Standard name | |
---|---|
Human Ortholog | |
Description | Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; promotes association of TRAPPII-specific subunits with the TRAPP core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 27 | 4 | 6 | 1 | 2 | 0 | – | 1 | 0 | 1 | 0 | 0 | 29 | 17 | 8 | 6 | 14 | 16 | 19 | 7 | 2 | 9 | 13 | 20 |
Bud | 8 | 2 | 11 | 13 | 16 | 11 | – | 62 | 27 | 40 | 18 | 26 | 10 | 17 | 20 | 7 | 5 | 8 | 3 | 0 | 0 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 6 | 0 | 1 | 1 | 1 | 2 | – | 8 | 8 | 19 | 8 | 12 | 0 | 3 | 1 | 0 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 1 | 0 | 0 | 0 | – | 1 | 1 | 0 | 1 | 4 | 5 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 4 | 3 | 6 | 16 | 7 | – | 33 | 13 | 23 | 12 | 23 | 18 | 14 | 21 | 32 | 16 | 15 | 3 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 12 | 4 | 3 | 1 | 1 | 4 | – | 1 | 1 | 1 | 1 | 1 | 15 | 10 | 4 | 9 | 3 | 4 | 0 | 0 | 0 | 1 | 5 | 0 |
Endosome | 55 | 6 | 1 | 14 | 84 | 13 | – | 22 | 4 | 6 | 0 | 2 | 77 | 173 | 264 | 71 | 36 | 44 | 181 | 23 | 41 | 74 | 112 | 131 |
Golgi | 26 | 19 | 0 | 0 | 5 | 1 | – | 1 | 0 | 0 | 0 | 0 | 73 | 54 | 36 | 38 | 32 | 29 | 115 | 38 | 17 | 58 | 142 | 137 |
Mitochondria | 345 | 76 | 100 | 122 | 123 | 176 | – | 431 | 292 | 371 | 201 | 263 | 257 | 284 | 249 | 21 | 25 | 14 | 42 | 14 | 16 | 22 | 33 | 34 |
Nucleus | 1 | 0 | 1 | 0 | 3 | 2 | – | 1 | 2 | 1 | 1 | 1 | 0 | 2 | 1 | 5 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 2 | 2 | 0 | 0 | 0 | 3 | – | 1 | 4 | 1 | 2 | 0 | 5 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Nucleolus | 8 | 2 | 2 | 1 | 1 | 6 | – | 23 | 7 | 10 | 5 | 4 | 5 | 4 | 13 | 7 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 33 | 5 | 3 | 18 | 95 | 20 | – | 62 | 17 | 35 | 3 | 16 | 23 | 130 | 184 | 9 | 5 | 2 | 83 | 18 | 24 | 39 | 59 | 63 |
SpindlePole | 30 | 0 | 12 | 9 | 37 | 17 | – | 106 | 27 | 85 | 15 | 37 | 24 | 49 | 85 | 39 | 14 | 12 | 28 | 2 | 5 | 4 | 8 | 9 |
Vac/Vac Membrane | 18 | 2 | 0 | 0 | 2 | 2 | – | 4 | 7 | 5 | 6 | 2 | 14 | 14 | 22 | 22 | 13 | 13 | 10 | 3 | 1 | 4 | 6 | 5 |
Unique Cell Count | 440 | 94 | 112 | 143 | 247 | 202 | 552 | 319 | 452 | 224 | 289 | 406 | 567 | 680 | 195 | 123 | 126 | 499 | 111 | 111 | 222 | 392 | 410 | |
Labelled Cell Count | 581 | 126 | 144 | 187 | 386 | 264 | 759 | 410 | 599 | 274 | 392 | 555 | 775 | 913 | 268 | 167 | 163 | 499 | 111 | 111 | 222 | 392 | 410 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 6.9 | 4.4 | 5.1 | 5.3 | 4.0 | – | 4.1 | 3.2 | 3.5 | 3.3 | 3.4 | 6.2 | 6.3 | 6.2 | 9.5 | 9.8 | 9.0 | 6.5 | 6.7 | 6.4 |
Std Deviation (1e-4) | 0.8 | 1.0 | 0.8 | 1.0 | 1.2 | 1.0 | – | 1.6 | 0.7 | 1.0 | 0.7 | 5.2 | 0.7 | 0.8 | 0.9 | 1.8 | 2.0 | 1.5 | 1.2 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.2 | 0.26 | -0.13 | – | -0.12 | -0.46 | -0.34 | -0.44 | -0.36 | 0.49 | 0.51 | 0.48 | 1.1 | 1.14 | 1.02 | 0.56 | 0.59 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | -0.2 | -1.1 | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -2.2 | -1.7 | -1.0 |
