Standard name
Human Ortholog
Description Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging; expression of human homolog AFG3L2 can complement yeast yta12 afg3 double mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.05 0.12 0.09 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0.14 0.05 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05
Golgi 0.07 0 0 0 0 0 0 0 – 0 0 0 0.14 0.37 0.52 0.09 0.1 0.11 0 0.09 0 0.06 0.06 0.09
Mitochondria 0.87 0.94 0.93 0.92 0.82 0.9 0.89 0.88 – 0.93 0.93 0.94 0.86 0.7 0.61 0.11 0.1 0.05 0.84 0.72 0.76 0.75 0.69 0.65
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0.07 0.08 0.06 0.09 0.09 0.15 0.13 – 0.14 0.09 0.11 0.06 0 0 0.1 0.21 0.15 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.07 0.09 0.11 0.13 – 0.1 0.07 0.07 0 0 0 0 0.05 0.12 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0 0 0 0 0 – 0 0 0 0 0 0 0.21 0.12 0.16 0 0 0.12 0.07 0.09 0.09
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.09 0 0 0.07 0 0 0 – 0 0 0 0.12 0.2 0.18 0.16 0.14 0.15 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 1 3 1 0 0 0 – 0 1 1 1 9 5 1 1 2 4 3 3 3 15 12
Bud 1 2 8 5 1 6 5 19 – 11 13 9 1 1 0 8 2 3 0 1 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud Site 0 0 1 0 0 4 5 6 – 7 4 1 0 0 0 1 3 1 – – – – – –
Cell Periphery 11 4 8 3 3 0 0 4 – 2 2 2 1 7 9 16 5 8 0 1 0 1 0 1
Cytoplasm 4 0 9 13 13 16 9 9 – 4 6 4 4 3 6 19 3 10 0 0 0 0 0 0
Endoplasmic Reticulum 2 3 3 4 4 7 5 7 – 6 1 7 1 5 7 5 2 2 0 0 1 0 0 1
Endosome 9 1 5 2 17 3 3 7 – 2 0 1 3 3 3 6 1 4 8 7 8 9 9 15
Golgi 27 9 4 2 2 0 0 0 – 0 0 0 15 54 100 12 6 8 18 22 13 13 17 27
Mitochondria 347 217 341 267 222 375 399 539 – 472 291 389 93 102 118 15 6 4 355 170 284 165 204 210
Nucleus 0 2 0 2 0 2 2 4 – 1 0 0 0 0 1 8 5 2 0 0 0 0 0 0
Nuclear Periphery 30 16 28 18 25 36 67 77 – 70 27 47 7 5 6 13 12 11 6 3 3 1 5 5
Nucleolus 16 8 13 7 20 38 48 78 – 52 22 31 1 0 0 5 3 9 1 1 1 1 1 2
Peroxisomes 13 3 28 5 7 2 9 9 – 6 4 6 4 3 5 28 7 12 18 6 46 16 26 29
SpindlePole 1 0 4 0 3 3 9 8 – 3 4 3 0 1 1 6 6 1 2 0 3 1 0 0
Vac/Vac Membrane 55 20 11 5 20 16 13 13 – 17 8 10 13 29 34 21 8 11 4 15 0 2 9 7
Unique Cell Count 398 232 368 290 272 415 449 610 509 312 415 108 145 193 134 58 74 423 238 373 222 298 322
Labelled Cell Count 516 288 464 336 338 508 574 780 653 383 511 144 222 295 164 70 88 423 238 373 222 298 322


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.6 8.9 7.9 8.5 8.7 7.6 7.4 7.7 – 7.5 7.3 7.7 9.6 9.9 10.2 7.2 7.6 9.8 9.7 10.4 10.9
Std Deviation (1e-4) 1.8 1.9 2.0 2.0 2.3 2.4 2.2 2.4 – 2.5 2.5 2.5 2.0 1.8 1.8 1.6 2.0 2.6 2.2 2.5 2.3
Intensity Change (Log2) – – – 0.1 0.14 -0.06 -0.09 -0.03 – -0.07 -0.11 -0.04 0.28 0.34 0.38 -0.14 -0.04 0.32 0.3 0.4 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud -0.4 0 -0.8 -1.2 0.9 – 0 1.5 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 1.6 4.5 0 0
Cytoplasm 1.4 1.6 1.1 -0.4 -1.1 – -2.0 -0.5 -1.6 0 0 0.5 5.1 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 3.4 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 11.6 14.7 0 0 0
