Standard name
Human Ortholog
Description DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0.06 0.05 0.07 0.05 0 0.05 0 0.05 0.14 0.15 0.15 0.15 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.3 0.5 0.58 0.33 0.63 0.64 0.59 0.8 0.76 0.74 0.82 0 0 0.09 0.07 0.16 0.07 0 0 0 0 0 0
Nucleus 0.93 0.86 0.89 0.87 0.86 0.77 0.78 0.74 0.69 0.74 0.74 0.76 0.95 0.86 0.83 0.79 0.79 0.72 0.88 0.94 0.83 0.85 0.78 0.74
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.13 0.05 0 0.1 0.05 0.12 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 1 0 0 1 5 6 9 5 1 7 0 0 0 0 0 0 6 0 1 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2 0 0 5 13 18 10 23 4 18 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Cytoplasm 11 0 4 7 16 17 26 43 19 21 12 10 5 32 34 33 13 25 1 0 1 2 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 3 1 5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 0 1 0 2 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0
Mitochondria 6 13 60 88 103 186 365 355 316 406 167 321 2 1 20 15 14 11 2 0 0 1 1 3
Nucleus 291 37 108 132 270 229 446 440 274 394 166 297 105 198 186 175 68 117 277 66 104 69 157 163
Nuclear Periphery 1 0 0 0 1 0 4 7 2 9 5 9 0 1 1 1 0 0 2 0 0 0 0 2
Nucleolus 1 2 0 0 1 0 2 6 3 12 3 10 0 1 1 0 0 1 2 0 0 0 3 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 4 0 0 1 2 1 0 1 0 0 0 2 1 0 0 1 3 2
Vac/Vac Membrane 7 0 3 2 7 4 11 4 3 3 1 1 2 2 3 17 5 21 15 1 12 4 24 35
Unique Cell Count 312 43 121 152 314 297 571 597 396 536 225 393 110 231 223 222 86 162 317 71 126 82 201 221
Labelled Cell Count 319 52 178 229 398 442 876 880 636 875 361 674 114 238 245 248 103 186 317 71 126 82 201 221


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.5 3.7 3.7 4.0 3.7 3.4 3.5 3.1 3.1 3.4 2.9 5.8 6.0 4.8 6.9 6.8 7.9 5.8 5.3 5.8
Std Deviation (1e-4) 0.9 1.0 0.4 1.0 1.0 1.1 1.0 1.1 1.0 1.2 1.5 0.8 1.0 1.8 1.2 1.8 1.5 2.1 2.8 1.3 1.3
Intensity Change (Log2) – – – -0.0 0.12 -0.01 -0.11 -0.06 -0.25 -0.26 -0.1 -0.36 0.66 0.69 0.39 0.9 0.88 1.09 0.66 0.53 0.66

