Standard name | |
---|---|
Human Ortholog | |
Description | 2-hydroxyglutarate transhydrogenase, and minor D-lactate dehydrogenase; converts D-2-hydroxyglutarate (D-2HG), an oncometabolite, to alpha-ketoglutarate in the presence of FAD, with concomitant reduction of pyruvate to D-lactate; minor lactate dehydrogenase activity; component of the retrograde regulon that consists of genes whose expression are stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source; located in the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 7 | 18 | 22 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 0 | 2 | 3 | 1 | 10 | 13 | 5 | 7 | 3 | 2 | 6 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 106 | 352 | 122 | 141 | 135 | 185 | 148 | 272 | 171 | 141 | 110 | 105 | 680 | 679 | 772 | 149 | 138 | 132 | 112 | 353 | 113 | 496 | 474 | 451 |
Endoplasmic Reticulum | 0 | 2 | 0 | 1 | 2 | 4 | 4 | 15 | 8 | 5 | 8 | 6 | 6 | 4 | 5 | 5 | 4 | 3 | 0 | 0 | 2 | 2 | 2 | 2 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 7 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 8 | 18 | 12 | 12 | 12 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 7 | 7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 |
Vac/Vac Membrane | 0 | 0 | 3 | 3 | 3 | 6 | 11 | 35 | 20 | 22 | 7 | 17 | 7 | 4 | 5 | 18 | 7 | 4 | 0 | 2 | 0 | 3 | 4 | 13 |
Unique Cell Count | 106 | 355 | 125 | 144 | 139 | 196 | 166 | 329 | 217 | 185 | 140 | 139 | 694 | 687 | 783 | 173 | 144 | 137 | 117 | 373 | 119 | 524 | 527 | 514 |
Labelled Cell Count | 106 | 357 | 127 | 145 | 142 | 198 | 171 | 337 | 233 | 203 | 145 | 150 | 702 | 690 | 790 | 185 | 155 | 141 | 117 | 373 | 119 | 524 | 527 | 514 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 43.0 | 37.1 | 23.8 | 30.1 | 38.3 | 33.3 | 23.1 | 19.2 | 20.7 | 17.3 | 21.0 | 21.8 | 48.0 | 52.8 | 53.3 | 38.1 | 49.5 | 44.7 | 25.3 | 27.9 | 29.9 |
Std Deviation (1e-4) | 10.4 | 10.8 | 6.5 | 9.6 | 13.7 | 14.9 | 11.8 | 9.0 | 9.0 | 8.5 | 8.1 | 9.0 | 20.9 | 19.8 | 20.9 | 19.7 | 23.5 | 19.0 | 8.3 | 10.1 | 10.1 |
Intensity Change (Log2) | – | – | – | 0.34 | 0.69 | 0.48 | -0.04 | -0.31 | -0.2 | -0.46 | -0.18 | -0.13 | 1.01 | 1.15 | 1.16 | 0.68 | 1.06 | 0.91 | 0.09 | 0.23 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -0.2 | -1.4 | -2.8 | -4.2 | -4.8 | -5.1 | -4.7 | -5.2 | 0.3 | 1.1 | 0.8 | -3.4 | -0.8 | -0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 3.3 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 1.7 | 2.8 | 2.4 | 3.0 | 0 | 3.0 | 0 | 0 | 0 | 2.7 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 112.1085 | 154.6099 | 131.4508 | 126.2745 | 125.6057 | 102.5002 | 130.3778 | 189.3147 | 158.7831 | 166.8393 | 131.8205 | 174.2042 | 119.5306 | 170.9516 | 150.7511 | 136.6349 | 125.393 | 124.9616 |
