Standard name | |
---|---|
Human Ortholog | |
Description | Alpha-1,3-mannosyltransferase; integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 1 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | – | 3 | 1 | 2 | 0 | 2 | 0 | – | – | 0 | 3 | 7 | 8 | 9 | 7 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 1 | 0 | 0 | 1 | 0 | 0 | 3 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 90 | 68 | 134 | 122 | 97 | 114 | 159 | 194 | – | 259 | 291 | 297 | 93 | 196 | 212 | – | – | 54 | 11 | 10 | 12 | 19 | 20 | 36 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 3 | 1 | 2 | 1 | – | 9 | 1 | 1 | 0 | 2 | 1 | – | – | 9 | 0 | 0 | 1 | 2 | 3 | 3 |
Endosome | 35 | 0 | 45 | 6 | 16 | 0 | 3 | 0 | – | 0 | 0 | 1 | 3 | 2 | 0 | – | – | 0 | 17 | 10 | 17 | 4 | 8 | 15 |
Golgi | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 3 | 1 | 0 | 1 | 1 | 0 |
Mitochondria | 6 | 46 | 48 | 10 | 22 | 73 | 67 | 72 | – | 116 | 68 | 58 | 0 | 0 | 0 | – | – | 9 | 6 | 5 | 8 | 4 | 8 | 11 |
Nucleus | 16 | 11 | 29 | 12 | 29 | 47 | 79 | 53 | – | 33 | 17 | 19 | 7 | 6 | 1 | – | – | 55 | 3 | 14 | 3 | 2 | 3 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | – | 7 | 2 | 8 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 2 | 1 | 4 | 0 | 0 | 0 | 1 | 2 | – | 0 | 1 | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 1 | 2 | 6 | 3 | 2 | 7 | 4 |
Vac/Vac Membrane | 191 | 89 | 236 | 166 | 222 | 258 | 356 | 341 | – | 288 | 233 | 272 | 65 | 32 | 16 | – | – | 60 | 248 | 151 | 351 | 175 | 220 | 238 |
Unique Cell Count | 286 | 177 | 394 | 255 | 300 | 370 | 509 | 509 | 526 | 463 | 508 | 149 | 228 | 220 | 152 | 301 | 216 | 415 | 225 | 285 | 331 | |||
Labelled Cell Count | 347 | 217 | 500 | 318 | 389 | 494 | 671 | 664 | 718 | 614 | 659 | 168 | 241 | 233 | 189 | 301 | 216 | 415 | 225 | 285 | 331 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.0 | 4.9 | 5.5 | 6.3 | 5.5 | 5.6 | 5.5 | – | 4.9 | 5.5 | 5.3 | 5.9 | 5.5 | 5.6 | 4.8 | 4.8 | 6.3 | 6.4 | 6.5 | 6.6 |
Std Deviation (1e-4) | 1.5 | 1.5 | 1.8 | 1.6 | 1.8 | 1.9 | 2.2 | 1.9 | – | 1.7 | 1.9 | 1.7 | 1.6 | 1.4 | 1.4 | 1.4 | 1.3 | 2.3 | 2.2 | 1.8 | 2.7 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.38 | 0.18 | 0.21 | 0.19 | – | 0.02 | 0.17 | 0.13 | 0.29 | 0.19 | 0.21 | -0.01 | -0.0 | 0.38 | 0.4 | 0.43 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Cytoplasm | 3.5 | -0.5 | -0.9 | -0.9 | 1.3 | – | 4.6 | 8.4 | 7.3 | 6.0 | 12.5 | 14.9 | – | – | 0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Endosome | -4.2 | -2.8 | -6.7 | -7.2 | -7.8 | – | -7.9 | -7.5 | -7.6 | -3.4 | -4.8 | -5.2 | – | – | -4.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Mitochondria | -3.6 | -2.1 | 2.9 | 0.4 | 0.9 | – | 3.9 | 1.1 | -0.4 | -4.5 | -5.5 | -5.4 | – | – | -2.1 |