Bud Neck | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 3.0 | 6.8 | – | – | 0 | – | 0 | 0 | 0 | 4.7 | 6.6 | 7.9 | 7.1 | 6.0 | 6.7 |
Golgi | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 4.8 | 3.4 | 2.5 | 5.0 | 5.8 | 5.4 |
Mitochondria | -0.9 | -7.1 | – | – | 0 | – | 0 | 0 | 0 | -5.3 | -7.6 | -10.4 | -13.6 | -10.6 | -12.0 |
Nuclear Periphery | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2.9 | 7.1 | – | – | 0 | – | 0 | 0 | 0 | 1.3 | 4.9 | 5.6 | 0 | 0 | 0 |
SpindlePole | -1.3 | 1.1 | – | – | 0 | – | 0 | 0 | 0 | -1.8 | -0.7 | 0.5 | 2.1 | 0.2 | -0.3 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 0.7 | 0 | 0 | 0 | 1.7 | 2.0 |
Cytoplasm | 0 | 1.5 | – | – | 1.4 | – | 1.1 | 1.1 | 1.9 | 0 | 0 | 0 | 3.6 | 2.9 | 2.7 |
Nucleus | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.5 | 3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9745 | 2.6173 | 2.5611 | 2.3504 | 2.0885 | 2.4616 | 1.5196 | 2.3555 | 2.1174 | 2.2911 | 1.3501 | 2.22 | 3.432 | 4.4308 | 3.9482 | 4.0149 | 3.0174 | 3.9463 |
Actin | 0.0119 | 0.0083 | 0.0056 | 0.0044 | 0.0117 | 0.0083 | 0.013 | 0.013 | 0.0072 | 0.004 | 0.0004 | 0.0178 | 0.023 | 0.0066 | 0.0061 | 0.0037 | 0.0024 | 0.0126 |
Bud | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.003 | 0.0029 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0001 |
Bud Neck | 0.0006 | 0.0005 | 0.0005 | 0.0013 | 0.0005 | 0.0005 | 0.0009 | 0.0014 | 0.0005 | 0.0003 | 0.0002 | 0.0012 | 0.0009 | 0.0006 | 0.0005 | 0.0014 | 0.0004 | 0.0011 |
Bud Periphery | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0011 | 0.002 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 |
Bud Site | 0.0009 | 0.0022 | 0.001 | 0.0009 | 0.0004 | 0.0002 | 0.0067 | 0.0193 | 0.002 | 0.0005 | 0.0005 | 0.0004 | 0.0023 | 0.0024 | 0.0006 | 0.0019 | 0.0013 | 0.0003 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0 |
Cytoplasm | 0.0029 | 0.0065 | 0.0052 | 0.0011 | 0.0012 | 0.0017 | 0.0078 | 0.0123 | 0.0054 | 0.0025 | 0.0016 | 0.0035 | 0.0164 | 0.0069 | 0.0062 | 0.0544 | 0.0029 | 0.002 |
Cytoplasmic Foci | 0.2288 | 0.2264 | 0.2237 | 0.2664 | 0.1934 | 0.1844 | 0.2157 | 0.2842 | 0.2513 | 0.2101 | 0.2841 | 0.2214 | 0.2097 | 0.2632 | 0.2441 | 0.21 | 0.1989 | 0.2458 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0004 | 0.0012 | 0.001 | 0.0001 | 0.0006 | 0.0006 | 0.0083 | 0.001 | 0.0015 | 0.0009 | 0.0002 | 0.0005 | 0.0013 | 0.0045 | 0.0003 | 0.0035 | 0.0003 | 0.0004 |
Endosome | 0.2372 | 0.2463 | 0.2089 | 0.2015 | 0.2356 | 0.1659 | 0.2348 | 0.2305 | 0.2057 | 0.3054 | 0.3041 | 0.1798 | 0.2221 | 0.2274 | 0.2304 | 0.2047 | 0.2316 | 0.1812 |
Golgi | 0.202 | 0.2414 | 0.2715 | 0.1699 | 0.2339 | 0.2888 | 0.2024 | 0.2222 | 0.3015 | 0.2565 | 0.2926 | 0.2834 | 0.2336 | 0.263 | 0.2955 | 0.2228 | 0.2621 | 0.3291 |
