Standard name
Human Ortholog
Description Protein that may act as a chaperone for cytochrome c oxidase subunits; conserved protein; may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0.05 0.07 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.1 0.1 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0.05 0.16 0.11 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.11 0.16 0.42 0.4 0 0.1 0.07 0.05 0.09 0
Mitochondria 0.97 0.92 0.95 0.94 0.94 0.91 0.96 0.89 0.96 0.97 0.96 0.96 0.88 0.87 0.84 0 0.1 0.05 0.85 0.76 0.74 0.78 0.73 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.09 0 0.05 0 0 0.09 0.1 0.09 0.06 0.1 0.08 0.07 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Nucleolus 0.11 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0.05 0.06 0 0.11 0.09 0.09 0.09
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.13 0.06 0.08 0.09 0.07 0.07 0 0 0 0 0 0.09 0.11 0.1 0.26 0.2 0.22 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 0 0 0 0 0 0 0 0 0 2 1 0 1 3 1 3 3 1 3 7
Bud 0 2 7 1 2 3 6 7 9 21 7 17 2 1 2 7 6 3 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 1 3 3 2 6 1 0 0 0 0 1 0 1
Cell Periphery 1 19 2 2 1 1 0 0 2 2 2 5 7 10 7 21 8 6 0 0 0 0 0 0
Cytoplasm 2 4 2 4 5 4 2 14 5 3 5 6 9 12 15 21 9 6 0 0 0 0 0 0
Endoplasmic Reticulum 1 2 5 1 0 3 2 1 1 1 2 2 4 4 5 2 2 1 0 0 0 0 0 0
Endosome 1 5 0 2 1 3 0 0 0 0 0 0 4 9 7 6 3 2 2 9 3 4 7 10
Golgi 0 11 0 0 2 1 0 0 0 0 1 0 12 27 31 22 34 23 3 24 10 8 21 10
Mitochondria 125 232 144 93 119 103 196 183 205 350 225 261 217 307 248 6 8 3 103 194 111 137 185 172
Nucleus 0 1 0 1 1 1 3 3 1 4 3 4 0 0 0 2 0 0 0 1 0 0 1 0
Nuclear Periphery 11 6 8 1 5 10 21 19 12 37 19 19 8 8 7 9 4 2 0 1 0 1 2 2
Nucleolus 14 7 0 2 4 5 17 7 10 15 10 10 7 4 2 6 4 1 0 0 0 1 1 2
Peroxisomes 0 3 2 1 1 0 0 0 1 1 0 0 4 12 12 15 2 3 7 5 16 14 22 23
SpindlePole 0 1 0 0 0 1 7 5 1 3 3 2 1 3 0 3 0 1 0 0 0 0 0 1
Vac/Vac Membrane 2 34 9 8 11 8 14 6 6 9 8 9 23 39 28 35 16 13 0 10 2 2 4 5
Unique Cell Count 129 252 151 99 127 113 204 206 214 362 234 273 248 354 294 134 81 58 122 255 151 175 253 245
Labelled Cell Count 157 329 180 116 152 144 271 248 255 452 286 335 298 438 365 156 97 68 122 255 151 175 253 245


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 7.7 6.1 5.9 5.6 4.9 4.0 4.6 4.3 4.0 4.2 4.0 7.8 7.8 7.9 8.8 10.2 11.0 7.1 7.1 8.1
Std Deviation (1e-4) 1.2 1.5 1.2 1.3 1.3 1.3 0.8 1.5 1.2 1.1 1.1 1.2 1.4 1.5 1.4 1.9 2.1 2.1 1.6 1.7 1.7
Intensity Change (Log2) -0.04 -0.11 -0.31 -0.61 -0.4 -0.49 -0.62 -0.53 -0.59 0.36 0.37 0.38 0.53 0.75 0.86 0.22 0.23 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 4.4 0 0
Cytoplasm 0 0 0 0 2.5 0 0 0 0 0 0 2.0 4.4 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 3.5 4.1 5.2 8.6 8.2