Nucleus | -1.4 | 1.1 | 2.5 | 3.7 | 1.6 | – | -0.7 | -2.4 | -2.4 | -1.1 | -2.5 | -3.8 | – | – | 8.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Vacuole | 1.3 | 3.9 | 2.8 | 3.1 | 2.2 | – | -1.6 | -2.8 | -1.9 | -3.4 | -11.1 | -12.7 | – | – | -4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7259 | 2.0561 | 2.4966 | 1.8637 | 1.6732 | 1.8539 | 3.6196 | 4.9711 | 4.3786 | 3.7667 | 3.2779 | 3.773 | 2.6595 | 2.3946 | 3.1592 | 1.9737 | 2.0945 | 2.7793 |
Actin | 0.0222 | 0.0021 | 0.0026 | 0 | 0 | 0.0098 | 0.0521 | 0.0005 | 0.0143 | 0.0056 | 0.0032 | 0.0005 | 0.0001 | 0.0002 | 0.0357 | 0.0033 | 0 | 0 |
Bud | 0.0011 | 0.0046 | 0.0005 | 0.0002 | 0.0003 | 0.0077 | 0.0037 | 0.0018 | 0.0037 | 0.0005 | 0.0005 | 0.0021 | 0.0002 | 0.0008 | 0.0003 | 0.0004 | 0.0004 | 0.0004 |
Bud Neck | 0.0026 | 0.0015 | 0.0014 | 0.0013 | 0.0008 | 0.0044 | 0.0284 | 0.0041 | 0.0054 | 0.0014 | 0.0016 | 0.0057 | 0.0014 | 0.0011 | 0.001 | 0.0013 | 0.0004 | 0.0084 |
Bud Periphery | 0.0013 | 0.0104 | 0.0008 | 0.0005 | 0.0005 | 0.0035 | 0.0038 | 0.0016 | 0.0076 | 0.0011 | 0.0015 | 0.0025 | 0.0003 | 0.0013 | 0.0005 | 0.0005 | 0.0003 | 0.0011 |
Bud Site | 0.0214 | 0.0061 | 0.0026 | 0.0008 | 0.0007 | 0.0021 | 0.0085 | 0.0065 | 0.0106 | 0.0015 | 0.0022 | 0.0042 | 0.0047 | 0.0066 | 0.0011 | 0.001 | 0.0004 | 0.0014 |
Cell Periphery | 0.0011 | 0.0035 | 0.0025 | 0.0029 | 0.0006 | 0.0029 | 0.0017 | 0.0023 | 0.0028 | 0.0017 | 0.0015 | 0.0029 | 0.0016 | 0.0036 | 0.0013 | 0.0005 | 0.0002 | 0.0022 |
Cytoplasm | 0.0533 | 0.0709 | 0.0881 | 0.0038 | 0.005 | 0.0336 | 0.0376 | 0.0771 | 0.0155 | 0.0047 | 0.0055 | 0.0303 | 0.0117 | 0.1097 | 0.0541 | 0.1879 | 0.0834 | 0.0924 |
Cytoplasmic Foci | 0.0333 | 0.0295 | 0.0225 | 0.0071 | 0.0054 | 0.0304 | 0.0417 | 0.0367 | 0.0334 | 0.0213 | 0.0297 | 0.0166 | 0.048 | 0.0726 | 0.0475 | 0.0197 | 0.0455 | 0.0217 |
Eisosomes | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0011 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 | 0.0004 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0114 | 0.0069 | 0.0117 | 0.0005 | 0.0006 | 0.003 | 0.014 | 0.0054 | 0.0062 | 0.0033 | 0.0041 | 0.0029 | 0.0022 | 0.0083 | 0.0048 | 0.003 | 0.0062 | 0.004 |
Endosome | 0.3025 | 0.166 | 0.2759 | 0.2616 | 0.2304 | 0.2197 | 0.2178 | 0.201 | 0.2946 | 0.2434 | 0.3818 | 0.2198 | 0.3072 | 0.2164 | 0.2674 | 0.1956 | 0.2644 | 0.2035 |
Golgi | 0.0163 | 0.0055 | 0.0115 | 0.0019 | 0.0032 | 0.005 | 0.0191 | 0.0082 | 0.0181 | 0.032 | 0.0078 | 0.0082 | 0.0091 | 0.0056 | 0.0281 | 0.0179 | 0.0015 | 0.0029 |
Lipid Particles | 0.0452 | 0.0344 | 0.0312 | 0.0126 | 0.0063 | 0.0197 | 0.0322 | 0.0208 | 0.0393 | 0.0301 | 0.0167 | 0.0168 | 0.065 | 0.0343 | 0.0324 | 0.018 | 0.0046 | 0.0147 |
Mitochondria | 0.006 | 0.0029 | 0.0249 | 0.0002 | 0.0003 | 0.0039 | 0.0246 | 0.0042 | 0.0232 | 0.0436 | 0.0285 | 0.0053 | 0.0096 | 0.0018 | 0.0966 | 0.0409 | 0.001 | 0.0375 |