Lipid Particles | 0.085 | 0.0712 | 0.0423 | 0.0231 | 0.1252 | 0.0699 | 0.0588 | 0.0308 | 0.0403 | 0.0415 | 0.0319 | 0.0399 | 0.082 | 0.0283 | 0.0316 | 0.0235 | 0.1048 | 0.039 |
Mitochondria | 0.0168 | 0.025 | 0.0155 | 0.0047 | 0.0234 | 0.0147 | 0.0294 | 0.006 | 0.0115 | 0.0219 | 0.0021 | 0.0328 | 0.0104 | 0.0142 | 0.0047 | 0.0184 | 0.0045 | 0.0059 |
None | 0.0044 | 0.001 | 0.0004 | 0.0002 | 0.0005 | 0.0011 | 0.0024 | 0.0015 | 0.0014 | 0.0002 | 0.0001 | 0.001 | 0.0148 | 0.0003 | 0.0002 | 0.0029 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.001 | 0.0009 | 0.0052 | 0.0006 | 0.0014 | 0.0003 | 0.0001 | 0.0001 | 0.0073 | 0.0009 | 0.0001 | 0.0009 | 0.0005 | 0.0001 |
Nucleolus | 0.0018 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0.0088 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0006 | 0.0005 | 0 |
Nucleus | 0.0014 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.001 | 0.0012 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0051 | 0.0001 | 0 | 0.0018 | 0.0005 | 0 |
Peroxisomes | 0.1995 | 0.1597 | 0.2201 | 0.3239 | 0.1683 | 0.2594 | 0.2039 | 0.1504 | 0.1596 | 0.1499 | 0.0791 | 0.2156 | 0.1584 | 0.178 | 0.1773 | 0.2318 | 0.1792 | 0.18 |
Punctate Nuclear | 0.0034 | 0.0007 | 0.0007 | 0.0007 | 0.0018 | 0.001 | 0.002 | 0.0087 | 0.002 | 0.0004 | 0.0009 | 0.0009 | 0.0093 | 0.0008 | 0.0006 | 0.0006 | 0.0032 | 0.001 |
Vacuole | 0.0012 | 0.0063 | 0.0018 | 0.0008 | 0.0008 | 0.0009 | 0.0027 | 0.0029 | 0.0018 | 0.0025 | 0.0011 | 0.0008 | 0.0012 | 0.0013 | 0.0007 | 0.0144 | 0.0047 | 0.0005 |
Vacuole Periphery | 0.0008 | 0.0018 | 0.0009 | 0.0006 | 0.001 | 0.0006 | 0.0022 | 0.0008 | 0.001 | 0.0024 | 0.0006 | 0.0007 | 0.0011 | 0.0007 | 0.0007 | 0.0013 | 0.0017 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.6256 | 15.0428 | 14.3516 | 14.0382 | 24.9344 | 21.6234 | 15.7529 | 16.8648 | 15.6928 | 19.4264 |
Translational Efficiency | 1.5251 | 1.4549 | 1.1498 | 1.3478 | 0.8758 | 0.8821 | 1.5121 | 0.9369 | 1.0182 | 1.1183 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1979 | 1241 | 579 | 1353 | 1863 | 1478 | 1824 | 1264 | 3842 | 2719 | 2403 | 2617 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 765.66 | 806.16 | 1037.51 | 953.89 | 748.09 | 791.38 | 1001.04 | 955.40 | 757.14 | 798.13 | 1009.83 | 954.62 |
Standard Deviation | 88.69 | 207.09 | 113.11 | 127.58 | 119.91 | 102.00 | 128.10 | 122.33 | 105.36 | 159.01 | 125.62 | 125.08 |
Intensity Change Log 2 | — | 0.074362 | 0.438349 | 0.317119 | — | 0.081159 | 0.420216 | 0.352893 | — | 0.077725 | 0.429416 | 0.334909 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002329 | 0.004222 | 0.017460 | 0.023431 | 0.001646 | 0.001303 | 0.022482 | 0.005273 | 0.001998 | 0.002635 | 0.021272 | 0.014661 |
Bud Neck | 0.000793 | 0.000930 | 0.001282 | 0.003666 | 0.001845 | 0.000710 | 0.001027 | 0.004508 | 0.001303 | 0.000811 | 0.001089 | 0.004072 |
