Standard name | |
---|---|
Human Ortholog | |
Description | Microtubule plus end-tracking protein; together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally; homolog of human end binding protein 1 (EB1) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 4 | 3 |
Bud | 3 | 0 | 1 | 2 | 0 | 1 | 7 | 11 | 6 | 4 | 9 | 7 | 2 | 0 | 0 | 2 | 2 | 1 | 4 | 5 | 1 | 1 | 4 | 11 |
Bud Neck | 3 | 1 | 1 | 2 | 6 | 4 | 5 | 10 | 5 | 6 | 9 | 6 | 2 | 0 | 0 | 1 | 3 | 0 | 2 | 2 | 3 | 2 | 5 | 3 |
Bud Site | 13 | 0 | 0 | 4 | 2 | 10 | 14 | 12 | 14 | 16 | 18 | 16 | 2 | 2 | 3 | 0 | 2 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 4 | 1 | 8 | 17 | 8 | 14 | 16 | 7 | 8 | 7 | 8 | 3 | 8 | 17 | 6 | 3 | 7 | 0 | 2 | 0 | 0 | 3 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 69 | 4 | 4 | 29 | 52 | 38 | 42 | 22 | 9 | 11 | 7 | 11 | 51 | 90 | 103 | 44 | 25 | 46 | 51 | 15 | 18 | 25 | 38 | 50 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 |
Mitochondria | 63 | 40 | 6 | 20 | 18 | 51 | 95 | 84 | 88 | 74 | 126 | 89 | 6 | 0 | 2 | 15 | 8 | 13 | 11 | 8 | 2 | 9 | 11 | 6 |
Nucleus | 12 | 3 | 4 | 24 | 15 | 24 | 32 | 37 | 27 | 26 | 39 | 26 | 8 | 7 | 9 | 7 | 2 | 8 | 4 | 6 | 1 | 1 | 3 | 5 |
Nuclear Periphery | 0 | 2 | 0 | 1 | 0 | 0 | 6 | 2 | 0 | 3 | 4 | 1 | 0 | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 123 | 50 | 22 | 65 | 140 | 87 | 150 | 147 | 105 | 106 | 123 | 126 | 96 | 124 | 112 | 94 | 80 | 112 | 10 | 11 | 3 | 6 | 8 | 12 |
Peroxisomes | 23 | 0 | 2 | 8 | 4 | 7 | 14 | 7 | 6 | 5 | 1 | 1 | 3 | 17 | 35 | 11 | 9 | 7 | 19 | 2 | 0 | 5 | 25 | 26 |
SpindlePole | 155 | 13 | 32 | 113 | 198 | 97 | 139 | 137 | 64 | 78 | 75 | 70 | 113 | 170 | 153 | 62 | 59 | 62 | 169 | 48 | 61 | 67 | 140 | 182 |
Vac/Vac Membrane | 7 | 15 | 1 | 11 | 13 | 17 | 5 | 2 | 8 | 5 | 1 | 6 | 4 | 5 | 14 | 17 | 11 | 12 | 3 | 4 | 3 | 1 | 0 | 2 |
Unique Cell Count | 284 | 98 | 53 | 193 | 307 | 222 | 337 | 339 | 229 | 224 | 272 | 241 | 185 | 260 | 265 | 158 | 126 | 176 | 310 | 113 | 102 | 135 | 265 | 332 |
