Standard name | |
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Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 2 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 2 | 4 | – | 4 | 3 | 24 | 10 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 5 | 7 | 9 | 17 | 8 | 8 | – | 11 | 14 | 6 | 13 | 22 | 34 | 54 | 6 | 18 | 13 | 0 | 2 | 2 | 1 | 2 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 7 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 0 | 4 | 1 | 0 | 0 | 1 | 1 | – | 0 | 1 | 0 | 2 | 0 | 3 | 4 | 5 | 5 | 4 | 0 | 10 | 2 | 3 | 1 | 4 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 0 | 3 | 0 | 0 | 0 | 1 |
Mitochondria | 4 | 8 | 0 | 3 | 1 | 19 | 15 | – | 27 | 38 | 76 | 26 | 0 | 0 | 0 | 5 | 5 | 2 | 2 | 4 | 1 | 0 | 2 | 7 |
Nucleus | 132 | 414 | 203 | 191 | 245 | 228 | 234 | – | 242 | 242 | 247 | 280 | 205 | 243 | 197 | 124 | 178 | 124 | 128 | 443 | 194 | 139 | 165 | 214 |
Nuclear Periphery | 1 | 0 | 1 | 1 | 4 | 2 | 5 | – | 4 | 1 | 3 | 5 | 3 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 40 | 101 | 13 | 22 | 26 | 39 | 58 | – | 90 | 78 | 129 | 92 | 21 | 53 | 27 | 15 | 19 | 15 | 17 | 75 | 20 | 25 | 30 | 66 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 3 | 5 | 1 | 0 | 2 | – | 7 | 4 | 1 | 3 | 5 | 4 | 8 | 0 | 1 | 1 | 2 | 3 | 1 | 1 | 2 | 7 |
Vac/Vac Membrane | 1 | 7 | 4 | 3 | 6 | 0 | 1 | – | 2 | 6 | 1 | 2 | 1 | 2 | 5 | 5 | 13 | 6 | 4 | 12 | 8 | 10 | 12 | 15 |
Unique Cell Count | 146 | 476 | 219 | 212 | 283 | 270 | 290 | 311 | 312 | 332 | 371 | 241 | 307 | 277 | 148 | 224 | 155 | 158 | 565 | 241 | 189 | 221 | 328 | |
Labelled Cell Count | 178 | 544 | 232 | 237 | 300 | 299 | 331 | 389 | 389 | 492 | 434 | 260 | 341 | 301 | 162 | 252 | 172 | 158 | 565 | 241 | 189 | 221 | 328 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 10.2 | 9.0 | 8.1 | 8.5 | 6.8 | 7.0 | – | 6.2 | 6.3 | 5.7 | 6.4 | 8.9 | 9.1 | 8.7 | 11.1 | 11.2 | 10.8 | 8.0 | 8.5 | 8.9 |
Std Deviation (1e-4) | 1.5 | 2.0 | 1.8 | 1.7 | 1.7 | 1.3 | 1.6 | – | 1.3 | 1.5 | 1.3 | 1.6 | 1.7 | 1.9 | 2.3 | 2.1 | 2.6 | 2.5 | 1.6 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.09 | -0.41 | -0.36 | – | -0.55 | -0.51 | -0.67 | -0.49 | -0.02 | 0.01 | -0.05 | 0.29 | 0.31 | 0.25 | -0.18 | -0.08 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | 1.5 | -0.1 | -0.3 | – | 0.2 | 0.8 | -1.1 | 0.2 | 2.6 | 3.3 | 5.5 | 0.4 | 2.2 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.0 | 3.4 | – | 4.5 | 5.4 | 7.6 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.0 | -2.2 | -2.8 | -3.8 | – | -4.6 | -4.7 | -5.4 | -5.2 | -2.6 | -4.3 | -6.0 | -2.7 | -4.0 | -3.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.7 | 1.3 | 3.0 | 4.5 | – | 6.6 | 5.7 | 8.6 | 5.8 | 1.1 | 3.9 | 1.5 | 1.5 | 1.0 | 1.4 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3097 | 2.6611 | 2.4263 | 2.3446 | 1.6706 | 2.0484 | 3.162 | 3.2879 | 3.078 | 2.9233 | 2.6041 | 2.8934 | 4.2071 | 4.3086 | 3.9612 | 3.8101 | 3.6789 | 3.7307 |
Actin | 0.0204 | 0.0033 | 0.0049 | 0 | 0.0017 | 0 | 0.0124 | 0 | 0.0007 | 0 | 0.0017 | 0.0004 | 0.0036 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0006 | 0.0028 | 0.0008 | 0 | 0.0009 | 0 | 0.0012 | 0.0007 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0038 | 0.0005 |
