Standard name
Human Ortholog
Description Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0.06 0 0.05 0.09 – 0.07 0.09 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.93 0.86 0.88 0.8 0.77 – 0.77 0.77 0.74 0.73 0.96 0.98 0.99 0.95 0.92 0.96 0.94 0.95 0.9 0.89 0.92 0.91
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.06 0.05
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.06 – 0.16 0.18 0.27 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.14 0.09 0.15 0.14 – 0.13 0.05 0.08 0.07 0 0 0 0 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 2 3 0 5 – 6 6 4 3 0 0 0 0 0 0 0 0 4 0 1 0
Bud 0 0 0 0 0 2 0 – 7 0 3 5 0 0 0 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 4 16 17 7 11 37 – 26 40 30 35 2 11 9 5 4 3 0 0 0 0 0 0
Cytoplasm 304 237 205 243 214 170 313 – 299 342 265 325 149 350 317 129 138 212 292 227 212 162 266 248
Endoplasmic Reticulum 6 5 10 3 4 1 2 – 3 15 3 11 7 9 4 4 2 10 10 3 8 8 16 12
Endosome 0 0 0 0 0 1 3 – 1 6 4 3 0 0 0 0 0 0 1 0 1 1 0 0
Golgi 0 0 1 1 8 1 11 – 1 0 1 0 0 0 0 0 2 0 0 0 1 0 0 2
Mitochondria 0 0 1 4 2 28 25 – 62 80 97 103 0 0 0 2 4 3 1 0 1 0 0 1
Nucleus 0 0 0 1 0 3 3 – 4 1 3 1 0 1 0 1 1 3 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 2 1 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 7 39 21 31 58 – 49 20 28 33 0 0 1 2 13 4 2 2 0 3 1 0
Unique Cell Count 310 240 221 282 242 212 406 389 444 358 443 156 357 321 136 150 220 312 239 236 182 290 273
Labelled Cell Count 317 246 240 310 259 250 459 459 510 438 520 159 371 331 143 164 236 312 239 236 182 290 273


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.1 18.3 17.2 17.3 15.8 10.7 11.6 – 9.7 9.1 8.7 8.5 21.1 23.5 23.5 21.0 19.7 21.4 16.9 18.5 19.6
Std Deviation (1e-4) 3.5 3.6 3.5 3.2 2.6 3.8 2.4 – 2.4 1.7 2.4 1.6 5.5 4.9 5.0 4.9 6.0 5.8 3.0 3.7 4.3
Intensity Change (Log2) – – – 0.0 -0.12 -0.69 -0.57 – -0.83 -0.92 -0.98 -1.03 0.29 0.45 0.45 0.28 0.19 0.31 -0.03 0.1 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 -2.2 -0.9 0.8 – -0.3 0.8 0.5 0.3 -2.7 -2.3 -2.4 -1.4 -1.9 -3.0
Cytoplasm -2.4 -1.6 -3.8 -4.9 – -5.0 -5.0 -5.6 -5.9 1.1 3.2 3.6 0.8 -0.3 1.7
Endoplasmic Reticulum -2.4 -1.8 -2.7 0 – 0 -0.7 0 -1.4 0 -1.3 -2.4 -0.7 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.3 3.4 – 6.0 6.5 8.3 7.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 4.1 2.5 4.2 4.4 – 3.9 0.8 2.3 2.2 0 0 0 0 2.3 -0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.8714 12.9744 10.3952 11.0302 7.9349 11.0604 9.6736 14.3405 13.6351 10.3307 12.4469 12.8658 15.3381 18.7007 16.8509 16.8875 15.3282 16.8986
Actin 0.0098 0.0007 0.0055 0.0002 0.044 0.001 0.0109 0.0005 0.0036 0.0027 0.0002 0.0069 0.0036 0.0014 0.001 0.0008 0.0032 0.0019
Bud 0.0014 0.0011 0.0009 0.0005 0.0113 0.0007 0.003 0.0024 0.0004 0.0021 0.0006 0.0008 0.0004 0.0003 0.0004 0.0007 0.0004 0.0003