Labelled Cell Count | 481 | 133 | 75 | 289 | 465 | 344 | 523 | 489 | 339 | 344 | 419 | 368 | 290 | 423 | 451 | 261 | 207 | 276 | 310 | 113 | 102 | 135 | 265 | 332 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 6.2 | 6.5 | 6.2 | 6.8 | 5.5 | 5.3 | 5.9 | 5.1 | 5.4 | 5.0 | 5.1 | 7.0 | 7.6 | 7.5 | 9.2 | 10.4 | 10.5 | 6.2 | 6.9 | 7.4 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.1 | 1.2 | 1.3 | 1.0 | 1.3 | 1.3 | 1.1 | 1.2 | 1.2 | 1.0 | 0.9 | 1.3 | 1.6 | 1.9 | 2.1 | 2.4 | 1.4 | 1.1 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.06 | -0.23 | -0.29 | -0.13 | -0.37 | -0.28 | -0.39 | -0.35 | 0.1 | 0.22 | 0.2 | 0.5 | 0.67 | 0.69 | -0.08 | 0.08 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.4 | 1.7 | 1.7 | 1.0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.9 | 4.4 | 3.1 | 2.0 | 2.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.2 | 0 | 1.9 | 2.6 | 2.2 | 3.8 | 3.1 | 4.7 | 3.6 | 0 | 0 | 0 | -0.4 | 0 | 0 |
Nucleus | 1.0 | 0 | 0.7 | 0 | 0.7 | 0.9 | 0.9 | 1.3 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.1 | 0.6 | -0.3 | 0.4 | 0.3 | 0.6 | 0.8 | 0.5 | 1.4 | 1.3 | 0.8 | 0.1 | 2.3 | 2.7 | 2.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 |
SpindlePole | -0.2 | 0.6 | -2.2 | -2.6 | -2.7 | -4.5 | -3.4 | -4.6 | -4.3 | 0.1 | 0.7 | -0.4 | -2.7 | -1.7 | -3.3 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7303 | 4.7556 | 4.815 | 4.3658 | 3.6397 | 4.1399 | 3.3026 | 4.7597 | 4.5442 | 3.8224 | 3.348 | 4.065 | 1.656 | 2.8058 | 2.7188 | 2.6369 | 1.7366 | 2.2479 |
Actin | 0.0106 | 0.0005 | 0.0084 | 0.0015 | 0.007 | 0.0095 | 0.0244 | 0.0007 | 0.0026 | 0.0013 | 0.0288 | 0.0111 | 0.0148 | 0.0005 | 0.0005 | 0.0013 | 0.0486 | 0.0271 |
Bud | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0006 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0006 | 0.0007 | 0.0004 | 0.0006 | 0.0009 | 0.0003 | 0.0002 | 0.0015 | 0.0008 |
Bud Neck | 0.0009 | 0.0026 | 0.001 | 0.0002 | 0.0011 | 0.0127 | 0.002 | 0.0018 | 0.0009 | 0.0034 | 0.0018 | 0.0059 | 0.0043 | 0.0025 | 0.0046 | 0.0024 | 0.0104 | 0.0233 |
Bud Periphery | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0009 | 0.0008 | 0.0007 | 0.0005 | 0.0003 | 0.0014 | 0.0021 | 0.0013 | 0.001 | 0.0009 | 0.0006 | 0.0003 | 0.0031 | 0.0036 |
Bud Site | 0.0015 | 0.0025 | 0.0017 | 0.0002 | 0.0028 | 0.0023 | 0.0031 | 0.0126 | 0.0009 | 0.005 | 0.0027 | 0.0017 | 0.0098 | 0.0081 | 0.0026 | 0.0013 | 0.0087 | 0.0017 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0011 |
Cytoplasm | 0.0045 | 0.0123 | 0.0009 | 0.0001 | 0.0014 | 0.0014 | 0.0029 | 0.0002 | 0.0002 | 0.0015 | 0.0014 | 0.0015 | 0.002 | 0.0022 | 0.0004 | 0.0001 | 0.0013 | 0.0036 |