Mitochondria -0.5 -0.6 -1.4 0 0 0 0 0 0 -2.6 -2.9 -3.4 -15.3 -13.1 -12.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 -0.5 1.1 0 0 0 0 0 0 -1.0 0 -1.6 0.5 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 3.5 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 0.9 0.4 0.3 -1.4 -1.5 -1.9 -1.2 -1.3 1.2 1.8 1.3 4.7 3.2 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0296 0.0104 0.0102 0.0173 0.009 0.0031 0.0087 0.0062 0.0139 0.0004 0.0004 0.0042 0.0193 0.0028 0.0242 0.0085 0.0648 0.012
Bud 0.0011 0.0034 0.0008 0.0006 0 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0002 0.0003 0.0001 0.0005 0.0004 0.0011 0.0001
Bud Neck 0.0183 0.0005 0.0013 0.0003 0.0001 0.0001 0.0001 0.0002 0.0002 0 0.0001 0.0002 0.0035 0.0001 0.0002 0.0019 0.0001 0.0001
Bud Periphery 0.0025 0.0101 0.0019 0.0005 0.0003 0.0002 0.0012 0.0004 0.0004 0.0001 0.0007 0.0003 0.0005 0.0002 0.0009 0.0015 0.01 0.0002
Bud Site 0.0225 0.0101 0.015 0.0012 0 0.0001 0.0006 0.0005 0.0017 0.0001 0 0.0003 0.0043 0.0002 0.0021 0.0012 0.0003 0.0001
Cell Periphery 0.0041 0.0009 0.0055 0.0001 0.0002 0.0003 0.0003 0.0002 0.0002 0 0.0001 0.0001 0.0019 0.0001 0.0002 0.0007 0.0001 0.0001
Cytoplasm 0.0173 0.0007 0.0003 0.0004 0.0007 0 0 0.0001 0.0029 0 0.0001 0.0001 0.0064 0 0.0002 0.0001 0.0002 0
Cytoplasmic Foci 0.05 0.0139 0.0074 0.0092 0.0162 0.001 0.0003 0.008 0.0155 0.0007 0.0057 0.0055 0.0464 0.0033 0.0165 0.0032 0.0148 0.0038
Eisosomes 0.002 0.0005 0.0009 0.0001 0.0009 0.0002 0.0017 0.0003 0.0004 0.0001 0.0001 0.0002 0.0029 0.0002 0.0003 0.0003 0.0002 0.0002
Endoplasmic Reticulum 0.0132 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0028 0 0.0001 0.0001 0.0012 0 0.0001 0.0057 0.0001 0.0001
Endosome 0.0452 0.0222 0.0145 0.0472 0.001 0.0045 0.0005 0.0086 0.022 0.0043 0.0411 0.0234 0.0659 0.0079 0.0398 0.0085 0.0131 0.0124
Golgi 0.0338 0.0662 0.0229 0.0218 0.0019 0.0221 0.0008 0.0255 0.0097 0.0028 0.0702 0.0548 0.0482 0.0213 0.0268 0.0071 0.0523 0.0217
Lipid Particles 0.0199 0.0356 0.0183 0.0044 0.012 0.009 0.0048 0.024 0.0444 0.0002 0.0242 0.0162 0.059 0.0133 0.0085 0.0069 0.0036 0.0139
Mitochondria 0.5357 0.7468 0.8699 0.8745 0.8972 0.9514 0.9658 0.8859 0.8636 0.9861 0.8522 0.8643 0.6118 0.9052 0.8499 0.8349 0.8218 0.9134
None 0.0572 0.0004 0.0002 0 0.0341 0 0.0003 0.0001 0.001 0 0 0.0001 0.0635 0 0.0001 0.0003 0.0002 0
Nuclear Periphery 0.0312 0.0006 0.0003 0.0001 0.0004 0 0.0001 0.0001 0.0014 0 0.0001 0.0001 0.0006 0 0.0001 0.0147 0 0
Nucleolus 0.0015 0.0004 0.0003 0.0002 0.0002 0.0001 0.0001 0.0003 0.0004 0 0 0.0001 0.0017 0 0.0001 0.0005 0 0.0001
Nucleus 0.0053 0.0003 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0005 0 0 0.0001 0.0008 0 0.0001 0.0005 0 0
Peroxisomes 0.0733 0.0627 0.0243 0.0109 0.0185 0.006 0.0128 0.0363 0.0075 0.0038 0.0026 0.0273 0.0516 0.0429 0.0267 0.0785 0.017 0.0193
Punctate Nuclear 0.0254 0.0001 0.0001 0 0.0065 0 0 0 0.0078 0 0 0 0.005 0 0.0007 0 0.0001 0
Vacuole 0.0053 0.005 0.0025 0.0079 0.0002 0.0005 0.0006 0.0011 0.0027 0.0006 0.0008 0.0008 0.0032 0.0007 0.0009 0.0077 0.0001 0.0008
