Standard name | |
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Human Ortholog | |
Description | Threonine aldolase; catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 2 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 1 | 1 | 14 | 22 | 13 | 17 | 22 | 19 | 16 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 200 | 233 | 405 | 356 | 368 | 430 | 517 | 414 | 419 | 388 | 343 | 422 | 409 | 367 | 477 | 368 | 386 | 314 | 186 | 220 | 371 | 325 | 329 | 334 |
Endoplasmic Reticulum | 0 | 0 | 1 | 2 | 1 | 8 | 17 | 40 | 30 | 36 | 19 | 54 | 0 | 0 | 0 | 24 | 26 | 17 | 0 | 0 | 3 | 0 | 1 | 1 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 1 | 0 | 2 | 2 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 7 | 5 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 4 | 0 | 0 | 2 | 3 | 3 | 3 |
Unique Cell Count | 200 | 237 | 409 | 358 | 370 | 442 | 541 | 462 | 456 | 437 | 378 | 490 | 410 | 367 | 478 | 370 | 387 | 319 | 189 | 230 | 393 | 339 | 354 | 364 |
Labelled Cell Count | 201 | 240 | 413 | 361 | 371 | 452 | 559 | 472 | 469 | 449 | 396 | 501 | 410 | 367 | 478 | 397 | 413 | 340 | 189 | 230 | 393 | 339 | 354 | 364 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 194.1 | 278.1 | 195.8 | 182.7 | 156.7 | 130.2 | 109.1 | 105.3 | 93.5 | 85.9 | 75.3 | 74.7 | 213.7 | 200.3 | 179.8 | 231.3 | 207.5 | 188.3 | 200.5 | 197.2 | 205.4 |
Std Deviation (1e-4) | 34.9 | 63.2 | 46.4 | 50.9 | 34.7 | 32.4 | 28.7 | 26.7 | 23.5 | 22.8 | 20.1 | 20.8 | 47.9 | 43.1 | 42.9 | 70.4 | 65.8 | 62.8 | 50.5 | 56.9 | 57.0 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.32 | -0.59 | -0.84 | -0.89 | -1.07 | -1.19 | -1.38 | -1.39 | 0.13 | 0.03 | -0.12 | 0.24 | 0.08 | -0.06 | 0.03 | 0.01 | 0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 2.9 | 3.5 | 2.6 | 3.2 | 4.0 | 3.9 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | 0.7 | -1.9 | -3.1 | -5.9 | -4.9 | -6.1 | -5.3 | -7.1 | 1.3 | 1.9 | 1.5 | 0.7 | 1.3 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.2 | 5.9 | 5.0 | 5.7 | 4.3 | 6.7 | 0 | 0 | 0 | 4.9 | 5.0 | 4.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 226.6722 | 298.6539 | 273.7681 | 244.523 | 234.8256 | 281.1815 | 219.1454 | 312.9258 | 282.8604 | 258.686 | 267.0178 | 287.9811 | 218.0733 | 293.7883 | 259.3969 | 219.1337 | 227.0721 | 217.4952 |
Actin | 0.0012 | 0.0001 | 0.0001 | 0.0003 | 0.0052 | 0.0001 | 0.0059 | 0.0001 | 0.0033 | 0.0138 | 0.0046 | 0.0004 | 0.0164 | 0.0001 | 0.0009 | 0.0132 | 0.0082 | 0.0196 |
Bud | 0.0007 | 0.0001 | 0.0003 | 0.0035 | 0.0005 | 0.0011 | 0.0008 | 0.0001 | 0.0011 | 0.0004 | 0.0007 | 0.0005 | 0.0009 | 0 | 0.0002 | 0.001 | 0.0055 | 0.0005 |
Bud Neck | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0006 | 0.0005 | 0.0001 | 0.0002 | 0.0009 | 0.0006 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0341 | 0.0013 | 0.0002 |
Bud Periphery | 0.0002 | 0 | 0.0002 | 0.001 | 0.0003 | 0.0003 | 0.0004 | 0 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0006 | 0 | 0.0002 | 0.0008 | 0.0076 | 0.0011 |
Bud Site | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0039 | 0 | 0.0002 | 0.0005 | 0.0065 | 0.0005 |