Cytoplasmic Foci | 0.051 | 0.0205 | 0.0299 | 0.0537 | 0.0802 | 0.0247 | 0.0493 | 0.0157 | 0.0227 | 0.067 | 0.0347 | 0.0294 | 0.083 | 0.0221 | 0.0309 | 0.0829 | 0.0549 | 0.0371 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0 | 0 | 0.0001 | 0.0009 | 0.0003 |
Endoplasmic Reticulum | 0.0008 | 0.0002 | 0.0006 | 0.0001 | 0.0015 | 0.0011 | 0.0011 | 0 | 0.0001 | 0.0055 | 0.0011 | 0.0008 | 0.0006 | 0.0004 | 0 | 0.0001 | 0.0004 | 0.0014 |
Endosome | 0.0152 | 0.0012 | 0.0108 | 0.0032 | 0.0722 | 0.0105 | 0.0131 | 0.002 | 0.0022 | 0.0465 | 0.0203 | 0.0174 | 0.019 | 0.0049 | 0.0008 | 0.0042 | 0.0385 | 0.0195 |
Golgi | 0.008 | 0.0019 | 0.0036 | 0.0045 | 0.0282 | 0.009 | 0.008 | 0.0021 | 0.0032 | 0.0086 | 0.0188 | 0.0075 | 0.0126 | 0.0034 | 0.0014 | 0.0084 | 0.0536 | 0.0133 |
Lipid Particles | 0.0839 | 0.0236 | 0.0252 | 0.0753 | 0.2769 | 0.0953 | 0.0706 | 0.0266 | 0.0295 | 0.1498 | 0.2519 | 0.0649 | 0.0818 | 0.059 | 0.0164 | 0.1604 | 0.2915 | 0.0975 |
Mitochondria | 0.0029 | 0.0001 | 0.0048 | 0.0469 | 0.0082 | 0.0217 | 0.0034 | 0.0002 | 0.0121 | 0.0062 | 0.1823 | 0.023 | 0.0322 | 0.0006 | 0.001 | 0.0135 | 0.1477 | 0.0216 |
None | 0.0023 | 0.0028 | 0.0008 | 0.0001 | 0.0008 | 0.0007 | 0.0033 | 0.0001 | 0.0003 | 0.0016 | 0.0063 | 0.0005 | 0.0014 | 0.0005 | 0.0004 | 0.0003 | 0.0013 | 0.0008 |
Nuclear Periphery | 0.017 | 0.0036 | 0.0087 | 0.0016 | 0.0222 | 0.0261 | 0.0122 | 0.0028 | 0.0014 | 0.0183 | 0.0106 | 0.0143 | 0.0144 | 0.0077 | 0.0021 | 0.0023 | 0.0032 | 0.0292 |
Nucleolus | 0.0447 | 0.1205 | 0.0735 | 0.0071 | 0.0096 | 0.0944 | 0.084 | 0.1551 | 0.0787 | 0.1207 | 0.1221 | 0.0846 | 0.0471 | 0.1577 | 0.1149 | 0.0674 | 0.0727 | 0.0391 |
Nucleus | 0.0158 | 0.0464 | 0.0134 | 0.0014 | 0.0028 | 0.0795 | 0.0362 | 0.0593 | 0.0161 | 0.0089 | 0.0188 | 0.0604 | 0.0223 | 0.0919 | 0.0161 | 0.0067 | 0.0253 | 0.1469 |
Peroxisomes | 0.0132 | 0.005 | 0.0059 | 0.0085 | 0.0863 | 0.0226 | 0.0215 | 0.0078 | 0.0167 | 0.0439 | 0.1397 | 0.016 | 0.0271 | 0.0068 | 0.0112 | 0.0744 | 0.1539 | 0.0399 |
Punctate Nuclear | 0.7256 | 0.7555 | 0.8094 | 0.7946 | 0.3906 | 0.5845 | 0.6617 | 0.7116 | 0.8114 | 0.4895 | 0.1482 | 0.6546 | 0.6228 | 0.6279 | 0.7953 | 0.573 | 0.0771 | 0.4859 |
Vacuole | 0.0007 | 0.0003 | 0.0006 | 0.0002 | 0.0043 | 0.0013 | 0.001 | 0.0003 | 0.0002 | 0.017 | 0.0031 | 0.003 | 0.0014 | 0.0016 | 0.0002 | 0.0004 | 0.0031 | 0.004 |