Bud Neck 0.0006 0.0003 0.0009 0.0007 0.0045 0.0011 0.0012 0.0003 0.0018 0.0021 0.0004 0.0017 0.0005 0.0003 0.0005 0.0011 0.0003 0.0013
Bud Periphery 0.0008 0.0003 0.0007 0.0001 0.0029 0.0003 0.0073 0.003 0.0002 0.003 0.0003 0.0007 0.0004 0.0002 0.0003 0.0004 0.0002 0.0007
Bud Site 0.0014 0.0013 0.0013 0.0003 0.0037 0.0001 0.0061 0.0012 0.0008 0.0068 0.0007 0.0014 0.0004 0.0004 0.0008 0.0004 0.0002 0.0001
Cell Periphery 0.0008 0.0003 0.0005 0.0001 0.0002 0.0001 0.0013 0.0004 0.0004 0.0007 0.0001 0.0002 0.0013 0.0003 0.0013 0.0004 0.0001 0.0004
Cytoplasm 0.7771 0.9453 0.8516 0.865 0.6802 0.8843 0.6999 0.9176 0.8808 0.6408 0.8332 0.8386 0.8471 0.9374 0.921 0.8841 0.8621 0.8954
Cytoplasmic Foci 0.0203 0.0056 0.0186 0.0117 0.0333 0.0129 0.0326 0.0044 0.0235 0.0201 0.0148 0.0127 0.0061 0.0036 0.008 0.0091 0.0123 0.0082
Eisosomes 0.0001 0 0.0002 0 0.0002 0 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0763 0.0131 0.0223 0.0079 0.0176 0.0417 0.0704 0.0076 0.0132 0.0286 0.0063 0.0388 0.0831 0.0319 0.0223 0.0112 0.0214 0.0623
Endosome 0.0505 0.013 0.0443 0.0533 0.0902 0.0378 0.0605 0.0056 0.0378 0.1127 0.0905 0.0295 0.0158 0.0103 0.0229 0.0417 0.0452 0.0158
Golgi 0.0079 0.0011 0.0093 0.0021 0.0145 0.0059 0.01 0.0006 0.0043 0.006 0.0069 0.0117 0.0025 0.0028 0.0022 0.0012 0.0099 0.0022
Lipid Particles 0.0041 0.0001 0.004 0.0001 0.0256 0.0001 0.0132 0.0002 0.0019 0.004 0.0009 0.0048 0.0016 0.0002 0.0005 0.0002 0.0063 0.0011
Mitochondria 0.0107 0.0005 0.0054 0.0009 0.0048 0.0055 0.0062 0.0002 0.0017 0.0458 0.0013 0.0194 0.0011 0.0007 0.0006 0.0004 0.0068 0.0013
None 0.0031 0.001 0.0078 0.0006 0.0032 0.0006 0.0108 0.0255 0.0028 0.0272 0.0004 0.0026 0.0027 0.0003 0.0015 0.0005 0.0004 0.0006
Nuclear Periphery 0.0046 0.004 0.0028 0.0024 0.0043 0.0012 0.0125 0.0026 0.0027 0.0059 0.0025 0.0093 0.005 0.0016 0.0019 0.0036 0.0049 0.0015
Nucleolus 0.0002 0 0.0003 0 0.0006 0 0.0006 0.0003 0.0001 0.0009 0 0.0004 0.0001 0 0 0 0 0
Nucleus 0.0026 0.001 0.0014 0.0016 0.0027 0.0008 0.0042 0.0106 0.0015 0.0027 0.0014 0.0075 0.0021 0.001 0.0012 0.0019 0.0018 0.0011
Peroxisomes 0.0036 0 0.0024 0.0001 0.014 0.0001 0.0139 0.0001 0.0018 0.0013 0.0004 0.0039 0.002 0 0.0001 0.0001 0.0017 0.0007
Punctate Nuclear 0.0039 0.0001 0.0014 0.0002 0.0018 0.0001 0.0042 0.0094 0.0006 0.0039 0.0001 0.0024 0.003 0.0001 0.0001 0.0001 0.0002 0.0007
Vacuole 0.0167 0.0092 0.0155 0.0437 0.0346 0.004 0.0263 0.0067 0.0175 0.0751 0.0308 0.0052 0.0194 0.0065 0.0118 0.0363 0.0182 0.0035
Vacuole Periphery 0.0035 0.0019 0.003 0.0084 0.0058 0.0016 0.0049 0.0008 0.0024 0.0074 0.0081 0.0014 0.0019 0.0008 0.0015 0.0059 0.0044 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 101.0444 99.3602 91.1566 109.5708 75.9849 88.8698 100.0064 134.3787 102.6428 114.2092