Bud Site | 0.001914 | 0.003551 | 0.011004 | 0.030743 | 0.003771 | 0.001969 | 0.009690 | 0.020714 | 0.002814 | 0.002691 | 0.010007 | 0.025899 |
Cell Periphery | 0.000039 | 0.000071 | 0.000079 | 0.000069 | 0.000054 | 0.000099 | 0.000041 | 0.000038 | 0.000046 | 0.000086 | 0.000050 | 0.000054 |
Cytoplasm | 0.003101 | 0.002698 | 0.005173 | 0.016382 | 0.004816 | 0.002928 | 0.003482 | 0.016869 | 0.003933 | 0.002823 | 0.003889 | 0.016617 |
Cytoplasmic Foci | 0.242959* | 0.301394* | 0.434121* | 0.317080* | 0.230474* | 0.254382* | 0.407043* | 0.333914* | 0.236905* | 0.275839* | 0.413568* | 0.325211* |
Eisosomes | 0.000059 | 0.000055 | 0.000071 | 0.000068 | 0.000090 | 0.000054 | 0.000055 | 0.000031 | 0.000074 | 0.000055 | 0.000059 | 0.000050 |
Endoplasmic Reticulum | 0.000050 | 0.000180 | 0.001616 | 0.000569 | 0.000131 | 0.000159 | 0.000553 | 0.000308 | 0.000089 | 0.000169 | 0.000809 | 0.000443 |
Endosome | 0.136295 | 0.200098* | 0.143990 | 0.190626 | 0.201641* | 0.199360 | 0.158577 | 0.235304* | 0.167982 | 0.199697 | 0.155063 | 0.212205* |
Golgi | 0.465871* | 0.370970* | 0.158039* | 0.134094* | 0.412099* | 0.397764* | 0.132888* | 0.104055 | 0.439797* | 0.385535* | 0.138948* | 0.119585* |
Lipid Particles | 0.015460 | 0.018175 | 0.033970 | 0.005448 | 0.015194 | 0.022865 | 0.010836 | 0.004705 | 0.015331 | 0.020725 | 0.016410 | 0.005090 |
Mitochondria | 0.049235 | 0.014348 | 0.000844 | 0.009411 | 0.048845 | 0.027088 | 0.003140 | 0.007691 | 0.049046 | 0.021273 | 0.002587 | 0.008580 |
Mitotic Spindle | 0.006791 | 0.008220 | 0.118581* | 0.097260 | 0.010635 | 0.005321 | 0.117369* | 0.105018* | 0.008655 | 0.006644 | 0.117661* | 0.101007* |
None | 0.000119 | 0.000065 | 0.000024 | 0.000037 | 0.000318 | 0.000124 | 0.000019 | 0.000030 | 0.000216 | 0.000097 | 0.000020 | 0.000033 |
Nuclear Periphery | 0.000016 | 0.000029 | 0.000163 | 0.000292 | 0.000040 | 0.000029 | 0.000109 | 0.000136 | 0.000028 | 0.000029 | 0.000122 | 0.000216 |
Nuclear Periphery Foci | 0.000150 | 0.000490 | 0.003602 | 0.000806 | 0.000432 | 0.000292 | 0.001351 | 0.000754 | 0.000287 | 0.000382 | 0.001894 | 0.000781 |
Nucleolus | 0.000084 | 0.000681 | 0.000103 | 0.000250 | 0.000373 | 0.000516 | 0.000061 | 0.000452 | 0.000224 | 0.000591 | 0.000071 | 0.000348 |
Nucleus | 0.000490 | 0.000416 | 0.000696 | 0.000382 | 0.001380 | 0.000264 | 0.000157 | 0.000324 | 0.000921 | 0.000333 | 0.000287 | 0.000354 |
Peroxisomes | 0.071624 | 0.069582 | 0.056149 | 0.135681* | 0.061054 | 0.080386 | 0.120884* | 0.125113* | 0.066499 | 0.075455 | 0.105286* | 0.130577* |
Vacuole | 0.001515 | 0.002252 | 0.012094 | 0.031058 | 0.003435 | 0.002420 | 0.009598 | 0.031767 | 0.002446 | 0.002343 | 0.010199 | 0.031400 |
