Standard name
Human Ortholog
Description DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0.06 0.05 0.07 0.05 0 0.05 0 0.05 0.14 0.15 0.15 0.15 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.3 0.5 0.58 0.33 0.63 0.64 0.59 0.8 0.76 0.74 0.82 0 0 0.09 0.07 0.16 0.07 0 0 0 0 0 0
Nucleus 0.93 0.86 0.89 0.87 0.86 0.77 0.78 0.74 0.69 0.74 0.74 0.76 0.95 0.86 0.83 0.79 0.79 0.72 0.88 0.94 0.83 0.85 0.78 0.74
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.13 0.05 0 0.1 0.05 0.12 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 1 0 0 1 5 6 9 5 1 7 0 0 0 0 0 0 6 0 1 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2 0 0 5 13 18 10 23 4 18 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Cytoplasm 11 0 4 7 16 17 26 43 19 21 12 10 5 32 34 33 13 25 1 0 1 2 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 3 1 5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 0 1 0 2 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0
Mitochondria 6 13 60 88 103 186 365 355 316 406 167 321 2 1 20 15 14 11 2 0 0 1 1 3
Nucleus 291 37 108 132 270 229 446 440 274 394 166 297 105 198 186 175 68 117 277 66 104 69 157 163
Nuclear Periphery 1 0 0 0 1 0 4 7 2 9 5 9 0 1 1 1 0 0 2 0 0 0 0 2
Nucleolus 1 2 0 0 1 0 2 6 3 12 3 10 0 1 1 0 0 1 2 0 0 0 3 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 4 0 0 1 2 1 0 1 0 0 0 2 1 0 0 1 3 2
Vac/Vac Membrane 7 0 3 2 7 4 11 4 3 3 1 1 2 2 3 17 5 21 15 1 12 4 24 35
Unique Cell Count 312 43 121 152 314 297 571 597 396 536 225 393 110 231 223 222 86 162 317 71 126 82 201 221
Labelled Cell Count 319 52 178 229 398 442 876 880 636 875 361 674 114 238 245 248 103 186 317 71 126 82 201 221


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.5 3.7 3.7 4.0 3.7 3.4 3.5 3.1 3.1 3.4 2.9 5.8 6.0 4.8 6.9 6.8 7.9 5.8 5.3 5.8
Std Deviation (1e-4) 0.9 1.0 0.4 1.0 1.0 1.1 1.0 1.1 1.0 1.2 1.5 0.8 1.0 1.8 1.2 1.8 1.5 2.1 2.8 1.3 1.3
Intensity Change (Log2) – – – -0.0 0.12 -0.01 -0.11 -0.06 -0.25 -0.26 -0.1 -0.36 0.66 0.69 0.39 0.9 0.88 1.09 0.66 0.53 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 2.0 – 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2882 1.7992 1.5137 1.2955 1.2152 1.769 2.9937 2.7578 2.5902 2.0913 2.5721 2.6895 1.0248 1.0018 0.5561 0.67 0.1096 0.5534
Actin 0.0001 0 0 0 0 0 0.0212 0.0109 0.0036 0.0029 0.0045 0.0124 0.0196 0 0.0046 0.0024 0 0.001
Bud 0 0 0 0 0 0 0.0015 0.0001 0.0068 0.0002 0.0008 0.0018 0.0006 0 0.0003 0.0003 0 0.0001
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0004 0.0011 0.0021 0.0001 0.0002 0.0008 0.0006 0.0026 0.0035 0 0.0007 0.0018 0.0002 0.0039
