Standard name
Human Ortholog
Description Protein involved in 3' mRNA processing; interacts with Ref2p; APCC(Cdh1) substrate; potential Cdc28p substrate
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0.05 0.06 0.05 0 0 0 0.05 0 0.06 0.07 0 0 0 0 0 0 0.06 0 0 0.05 0 0
Bud Neck 0 – 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.09 0.1 0.06 0
Bud Site 0 – 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.82 – 0.89 0.83 0.86 0.79 0.87 0.81 0.78 0.79 0.73 0.69 0.95 0.99 1.0 0.78 0.78 0.81 0.69 0.65 0.65 0.66 0.76 0.8
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 – 0.05 0.07 0.07 0.15 0.17 0.26 0.32 0.33 0.42 0.42 0.06 0 0.05 0 0 0 0.06 0 0.09 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0.06 0 0 0.06 0 0 0 0 0 0 0.09 0.11 0.09 0 0 0.05 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 – 5 7 2 8 1 2 1 2 0 1 0 0 0 16 9 9 2 0 6 1 5 8
Bud 1 – 10 22 18 15 10 36 20 23 24 37 3 0 2 15 7 9 4 1 3 2 5 8
Bud Neck 1 – 6 19 17 22 10 23 6 17 11 10 0 0 0 6 4 3 6 3 16 5 10 2
Bud Site 1 – 8 23 10 28 15 10 8 7 0 7 3 0 0 11 1 7 – – – – – –
Cell Periphery 0 – 1 1 1 2 6 2 1 7 6 5 0 1 0 17 8 10 0 0 2 0 0 1
Cytoplasm 42 – 182 300 323 370 556 657 310 423 301 393 111 149 175 453 199 293 48 23 126 34 140 177
Endoplasmic Reticulum 0 – 0 0 1 0 0 1 0 0 0 0 0 1 0 35 11 20 0 0 0 0 0 2
Endosome 1 – 0 0 1 0 0 0 0 0 0 0 0 0 0 23 8 7 0 1 2 1 2 1
Golgi 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0
Mitochondria 4 – 11 25 25 70 110 211 128 176 175 239 7 0 8 25 10 5 4 0 17 1 6 6
Nucleus 0 – 0 2 2 1 3 5 2 4 2 2 0 0 0 2 3 5 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 – 1 3 2 2 3 6 2 5 2 4 0 0 0 1 2 0 0 0 0 0 0 0
Peroxisomes 3 – 1 5 1 5 3 1 0 0 3 5 1 0 0 30 11 13 0 0 0 0 0 0
SpindlePole 1 – 7 16 16 29 24 25 22 22 17 24 3 0 0 54 28 34 0 1 9 1 1 2
Vac/Vac Membrane 0 – 0 0 0 1 0 1 2 4 1 1 0 0 1 12 1 8 1 1 2 0 4 2
Unique Cell Count 51 204 362 375 469 637 814 397 538 414 568 117 150 175 579 254 363 70 36 196 53 184 222
Labelled Cell Count 56 232 423 420 553 741 980 502 690 542 729 128 151 186 703 302 424 70 36 196 53 184 222