Vacuole Periphery | 0.0005 | 0.0001 | 0.0004 | 0.0004 | 0.0021 | 0.0013 | 0.0008 | 0.0001 | 0.0002 | 0.0026 | 0.0037 | 0.0012 | 0.0009 | 0.0004 | 0.0001 | 0.0003 | 0.0018 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.0531 | 14.2953 | 14.8508 | 20.5446 | 44.9226 | 12.786 | 18.5493 | 15.9339 | 19.0111 | 31.1166 |
Translational Efficiency | 1.6464 | 1.6668 | 0.7986 | 0.8603 | 0.8612 | 2.8985 | 1.2334 | 1.0292 | 1.1052 | 1.1332 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2256 | 564 | 2193 | 416 | 1458 | 1184 | 58 | 968 | 3714 | 1748 | 2251 | 1384 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 837.90 | 964.20 | 984.89 | 1071.50 | 802.54 | 899.95 | 1174.75 | 1078.83 | 824.02 | 920.68 | 989.78 | 1076.63 |
Standard Deviation | 102.88 | 158.42 | 139.48 | 178.75 | 108.50 | 131.05 | 127.97 | 177.11 | 106.53 | 143.64 | 142.41 | 177.63 |
Intensity Change Log 2 | — | 0.202554 | 0.233185 | 0.354782 | — | 0.165272 | 0.549709 | 0.426822 | — | 0.184435 | 0.396708 | 0.390475 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000757 | 0.002487 | 0.000337 | 0.000899 | 0.001049 | 0.001199 | 0.003442 | 0.001743 | 0.000872 | 0.001615 | 0.000417 | 0.001489 |
Bud Neck | 0.006904 | 0.007938 | 0.004445 | 0.010184 | 0.006256 | 0.006859 | 0.012366 | 0.009419 | 0.006650 | 0.007207 | 0.004649 | 0.009649 |
Bud Site | 0.006825 | 0.011702 | 0.004060 | 0.023038 | 0.008033 | 0.007938 | 0.020158 | 0.016440 | 0.007300 | 0.009152 | 0.004475 | 0.018424 |
Cell Periphery | 0.000168 | 0.000156 | 0.000036 | 0.000166 | 0.000179 | 0.000137 | 0.000182 | 0.000120 | 0.000172 | 0.000143 | 0.000040 | 0.000134 |
Cytoplasm | 0.007029 | 0.012925 | 0.002613 | 0.011015 | 0.006977 | 0.010053 | 0.014066 | 0.006509 | 0.007008 | 0.010980 | 0.002908 | 0.007864 |
Cytoplasmic Foci | 0.050571 | 0.032183 | 0.002514 | 0.023340 | 0.040074 | 0.028918 | 0.009710 | 0.010920 | 0.046450 | 0.029972 | 0.002700 | 0.014653 |
Eisosomes | 0.000081 | 0.000070 | 0.000011 | 0.000024 | 0.000090 | 0.000060 | 0.000073 | 0.000023 | 0.000085 | 0.000063 | 0.000013 | 0.000023 |
Endoplasmic Reticulum | 0.001575 | 0.002467 | 0.000401 | 0.000572 | 0.002027 | 0.001936 | 0.003733 | 0.000788 | 0.001752 | 0.002107 | 0.000487 | 0.000723 |
Endosome | 0.012375 | 0.007713 | 0.001019 | 0.009667 | 0.008795 | 0.010610 | 0.005722 | 0.006670 | 0.010970 | 0.009676 | 0.001140 | 0.007571 |