Mitochondria -0.3 -4.2 -1.1 -1.8 -2.2 – 0 0.3 0.6 -2.1 -6.6 -9.2 -17.8 -15.0 -16.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.7 0.7 0.5 3.2 2.5 – 2.8 0.5 1.8 -0.4 -1.7 -2.1 0.8 3.2 2.0
Nucleolus -0.8 2.2 3.2 3.9 4.8 – 3.7 2.1 2.4 0 0 0 0 0 0
Peroxisomes -3.4 -2.8 -5.2 -3.8 -4.9 – -4.9 -3.9 -4.2 -1.4 -2.4 -2.4 4.2 0 2.4
SpindlePole 0 0 0 1.0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.0 2.5 0.7 -0.1 -0.8 – 0.3 -0.3 -0.5 3.8 6.5 6.1 5.1 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0371 0.0211 0.0363 0.0548 0.0014 0.0009 0.0115 0.0043 0.004 0.0001 0.0241 0.0017 0.0042 0.0071 0.0239 0.0007 0.0001 0.0015
Bud 0.0008 0.0024 0.0005 0.0019 0.0008 0 0.0003 0.0013 0.0001 0 0.0003 0 0.0008 0.0002 0.0003 0.0001 0 0
Bud Neck 0.0007 0.0005 0.0003 0.0003 0.0001 0 0.0003 0.0003 0 0 0.0002 0 0.0003 0.0001 0.0001 0 0 0
Bud Periphery 0.0019 0.0029 0.0009 0.004 0.0044 0.0001 0.0007 0.0013 0.0003 0.0001 0.0008 0.0001 0.0011 0.0003 0.0006 0.0002 0 0.0001
Bud Site 0.0046 0.0159 0.001 0.0032 0.0001 0 0.0011 0.0089 0.0001 0 0.0006 0 0.0021 0.0014 0.002 0.0001 0 0
Cell Periphery 0.001 0.0011 0.0001 0.0001 0.0004 0.0001 0.0003 0.0001 0.0003 0 0.0001 0 0.0002 0.0002 0.0001 0 0 0.0001
Cytoplasm 0.001 0.0004 0.0006 0.0001 0 0 0.002 0.0001 0.0006 0 0.0003 0 0.0039 0.0001 0 0 0 0
Cytoplasmic Foci 0.0209 0.0043 0.0035 0.0066 0.0021 0.0025 0.0062 0.0047 0.0008 0.0001 0.0037 0.0008 0.0258 0.0186 0.002 0.0019 0.0002 0.0014
Eisosomes 0.0012 0.0002 0.0003 0.0002 0.0001 0.0001 0.0004 0.0001 0.0007 0 0.0004 0.0001 0.0001 0.0005 0.0004 0 0 0.0003
Endoplasmic Reticulum 0.003 0.0003 0.0003 0.0001 0.0001 0.0001 0.0033 0.0001 0.0011 0 0.0016 0 0.0068 0 0 0 0 0.0001
Endosome 0.0268 0.0098 0.0047 0.0063 0.0083 0.0039 0.0135 0.0132 0.0022 0.0004 0.0148 0.0026 0.1982 0.0195 0.0006 0.0019 0.0001 0.0078
Golgi 0.0309 0.0119 0.0121 0.0162 0.0436 0.0102 0.0355 0.035 0.0047 0.0008 0.0151 0.0172 0.0316 0.0183 0.0027 0.0038 0.0003 0.0514
Lipid Particles 0.0205 0.0038 0.0053 0.0294 0.0071 0.0304 0.0166 0.0064 0.0012 0.0002 0.0361 0.0079 0.0743 0.0087 0.0019 0.0005 0.0007 0.0385
Mitochondria 0.8156 0.8721 0.9224 0.8664 0.919 0.931 0.8719 0.9102 0.9688 0.9973 0.8857 0.9614 0.507 0.874 0.9356 0.9753 0.9881 0.8881
None 0.0019 0.0001 0.0002 0.0001 0 0.0001 0.0031 0 0.0009 0 0.0005 0 0.003 0.0001 0.0001 0 0 0
Nuclear Periphery 0.0051 0.0006 0.0005 0.0001 0.0001 0.0006 0.0047 0 0.0023 0 0.0048 0 0.078 0 0 0 0 0.0001
Nucleolus 0.0005 0.0004 0.0001 0.0001 0.0001 0.0007 0.0004 0.0001 0.0001 0 0.0005 0 0.0026 0.0002 0.0001 0 0 0.0002
Nucleus 0.0009 0.0003 0.0001 0.0001 0.0001 0.0001 0.0016 0.0001 0.0006 0 0.0007 0 0.0068 0.0001 0 0 0 0.0001
Peroxisomes 0.0107 0.0172 0.0057 0.0083 0.0017 0.0031 0.0207 0.0096 0.0082 0.0006 0.0042 0.0054 0.0045 0.0484 0.0291 0.015 0.0101 0.005
Punctate Nuclear 0.0048 0.0001 0.0024 0.0001 0 0.0063 0.0014 0 0.0016 0 0.0023 0 0.0152 0.0001 0 0 0 0
