Standard name
Human Ortholog
Description Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.3 0.42 0.27 0.34 0.17 0.18 – 0.18 0.19 0.14 0.2 0.17 0.39 0.47 0.38 0.24 0.26 0.25 0.29 0.27 0.34 0.14 0.21 0.22
Bud 0.16 0.25 0.19 0.29 0.22 0.17 – 0.18 0.18 0.17 0.19 0.15 0.15 0.17 0.16 0.19 0.15 0.14 0.32 0.36 0.3 0.43 0.47 0.38
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0 0.15 0.18 0.16 0.24 0.13 0.14
Bud Site 0.05 0.13 0.18 0.13 0.1 0.12 – 0.06 0.09 0.07 0.07 0.06 0.06 0 0 0.13 0.09 0.11 – – – – – –
Cell Periphery 0.23 0.07 0.08 0 0.07 0.08 – 0.08 0.14 0.09 0.14 0.14 0.16 0.19 0.2 0.1 0.05 0.08 0.07 0 0 0 0 0
Cytoplasm 0.3 0.27 0.2 0.19 0.29 0.3 – 0.23 0.15 0.16 0.19 0.15 0.41 0.34 0.44 0.29 0.38 0.32 0.09 0.07 0.05 0.06 0.1 0.13
Endoplasmic Reticulum 0.07 0 0 0 0 0 – 0 0 0 0.05 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0.08 0.05 0.12 0.05 – 0.06 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.06 0.07 0.06 0.21 – 0.34 0.48 0.53 0.53 0.55 0 0 0 0.09 0.09 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.06 0 0 0 0 – 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.14 0.06 0.23 0.23 0.18 0.15 – 0.12 0.11 0.13 0.08 0.12 0 0 0.05 0.14 0.12 0.16 0 0 0 0 0 0
SpindlePole 0.14 0.05 0.22 0.16 0.11 0.07 – 0.09 0.07 0.07 0 0.05 0.13 0.09 0.07 0.21 0.22 0.24 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 121 90 93 62 61 – 81 96 78 82 84 96 92 89 137 103 89 22 95 126 44 113 103
Bud 7 71 65 78 79 59 – 82 90 98 77 73 36 33 36 109 61 50 24 123 110 139 252 180
Bud Neck 1 3 6 6 11 10 – 7 15 9 9 9 1 0 1 13 33 5 11 63 60 78 69 64
Bud Site 2 36 60 35 35 42 – 28 45 38 29 30 14 6 2 73 34 40 – – – – – –
Cell Periphery 10 19 28 12 25 26 – 35 70 52 58 67 38 37 46 55 19 29 5 8 12 0 6 7
Cytoplasm 13 77 67 52 103 103 – 104 76 94 76 76 101 66 101 164 150 114 7 23 18 18 50 59
Endoplasmic Reticulum 3 9 5 7 8 13 – 17 18 14 19 12 14 7 7 17 12 17 1 7 6 5 8 10
Endosome 1 11 28 14 44 16 – 28 16 25 8 16 4 4 6 25 23 25 0 4 8 7 10 9
Golgi 1 3 1 0 1 0 – 0 0 0 0 0 2 2 0 0 0 1 0 2 4 2 0 2
Mitochondria 1 19 19 18 21 70 – 155 244 301 215 271 1 1 0 52 36 23 0 4 3 4 3 8
Nucleus 1 2 7 2 6 2 – 5 4 4 3 2 1 1 0 20 9 5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 2 0 0 – 1 0 2 0 0 0 1 0 0 0 0 0 0 2 1 1 2
Nucleolus 1 16 12 4 15 15 – 23 18 30 13 19 0 0 0 14 7 9 0 1 0 0 0 0
Peroxisomes 6 16 77 62 64 52 – 56 58 75 33 59 10 6 12 81 47 58 0 2 5 3 3 3
SpindlePole 6 13 74 42 39 25 – 43 37 41 13 23 31 18 16 119 87 85 1 6 11 7 5 7
Vac/Vac Membrane 0 0 5 5 11 2 – 14 11 15 6 9 2 5 1 3 8 10 1 0 1 4 4 4
Unique Cell Count 43 285 338 270 356 338 455 507 570 408 495 244 197 232 571 400 360 78 346 375 324 535 470