Golgi | 0.009038 | 0.006724 | 0.000922 | 0.008024 | 0.010083 | 0.007610 | 0.001759 | 0.005119 | 0.009448 | 0.007324 | 0.000944 | 0.005992 |
Lipid Particles | 0.051656 | 0.017664 | 0.000973 | 0.000597 | 0.042733 | 0.020672 | 0.003719 | 0.001107 | 0.048153 | 0.019701 | 0.001044 | 0.000953 |
Mitochondria | 0.005775 | 0.003141 | 0.001369 | 0.006430 | 0.003932 | 0.006523 | 0.002641 | 0.012464 | 0.005051 | 0.005432 | 0.001401 | 0.010650 |
Mitotic Spindle | 0.657846* | 0.754047* | 0.948965* | 0.852053* | 0.688295* | 0.769894* | 0.782583* | 0.882187* | 0.669799* | 0.764781* | 0.944678* | 0.873130* |
None | 0.002558 | 0.001545 | 0.000522 | 0.000892 | 0.002140 | 0.001505 | 0.000771 | 0.000783 | 0.002394 | 0.001518 | 0.000528 | 0.000816 |
Nuclear Periphery | 0.000885 | 0.000841 | 0.000555 | 0.000559 | 0.000745 | 0.000764 | 0.000657 | 0.000812 | 0.000830 | 0.000789 | 0.000558 | 0.000736 |
Nuclear Periphery Foci | 0.056641 | 0.052157 | 0.005229 | 0.001507 | 0.063646 | 0.038780 | 0.020188 | 0.003377 | 0.059391 | 0.043096 | 0.005614 | 0.002815 |
Nucleolus | 0.047736 | 0.029661 | 0.011926 | 0.016624 | 0.043202 | 0.035261 | 0.016247 | 0.009295 | 0.045956 | 0.033454 | 0.012037 | 0.011498 |
Nucleus | 0.012750 | 0.021564 | 0.009480 | 0.012261 | 0.015670 | 0.018323 | 0.029065 | 0.011374 | 0.013896 | 0.019369 | 0.009984 | 0.011641 |
Peroxisomes | 0.066880 | 0.031922 | 0.003550 | 0.009439 | 0.054493 | 0.029059 | 0.063180 | 0.008117 | 0.062017 | 0.029983 | 0.005086 | 0.008515 |
Vacuole | 0.001551 | 0.002604 | 0.000851 | 0.008866 | 0.001326 | 0.002551 | 0.009173 | 0.008096 | 0.001463 | 0.002568 | 0.001065 | 0.008327 |
Vacuole Periphery | 0.000399 | 0.000490 | 0.000222 | 0.003844 | 0.000256 | 0.001347 | 0.000565 | 0.004637 | 0.000343 | 0.001071 | 0.000231 | 0.004399 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.84 | 3.85 | -1.92 | 2.23 | -3.28 | -0.85 | -2.53 | -2.27 | -1.95 | 1.69 | -3.36 | 4.14 | -2.95 | -0.20 | -3.88 |
Bud Neck | -1.76 | -0.13 | -5.10 | -3.63 | -4.97 | -1.96 | -2.34 | -6.65 | -5.29 | -0.10 | -2.39 | -0.89 | -8.26 | -6.38 | -7.19 |
Bud Site | -4.03 | 1.57 | -5.91 | -3.78 | -6.22 | -1.10 | -2.84 | -6.55 | -5.77 | 0.34 | -3.69 | 1.55 | -9.13 | -6.76 | -9.54 |
Cell Periphery | -0.61 | 12.03 | -0.35 | -0.12 | -3.16 | 2.28 | -1.11 | 2.47 | 0.35 | 1.81 | 1.68 | 13.96 | 1.38 | 0.21 | -5.55 |
