 0.821536* 0.771270* 0.792520* 0.771614*
Cytoplasmic Foci 0.021070 0.023257 0.002598 0.008074 0.004901 0.005045 0.005951 0.006046 0.005784 0.006653 0.003836 0.007016
Eisosomes 0.000008 0.000003 0.000001 0.000005 0.000001 0.000001 0.000001 0.000002 0.000001 0.000001 0.000001 0.000003
Endoplasmic Reticulum 0.000053 0.000016 0.000017 0.000063 0.000049 0.000016 0.000012 0.000039 0.000049 0.000016 0.000015 0.000051
Endosome 0.000228 0.000031 0.000045 0.000481 0.000058 0.000042 0.000021 0.000041 0.000068 0.000041 0.000036 0.000251
Golgi 0.010327 0.001433 0.000476 0.000973 0.003174 0.001033 0.000314 0.000327 0.003565 0.001068 0.000416 0.000636
Lipid Particles 0.017624 0.003537 0.001155 0.001939 0.001585 0.001429 0.000722 0.001006 0.002461 0.001615 0.000995 0.001452
Mitochondria 0.000787 0.000118 0.000097 0.000344 0.000206 0.000182 0.000175 0.000331 0.000238 0.000176 0.000126 0.000337
Mitotic Spindle 0.001396 0.000250 0.000198 0.000964 0.000412 0.000248 0.000444 0.000614 0.000466 0.000248 0.000289 0.000782
None 0.009500 0.019346 0.005771 0.012054 0.009959 0.012346 0.008424 0.014767 0.009934 0.012964 0.006750 0.013470
Nuclear Periphery 0.001010 0.000306 0.000132 0.000221 0.000234 0.000194 0.000306 0.000150 0.000277 0.000204 0.000197 0.000184
Nuclear Periphery Foci 0.007129 0.001700 0.000846 0.001185 0.002132 0.001085 0.000556 0.000971 0.002405 0.001140 0.000739 0.001073
Nucleolus 0.000187 0.000076 0.000074 0.000144 0.000194 0.000070 0.000080 0.000091 0.000194 0.000071 0.000076 0.000116
Nucleus 0.122129 0.165986* 0.095838 0.166131* 0.131101* 0.198109* 0.310159* 0.178844* 0.130611* 0.195273* 0.174941* 0.172767*
Peroxisomes 0.000058 0.000007 0.000014 0.000031 0.000006 0.000004 0.000004 0.000014 0.000009 0.000004 0.000010 0.000022
Vacuole 0.042853 0.016068 0.019107 0.031162 0.009551 0.005052 0.009257 0.014928 0.011370 0.006025 0.015471 0.022688
Vacuole Periphery 0.009199 0.000482 0.000336 0.000959 0.003623 0.000623 0.000131 0.000644 0.003928 0.000610 0.000260 0.000794

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.30 1.72 0.37 -1.45 -2.19 0.48 -4.58 -5.45 -5.67 -2.70 1.07 -0.08 -2.57 -2.88 -2.61
Bud Neck 1.22 1.41 1.12 -1.01 -7.03 -3.55 -12.28 -11.05 -6.18 6.40 -0.15 -3.54 -4.18 -8.87 -1.33
Bud Site 0.89 1.30 0.61 -1.17 -4.45 0.86 -2.18 -5.11 -5.65 -3.29 0.96 -1.17 -5.53 -7.14 -5.63
Cell Periphery 2.87 4.25 4.01 2.38 -2.49 5.56 4.94 4.95 -1.23 0.26 5.70 5.53 4.85 -1.97 -1.51
Cytoplasm -0.50 -4.57 -0.67 -0.10 17.43 9.39 29.89 14.06 3.68 -16.58 9.01 12.20 16.02 4.68 4.25
Cytoplasmic Foci -1.00 3.89 2.45 3.33 -6.42 -2.17 -7.75 -5.36 -3.71 1.57 -3.01 -2.66 -5.95 -2.31 -4.18
Eisosomes 1.77 2.84 1.53 -0.79 -6.17 0.35 -6.28 -4.03 -4.17 0.77 0.96 -1.21 -5.64 -6.74 -5.39
Endoplasmic Reticulum 2.65 2.81 0.93 -1.12 -1.20 3.38 3.44 1.87 -0.82 -0.80 3.60 3.56 1.48 -1.51 -1.41
Endosome 2.02 1.89 -0.00 -1.08 -1.01 0.77 0.95 1.10 0.27 0.30 1.40 0.90 -0.81 -1.07 -0.95
Golgi 2.85 3.27 3.25 2.17 -0.10 5.84 8.32 8.61 5.00 1.68 6.43 8.60 8.53 4.00 0.80
Lipid Particles 2.43 3.03 2.60 0.34 -4.10 0.07 1.29 -1.38 -0.78 -2.89 0.88 2.35 -1.03 -1.64 -4.75
Mitochondria 1.41 1.44 1.19 -2.80 -3.60 0.14 0.15 -0.21 -0.28 -0.29 1.01 2.51 0.15 -0.86 -2.37
Mitotic Spindle 1.64 1.80 0.53 -2.03 -2.37 1.12 -0.02 -1.56 -1.83 -1.53 1.58 1.37 -2.02 -2.67 -2.61
None -1.74 2.22 0.58 2.11 -1.93 -1.42 3.68 -0.28 0.92 -2.85 -1.84 5.84 0.77 2.31 -3.73
Nuclear Periphery 2.50 3.35 2.93 1.40 -5.23 -0.40 -9.09 -1.07 -0.43 9.73 0.62 -1.59 -0.42 -1.24 2.01
Nuclear Periphery Foci 3.23 3.82 3.19 -0.16 -3.38 2.30 4.36 1.52 -1.19 -3.68 2.91 4.70 1.83 -1.76 -4.59
Nucleolus 1.21 1.34 0.90 -1.24 -3.31 1.07 0.59 0.89 -1.12 2.20 1.14 0.92 0.79 -2.19 -1.34
Nucleus -1.93 0.94 -2.39 0.15 -11.86 -11.84 -30.75 -11.44 1.46 20.15 -11.91 -13.73 -11.87 2.79 2.53
Peroxisomes 1.31 1.26 1.01 -2.29 -2.16 0.63 -0.35 -2.98 -5.13 -3.79 1.28 -0.47 -2.45 -5.54 -3.19
Vacuole 1.65 2.26 -1.58 -4.47 -10.01 1.77 -8.21 -11.28 -12.75 -2.87 1.63 -10.05 -17.46 -19.25 -9.24
Vacuole Periphery 2.17 2.33 2.28 0.72 -0.48 3.84 4.47 4.09 1.00 -0.62 4.26 4.78 4.50 0.95 -0.62
Standard name
Human Ortholog
Description Threonine aldolase; catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Gly1

Gly1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gly1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available