Labelled Cell Count 66 416 544 432 524 496 679 798 876 641 750 351 279 317 882 629 560 78 346 375 324 535 470


Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 9.2 7.4 7.3 6.9 6.2 – 6.0 5.7 5.4 5.3 5.4 8.8 9.1 9.1 7.0 7.5 8.9 9.3 9.2 9.6
Std Deviation (1e-4) 1.6 2.0 1.5 1.6 1.7 1.7 – 1.4 1.3 1.4 1.2 1.4 1.1 1.5 1.5 1.4 1.5 1.8 1.4 1.3 1.4
Intensity Change (Log2) – – – -0.02 -0.1 -0.25 – -0.31 -0.39 -0.45 -0.5 -0.45 0.24 0.29 0.3 -0.09 0.01 0.27 0.32 0.31 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.1 -2.9 -2.7 – -3.0 -2.6 -4.9 -2.1 -3.4 3.2 4.7 3.0 -0.9 -0.3 -0.6
Bud 2.8 1.0 -0.6 – -0.4 -0.5 -0.8 -0.1 -1.7 -1.4 -0.7 -1.1 -0.1 -1.4 -1.9
Bud Neck 0.4 1.1 1.0 – -0.3 1.1 -0.2 0.4 0 0 0 0 0.5 3.9 -0.4
Bud Site -1.6 -3.0 -1.9 – -5.1 -3.8 -5.2 -4.5 -5.3 -4.3 -5.0 -6.4 -2.0 -3.8 -2.5
Cell Periphery -1.9 -0.6 -0.3 – -0.3 2.5 0.4 2.5 2.3 2.7 3.6 4.0 0.7 -2.0 -0.1
Cytoplasm -0.2 2.8 3.2 – 1.0 -1.8 -1.3 -0.4 -1.7 5.7 3.5 6.1 3.0 5.3 3.6
Endoplasmic Reticulum 1.0 0.7 1.9 – 1.9 1.8 1.0 2.4 0.9 2.9 0 0 1.4 1.4 2.5
Endosome -1.5 1.8 -1.9 – -1.2 -3.3 -2.4 -4.0 -3.2 -3.5 -2.9 -2.8 -2.4 -1.4 -0.7
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.2 5.8 – 9.6 13.1 14.4 13.8 14.6 -3.4 -3.0 -3.7 1.9 1.7 0.4
Nucleus 0 -0.4 0 – -1.1 0 0 0 0 0 0 0 1.2 0.2 -0.7
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.6 0.5 0.6 – 1.0 0 1.2 -0.3 0.2 -3.0 0 0 -1.0 -1.5 -0.8
Peroxisomes 0.1 -1.6 -2.4 – -3.9 -4.4 -3.8 -5.6 -4.2 -6.2 -6.1 -5.7 -3.3 -4.0 -2.2
SpindlePole -2.0 -3.9 -5.3 – -4.9 -6.2 -6.4 -7.9 -7.6 -2.8 -3.8 -4.8 -0.4 0 0.5
Vacuole 0 1.4 0 – 1.5 0.7 1.1 0 0.4 0 0 0 0 0.5 1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7996 5.9003 6.0526 5.9275 5.6333 6.3521 6.3371 5.8604 6.1672 5.3351 6.187 6.3999 7.122 6.1356 6.9487 7.1797 7.4949 7.364
Actin 0.1716 0.0887 0.1232 0.1637 0.4469 0.1491 0.2452 0.0761 0.1026 0.2332 0.3997 0.1962 0.2134 0.0721 0.1535 0.3576 0.3635 0.1202
Bud 0.0631 0.0922 0.2747 0.1122 0.045 0.0145 0.0582 0.1134 0.3046 0.0872 0.052 0.0201 0.0348 0.0702 0.2234 0.0982 0.068 0.0195
Bud Neck 0.0859 0.0072 0.0157 0.0745 0.0766 0.6335 0.055 0.0046 0.0178 0.0552 0.0557 0.6081 0.1067 0.0098 0.0268 0.0068 0.063 0.6229
Bud Periphery 0.1008 0.0797 0.3665 0.4217 0.14 0.0339 0.0835 0.0971 0.3715 0.3481 0.2106 0.043 0.0838 0.0553 0.3408 0.3311 0.098 0.0338
Bud Site 0.3359 0.4738 0.1003 0.1351 0.046 0.0301 0.3242 0.494 0.1196 0.0974 0.0784 0.0311 0.2559 0.5219 0.1416 0.0533 0.127 0.028
Cell Periphery 0.0193 0.0059 0.0029 0.0104 0.0032 0.0033 0.022 0.0059 0.002 0.0025 0.0111 0.0065 0.0297 0.0103 0.0043 0.0123 0.0044 0.0054