Vacuole Periphery 0.0056 0.009 0.003 0.003 0.0007 0.0013 0.0009 0.0017 0.0008 0.0006 0.0013 0.0017 0.0016 0.0016 0.001 0.0168 0.0002 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.8324 20.6301 27.2681 26.1434 21.0384 23.065 20.6961 24.4173 27.7878 25.152
Translational Efficiency 0.5589 0.5177 0.5033 0.6318 0.5976 0.5872 0.6584 0.6895 0.5917 0.5245

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2355 104 268 1607 1705 645 212 1490 4060 749 480 3097

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 793.07 1169.49 1197.71 1053.09 835.67 946.78 1298.13 990.99 810.96 977.70 1242.06 1023.21
Standard Deviation 106.53 682.25 190.62 156.65 117.62 146.91 170.60 150.28 113.29 298.58 188.76 156.72
Intensity Change Log 2 0.560359 0.594759 0.409109 0.180096 0.635430 0.245937 0.377767 0.615769 0.327695

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000650 0.006490 0.002173 0.020116 0.000667 0.004393 0.005802 0.003769 0.000657 0.004684 0.003776 0.012251
Bud Neck 0.002026 0.009284 0.006585 0.002225 0.000767 0.002197 0.004461 0.004541 0.001497 0.003181 0.005647 0.003339
Bud Site 0.006459 0.014068 0.014005 0.053230 0.001840 0.006114 0.015433 0.038592 0.004519 0.007219 0.014635 0.046187
Cell Periphery 0.000866 0.000501 0.000189 0.001127 0.000237 0.000284 0.000504 0.000792 0.000602 0.000314 0.000328 0.000966
Cytoplasm 0.001594 0.004061 0.001900 0.013331 0.000386 0.001343 0.002394 0.018913 0.001086 0.001721 0.002118 0.016016
Cytoplasmic Foci 0.011860 0.108593 0.055545 0.034245 0.015211 0.039857 0.090055 0.049814 0.013267 0.049401 0.070787 0.041736
Eisosomes 0.000044 0.000125 0.000071 0.000141 0.000034 0.000077 0.000102 0.000074 0.000040 0.000084 0.000085 0.000108
Endoplasmic Reticulum 0.000693 0.000550 0.000878 0.001054 0.000373 0.000336 0.001492 0.001393 0.000559 0.000365 0.001149 0.001217
Endosome 0.042320 0.060487 0.056674 0.046438 0.030701 0.064232 0.084733 0.078761 0.037440 0.063712 0.069066 0.061989
Golgi 0.032393 0.062254 0.067376 0.105765 0.025399 0.070020 0.137621* 0.103826 0.029456 0.068942 0.098401 0.104832
Lipid Particles 0.003414 0.010077 0.004895 0.000548 0.004278 0.005188 0.002111 0.002231 0.003777 0.005867 0.003665 0.001357
Mitochondria 0.829675* 0.621963* 0.617639* 0.618356* 0.881524* 0.743894* 0.501852* 0.537565* 0.851449* 0.726964* 0.566500* 0.579487*
Mitotic Spindle 0.002881 0.016890 0.068338 0.033322 0.002481 0.005716 0.056797 0.041010 0.002713 0.007267 0.063241 0.037021
None 0.000565 0.000245 0.000611 0.000324 0.000169 0.000200 0.000464 0.000950 0.000399 0.000207 0.000546 0.000625
Nuclear Periphery 0.000412 0.000239 0.004492 0.000169 0.000090 0.000120 0.001216 0.000502 0.000277 0.000137 0.003045 0.000329
Nuclear Periphery Foci 0.000454 0.000680 0.002552 0.000314 0.000299 0.000222 0.000968 0.001105 0.000389 0.000286 0.001852 0.000694
Nucleolus 0.000432 0.000737 0.000755 0.000119 0.000169 0.000178 0.000203 0.000468 0.000321 0.000256 0.000511 0.000287