None | 0.1477 | 0.0977 | 0.0784 | 0.0001 | 0.0002 | 0.0279 | 0.0739 | 0.0475 | 0.0219 | 0.0056 | 0.0023 | 0.0103 | 0.0049 | 0.3728 | 0.0852 | 0.1529 | 0.2384 | 0.1683 |
Nuclear Periphery | 0.0108 | 0.0083 | 0.0062 | 0.001 | 0.0013 | 0.0053 | 0.0212 | 0.0053 | 0.0185 | 0.0092 | 0.0314 | 0.0035 | 0.0043 | 0.0038 | 0.0062 | 0.0036 | 0.0034 | 0.0052 |
Nucleolus | 0.0014 | 0.005 | 0.0033 | 0.0107 | 0.0015 | 0.0053 | 0.0085 | 0.0081 | 0.0057 | 0.0043 | 0.0026 | 0.006 | 0.0086 | 0.0012 | 0.001 | 0.001 | 0.0006 | 0.0055 |
Nucleus | 0.0054 | 0.0069 | 0.0055 | 0.0024 | 0.0019 | 0.0089 | 0.0273 | 0.0113 | 0.005 | 0.0031 | 0.0058 | 0.0052 | 0.006 | 0.004 | 0.0021 | 0.0024 | 0.0038 | 0.0186 |
Peroxisomes | 0.0225 | 0.0024 | 0.0016 | 0.0007 | 0.0004 | 0.003 | 0.0136 | 0.0018 | 0.0036 | 0.0036 | 0.0021 | 0.001 | 0.0032 | 0.0061 | 0.0064 | 0.0182 | 0.0002 | 0.0005 |
Punctate Nuclear | 0.0074 | 0.0016 | 0.0008 | 0.0001 | 0.0001 | 0.0025 | 0.0117 | 0.0012 | 0.0049 | 0.0013 | 0.001 | 0.0007 | 0.011 | 0.0039 | 0.0006 | 0.0003 | 0.0003 | 0.001 |
Vacuole | 0.2584 | 0.4731 | 0.3664 | 0.6328 | 0.6692 | 0.5295 | 0.2924 | 0.47 | 0.3736 | 0.5035 | 0.4142 | 0.5615 | 0.4231 | 0.1366 | 0.2816 | 0.2845 | 0.3044 | 0.3633 |
Vacuole Periphery | 0.0284 | 0.0605 | 0.0617 | 0.0586 | 0.0713 | 0.0716 | 0.0652 | 0.0844 | 0.092 | 0.079 | 0.0558 | 0.0941 | 0.0777 | 0.0094 | 0.0456 | 0.0471 | 0.0408 | 0.0472 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 70.8608 | 18.9605 | 5.7557 | 13.1439 | 72.8118 | 35.6517 | 9.5855 | 13.3802 | 11.1289 | 63.4293 |
Translational Efficiency | 1.1089 | 1.0277 | 0.8944 | 0.6985 | 0.9448 | 1.3325 | 1.0827 | 0.3386 | 0.4309 | 0.7628 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
376 | 525 | 178 | 1396 | 1508 | 1505 | 2482 | 1483 | 1884 | 2030 | 2660 | 2879 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 894.85 | 845.69 | 878.65 | 855.52 | 719.72 | 824.86 | 833.76 | 826.34 | 754.67 | 830.25 | 836.76 | 840.49 |
Standard Deviation | 122.19 | 158.73 | 91.07 | 125.40 | 113.96 | 111.67 | 98.66 | 122.73 | 135.18 | 125.88 | 98.81 | 124.89 |
Intensity Change Log 2 | — | -0.081517 | -0.026357 | -0.064844 | — | 0.196714 | 0.212196 | 0.199300 | — | 0.049173 | 0.084878 | 0.058908 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002111 | 0.000115 | 0.000481 | 0.003246 | 0.000053 | 0.000060 | 0.000127 | 0.002164 | 0.000464 | 0.000074 | 0.000150 | 0.002688 |
Bud Neck | 0.006446 | 0.022860 | 0.011982 | 0.024504 | 0.026976 | 0.023366 | 0.053393 | 0.024130 | 0.022878 | 0.023235 | 0.050622 | 0.024311 |
Bud Site | 0.048708 | 0.027715 | 0.016870 | 0.074760 | 0.024101 | 0.012483 | 0.016209 | 0.050366 | 0.029012 | 0.016423 | 0.016253 | 0.062195 |