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 2.0 – 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2882 1.7992 1.5137 1.2955 1.2152 1.769 2.9937 2.7578 2.5902 2.0913 2.5721 2.6895 1.0248 1.0018 0.5561 0.67 0.1096 0.5534
Actin 0.0001 0 0 0 0 0 0.0212 0.0109 0.0036 0.0029 0.0045 0.0124 0.0196 0 0.0046 0.0024 0 0.001
Bud 0 0 0 0 0 0 0.0015 0.0001 0.0068 0.0002 0.0008 0.0018 0.0006 0 0.0003 0.0003 0 0.0001
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0004 0.0011 0.0021 0.0001 0.0002 0.0008 0.0006 0.0026 0.0035 0 0.0007 0.0018 0.0002 0.0039
Bud Periphery 0 0 0 0 0 0.0001 0.0036 0.0001 0.0041 0.0004 0.0053 0.003 0.0013 0 0.001 0.0012 0 0.0002
Bud Site 0 0 0 0 0 0 0.0066 0.0021 0.0012 0.0008 0.0017 0.0012 0.0059 0 0.0053 0.0011 0 0.005
Cell Periphery 0 0 0 0 0 0 0.0005 0 0.0001 0.0002 0.0003 0.0002 0.0005 0 0.0015 0.0004 0 0.0001
Cytoplasm 0.001 0.002 0.0016 0.0004 0.003 0.0002 0.0081 0.0009 0.0006 0.0003 0.0002 0.0015 0.0056 0.0006 0.0087 0.0036 0.0003 0.0002
Cytoplasmic Foci 0 0 0 0 0 0 0.0201 0.0042 0.0037 0.002 0.0099 0.0011 0.0354 0 0.0075 0.0017 0 0.0009
Eisosomes 0.0002 0 0 0 0 0 0.0003 0.0001 0 0.0001 0.0001 0 0.0006 0 0.0004 0.0005 0 0
Endoplasmic Reticulum 0.0001 0.0003 0.0001 0 0.0001 0 0.009 0.0001 0.0001 0.0004 0.0006 0.0028 0.0023 0.0001 0.0061 0.0014 0 0
Endosome 0.0001 0.0002 0.0001 0 0.0002 0 0.0441 0.0017 0.0032 0.028 0.0089 0.006 0.0173 0 0.0038 0.0062 0 0.0004
Golgi 0 0 0 0 0 0 0.0204 0.0027 0.0044 0.0167 0.0108 0.0023 0.0052 0 0.0012 0.0009 0 0.0005
Lipid Particles 0 0 0 0 0 0 0.0189 0.0038 0.001 0.0043 0.0123 0.0004 0.0195 0 0.0043 0.0025 0 0.0036
Mitochondria 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0208 0.0019 0.0031 0.0266 0.0376 0.0042 0.0164 0.0001 0.0036 0.012 0.0001 0.0005
None 0.0147 0.0004 0.0027 0.0001 0.0043 0.0001 0.0046 0.0001 0.0002 0.0001 0.0002 0.0002 0.017 0.0001 0.0081 0.0171 0.0001 0.0001
Nuclear Periphery 0.005 0.0135 0.0119 0.0032 0.009 0.0016 0.0521 0.0019 0.0016 0.0012 0.005 0.0014 0.0263 0.0135 0.0071 0.009 0.0011 0.0012
Nucleolus 0.0059 0.0041 0.0044 0.0032 0.0126 0.0152 0.005 0.0033 0.0029 0.0024 0.0067 0.0123 0.0054 0.0031 0.0033 0.0059 0.0075 0.0149
Nucleus 0.9662 0.9767 0.9759 0.9921 0.955 0.9794 0.7278 0.9593 0.957 0.9059 0.8798 0.9421 0.7593 0.9818 0.9143 0.9126 0.9895 0.9527
Peroxisomes 0 0 0 0 0 0 0.0085 0.0026 0.0055 0.0007 0.0078 0.0004 0.0142 0 0.0042 0.0006 0 0.0053
Punctate Nuclear 0.0062 0.0023 0.0028 0.0008 0.0146 0.0018 0.0077 0.0037 0.0003 0.0002 0.0007 0.0006 0.0402 0.0004 0.0122 0.0115 0.0009 0.0087
Vacuole 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0109 0.0001 0.0003 0.0037 0.0026 0.0028 0.0023 0.0001 0.0015 0.0049 0 0.0005
Vacuole Periphery 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.006 0.0002 0.0002 0.0022 0.0036 0.0008 0.0016 0.0001 0.0004 0.0022 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.0236 9.7547 9.5803 12.932 10.611 7.0569 11.4585 12.5306 11.0377 12.4389
Translational Efficiency 1.0066 1.0312 0.9668 0.809 0.8974 1.0989 0.9505 0.7159 0.8184 0.7002

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
89 947 134 1082 1184 701 108 389 1273 1648 242 1471