Bud Periphery 0 0 0 0 0 0.0001 0.0036 0.0001 0.0041 0.0004 0.0053 0.003 0.0013 0 0.001 0.0012 0 0.0002
Bud Site 0 0 0 0 0 0 0.0066 0.0021 0.0012 0.0008 0.0017 0.0012 0.0059 0 0.0053 0.0011 0 0.005
Cell Periphery 0 0 0 0 0 0 0.0005 0 0.0001 0.0002 0.0003 0.0002 0.0005 0 0.0015 0.0004 0 0.0001
Cytoplasm 0.001 0.002 0.0016 0.0004 0.003 0.0002 0.0081 0.0009 0.0006 0.0003 0.0002 0.0015 0.0056 0.0006 0.0087 0.0036 0.0003 0.0002
Cytoplasmic Foci 0 0 0 0 0 0 0.0201 0.0042 0.0037 0.002 0.0099 0.0011 0.0354 0 0.0075 0.0017 0 0.0009
Eisosomes 0.0002 0 0 0 0 0 0.0003 0.0001 0 0.0001 0.0001 0 0.0006 0 0.0004 0.0005 0 0
Endoplasmic Reticulum 0.0001 0.0003 0.0001 0 0.0001 0 0.009 0.0001 0.0001 0.0004 0.0006 0.0028 0.0023 0.0001 0.0061 0.0014 0 0
Endosome 0.0001 0.0002 0.0001 0 0.0002 0 0.0441 0.0017 0.0032 0.028 0.0089 0.006 0.0173 0 0.0038 0.0062 0 0.0004
Golgi 0 0 0 0 0 0 0.0204 0.0027 0.0044 0.0167 0.0108 0.0023 0.0052 0 0.0012 0.0009 0 0.0005
Lipid Particles 0 0 0 0 0 0 0.0189 0.0038 0.001 0.0043 0.0123 0.0004 0.0195 0 0.0043 0.0025 0 0.0036
Mitochondria 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0208 0.0019 0.0031 0.0266 0.0376 0.0042 0.0164 0.0001 0.0036 0.012 0.0001 0.0005
None 0.0147 0.0004 0.0027 0.0001 0.0043 0.0001 0.0046 0.0001 0.0002 0.0001 0.0002 0.0002 0.017 0.0001 0.0081 0.0171 0.0001 0.0001
Nuclear Periphery 0.005 0.0135 0.0119 0.0032 0.009 0.0016 0.0521 0.0019 0.0016 0.0012 0.005 0.0014 0.0263 0.0135 0.0071 0.009 0.0011 0.0012
Nucleolus 0.0059 0.0041 0.0044 0.0032 0.0126 0.0152 0.005 0.0033 0.0029 0.0024 0.0067 0.0123 0.0054 0.0031 0.0033 0.0059 0.0075 0.0149
Nucleus 0.9662 0.9767 0.9759 0.9921 0.955 0.9794 0.7278 0.9593 0.957 0.9059 0.8798 0.9421 0.7593 0.9818 0.9143 0.9126 0.9895 0.9527
Peroxisomes 0 0 0 0 0 0 0.0085 0.0026 0.0055 0.0007 0.0078 0.0004 0.0142 0 0.0042 0.0006 0 0.0053
Punctate Nuclear 0.0062 0.0023 0.0028 0.0008 0.0146 0.0018 0.0077 0.0037 0.0003 0.0002 0.0007 0.0006 0.0402 0.0004 0.0122 0.0115 0.0009 0.0087
Vacuole 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0109 0.0001 0.0003 0.0037 0.0026 0.0028 0.0023 0.0001 0.0015 0.0049 0 0.0005
Vacuole Periphery 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.006 0.0002 0.0002 0.0022 0.0036 0.0008 0.0016 0.0001 0.0004 0.0022 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.0236 9.7547 9.5803 12.932 10.611 7.0569 11.4585 12.5306 11.0377 12.4389
Translational Efficiency 1.0066 1.0312 0.9668 0.809 0.8974 1.0989 0.9505 0.7159 0.8184 0.7002

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
89 947 134 1082 1184 701 108 389 1273 1648 242 1471