Vacuole Periphery | 0.001106 | 0.001571 | 0.000939 | 0.002646 | 0.001728 | 0.001967 | 0.000636 | 0.002996 | 0.001407 | 0.001786 | 0.000709 | 0.002815 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.70 | -6.20 | -8.74 | -7.56 | -1.72 | 1.00 | -11.36 | -3.29 | -3.71 | 8.36 | -1.16 | -12.64 | -9.26 | -8.57 | 3.51 |
Bud Neck | -0.67 | -2.64 | -6.21 | -5.81 | -5.09 | 3.03 | 1.98 | -5.25 | -8.00 | -7.36 | 2.35 | 0.67 | -8.01 | -9.82 | -9.02 |
Bud Site | -1.83 | -5.24 | -13.57 | -12.24 | -7.54 | 2.27 | -5.86 | -9.39 | -10.75 | -6.11 | 0.20 | -8.87 | -16.38 | -16.41 | -10.46 |
Cell Periphery | -2.09 | -2.61 | -3.59 | 0.29 | 0.79 | -0.93 | 1.75 | 2.13 | 1.23 | 0.85 | -1.50 | -1.02 | -1.60 | 1.19 | -0.51 |
Cytoplasm | 0.23 | -1.54 | -9.45 | -9.19 | -6.31 | 1.48 | 0.62 | -7.75 | -8.66 | -8.55 | 1.23 | -0.93 | -12.06 | -12.60 | -11.75 |
Cytoplasmic Foci | -9.71 | -19.95 | -10.02 | -0.96 | 11.68 | -4.44 | -27.30 | -13.94 | -9.74 | 10.61 | -9.67 | -33.55 | -16.83 | -7.66 | 15.71 |
Eisosomes | 0.72 | -2.21 | -2.16 | -2.66 | 0.45 | 1.20 | 1.16 | 1.94 | 6.61 | 7.66 | 1.30 | 0.98 | 1.55 | 1.23 | 2.72 |
Endoplasmic Reticulum | -4.76 | -4.27 | -4.56 | -3.44 | 2.68 | -0.87 | -4.50 | -2.58 | -2.02 | 2.35 | -3.47 | -6.37 | -5.23 | -3.99 | 2.92 |
Endosome | -10.46 | -1.06 | -7.84 | 2.15 | -5.61 | 0.36 | 7.58 | -3.46 | -3.72 | -10.30 | -7.07 | 3.35 | -7.74 | -1.02 | -10.40 |
Golgi | 11.47 | 37.25* | 49.56* | 31.71* | 3.79 | 1.79 | 44.60* | 48.03* | 42.91* | 7.10 | 9.38 | 63.37* | 68.53* | 52.29* | 6.14 |
Lipid Particles | -1.00 | -4.57 | 5.94 | 5.46 | 7.43 | -2.75 | 2.47 | 6.61 | 7.49 | 5.86 | -2.76 | -0.83 | 8.84 | 9.24 | 9.36 |
Mitochondria | 11.04 | 17.65 | 12.62 | 2.37 | -4.91 | 6.07 | 16.36 | 12.99 | 7.10 | -2.44 | 11.48 | 23.58 | 18.10 | 7.13 | -4.62 |
Mitotic Spindle | -1.16 | -12.35 | -16.08 | -15.67 | 1.72 | 4.17 | -20.21* | -16.24 | -17.33 | 1.55 | 2.32 | -24.07 | -22.84 | -23.35 | 2.54 |
None | 1.42 | 2.54 | 2.18 | 1.42 | -1.07 | 1.37 | 2.29 | 2.35 | 2.16 | 0.15 | 1.66 | 2.87 | 2.92 | 2.46 | -0.13 |
Nuclear Periphery | -1.98 | -4.96 | -1.54 | -1.47 | -0.65 | 0.87 | -3.68 | -5.85 | -6.52 | -1.70 | -0.16 | -5.94 | -2.24 | -2.23 | -1.05 |
Nuclear Periphery Foci | -2.31 | -4.51 | -4.64 | -2.05 | 3.60 | 0.80 | -2.51 | -1.36 | -3.62 | 1.83 | -0.37 | -5.45 | -3.56 | -3.91 | 3.82 |
Nucleolus | -1.78 | -0.92 | -1.68 | 1.15 | -1.34 | -1.14 | 2.12 | -0.82 | 0.58 | -2.30 | -2.09 | 1.55 | -1.52 | 1.25 | -2.54 |
Nucleus | 0.19 | -0.64 | -0.15 | -0.53 | 0.72 | 1.85 | 1.81 | 1.38 | -1.78 | -1.57 | 1.64 | 1.42 | 1.06 | -1.47 | -0.93 |
Peroxisomes | 0.40 | 2.60 | -9.37 | -8.95 | -10.17 | -3.75 | -10.36 | -10.14 | -6.16 | -0.24 | -2.42 | -8.53 | -13.72 | -10.62 | -4.09 |
Vacuole | -2.64 | -5.93 | -13.17 | -12.94 | -7.53 | 1.86 | -7.84 | -12.77 | -13.07 | -10.34 | 0.28 | -10.70 | -18.33 | -18.38 | -13.54 |
Vacuole Periphery | -1.77 | 0.56 | -2.85 | -1.95 | -3.15 | -0.65 | 7.56 | -2.00 | -1.37 | -4.07 | -1.62 | 5.17 | -3.43 | -2.22 | -5.49 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|