Cytoplasm | -2.04 | 3.43 | -2.22 | -0.54 | -3.27 | -1.81 | -1.62 | -1.13 | 0.68 | 1.36 | -2.61 | 3.32 | -2.27 | 0.21 | -4.14 |
Cytoplasmic Foci | 5.02 | 21.69 | 5.80 | 1.25 | -6.28 | 3.70 | 10.15 | 8.84 | 4.83 | -2.41 | 7.21 | 26.25 | 12.56 | 4.68 | -8.59 |
Eisosomes | 1.84 | 27.29 | 15.54 | 5.73 | -4.87 | 6.75 | 0.38 | 16.34 | 9.56 | 3.18 | 6.48 | 30.88 | 24.07 | 10.88 | -5.40 |
Endoplasmic Reticulum | -4.37 | 12.47 | 4.87 | 6.76 | -2.07 | 1.38 | -2.34 | 3.98 | 2.57 | 2.80 | -2.38 | 12.18 | 4.39 | 5.37 | -3.42 |
Endosome | 3.50 | 13.58 | 2.68 | 0.36 | -3.54 | -0.90 | 1.56 | 2.41 | 2.62 | 0.13 | 1.63 | 17.25 | 4.36 | 2.47 | -5.34 |
Golgi | 2.81 | 16.92 | 2.10 | 0.82 | -2.25 | 2.95 | 9.75 | 5.10 | 2.49 | -1.34 | 3.89 | 18.21 | 5.52 | 2.53 | -3.32 |
Lipid Particles | 9.15 | 18.94 | 19.09 | 6.13 | 1.03 | 5.70 | 11.69 | 13.21 | 8.22 | 2.71 | 10.64 | 23.04 | 23.06 | 10.15 | -0.45 |
Mitochondria | 1.18 | 5.51 | -0.36 | -1.24 | -3.06 | -1.88 | 1.50 | -3.02 | -1.86 | -3.51 | -0.36 | 5.54 | -2.73 | -2.21 | -4.46 |
Mitotic Spindle | -5.75 | -35.75* | -10.30 | -3.86 | 8.36 | -6.99 | -0.94 | -15.41 | -7.99 | -3.21 | -10.18 | -40.18 | -20.54 | -8.80 | 10.48 |
None | 3.04 | 6.32 | 4.41 | 1.52 | -1.17 | 1.02 | 1.47 | 4.86 | 3.41 | 0.67 | 2.62 | 7.25 | 6.98 | 3.75 | -0.77 |
Nuclear Periphery | 0.65 | 3.27 | -0.65 | -0.94 | -1.55 | -0.70 | -1.48 | -4.56 | -4.08 | -1.24 | 0.85 | 2.28 | -3.49 | -3.48 | -4.37 |
Nuclear Periphery Foci | 1.21 | 20.96* | 23.61* | 11.05 | 5.98 | 6.03 | 1.55 | 18.16 | 11.90 | 2.70 | 5.79 | 25.74 | 28.77 | 16.09 | 4.03 |
Nucleolus | 4.42 | 12.90 | 7.43 | 2.47 | -1.92 | 2.53 | 3.38 | 10.99 | 8.00 | 1.71 | 4.67 | 15.62 | 14.69 | 8.11 | -0.12 |
Nucleus | -3.17 | -0.78 | -3.70 | -1.26 | -3.31 | -1.63 | -2.47 | -2.56 | -1.18 | 2.04 | -3.66 | -0.87 | -4.63 | -1.66 | -3.61 |
Peroxisomes | 6.36 | 20.48 | 15.48 | 5.09 | -4.55 | 5.89 | 0.74 | 13.57 | 7.64 | 1.90 | 9.46 | 24.57 | 21.41 | 9.21 | -4.12 |
Vacuole | -4.20 | 0.31 | -4.10 | -3.65 | -4.13 | -3.50 | -2.57 | -6.29 | -5.43 | 0.95 | -4.70 | -1.61 | -7.39 | -6.48 | -7.08 |
Vacuole Periphery | -1.22 | 1.04 | -2.32 | -2.17 | -2.40 | -2.04 | -2.83 | -3.24 | -2.18 | -2.78 | -2.08 | 0.57 | -3.86 | -2.87 | -3.91 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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