Cell Periphery | 0.0005 | 0.0002 | 0.0007 | 0.0004 | 0.0004 | 0.0001 | 0.0014 | 0 | 0.0007 | 0.0006 | 0.0012 | 0.0002 | 0.0008 | 0 | 0.0002 | 0.0008 | 0.0003 | 0.0002 |
Cytoplasm | 0.8856 | 0.9887 | 0.9755 | 0.9447 | 0.8304 | 0.98 | 0.8608 | 0.9961 | 0.9534 | 0.9144 | 0.9198 | 0.9607 | 0.8643 | 0.9935 | 0.9512 | 0.9048 | 0.9099 | 0.7482 |
Cytoplasmic Foci | 0.0078 | 0.0001 | 0.0004 | 0.0008 | 0.035 | 0.0002 | 0.0086 | 0.0001 | 0.0043 | 0.001 | 0.0065 | 0.0033 | 0.0165 | 0 | 0.0066 | 0.0032 | 0.002 | 0.0013 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0.0006 | 0 | 0.001 | 0 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0006 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0023 | 0.0002 | 0.0034 | 0 | 0.0008 | 0.0009 | 0.0013 | 0.0002 | 0.0036 | 0 | 0.0006 | 0.0002 | 0.0032 | 0.0178 |
Endosome | 0.0045 | 0 | 0.0001 | 0 | 0.0246 | 0.0001 | 0.0072 | 0 | 0.0029 | 0.0021 | 0.0006 | 0.0003 | 0.0118 | 0 | 0.0018 | 0.0005 | 0.0052 | 0.0453 |
Golgi | 0.0006 | 0 | 0 | 0 | 0.0057 | 0 | 0.0013 | 0 | 0.0005 | 0.0018 | 0.0001 | 0.0001 | 0.0038 | 0 | 0.0002 | 0.0008 | 0.0008 | 0.0077 |
Lipid Particles | 0.0025 | 0 | 0.0001 | 0.0001 | 0.013 | 0 | 0.0033 | 0 | 0.0006 | 0.0006 | 0.0017 | 0.0003 | 0.0034 | 0 | 0.001 | 0.0005 | 0.0003 | 0.0056 |
Mitochondria | 0.0066 | 0.0001 | 0.0021 | 0.0123 | 0.0098 | 0.0005 | 0.0078 | 0.0001 | 0.0012 | 0.0093 | 0.0027 | 0.0032 | 0.0057 | 0 | 0.0073 | 0.0072 | 0.0042 | 0.0108 |
None | 0.0079 | 0.0003 | 0.0038 | 0.0025 | 0.0165 | 0.0002 | 0.0166 | 0.0002 | 0.003 | 0.0018 | 0.0153 | 0.0058 | 0.0086 | 0.0001 | 0.0052 | 0.0163 | 0.0009 | 0.0013 |
Nuclear Periphery | 0.0096 | 0.0013 | 0.0022 | 0.008 | 0.0241 | 0.002 | 0.0227 | 0.0004 | 0.0083 | 0.0061 | 0.0082 | 0.0029 | 0.0142 | 0.0007 | 0.0038 | 0.0016 | 0.0098 | 0.1158 |
Nucleolus | 0.026 | 0.0001 | 0.0023 | 0.0063 | 0.0069 | 0 | 0.0076 | 0 | 0.0007 | 0.0056 | 0.0104 | 0.0019 | 0.0036 | 0 | 0.0097 | 0.0014 | 0.0009 | 0.0004 |
Nucleus | 0.039 | 0.0085 | 0.0082 | 0.0169 | 0.0127 | 0.013 | 0.0376 | 0.0026 | 0.0135 | 0.0288 | 0.0157 | 0.0164 | 0.0329 | 0.0052 | 0.007 | 0.01 | 0.0262 | 0.0102 |
Peroxisomes | 0.0005 | 0 | 0.0001 | 0.0004 | 0.0009 | 0 | 0.0004 | 0 | 0.0008 | 0.0003 | 0.0003 | 0.0003 | 0.0022 | 0 | 0.0005 | 0.0005 | 0.0003 | 0.0003 |
Punctate Nuclear | 0.002 | 0 | 0.0001 | 0.0002 | 0.0033 | 0.0001 | 0.0013 | 0 | 0.0014 | 0.0003 | 0.0009 | 0.0005 | 0.0027 | 0 | 0.0005 | 0.0004 | 0.0016 | 0.0028 |
Vacuole | 0.0018 | 0.0002 | 0.0024 | 0.0012 | 0.0024 | 0.001 | 0.0063 | 0.0001 | 0.002 | 0.0037 | 0.0035 | 0.0011 | 0.0015 | 0.0001 | 0.001 | 0.0011 | 0.0039 | 0.0031 |
Vacuole Periphery | 0.0013 | 0 | 0.0008 | 0.0008 | 0.0047 | 0.0004 | 0.0045 | 0 | 0.0006 | 0.0067 | 0.0046 | 0.0006 | 0.0012 | 0 | 0.0015 | 0.0004 | 0.0013 | 0.007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 343.629 | 397.4316 | 413.7563 | 381.3654 | 345.9771 | 330.6486 | 393.703 | 356.8033 | 388.7973 | 360.8952 |
Translational Efficiency | 1.4132 | 1.6242 | 1.4153 | 1.7036 | 1.5818 | 1.5081 | 1.5491 | 1.6753 | 1.4083 | 1.3891 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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