Translational Efficiency 2.2107 2.3773 2.4632 1.8738 2.391 2.9781 2.7608 1.6869 2.362 2.3448

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
290 1356 287 1889 1531 2328 2576 1701 1821 3684 2863 3590

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1202.19 1571.43 2308.44 2155.02 1554.87 1501.29 2107.84 2285.96 1498.70 1527.11 2127.95 2217.06
Standard Deviation 237.07 285.81 339.17 455.56 272.41 249.03 332.79 463.20 296.63 265.33 338.83 463.82
Intensity Change Log 2 — 0.386413 0.941253 0.842036 — -0.050591 0.438971 0.556006 — 0.156386 0.679701 0.687747

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000494 0.000202 0.000893 0.000250 0.000152 0.000113 0.000097 0.000187 0.000207 0.000146 0.000177 0.000220
Bud Neck 0.000939 0.000276 0.008038 0.001072 0.000205 0.000203 0.000347 0.000677 0.000322 0.000230 0.001118 0.000885
Bud Site 0.072336 0.014926 0.027536 0.015646 0.010295 0.010654 0.004271 0.008203 0.020175 0.012226 0.006603 0.012119
Cell Periphery 0.004300 0.001068 0.006930 0.007922 0.000794 0.000199 0.001941 0.005285 0.001352 0.000519 0.002441 0.006673
Cytoplasm 0.518450* 0.889687* 0.607052* 0.858102* 0.856984* 0.896712* 0.947897* 0.894412* 0.803071* 0.894126* 0.913729* 0.875306*
Cytoplasmic Foci 0.260597* 0.043786 0.042894 0.007044 0.064284 0.049581 0.003353 0.006098 0.095547 0.047448 0.007317 0.006596
Eisosomes 0.000013 0.000002 0.000007 0.000002 0.000001 0.000002 0.000001 0.000002 0.000003 0.000002 0.000001 0.000002
Endoplasmic Reticulum 0.011186 0.003723 0.001761 0.000523 0.006025 0.002056 0.000313 0.000436 0.006847 0.002670 0.000458 0.000482
Endosome 0.012929 0.002848 0.011658 0.001116 0.003848 0.002191 0.000418 0.000773 0.005294 0.002433 0.001544 0.000954
Golgi 0.036783 0.015360 0.056641 0.009093 0.022641 0.009908 0.004896 0.008717 0.024893 0.011915 0.010083 0.008915
Lipid Particles 0.001943 0.002723 0.020837 0.008892 0.001392 0.000554 0.004311 0.006396 0.001480 0.001352 0.005967 0.007709
Mitochondria 0.001446 0.000106 0.000071 0.000117 0.000101 0.000478 0.000057 0.000094 0.000315 0.000341 0.000058 0.000106
Mitotic Spindle 0.001588 0.001593 0.014123 0.000632 0.001231 0.001228 0.000285 0.000342 0.001288 0.001362 0.001672 0.000494
None 0.000636 0.000433 0.000344 0.000273 0.000264 0.000413 0.000551 0.000348 0.000323 0.000420 0.000530 0.000308
Nuclear Periphery 0.000074 0.000021 0.000418 0.000040 0.000031 0.000013 0.000022 0.000089 0.000038 0.000016 0.000062 0.000063
Nuclear Periphery Foci 0.001446 0.001756 0.016689 0.001860 0.001689 0.000325 0.001565 0.001786 0.001650 0.000852 0.003081 0.001825
Nucleolus 0.000108 0.000024 0.000083 0.000091 0.000017 0.000026 0.000034 0.000070 0.000031 0.000025 0.000039 0.000081