Actin | 0.0115 | 0.0002 | 0.006 | 0.0003 | 0.0155 | 0.0041 | 0.0045 | 0.0009 | 0.0041 | 0.0045 | 0.0108 | 0.0027 | 0.0083 | 0.0001 | 0.0031 | 0.0158 | 0.0003 | 0.0004 |
Bud | 0.0006 | 0.0002 | 0.0008 | 0.0006 | 0.0004 | 0.0008 | 0.0009 | 0.0003 | 0.0012 | 0.0003 | 0.0017 | 0.0023 | 0.0019 | 0.0003 | 0.0014 | 0.0004 | 0.0005 | 0.0009 |
Bud Neck | 0.0005 | 0.0002 | 0.0007 | 0.0004 | 0.0008 | 0.0014 | 0.0004 | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0.0009 | 0.0005 | 0.0001 | 0.0002 | 0.0014 | 0.0002 | 0.0008 |
Bud Periphery | 0.0004 | 0 | 0.0005 | 0.0002 | 0.0005 | 0.0005 | 0.0004 | 0.0001 | 0.0007 | 0.0002 | 0.0021 | 0.0028 | 0.001 | 0.0003 | 0.0014 | 0.0003 | 0.0006 | 0.0004 |
Bud Site | 0.0009 | 0.0001 | 0.0077 | 0.0001 | 0.0007 | 0.0006 | 0.0007 | 0.0012 | 0.0004 | 0.0013 | 0.001 | 0.0009 | 0.0024 | 0.0001 | 0.0011 | 0.003 | 0.0001 | 0.0004 |
Cell Periphery | 0.0004 | 0.0012 | 0.0005 | 0.0001 | 0.0008 | 0.0007 | 0.0017 | 0.0003 | 0.0009 | 0.0004 | 0.0003 | 0.0006 | 0.0012 | 0.0002 | 0.0008 | 0.0003 | 0.0012 | 0.0015 |
Cytoplasm | 0.836 | 0.9809 | 0.9428 | 0.9301 | 0.8401 | 0.7138 | 0.8904 | 0.9823 | 0.9394 | 0.9192 | 0.6964 | 0.9361 | 0.8327 | 0.9862 | 0.9341 | 0.8913 | 0.8578 | 0.8891 |
Cytoplasmic Foci | 0.0295 | 0.0002 | 0.0048 | 0.0081 | 0.02 | 0.0108 | 0.0061 | 0.0004 | 0.0057 | 0.0009 | 0.0033 | 0.0016 | 0.0352 | 0.0003 | 0.0062 | 0.0142 | 0.0634 | 0.0178 |
Eisosomes | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0008 | 0.0003 | 0.0014 | 0.0023 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0024 | 0.0002 | 0.0003 | 0.0003 | 0.0027 | 0.0094 | 0.003 | 0.0001 | 0.0007 | 0.0415 | 0.0032 | 0.001 | 0.0023 | 0.0001 | 0.0006 | 0.0054 | 0.0013 | 0.0006 |
Endosome | 0.0086 | 0 | 0.0008 | 0.0001 | 0.0044 | 0.0374 | 0.0009 | 0.0001 | 0.0006 | 0.0024 | 0.024 | 0.0006 | 0.0062 | 0 | 0.0019 | 0.0047 | 0.001 | 0.0008 |
Golgi | 0.0017 | 0 | 0.0004 | 0 | 0.0027 | 0.003 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0024 | 0.0001 | 0.0054 | 0 | 0.0013 | 0.0022 | 0.0002 | 0.0001 |
Lipid Particles | 0.0064 | 0.0001 | 0.0032 | 0.0002 | 0.0055 | 0.0189 | 0.0013 | 0.0001 | 0.0013 | 0.0005 | 0.0006 | 0.0002 | 0.0078 | 0 | 0.0017 | 0.0031 | 0.0022 | 0.0034 |
Mitochondria | 0.0048 | 0.0003 | 0.0024 | 0.0031 | 0.0226 | 0.0066 | 0.0028 | 0.0048 | 0.0101 | 0.0022 | 0.062 | 0.0171 | 0.0121 | 0.0004 | 0.0114 | 0.0036 | 0.015 | 0.0046 |
None | 0.0117 | 0.0009 | 0.0071 | 0.0301 | 0.0089 | 0.0214 | 0.0104 | 0.0006 | 0.0036 | 0.0025 | 0.0087 | 0.0039 | 0.0249 | 0.0006 | 0.0081 | 0.0167 | 0.0213 | 0.0042 |