Bud Periphery | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0009 | 0.0011 | 0 | 0.0009 | 0 | 0.0004 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0.0002 |
Bud Site | 0.0006 | 0.0003 | 0.0012 | 0 | 0.0003 | 0.0005 | 0.0008 | 0 | 0.0006 | 0 | 0.0007 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0005 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0146 | 0.0001 | 0.0018 | 0 | 0.0017 | 0.0001 | 0.0038 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0011 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.008 | 0.0024 | 0.0186 | 0 | 0.0122 | 0 | 0.0061 | 0 | 0.0006 | 0 | 0.0013 | 0.0002 | 0.001 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0045 | 0.0003 | 0.0007 | 0 | 0.0022 | 0 | 0.001 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 |
Endosome | 0.0131 | 0.0043 | 0.0039 | 0 | 0.0102 | 0.0001 | 0.0045 | 0 | 0.0028 | 0 | 0.0007 | 0.0007 | 0.0018 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Golgi | 0.0055 | 0.0078 | 0.0014 | 0 | 0.0023 | 0 | 0.0026 | 0 | 0.0005 | 0 | 0.0012 | 0.0001 | 0.0008 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0068 | 0.0039 | 0.0063 | 0 | 0.0252 | 0 | 0.0087 | 0 | 0.0016 | 0 | 0.0105 | 0.0003 | 0.0058 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0024 | 0.0176 | 0.0112 | 0.0001 | 0.0019 | 0.0002 | 0.0102 | 0 | 0.0062 | 0 | 0.0133 | 0.0007 | 0.0011 | 0 | 0.0003 | 0 | 0.0002 | 0.0017 |
None | 0.0032 | 0.0001 | 0.0021 | 0 | 0.0023 | 0 | 0.0056 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0214 | 0.0003 | 0.0036 | 0.0002 | 0.002 | 0.0002 | 0.0158 | 0.0003 | 0.0036 | 0.0002 | 0.001 | 0.0025 | 0.0051 | 0.0012 | 0.003 | 0.0002 | 0.0036 | 0.0002 |
Nucleolus | 0.0367 | 0.0345 | 0.0349 | 0.0283 | 0.1726 | 0.1438 | 0.0362 | 0.028 | 0.026 | 0.0138 | 0.2001 | 0.1055 | 0.0347 | 0.0292 | 0.022 | 0.0207 | 0.1996 | 0.0954 |
Nucleus | 0.8357 | 0.9225 | 0.8714 | 0.9703 | 0.7529 | 0.8489 | 0.8738 | 0.9712 | 0.9511 | 0.9858 | 0.7532 | 0.8826 | 0.937 | 0.9676 | 0.9711 | 0.978 | 0.7776 | 0.9002 |
Peroxisomes | 0.0167 | 0.0014 | 0.0061 | 0 | 0.0014 | 0 | 0.0038 | 0 | 0.0002 | 0 | 0.0079 | 0.0001 | 0.0028 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0071 | 0.0006 | 0.03 | 0.0005 | 0.0092 | 0.001 | 0.0051 | 0.0004 | 0.0012 | 0.0001 | 0.0054 | 0.0047 | 0.002 | 0.0017 | 0.003 | 0.001 | 0.0142 | 0.0011 |
Vacuole | 0.0012 | 0.0002 | 0.0004 | 0 | 0.0004 | 0.0008 | 0.0044 | 0 | 0.0015 | 0 | 0.0004 | 0.0003 | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 |
Vacuole Periphery | 0.0012 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.002 | 0 | 0.0006 | 0 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.4341 | 11.9474 | 13.4755 | 14.4326 | 21.1403 | 17.0976 | 18.2408 | 17.1217 | 14.0444 | 16.6551 |
Translational Efficiency | 1.4235 | 1.7191 | 1.2202 | 1.2725 | 0.9598 | 1.1548 | 1.0491 | 1.0572 | 1.1334 | 1.1208 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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