Nucleus 0.004314 0.001460 0.009723 0.003199 0.000970 0.001658 0.003833 0.003834 0.001503 0.001585 0.004423 0.003500
Peroxisomes 0.000623 0.000140 0.000152 0.000220 0.000074 0.000170 0.000054 0.000119 0.000161 0.000159 0.000064 0.000172
Vacuole 0.017854 0.011243 0.149553 0.074434 0.010152 0.013693 0.022598 0.054751 0.011378 0.012791 0.035325 0.065108
Vacuole Periphery 0.051942 0.008627 0.024599 0.009472 0.018852 0.009824 0.003158 0.007383 0.024122 0.009383 0.005307 0.008482

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.00 -3.27 2.34 -2.09 6.44 1.66 2.37 -2.08 -4.04 -5.20 2.34 0.72 -1.32 -5.00 -2.71
Bud Neck 3.22 -8.04 -1.11 -7.64 7.89 0.28 -2.76 -8.06 -8.48 -5.14 2.21 -7.31 -8.24 -10.72 2.11
Bud Site 11.48 7.68 11.26 -0.63 4.53 -0.35 6.64 1.75 2.37 -6.23 6.14 10.65 5.79 -0.29 -6.95
Cell Periphery 2.29 -1.53 -2.16 -9.85 -0.98 7.12 -5.72 -7.93 -9.10 -5.62 3.45 -3.59 -10.42 -13.71 -8.67
Cytoplasm -20.22 -3.03 -18.28 5.21 -14.03 -5.92 -14.86 -4.69 1.15 11.72 -13.22 -15.37 -9.65 5.25 8.70
Cytoplasmic Foci 16.91 16.67 20.07 16.33 12.01 4.25 21.15 19.77 20.59 -4.37 12.46 24.56 24.81 26.18 1.44
Eisosomes 3.60 1.82 3.45 -1.75 6.71 -2.41 3.91 -4.24 -1.94 -6.45 2.67 3.12 1.67 -3.14 -4.92
Endoplasmic Reticulum 3.02 3.97 4.48 4.76 4.13 4.63 7.10 6.85 4.83 -0.73 5.00 8.23 8.18 6.82 -0.22
Endosome 7.69 0.68 9.16 5.56 10.21 5.33 12.10 10.38 8.08 -2.95 8.25 10.79 13.09 9.11 3.89
Golgi 8.13 -3.42 10.51 5.11 10.30 9.17 14.32 10.14 1.28 -4.13 10.72 12.22 13.19 3.83 1.32
Lipid Particles -1.70 -9.76 -8.64 -7.52 5.90 6.05 -7.30 -8.78 -10.43 -3.01 0.69 -10.73 -12.74 -12.91 -2.63
Mitochondria 2.25 2.30 2.23 -0.51 -2.43 -2.91 2.82 0.22 2.91 -1.24 -0.20 2.66 2.15 2.84 -2.85
Mitotic Spindle -0.18 -4.37 2.96 2.49 4.65 0.02 2.90 2.68 2.00 -0.82 -0.24 -1.45 2.74 2.78 3.84
None 0.43 0.61 0.77 2.77 2.03 -1.87 -7.58 -2.57 0.79 5.08 -1.01 -2.42 0.20 2.06 6.81
Nuclear Periphery 1.47 -6.11 0.90 -3.88 8.28 1.68 0.78 -1.18 -1.59 -1.38 2.04 -2.24 -1.03 -2.12 0.05
Nuclear Periphery Foci -0.37 -9.64 -0.59 -0.76 10.76 9.31 0.53 -0.48 -9.41 -1.01 4.38 -5.44 -0.99 -8.07 5.48
Nucleolus 0.95 0.28 0.25 -3.08 -0.30 0.36 0.04 -1.48 -10.24 -8.96 0.67 0.31 -1.46 -7.34 -12.24
Nucleus 1.06 -2.43 0.16 -10.35 6.60 -6.28 -15.98 -13.98 -10.65 -1.46 -0.19 -6.85 -5.80 -14.48 2.26
Peroxisomes 4.06 5.66 5.01 -0.27 -2.04 -4.30 1.84 -2.21 2.56 -4.99 -0.40 5.57 -0.17 0.32 -8.44
Vacuole 1.87 -11.34 -12.97 -19.39 6.45 -2.72 -8.60 -15.94 -14.58 -11.31 -1.24 -12.58 -24.14 -24.14 -11.50
Vacuole Periphery 7.22 4.50 7.16 -0.49 5.13 5.02 9.42 6.30 2.18 -3.99 8.26 10.91 8.97 1.35 -4.20
Standard name
Human Ortholog
Description Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Tma20

Tma20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tma20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available