Nuclear Periphery | 0.0145 | 0.0049 | 0.0019 | 0.0034 | 0.0066 | 0.0597 | 0.0192 | 0.0004 | 0.0054 | 0.0082 | 0.0272 | 0.0032 | 0.0045 | 0.0007 | 0.0025 | 0.0075 | 0.0039 | 0.0032 |
Nucleolus | 0.0094 | 0.0007 | 0.0033 | 0.0019 | 0.0209 | 0.0053 | 0.0085 | 0.0026 | 0.0052 | 0.0008 | 0.0753 | 0.0023 | 0.0152 | 0.0012 | 0.0038 | 0.0087 | 0.0033 | 0.0366 |
Nucleus | 0.0345 | 0.0078 | 0.0093 | 0.0178 | 0.0215 | 0.0744 | 0.0385 | 0.0027 | 0.0123 | 0.0107 | 0.0439 | 0.0187 | 0.0215 | 0.006 | 0.0064 | 0.0128 | 0.009 | 0.0184 |
Peroxisomes | 0.0023 | 0 | 0.0006 | 0.0002 | 0.001 | 0.0011 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.0028 | 0 | 0.0004 | 0.0018 | 0.0008 | 0.0007 |
Punctate Nuclear | 0.0198 | 0.0001 | 0.0014 | 0.001 | 0.0012 | 0.009 | 0.0046 | 0.0001 | 0.0029 | 0.0008 | 0.0021 | 0.0007 | 0.0027 | 0 | 0.0005 | 0.0031 | 0.0034 | 0.0013 |
Vacuole | 0.0026 | 0.0014 | 0.0035 | 0.0013 | 0.0105 | 0.0113 | 0.0025 | 0.0002 | 0.0022 | 0.0017 | 0.0137 | 0.0023 | 0.0074 | 0.0024 | 0.0081 | 0.0017 | 0.0105 | 0.0108 |
Vacuole Periphery | 0.0013 | 0.0005 | 0.0016 | 0.0007 | 0.012 | 0.0095 | 0.0011 | 0.0003 | 0.0024 | 0.0012 | 0.0203 | 0.0013 | 0.0038 | 0.001 | 0.0048 | 0.002 | 0.004 | 0.004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 868.4407 | 461.7643 | 486.4831 | 512.2773 | 366.0486 | 794.3664 | 566.7221 | 524.8219 | 590.6775 | 500.2246 |
Translational Efficiency | 1.4938 | 1.4419 | 1.2699 | 1.4796 | 1.6356 | 1.8247 | 1.5155 | 1.5497 | 1.4389 | 1.4651 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2857 | 1377 | 243 | 1363 | 1457 | 2123 | 1018 | 343 | 4314 | 3500 | 1261 | 1706 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1889.46 | 2722.31 | 3132.66 | 4050.71 | 2062.73 | 2977.91 | 2986.30 | 4283.83 | 1947.98 | 2877.35 | 3014.50 | 4097.58 |
Standard Deviation | 453.18 | 812.73 | 632.68 | 1124.31 | 553.89 | 856.49 | 647.59 | 1205.52 | 496.33 | 848.78 | 647.33 | 1144.92 |
Intensity Change Log 2 | — | 0.526857 | 0.729414 | 1.100201 | — | 0.529745 | 0.533804 | 1.054346 | — | 0.528365 | 0.630634 | 1.076450 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000025 | 0.000083 | 0.000370 | 0.000492 | 0.000064 | 0.000083 | 0.000132 | 0.000384 | 0.000038 | 0.000083 | 0.000178 | 0.000470 |
Bud Neck | 0.000286 | 0.000340 | 0.005257 | 0.001869 | 0.000308 | 0.000530 | 0.000877 | 0.007738 | 0.000294 | 0.000456 | 0.001721 | 0.003049 |
Bud Site | 0.000638 | 0.000768 | 0.002792 | 0.011116 | 0.001112 | 0.001684 | 0.000757 | 0.030273 | 0.000798 | 0.001324 | 0.001149 | 0.014967 |
Cell Periphery | 0.000030 | 0.000208 | 0.000749 | 0.000620 | 0.000114 | 0.000118 | 0.000274 | 0.000757 | 0.000059 | 0.000153 | 0.000366 | 0.000648 |
Cytoplasm | 0.846647* | 0.760974* | 0.510769* | 0.705991* | 0.774316* | 0.756532* | 0.567860* | 0.721978* | 0.822218* | 0.758280* | 0.556858* | 0.709206* |