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 768.41 767.85 1080.88 935.51 733.26 762.88 1050.76 928.33 735.72 765.74 1067.44 933.61
Standard Deviation 105.33 115.43 131.87 136.01 97.51 108.80 121.81 139.41 98.49 112.69 128.35 136.95
Intensity Change Log 2 — -0.001052 0.492258 0.283877 — 0.057131 0.519036 0.340313 — 0.027652 0.505396 0.311710

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000467 0.000552 0.001618 0.001529 0.000194 0.000424 0.000783 0.000837 0.000213 0.000497 0.001246 0.001346
Bud Neck 0.000377 0.004538 0.008911 0.024533 0.002714 0.008871 0.004231 0.012353 0.002550 0.006381 0.006822 0.021312
Bud Site 0.000805 0.001065 0.001535 0.008595 0.000452 0.001428 0.000714 0.001367 0.000476 0.001220 0.001168 0.006683
Cell Periphery 0.000148 0.000122 0.000090 0.000201 0.000079 0.000188 0.000054 0.000106 0.000083 0.000150 0.000074 0.000176
Cytoplasm 0.042806 0.016662 0.004788 0.014288 0.015732 0.033038 0.006945 0.009009 0.017624 0.023628 0.005751 0.012892
Cytoplasmic Foci 0.014028 0.003197 0.000091 0.003731 0.000996 0.009630 0.000170 0.000040 0.001907 0.005934 0.000126 0.002755
Eisosomes 0.000055 0.000025 0.000054 0.000039 0.000013 0.000035 0.000038 0.000021 0.000016 0.000029 0.000047 0.000034
Endoplasmic Reticulum 0.002926 0.006841 0.011079 0.009296 0.003075 0.004955 0.008969 0.007742 0.003065 0.006039 0.010137 0.008885
Endosome 0.001115 0.001383 0.000450 0.007907 0.000231 0.003404 0.000269 0.000228 0.000293 0.002242 0.000369 0.005877
Golgi 0.000837 0.000861 0.000112 0.003386 0.000324 0.002382 0.000042 0.000057 0.000360 0.001508 0.000081 0.002506
Lipid Particles 0.003002 0.000875 0.000352 0.002026 0.000585 0.002861 0.000269 0.000100 0.000754 0.001720 0.000315 0.001516
Mitochondria 0.000875 0.003181 0.001235 0.006150 0.000751 0.009454 0.000724 0.001463 0.000760 0.005849 0.001007 0.004911
Mitotic Spindle 0.000283 0.002746 0.000327 0.024546 0.001053 0.010532 0.000068 0.002494 0.000999 0.006058 0.000212 0.018714
None 0.015279 0.007792 0.000944 0.008438 0.005424 0.007494 0.001281 0.006821 0.006113 0.007665 0.001094 0.008011
Nuclear Periphery 0.000425 0.001299 0.000714 0.004373 0.001600 0.003751 0.000592 0.000987 0.001518 0.002342 0.000659 0.003478
Nuclear Periphery Foci 0.001913 0.000659 0.000340 0.001560 0.000629 0.000716 0.000057 0.000180 0.000719 0.000683 0.000213 0.001195
Nucleolus 0.009975 0.007703 0.009275 0.005528 0.006169 0.009722 0.001975 0.002075 0.006435 0.008562 0.006017 0.004615
Nucleus 0.900847* 0.935046* 0.952728* 0.856127* 0.958868* 0.882079* 0.968805* 0.951618* 0.954811* 0.912516* 0.959903* 0.881379*