Cell Periphery | 0.000244 | 0.000915 | 0.000239 | 0.000214 | 0.000999 | 0.001366 | 0.000134 | 0.000204 | 0.000849 | 0.001250 | 0.000141 | 0.000209 |
Cytoplasm | 0.261253* | 0.062796 | 0.167969* | 0.361717* | 0.055205 | 0.033261 | 0.196519* | 0.343824* | 0.096327 | 0.040899 | 0.194608* | 0.352500* |
Cytoplasmic Foci | 0.084366 | 0.026984 | 0.043826 | 0.052595 | 0.026048 | 0.017967 | 0.031400 | 0.073988 | 0.037687 | 0.020299 | 0.032231 | 0.063615 |
Eisosomes | 0.000087 | 0.000030 | 0.000121 | 0.000072 | 0.000029 | 0.000044 | 0.000046 | 0.000070 | 0.000041 | 0.000040 | 0.000051 | 0.000071 |
Endoplasmic Reticulum | 0.002098 | 0.001901 | 0.008170 | 0.002151 | 0.001873 | 0.004932 | 0.001734 | 0.001059 | 0.001918 | 0.004148 | 0.002165 | 0.001588 |
Endosome | 0.050024 | 0.114452 | 0.043236 | 0.041484 | 0.115228 | 0.098323 | 0.093177 | 0.081939 | 0.102215 | 0.102494 | 0.089835 | 0.062323 |
Golgi | 0.024468 | 0.003673 | 0.000868 | 0.005314 | 0.003587 | 0.003259 | 0.000852 | 0.003875 | 0.007754 | 0.003366 | 0.000853 | 0.004573 |
Lipid Particles | 0.003050 | 0.001013 | 0.009073 | 0.004952 | 0.002203 | 0.003263 | 0.003632 | 0.003545 | 0.002372 | 0.002681 | 0.003996 | 0.004227 |
Mitochondria | 0.003902 | 0.001139 | 0.000340 | 0.006885 | 0.002511 | 0.008117 | 0.001912 | 0.005939 | 0.002789 | 0.006312 | 0.001806 | 0.006398 |
Mitotic Spindle | 0.018485 | 0.001413 | 0.002028 | 0.031779 | 0.000541 | 0.000708 | 0.008896 | 0.021741 | 0.004122 | 0.000890 | 0.008437 | 0.026609 |
None | 0.000577 | 0.000862 | 0.002050 | 0.002106 | 0.000947 | 0.000811 | 0.001397 | 0.003343 | 0.000873 | 0.000824 | 0.001441 | 0.002743 |
Nuclear Periphery | 0.000676 | 0.000229 | 0.002883 | 0.001051 | 0.000279 | 0.000628 | 0.001609 | 0.001055 | 0.000358 | 0.000525 | 0.001695 | 0.001053 |
Nuclear Periphery Foci | 0.001512 | 0.000298 | 0.010896 | 0.002190 | 0.000204 | 0.000357 | 0.001135 | 0.002273 | 0.000465 | 0.000342 | 0.001788 | 0.002233 |
Nucleolus | 0.000496 | 0.000717 | 0.002741 | 0.000932 | 0.000882 | 0.001540 | 0.006008 | 0.001067 | 0.000805 | 0.001328 | 0.005789 | 0.001002 |
Nucleus | 0.045706 | 0.009836 | 0.299447* | 0.083703 | 0.009576 | 0.014186 | 0.119642 | 0.063572 | 0.016786 | 0.013061 | 0.131674* | 0.073333 |
Peroxisomes | 0.005134 | 0.002895 | 0.006732 | 0.013916 | 0.001846 | 0.001978 | 0.004285 | 0.013854 | 0.002502 | 0.002215 | 0.004449 | 0.013884 |
Vacuole | 0.435517* | 0.671455* | 0.368849* | 0.284527* | 0.685535* | 0.745036* | 0.454858* | 0.297341* | 0.635638* | 0.726006* | 0.449102* | 0.291128* |
Vacuole Periphery | 0.005139 | 0.048702 | 0.001200 | 0.001903 | 0.041377 | 0.028313 | 0.003035 | 0.004652 | 0.034145 | 0.033586 | 0.002912 | 0.003319 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.03 | 1.24 | -0.72 | -4.21 | -3.03 | -0.27 | -2.42 | -2.18 | -2.17 | -2.05 | 1.91 | 1.23 | -3.36 | -4.21 | -3.97 |