Cytoplasmic Foci | 0.002988 | 0.003161 | 0.016280 | 0.009414 | 0.002879 | 0.003265 | 0.004645 | 0.022947 | 0.002951 | 0.003224 | 0.006887 | 0.012135 |
Eisosomes | 0.000000 | 0.000001 | 0.000004 | 0.000004 | 0.000000 | 0.000000 | 0.000001 | 0.000006 | 0.000000 | 0.000001 | 0.000002 | 0.000004 |
Endoplasmic Reticulum | 0.000059 | 0.000027 | 0.000156 | 0.000027 | 0.000051 | 0.000033 | 0.000063 | 0.000114 | 0.000056 | 0.000031 | 0.000081 | 0.000044 |
Endosome | 0.000053 | 0.000038 | 0.000514 | 0.000242 | 0.000064 | 0.000120 | 0.000074 | 0.006097 | 0.000057 | 0.000088 | 0.000159 | 0.001419 |
Golgi | 0.000110 | 0.000313 | 0.000930 | 0.000562 | 0.000343 | 0.000645 | 0.000239 | 0.004522 | 0.000189 | 0.000514 | 0.000372 | 0.001358 |
Lipid Particles | 0.000053 | 0.000160 | 0.002020 | 0.001813 | 0.000080 | 0.000163 | 0.000411 | 0.002018 | 0.000062 | 0.000162 | 0.000721 | 0.001854 |
Mitochondria | 0.000079 | 0.000106 | 0.000618 | 0.000175 | 0.000104 | 0.000129 | 0.000227 | 0.000685 | 0.000087 | 0.000120 | 0.000303 | 0.000277 |
Mitotic Spindle | 0.000808 | 0.000346 | 0.004063 | 0.002866 | 0.000325 | 0.002033 | 0.001913 | 0.010615 | 0.000645 | 0.001369 | 0.002327 | 0.004424 |
None | 0.022911 | 0.024919 | 0.009470 | 0.012887 | 0.020954 | 0.022725 | 0.011682 | 0.008883 | 0.022250 | 0.023588 | 0.011256 | 0.012082 |
Nuclear Periphery | 0.000049 | 0.000049 | 0.000892 | 0.000164 | 0.000040 | 0.000084 | 0.000291 | 0.000265 | 0.000046 | 0.000070 | 0.000407 | 0.000184 |
Nuclear Periphery Foci | 0.000396 | 0.000782 | 0.002689 | 0.001672 | 0.000674 | 0.000598 | 0.000782 | 0.001689 | 0.000490 | 0.000671 | 0.001149 | 0.001675 |
Nucleolus | 0.000086 | 0.000089 | 0.000315 | 0.000171 | 0.000060 | 0.000148 | 0.000088 | 0.000342 | 0.000077 | 0.000125 | 0.000132 | 0.000205 |
Nucleus | 0.122388 | 0.203924* | 0.403654* | 0.233341* | 0.196928* | 0.208311* | 0.401268* | 0.133858* | 0.147563* | 0.206585* | 0.401728* | 0.213339* |
Peroxisomes | 0.000010 | 0.000010 | 0.000041 | 0.000071 | 0.000009 | 0.000013 | 0.000008 | 0.000172 | 0.000010 | 0.000012 | 0.000014 | 0.000091 |
Vacuole | 0.002003 | 0.003030 | 0.038159 | 0.015852 | 0.001369 | 0.002479 | 0.008352 | 0.040989 | 0.001789 | 0.002696 | 0.014096 | 0.020906 |
Vacuole Periphery | 0.000379 | 0.000671 | 0.000260 | 0.000652 | 0.000207 | 0.000304 | 0.000055 | 0.005666 | 0.000321 | 0.000448 | 0.000094 | 0.001660 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.18 | -7.92 | -2.66 | -2.17 | 2.34 | -2.36 | -8.78 | -4.80 | -3.69 | 1.60 | -2.53 | -10.24 | -3.01 | -2.51 | 0.45 |
Bud Neck | -4.41 | -10.61 | -10.94 | -9.54 | 8.56 | -3.43 | -12.68 | -5.97 | -5.53 | -3.27 | -4.96 | -14.65 | -11.80 | -10.21 | 3.73 |