Peroxisomes 0.001040 0.001939 0.000467 0.000934 0.000241 0.001869 0.000191 0.000100 0.000297 0.001909 0.000343 0.000713
Vacuole 0.002483 0.002253 0.004711 0.013511 0.000644 0.005406 0.003742 0.002295 0.000773 0.003594 0.004279 0.010545
Vacuole Periphery 0.000314 0.001261 0.000180 0.003305 0.000227 0.001759 0.000082 0.000107 0.000233 0.001473 0.000136 0.002460

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.10 -4.21 -2.98 -4.25 1.89 -1.53 -6.41 -8.12 -3.77 0.06 -2.83 -9.36 -8.80 -5.40 1.34
Bud Neck -4.84 -9.97 -16.31 -11.78 -7.56 -4.30 -3.44 -9.63 -3.92 -5.56 -4.44 -7.35 -16.22 -11.59 -9.68
Bud Site -0.02 -1.37 -5.22 -6.15 -5.00 -3.58 -3.19 -6.21 -1.81 -2.79 -3.75 -4.96 -7.56 -6.20 -5.58
Cell Periphery 1.47 2.11 0.76 -1.21 -2.42 -2.35 0.59 -2.02 1.47 -2.62 -2.73 -0.24 -3.54 0.48 -3.80
Cytoplasm 2.92 4.36 3.50 1.88 -3.28 -4.63 3.24 3.14 6.92 -0.72 -2.53 4.97 2.83 5.98 -3.49
Cytoplasmic Foci 2.26 2.74 2.55 2.14 -2.61 -4.38 2.96 3.43 4.90 1.71 -3.38 3.76 1.80 4.90 -2.50
Eisosomes 2.85 0.73 1.72 -5.72 2.76 -5.52 -6.90 -7.86 1.02 3.16 -5.26 -9.70 -9.19 -3.56 3.47
Endoplasmic Reticulum -4.23 -6.89 -7.95 -5.36 1.48 -4.08 -7.84 -8.28 -4.38 2.09 -8.27 -11.35 -15.88 -7.46 2.02
Endosome 0.70 1.62 -0.98 -2.24 -3.55 -4.71 -2.43 -3.05 4.51 0.40 -5.05 -1.18 -3.76 -0.46 -3.58
Golgi 0.22 1.36 -0.35 -0.69 -2.06 -2.20 0.91 0.80 2.57 -2.22 -2.23 1.01 -1.27 0.79 -2.11
Lipid Particles 2.82 3.25 2.60 -0.21 -1.40 -3.58 1.01 1.83 4.51 2.49 -2.64 1.83 0.19 2.59 -1.29
Mitochondria -2.97 -1.66 -5.42 -1.21 -5.06 -3.77 -1.53 -4.62 3.18 -3.92 -4.74 -2.77 -6.57 1.84 -5.77
Mitotic Spindle -1.91 -0.52 -6.85 -5.84 -6.81 -3.27 1.10 -1.86 1.91 -2.55 -3.26 0.92 -6.68 -4.47 -7.12
None 1.27 2.56 1.64 1.10 -6.54 -1.33 4.39 -0.10 0.96 -2.83 -1.16 5.58 0.22 1.47 -6.76
Nuclear Periphery -4.36 -2.70 -7.59 -5.56 -6.84 -3.26 1.60 -0.15 3.88 -3.83 -1.80 1.69 -3.67 -2.58 -7.27
Nuclear Periphery Foci 1.43 1.71 1.30 -0.80 -3.12 -0.41 1.16 0.85 2.84 -1.33 0.04 1.39 0.03 -0.02 -3.64
Nucleolus 1.55 0.74 2.78 2.83 1.81 -2.91 4.26 4.20 5.89 -0.46 -2.24 0.25 2.80 5.48 1.41
Nucleus -2.18 -2.82 0.60 7.48 9.15 8.63 -0.33 3.56 -5.95 3.58 7.43 0.13 10.73 3.08 10.64
Peroxisomes 0.33 1.58 1.64 2.14 0.57 -4.77 0.37 2.31 5.08 2.21 -3.58 0.05 0.20 3.66 0.22
Vacuole 0.52 -2.91 -7.51 -8.08 -5.45 -4.96 -6.16 -4.02 -0.83 -0.93 -6.11 -7.90 -9.77 -7.36 -5.70
Vacuole Periphery -1.72 0.96 -2.23 -0.18 -2.72 -2.27 1.10 0.85 2.45 -1.46 -2.95 0.77 -2.43 0.81 -2.80
Standard name
Human Ortholog
Description DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Prp22

Prp22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available