Vacuole 0.004 0.0151 0.0008 0.0008 0.0027 0.0018 0.0018 0.0011 0.0004 0.0001 0.001 0.0003 0.016 0.001 0.0001 0.0001 0 0.0004
Vacuole Periphery 0.0062 0.0193 0.0019 0.0009 0.0077 0.008 0.0027 0.0029 0.0012 0.0003 0.0023 0.0023 0.0175 0.0009 0.0002 0.0003 0.0001 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.9596 40.5597 26.2548 35.2336 38.1747 30.3919 44.1728 49.4686 40.8083 43.3682
Translational Efficiency 0.6315 0.5262 0.7793 0.6025 0.4586 0.6914 0.5127 0.4692 0.581 0.4798

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
190 982 293 1110 2274 1970 526 2018 2464 2952 819 3128

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1376.64 932.07 1299.35 1314.24 946.74 1013.64 1094.54 1334.30 979.89 986.51 1167.81 1327.18
Standard Deviation 251.86 180.78 182.87 239.25 240.33 162.48 248.87 198.72 267.11 173.11 247.75 214.20
Intensity Change Log 2 — -0.562641 -0.083361 -0.066923 — 0.098505 0.209284 0.495043 — -0.255928 0.043132 0.188972

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.010243 0.008297 0.004808 0.012240 0.001028 0.001507 0.003428 0.002380 0.001739 0.003766 0.003922 0.005879
Bud Neck 0.004396 0.002588 0.006053 0.001610 0.000482 0.000638 0.004452 0.000379 0.000783 0.001287 0.005025 0.000816
Bud Site 0.009741 0.005863 0.011755 0.004982 0.001145 0.001495 0.008873 0.002000 0.001807 0.002948 0.009904 0.003058
Cell Periphery 0.002227 0.000516 0.000328 0.001223 0.000174 0.000115 0.000425 0.000263 0.000333 0.000248 0.000390 0.000603
Cytoplasm 0.000531 0.001359 0.001028 0.000570 0.001214 0.000360 0.000762 0.000293 0.001161 0.000693 0.000857 0.000392
Cytoplasmic Foci 0.019032 0.018606 0.080620 0.006302 0.005603 0.011171 0.020042 0.007301 0.006639 0.013644 0.041714 0.006946
Eisosomes 0.000333 0.000217 0.000091 0.000376 0.000050 0.000063 0.000164 0.000072 0.000072 0.000114 0.000138 0.000180
Endoplasmic Reticulum 0.004190 0.000373 0.000999 0.000633 0.000145 0.000124 0.000356 0.000229 0.000457 0.000207 0.000586 0.000373
Endosome 0.049580 0.029586 0.057738 0.005916 0.009032 0.014877 0.024278 0.007133 0.012158 0.019770 0.036248 0.006701
Golgi 0.262773* 0.035121 0.105639 0.029677 0.012053 0.022932 0.035982 0.049531 0.031386 0.026987 0.060902 0.042486
Lipid Particles 0.003647 0.006211 0.006763 0.001027 0.002751 0.003345 0.005772 0.000662 0.002820 0.004299 0.006126 0.000791
Mitochondria 0.497321* 0.846022* 0.538057* 0.906213* 0.956485* 0.923965* 0.824147* 0.904231* 0.921078* 0.898037* 0.721797* 0.904934*
Mitotic Spindle 0.005563 0.001832 0.055440 0.001570 0.000388 0.001281 0.016045 0.001727 0.000787 0.001464 0.030139 0.001671
None 0.000751 0.000459 0.000671 0.000954 0.000784 0.000227 0.000629 0.000637 0.000782 0.000304 0.000644 0.000749
Nuclear Periphery 0.001325 0.000096 0.002943 0.000149 0.000079 0.000065 0.000730 0.000222 0.000176 0.000075 0.001522 0.000196
Nuclear Periphery Foci 0.001126 0.000432 0.002693 0.000094 0.000099 0.000231 0.000573 0.000132 0.000178 0.000298 0.001332 0.000118