Bud Site | -1.65 | -9.50 | -5.87 | -5.43 | -0.39 | -1.37 | -2.95 | -4.03 | -3.80 | -3.69 | -2.52 | -10.19 | -7.02 | -6.37 | -5.00 |
Cell Periphery | -10.16 | -11.67 | -13.03 | -8.83 | 5.20 | -1.12 | -9.91 | -8.21 | -8.03 | -2.43 | -8.71 | -16.14 | -14.74 | -12.04 | -0.32 |
Cytoplasm | 18.16 | 32.41 | 28.46 | 11.26 | -18.81 | 5.68 | 33.59 | 9.26 | 6.90 | -9.00 | 20.27 | 52.50 | 27.09 | 12.88 | -22.85 |
Cytoplasmic Foci | -3.45 | -10.26 | -9.75 | -7.61 | 6.87 | -2.32 | -9.85 | -8.19 | -7.91 | -5.39 | -3.99 | -13.64 | -12.63 | -11.37 | -0.09 |
Eisosomes | -3.43 | -11.26 | -4.80 | -4.33 | 6.36 | -2.81 | -12.99 | -8.01 | -7.59 | -1.71 | -4.33 | -16.52 | -6.26 | -5.80 | 1.94 |
Endoplasmic Reticulum | 1.91 | -5.19 | 1.90 | -0.06 | 6.74 | 2.12 | -5.14 | 0.78 | -0.55 | 5.01 | 2.08 | -5.42 | 2.25 | 0.59 | 9.48 |
Endosome | 0.20 | -7.40 | -2.38 | -2.40 | 6.30 | -1.74 | -4.00 | -1.45 | -1.36 | -1.28 | -1.89 | -9.00 | -1.94 | -1.64 | -0.07 |
Golgi | -3.49 | -9.80 | -3.02 | -0.21 | 6.57 | -1.41 | -0.34 | -1.19 | -0.67 | -1.14 | -2.96 | -7.50 | -2.30 | 0.14 | 0.61 |
Lipid Particles | -4.30 | -8.19 | -5.40 | -4.87 | 2.89 | -2.52 | -11.11 | -7.00 | -6.69 | -4.80 | -4.45 | -13.12 | -7.28 | -6.73 | -2.20 |
Mitochondria | -3.04 | -4.74 | -5.49 | -3.19 | 3.96 | -2.54 | -13.30 | -1.53 | -1.02 | 1.14 | -4.58 | -11.59 | -4.38 | -2.03 | 6.39 |
Mitotic Spindle | 1.47 | -3.18 | -2.56 | -3.27 | 1.25 | -3.98 | -4.66 | -3.54 | -1.48 | -1.47 | -2.88 | -4.77 | -4.06 | -1.75 | 0.00 |
None | 0.06 | 18.84 | 14.29 | 7.68 | -6.28 | -0.02 | 10.33 | 13.66 | 14.32 | 7.69 | 0.46 | 19.01 | 18.13 | 13.51 | 0.52 |
Nuclear Periphery | -2.55 | -12.81 | -16.54 | -15.41 | 11.16 | -8.19 | -22.51 | -13.05 | -10.83 | 2.01 | -7.88 | -22.89 | -20.77 | -15.83 | 14.28 |
Nuclear Periphery Foci | -3.87 | -7.58 | -5.52 | -2.75 | 4.32 | -0.25 | -6.85 | -6.35 | -6.32 | -2.92 | -3.60 | -11.09 | -7.12 | -5.40 | 0.97 |
Nucleolus | -0.59 | -10.13 | -1.93 | -1.41 | 7.33 | -3.12 | -11.94 | -4.26 | -0.63 | -1.57 | -2.76 | -13.18 | -3.51 | -0.26 | 2.47 |
Nucleus | -17.35 | -17.85 | -20.34 | -4.16 | 9.29 | -3.99 | -27.89 | 6.78 | 9.97 | 28.61 | -18.49 | -42.01 | -13.65 | 0.21 | 26.63 |
Peroxisomes | -0.40 | -8.04 | -3.34 | -3.17 | 2.53 | -1.83 | -5.48 | -2.65 | -2.47 | -2.27 | -1.41 | -9.05 | -4.22 | -3.94 | -2.14 |
Vacuole | -6.15 | -11.89 | -16.89 | -14.77 | 7.99 | -6.63 | -15.05 | -13.10 | -12.57 | -8.95 | -8.19 | -17.79 | -20.28 | -18.54 | -0.76 |
Vacuole Periphery | -0.39 | 0.11 | 0.43 | 0.59 | 0.59 | -0.46 | 1.15 | -0.92 | -0.82 | -1.09 | -0.33 | 1.41 | -0.37 | 0.01 | -1.46 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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