Nucleus 0.000647 0.000523 0.000962 0.000304 0.000109 0.000236 0.000364 0.001613 0.000421 0.000276 0.000698 0.000933
Peroxisomes 0.010288 0.065262 0.029172 0.032512 0.022111 0.033895 0.027948 0.015201 0.015253 0.038251 0.028631 0.024183
Vacuole 0.022220 0.012728 0.029903 0.019028 0.004495 0.009832 0.030561 0.058207 0.014776 0.010234 0.030194 0.037877
Vacuole Periphery 0.030106 0.004241 0.035287 0.017334 0.008662 0.011664 0.034919 0.040676 0.021101 0.010634 0.035124 0.028564

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.19 -3.39 -9.77 -4.15 -8.77 -3.46 -4.08 -6.22 0.49 1.47 -4.03 -5.11 -10.83 -5.23 -6.87
Bud Neck -1.32 -3.32 -0.70 1.27 3.19 -2.76 -4.98 -6.74 -3.30 -0.17 -1.88 -4.94 -5.50 -0.26 2.62
Bud Site -1.72 -1.95 -13.74 -7.11 -7.24 -3.15 -4.45 -12.97 -10.56 -5.67 -2.00 -3.86 -18.60 -15.41 -9.05
Cell Periphery 1.56 3.27 -0.81 -2.41 -3.98 -0.89 -1.80 -1.73 -1.59 -0.82 2.30 2.00 -1.57 -3.29 -3.09
Cytoplasm -1.03 -0.55 -9.44 -3.44 -8.77 -2.21 -3.96 -9.28 -8.66 -8.07 -1.18 -2.49 -12.91 -11.56 -11.66
Cytoplasmic Foci -4.52 -6.61 -11.99 3.51 3.13 -7.19 -8.15 -12.69 -2.49 4.24 -8.52 -10.40 -17.47 1.80 5.08
Eisosomes -4.97 -3.21 -13.37 -0.90 -6.45 -7.99 -5.53 -11.51 0.47 2.23 -8.49 -6.22 -16.51 -4.01 -2.92
Endoplasmic Reticulum 0.72 -0.86 -1.85 -2.42 -0.61 0.24 -2.01 -5.34 -8.40 0.15 1.82 -2.15 -4.81 -8.21 -0.24
Endosome -1.84 -2.53 -1.28 1.42 1.79 -6.90 -7.27 -12.41 -2.60 0.80 -6.14 -6.88 -9.79 0.42 1.51
Golgi -2.65 -4.19 -15.08 -3.64 -4.09 -8.00 -9.14 -15.85 -4.72 2.64 -7.92 -9.42 -21.68 -6.09 -0.75
Lipid Particles -1.02 -0.85 5.04 1.47 2.58 -0.56 2.44 2.13 1.88 -0.22 -1.30 0.09 4.53 2.85 2.31
Mitochondria 5.87 9.43 20.00 0.11 0.06 9.65 14.54 30.23* 12.75 -1.19 9.62 16.52 34.85* 10.64 -0.57
Mitotic Spindle -2.88 -6.80 -12.55 -3.21 3.48 -3.06 -6.44 -12.17 -10.71 1.71 -4.02 -9.25 -17.36 -13.35 3.78
None 2.39 -0.33 1.86 -2.24 5.92 -1.48 -6.16 -2.01 -1.93 -1.24 2.49 -1.77 -1.12 -2.22 -0.41
Nuclear Periphery 1.28 -2.11 2.33 0.69 2.23 -1.23 -3.89 -4.72 -4.36 2.36 2.30 -2.54 -0.77 -4.02 2.49
Nuclear Periphery Foci -0.91 -3.44 0.97 1.40 3.65 0.66 -3.15 -2.58 -3.09 -0.46 1.16 -4.11 -1.95 -2.79 3.06
Nucleolus -0.80 -1.03 1.84 1.59 2.14 -0.06 -0.31 -2.18 -2.16 -1.97 0.49 -1.03 0.22 -0.33 1.23
Nucleus 0.29 -0.78 0.92 0.44 1.36 -1.04 -1.15 -3.83 -3.43 -2.92 0.83 -1.07 -2.53 -3.20 -0.99
Peroxisomes -3.64 -3.13 -7.75 2.13 -0.44 -2.54 -0.93 2.39 4.21 2.08 -5.41 -3.09 -4.56 3.17 1.01
Vacuole 1.72 -2.06 0.56 -1.45 2.30 -3.31 -6.79 -17.56 -14.29 -5.78 2.61 -5.72 -12.70 -12.53 -2.89
Vacuole Periphery 14.67 -1.07 6.22 -8.68 3.86 -1.72 -4.76 -13.70 -10.96 -0.95 6.32 -3.88 -4.52 -9.80 1.81
Standard name
Human Ortholog
Description Protein that may act as a chaperone for cytochrome c oxidase subunits; conserved protein; may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Afg1

Afg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Afg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available