Cytoplasm 0.0914 0.1717 0.0431 0.0097 0.096 0.0179 0.0758 0.1389 0.0264 0.1157 0.0755 0.0262 0.1047 0.1924 0.0674 0.0379 0.0506 0.0455
Cytoplasmic Foci 0.0524 0.0192 0.0147 0.0316 0.0239 0.0568 0.0572 0.0235 0.0131 0.0389 0.048 0.033 0.0481 0.0226 0.0109 0.014 0.0589 0.0801
Eisosomes 0.0023 0.0009 0.0004 0.0002 0.0009 0.0003 0.0013 0.0003 0.0005 0.0002 0.0004 0.0005 0.0021 0.0003 0.0004 0.0017 0.0012 0.0004
Endoplasmic Reticulum 0.0014 0.0025 0.0009 0.0003 0.0033 0.0003 0.0033 0.001 0.0013 0.0015 0.0008 0.0007 0.0048 0.0023 0.0013 0.0022 0.0015 0.0005
Endosome 0.008 0.0082 0.0066 0.0047 0.0308 0.0056 0.0178 0.0025 0.0068 0.003 0.0206 0.0034 0.0168 0.003 0.0033 0.0054 0.0015 0.0027
Golgi 0.0077 0.0062 0.0033 0.0045 0.0151 0.0109 0.0106 0.0019 0.0063 0.0043 0.0105 0.0043 0.0065 0.0018 0.0024 0.005 0.0038 0.0066
Lipid Particles 0.0046 0.0009 0.0016 0.0011 0.0056 0.0025 0.0051 0.0006 0.0019 0.0006 0.0075 0.0011 0.0033 0.0006 0.0012 0.0098 0.0021 0.002
Mitochondria 0.0042 0.0019 0.0046 0.0024 0.0087 0.0047 0.0061 0.0112 0.01 0.0015 0.002 0.0009 0.0019 0.0007 0.0012 0.0027 0.0306 0.0008
None 0.0136 0.0191 0.0188 0.0021 0.0048 0.0006 0.0034 0.0191 0.0074 0.0023 0.0014 0.0025 0.0438 0.017 0.0106 0.0185 0.064 0.0162
Nuclear Periphery 0.0008 0.001 0.005 0.0002 0.0032 0.0001 0.0041 0.0006 0.0007 0.0004 0.0009 0.0019 0.009 0.0004 0.0005 0.0008 0.0003 0.0001
Nucleolus 0.0022 0.0011 0.0004 0.0007 0.0004 0.003 0.0005 0.0003 0.0002 0.0001 0.0027 0.0003 0.0021 0.0013 0.0016 0.0004 0.0091 0.0004
Nucleus 0.0023 0.003 0.0014 0.0008 0.0017 0.0015 0.002 0.0012 0.0005 0.0005 0.0012 0.0012 0.0101 0.0049 0.0012 0.0006 0.0027 0.0005
Peroxisomes 0.0213 0.007 0.0086 0.0208 0.0349 0.0236 0.0201 0.0026 0.0042 0.0042 0.0077 0.0151 0.0045 0.0068 0.0022 0.0364 0.0437 0.0117
Punctate Nuclear 0.0056 0.0029 0.0045 0.0007 0.0056 0.0051 0.0019 0.0031 0.0015 0.0016 0.0098 0.0033 0.0034 0.003 0.0016 0.0012 0.0022 0.0017
Vacuole 0.0053 0.0064 0.0024 0.0025 0.0057 0.0023 0.0024 0.0017 0.0008 0.0014 0.0033 0.0005 0.0135 0.003 0.0033 0.0035 0.0036 0.001
Vacuole Periphery 0.0005 0.0005 0.0005 0.0002 0.0016 0.0003 0.0006 0.0003 0.0003 0.0002 0.0002 0.0001 0.001 0.0002 0.0003 0.0007 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.0076 16.7224 17.3358 22.3916 24.2537 19.246 21.881 19.8028 21.1556 25.2142
Translational Efficiency 0.9412 1.1192 0.9263 0.882 0.709 0.8188 0.747 0.7368 0.758 0.7015

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
Localization
Cell Percentages actin (14%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec3

Sec3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available