Bud Neck | -5.96 | -2.38 | -9.17 | 1.31 | -1.72 | 1.31 | -8.91 | 3.05 | 1.71 | 13.25 | -0.23 | -10.68 | 1.88 | 2.27 | 14.04 |
Bud Site | 0.88 | 2.32 | -1.07 | -2.71 | -4.16 | 5.51 | 4.12 | -2.33 | -6.37 | -5.38 | 4.37 | 4.91 | -3.61 | -7.90 | -8.46 |
Cell Periphery | -4.36 | -2.31 | 0.49 | 4.50 | 3.68 | -1.46 | 7.00 | 6.75 | 5.47 | -0.40 | -2.11 | 6.96 | 6.80 | 6.44 | -0.54 |
Cytoplasm | 12.75 | 4.93 | -4.53 | -26.28 | -11.23 | 4.85 | -23.74 | -32.95 | -36.87 | -14.23 | 10.62 | -16.08 | -33.88 | -48.04 | -18.09 |
Cytoplasmic Foci | 6.15 | 3.63 | 4.43 | -3.40 | 0.07 | 3.34 | -2.70 | -11.15 | -14.62 | -10.13 | 5.72 | 0.40 | -6.95 | -14.15 | -8.77 |
Eisosomes | 6.00 | -4.82 | 1.02 | -6.51 | 5.80 | -2.92 | -6.35 | -6.99 | -3.05 | -3.73 | -0.61 | -5.12 | -7.57 | -5.37 | -4.03 |
Endoplasmic Reticulum | -0.17 | -6.65 | -1.24 | -1.08 | 6.47 | -7.30 | 0.54 | 3.78 | 9.23 | 4.42 | -6.96 | -2.21 | 1.02 | 7.85 | 3.92 |
Endosome | -8.92 | -0.53 | 2.28 | 10.85 | 2.17 | 2.68 | 5.10 | 11.33 | 8.46 | 7.58 | -0.70 | 3.10 | 14.44 | 14.93 | 12.75 |
Golgi | 2.34 | 3.20 | 2.80 | 1.26 | -2.00 | 0.46 | 6.17 | 2.45 | 1.81 | -2.65 | 2.14 | 5.52 | 3.66 | 2.16 | -3.21 |
Lipid Particles | 4.20 | -4.76 | -2.49 | -6.97 | 3.67 | -1.87 | -3.28 | -2.30 | -0.07 | 0.99 | -0.89 | -4.32 | -3.40 | -2.30 | 1.15 |
Mitochondria | 1.55 | 2.83 | -0.76 | -2.07 | -3.00 | -4.34 | 1.43 | -0.87 | 3.38 | -1.79 | -3.95 | 1.74 | -1.02 | 2.94 | -2.28 |
Mitotic Spindle | 3.01 | 2.86 | -2.70 | -7.77 | -7.67 | -0.06 | -6.64 | -8.07 | -8.17 | -3.65 | 2.52 | -4.23 | -9.67 | -11.61 | -5.94 |
None | -0.45 | -2.06 | -7.87 | -2.17 | -0.57 | 0.45 | -1.51 | -3.51 | -4.14 | -2.92 | 0.17 | -2.25 | -5.15 | -5.35 | -3.91 |
Nuclear Periphery | 5.73 | -5.93 | -7.20 | -15.23 | 2.90 | -2.35 | -10.80 | -10.58 | -5.59 | 1.98 | -0.90 | -10.94 | -15.97 | -10.06 | 1.67 |
Nuclear Periphery Foci | 7.07 | -3.41 | -1.82 | -11.07 | 3.24 | -1.04 | -4.93 | -5.56 | -4.83 | -1.62 | 1.39 | -5.24 | -8.15 | -8.12 | -0.30 |
Nucleolus | -1.26 | -2.80 | -2.74 | -1.60 | 2.07 | -2.87 | -9.67 | -2.53 | 1.34 | 9.13 | -2.93 | -9.95 | -2.73 | 1.01 | 9.38 |
Nucleus | 7.62 | -11.08 | -9.42 | -22.51 | 8.35 | -2.53 | -29.53 | -21.01 | -19.08 | 6.71 | 2.60 | -28.61 | -26.71 | -29.05 | 7.04 |
Peroxisomes | 0.51 | -0.60 | -2.55 | -2.98 | -0.74 | -0.21 | -5.35 | -6.40 | -6.25 | -4.17 | -0.00 | -4.57 | -7.19 | -7.02 | -4.44 |
Vacuole | -7.52 | 4.83 | 6.88 | 18.77 | -0.07 | -5.44 | 23.71 | 29.68 | 35.38* | 8.45 | -7.65 | 21.86 | 30.51 | 40.83* | 8.64 |
Vacuole Periphery | -11.16 | 1.81 | 2.03 | 12.01 | 1.07 | 5.13 | 18.45 | 18.13 | 16.87 | 0.49 | 0.07 | 17.98 | 18.42 | 20.98 | 2.66 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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