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 768.41 767.85 1080.88 935.51 733.26 762.88 1050.76 928.33 735.72 765.74 1067.44 933.61
Standard Deviation 105.33 115.43 131.87 136.01 97.51 108.80 121.81 139.41 98.49 112.69 128.35 136.95
Intensity Change Log 2 — -0.001052 0.492258 0.283877 — 0.057131 0.519036 0.340313 — 0.027652 0.505396 0.311710

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000467 0.000552 0.001618 0.001529 0.000194 0.000424 0.000783 0.000837 0.000213 0.000497 0.001246 0.001346
Bud Neck 0.000377 0.004538 0.008911 0.024533 0.002714 0.008871 0.004231 0.012353 0.002550 0.006381 0.006822 0.021312
Bud Site 0.000805 0.001065 0.001535 0.008595 0.000452 0.001428 0.000714 0.001367 0.000476 0.001220 0.001168 0.006683
Cell Periphery 0.000148 0.000122 0.000090 0.000201 0.000079 0.000188 0.000054 0.000106 0.000083 0.000150 0.000074 0.000176
Cytoplasm 0.042806 0.016662 0.004788 0.014288 0.015732 0.033038 0.006945 0.009009 0.017624 0.023628 0.005751 0.012892
Cytoplasmic Foci 0.014028 0.003197 0.000091 0.003731 0.000996 0.009630 0.000170 0.000040 0.001907 0.005934 0.000126 0.002755
Eisosomes 0.000055 0.000025 0.000054 0.000039 0.000013 0.000035 0.000038 0.000021 0.000016 0.000029 0.000047 0.000034
Endoplasmic Reticulum 0.002926 0.006841 0.011079 0.009296 0.003075 0.004955 0.008969 0.007742 0.003065 0.006039 0.010137 0.008885
Endosome 0.001115 0.001383 0.000450 0.007907 0.000231 0.003404 0.000269 0.000228 0.000293 0.002242 0.000369 0.005877
Golgi 0.000837 0.000861 0.000112 0.003386 0.000324 0.002382 0.000042 0.000057 0.000360 0.001508 0.000081 0.002506
Lipid Particles 0.003002 0.000875 0.000352 0.002026 0.000585 0.002861 0.000269 0.000100 0.000754 0.001720 0.000315 0.001516
Mitochondria 0.000875 0.003181 0.001235 0.006150 0.000751 0.009454 0.000724 0.001463 0.000760 0.005849 0.001007 0.004911
Mitotic Spindle 0.000283 0.002746 0.000327 0.024546 0.001053 0.010532 0.000068 0.002494 0.000999 0.006058 0.000212 0.018714
None 0.015279 0.007792 0.000944 0.008438 0.005424 0.007494 0.001281 0.006821 0.006113 0.007665 0.001094 0.008011
Nuclear Periphery 0.000425 0.001299 0.000714 0.004373 0.001600 0.003751 0.000592 0.000987 0.001518 0.002342 0.000659 0.003478
Nuclear Periphery Foci 0.001913 0.000659 0.000340 0.001560 0.000629 0.000716 0.000057 0.000180 0.000719 0.000683 0.000213 0.001195
Nucleolus 0.009975 0.007703 0.009275 0.005528 0.006169 0.009722 0.001975 0.002075 0.006435 0.008562 0.006017 0.004615
Nucleus 0.900847 0.935046 0.952728 0.856127 0.958868 0.882079 0.968805 0.951618 0.954811 0.912516 0.959903 0.881379
Peroxisomes 0.001040 0.001939 0.000467 0.000934 0.000241 0.001869 0.000191 0.000100 0.000297 0.001909 0.000343 0.000713
Vacuole 0.002483 0.002253 0.004711 0.013511 0.000644 0.005406 0.003742 0.002295 0.000773 0.003594 0.004279 0.010545
Vacuole Periphery 0.000314 0.001261 0.000180 0.003305 0.000227 0.001759 0.000082 0.000107 0.000233 0.001473 0.000136 0.002460