Nucleolus 0.000595 0.001351 0.000768 0.000098 0.000058 0.000791 0.000467 0.000138 0.000099 0.000977 0.000574 0.000124
Nucleus 0.000454 0.000837 0.000577 0.000036 0.000233 0.000246 0.000406 0.000033 0.000250 0.000443 0.000467 0.000034
Peroxisomes 0.004719 0.029176 0.023755 0.011648 0.004399 0.009840 0.023201 0.001892 0.004424 0.016272 0.023399 0.005354
Vacuole 0.057308 0.006788 0.047351 0.005135 0.001286 0.003580 0.014526 0.007287 0.005606 0.004647 0.026269 0.006523
Vacuole Periphery 0.064144 0.004269 0.051923 0.009550 0.002513 0.003147 0.014743 0.013458 0.007265 0.003520 0.028044 0.012071

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.46 1.30 -0.45 -1.77 -3.59 -1.91 -3.14 -2.82 -1.84 1.22 -3.59 -3.25 -5.18 -2.52 -2.10
Bud Neck 0.78 -0.71 1.22 1.69 4.91 -0.67 -5.76 0.65 2.12 6.11 -1.61 -7.32 -0.06 2.21 7.84
Bud Site 1.20 -0.50 1.51 0.50 2.08 -0.66 -3.54 -1.66 -0.97 3.15 -1.78 -4.46 -2.18 -0.21 3.77
Cell Periphery 2.98 3.32 1.60 -2.53 -3.29 1.04 -1.73 -1.35 -4.59 1.19 1.13 -0.52 -2.26 -3.52 -1.63
Cytoplasm -1.56 -2.49 -0.25 1.52 2.95 1.67 0.88 1.82 0.65 4.56 0.96 0.65 1.64 1.62 5.41
Cytoplasmic Foci 0.13 -7.99 4.33 6.98 10.36 -5.15 -5.78 -1.88 3.27 5.02 -7.08 -10.93 -0.38 6.83 10.85
Eisosomes 1.32 2.89 -0.43 -2.72 -5.63 -2.10 -2.37 -3.07 -1.49 1.92 -3.19 -2.07 -5.43 -3.14 -1.16
Endoplasmic Reticulum 4.85 3.96 4.48 -1.85 1.80 0.57 -3.17 -1.80 -2.99 2.06 3.30 -1.37 0.99 -3.06 2.58
Endosome 2.56 -0.93 5.90 8.59 10.67 -3.96 -5.36 1.59 5.62 6.13 -4.83 -8.90 4.62 10.04 11.54
Golgi 9.99 6.45 10.23 1.37 7.96 -5.51 -5.21 -11.48 -7.60 -2.50 1.55 -5.78 -3.38 -5.81 3.66
Lipid Particles -1.43 -1.47 1.73 5.85 4.17 -0.62 -1.77 3.61 3.47 3.10 -1.88 -2.60 3.71 5.92 4.56
Mitochondria -11.53 -1.10 -13.80 -5.80 -16.94 6.29 10.32 9.54 3.18 -6.06 3.89 15.69 2.86 -1.27 -14.68
Mitotic Spindle 1.67 -5.79 1.79 0.55 6.45 -2.46 -4.89 -4.35 -1.03 4.46 -2.03 -7.96 -3.04 -0.65 7.72
None 3.63 0.90 -2.10 -5.98 -3.07 1.79 0.51 0.49 -14.02 -0.13 1.66 0.50 0.14 -12.86 -2.19
Nuclear Periphery 2.03 -2.07 1.94 -1.99 5.58 0.71 -4.44 -2.80 -3.09 3.30 2.00 -6.39 -0.36 -3.54 6.38
Nuclear Periphery Foci 1.88 -2.42 3.43 2.03 4.49 -1.28 -4.76 -0.86 0.98 4.49 -1.35 -5.22 1.54 2.10 5.55
Nucleolus -0.81 -0.42 0.97 1.78 1.37 -1.68 -2.52 -0.81 1.47 1.83 -2.33 -2.16 -0.25 2.27 2.06
Nucleus -0.63 -0.43 1.15 1.95 1.47 -0.06 -0.92 2.02 1.51 1.95 -0.98 -1.25 2.28 2.46 2.37
Peroxisomes -6.21 -4.10 -2.18 4.91 2.80 -4.24 -5.40 3.55 6.92 6.19 -8.44 -7.09 -0.99 7.55 6.69
Vacuole 5.41 0.89 5.60 1.05 7.15 -3.99 -5.94 -6.42 -3.48 3.02 0.99 -7.65 -0.89 -2.17 7.39
Vacuole Periphery 6.59 1.14 5.97 -3.97 7.07 -1.52 -5.32 -11.14 -10.13 0.51 4.38 -7.54 -4.39 -10.57 5.82
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging; expression of human homolog AFG3L2 can complement yeast yta12 afg3 double mutant
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Afg3

Afg3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Afg3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available