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.10 -4.21 -2.98 -4.25 1.89 -1.53 -6.41 -8.12 -3.77 0.06 -2.83 -9.36 -8.80 -5.40 1.34
Bud Neck -4.84 -9.97 -16.31 -11.78 -7.56 -4.30 -3.44 -9.63 -3.92 -5.56 -4.44 -7.35 -16.22 -11.59 -9.68
Bud Site -0.02 -1.37 -5.22 -6.15 -5.00 -3.58 -3.19 -6.21 -1.81 -2.79 -3.75 -4.96 -7.56 -6.20 -5.58
Cell Periphery 1.47 2.11 0.76 -1.21 -2.42 -2.35 0.59 -2.02 1.47 -2.62 -2.73 -0.24 -3.54 0.48 -3.80
Cytoplasm 2.92 4.36 3.50 1.88 -3.28 -4.63 3.24 3.14 6.92 -0.72 -2.53 4.97 2.83 5.98 -3.49
Cytoplasmic Foci 2.26 2.74 2.55 2.14 -2.61 -4.38 2.96 3.43 4.90 1.71 -3.38 3.76 1.80 4.90 -2.50
Eisosomes 2.85 0.73 1.72 -5.72 2.76 -5.52 -6.90 -7.86 1.02 3.16 -5.26 -9.70 -9.19 -3.56 3.47
Endoplasmic Reticulum -4.23 -6.89 -7.95 -5.36 1.48 -4.08 -7.84 -8.28 -4.38 2.09 -8.27 -11.35 -15.88 -7.46 2.02
Endosome 0.70 1.62 -0.98 -2.24 -3.55 -4.71 -2.43 -3.05 4.51 0.40 -5.05 -1.18 -3.76 -0.46 -3.58
Golgi 0.22 1.36 -0.35 -0.69 -2.06 -2.20 0.91 0.80 2.57 -2.22 -2.23 1.01 -1.27 0.79 -2.11
Lipid Particles 2.82 3.25 2.60 -0.21 -1.40 -3.58 1.01 1.83 4.51 2.49 -2.64 1.83 0.19 2.59 -1.29
Mitochondria -2.97 -1.66 -5.42 -1.21 -5.06 -3.77 -1.53 -4.62 3.18 -3.92 -4.74 -2.77 -6.57 1.84 -5.77
Mitotic Spindle -1.91 -0.52 -6.85 -5.84 -6.81 -3.27 1.10 -1.86 1.91 -2.55 -3.26 0.92 -6.68 -4.47 -7.12
None 1.27 2.56 1.64 1.10 -6.54 -1.33 4.39 -0.10 0.96 -2.83 -1.16 5.58 0.22 1.47 -6.76
Nuclear Periphery -4.36 -2.70 -7.59 -5.56 -6.84 -3.26 1.60 -0.15 3.88 -3.83 -1.80 1.69 -3.67 -2.58 -7.27
Nuclear Periphery Foci 1.43 1.71 1.30 -0.80 -3.12 -0.41 1.16 0.85 2.84 -1.33 0.04 1.39 0.03 -0.02 -3.64
Nucleolus 1.55 0.74 2.78 2.83 1.81 -2.91 4.26 4.20 5.89 -0.46 -2.24 0.25 2.80 5.48 1.41
Nucleus -2.18 -2.82 0.60 7.48 9.15 8.63 -0.33 3.56 -5.95 3.58 7.43 0.13 10.73 3.08 10.64
Peroxisomes 0.33 1.58 1.64 2.14 0.57 -4.77 0.37 2.31 5.08 2.21 -3.58 0.05 0.20 3.66 0.22
Vacuole 0.52 -2.91 -7.51 -8.08 -5.45 -4.96 -6.16 -4.02 -0.83 -0.93 -6.11 -7.90 -9.77 -7.36 -5.70
Vacuole Periphery -1.72 0.96 -2.23 -0.18 -2.72 -2.27 1.10 0.85 2.45 -1.46 -2.95 0.77 -2.43 0.81 -2.80
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Prp22

Prp22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available