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 – 3.2 3.0 3.2 3.0 2.8 2.9 2.5 2.5 2.5 2.3 4.0 4.5 4.0 5.8 5.7 6.3 4.1 4.0 5.2
Std Deviation (1e-4) 0.6 – 0.9 0.8 0.8 0.7 0.9 1.0 0.9 1.0 0.6 0.8 0.6 1.2 1.2 1.3 1.5 1.5 0.7 1.3 2.7
Intensity Change (Log2) – – – -0.07 0.01 -0.09 -0.19 -0.1 -0.37 -0.33 -0.36 -0.44 0.33 0.51 0.33 0.88 0.84 1.0 0.37 0.34 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.7 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 -1.6 -1.2 -1.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -1.4
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.0 -1.1 -3.2 -0.7 -2.9 -3.4 -3.3 -4.7 -5.6 1.7 3.8 4.5 -3.4 -3.1 -2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.6 0 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -0.6 -0.7 -2.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 3.0 2.6 2.3
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 2.7 3.0 2.6
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3095 0.1711 0.1762 0.7656 0.3346 0.1822 0.5878 1.3537 1.5106 1.6155 1.1257 1.3389 -1.2481 -0.2139 -0.3081 0.0794 -0.1052 -0.2192
Actin 0.0249 0.0012 0.0041 0.0363 0.0175 0.0062 0.016 0.0027 0.0089 0.0004 0.0021 0.0006 0.0056 0.0001 0.0041 0.0002 0.0086 0.0004
Bud 0.0017 0.0043 0.0304 0.0086 0.0042 0.0007 0.0016 0.007 0.021 0.0102 0.0004 0.0022 0.0007 0.0006 0.013 0.0002 0.0003 0.0004
Bud Neck 0.0243 0.004 0.0073 0.0544 0.2618 0.3439 0.0252 0.0034 0.0031 0.0003 0.3667 0.4072 0.0219 0.0018 0.0108 0.0662 0.2632 0.3275
Bud Periphery 0.0058 0.0094 0.0762 0.0221 0.0066 0.0009 0.0042 0.0086 0.05 0.0247 0.0004 0.0067 0.0008 0.0005 0.0443 0.0004 0.0006 0.0003
Bud Site 0.0415 0.0659 0.0298 0.0197 0.0167 0.0008 0.0338 0.0477 0.0283 0.004 0.0008 0.0027 0.0236 0.0571 0.0258 0.0004 0.0034 0.0025
Cell Periphery 0.0015 0.002 0.0021 0.002 0.0011 0.0002 0.0009 0.0011 0.0007 0.0005 0.0002 0.0001 0.0003 0.0002 0.0009 0.0002 0.0005 0.0001
Cytoplasm 0.1226 0.257 0.3386 0.4348 0.297 0.1944 0.1018 0.2862 0.3884 0.4589 0.3139 0.1852 0.0288 0.1225 0.109 0.2224 0.0857 0.0493
Cytoplasmic Foci 0.0664 0.0442 0.0348 0.0397 0.059 0.0473 0.0654 0.0162 0.0304 0.0414 0.0543 0.0214 0.0482 0.0039 0.0123 0.0152 0.1133 0.0152
Eisosomes 0.0003 0.0004 0.0005 0.0009 0.0009 0.0001 0.0004 0.0001 0.0001 0 0 0 0.0003 0.0001 0.0006 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0032 0.0051 0.0041 0.0083 0.0048 0.001 0.0022 0.001 0.0024 0.0017 0.0009 0.0004 0.0002 0.0003 0.0037 0.0006 0.0004 0.0002
Endosome 0.011 0.0048 0.0065 0.0179 0.0099 0.0029 0.016 0.0012 0.0084 0.0168 0.0018 0.0013 0.0011 0.0003 0.0054 0.0012 0.0026 0.0007
Golgi 0.0028 0.0006 0.001 0.0057 0.0041 0.0007 0.006 0.0004 0.0026 0.0032 0.0007 0.0002 0.0016 0.0001 0.0009 0.0001 0.0015 0.0002
Lipid Particles 0.005 0.0079 0.0022 0.0112 0.0042 0.002 0.0165 0.0013 0.0029 0.0045 0.0001 0.0004 0.0095 0.0001 0.0032 0.0001 0.0104 0.0053
Mitochondria 0.0023 0.0009 0.003 0.0295 0.0102 0.0007 0.0119 0.0004 0.0061 0.0021 0.0004 0.0004 0.0017 0.0001 0.0039 0.0002 0.0004 0.0001
None 0.6522 0.5762 0.4335 0.262 0.2663 0.3914 0.6647 0.6142 0.426 0.4142 0.228 0.3635 0.8386 0.7914 0.7376 0.6886 0.4898 0.5938
Nuclear Periphery 0.007 0.0014 0.0011 0.0113 0.0008 0.0006 0.0092 0.0002 0.0023 0.0008 0.0002 0.0002 0.0001 0.0001 0.0013 0.0002 0.0003 0.0001
Nucleolus 0.0022 0.0014 0.0014 0.0061 0.0033 0.0007 0.0026 0.0034 0.001 0.0003 0.0003 0.0004 0.0016 0.0005 0.0054 0.0002 0.0049 0.0012
Nucleus 0.0033 0.0034 0.0122 0.0071 0.0027 0.0006 0.0054 0.001 0.0034 0.0015 0.0005 0.0006 0.0004 0.0009 0.0053 0.0005 0.0012 0.0003
Peroxisomes 0.0113 0.0009 0.0027 0.005 0.0079 0.0011 0.0057 0.0007 0.0072 0.0056 0.001 0.0033 0.0057 0.0001 0.0064 0.0003 0.0068 0.001
Punctate Nuclear 0.0047 0.0057 0.0042 0.0106 0.018 0.0031 0.006 0.0017 0.0029 0.0013 0.0267 0.0028 0.0089 0.0185 0.0033 0.0017 0.005 0.001
Vacuole 0.0055 0.003 0.0038 0.0052 0.0024 0.0008 0.0034 0.0015 0.0034 0.0058 0.0006 0.0004 0.0004 0.0009 0.0024 0.0009 0.0007 0.0002
Vacuole Periphery 0.0006 0.0003 0.0005 0.0015 0.0007 0.0001 0.0012 0.0001 0.0006 0.0019 0.0001 0.0001 0 0 0.0004 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.001 14.6213 7.7895 9.0428 12.7782 5.8682 23.1231 15.7344 10.1497 14.8719
Translational Efficiency 1.3488 1.5515 1.148 0.7896 1.0704 2.2122 0.8371 1.0003 1.0688 1.0113

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1469 1274 355 986 804 1918 37 235 2273 3192 392 1221

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 610.03 657.75 895.91 859.58 617.28 704.36 957.94 861.10 612.59 685.76 901.76 859.87
Standard Deviation 81.53 95.74 116.21 110.98 99.27 83.07 94.31 108.14 88.28 91.25 115.75 110.44
Intensity Change Log 2 — 0.108659 0.554474 0.494752 — 0.190388 0.634010 0.480256 — 0.150344 0.595025 0.487479

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000205 0.000610 0.001483 0.003007 0.000159 0.000307 0.000484 0.001624 0.000189 0.000428 0.001388 0.002741
Bud Neck 0.098191 0.096791 0.063999 0.105093 0.095555 0.156026 0.051581 0.077856 0.097259 0.132384 0.062827 0.099851
Bud Site 0.074594 0.075019 0.097459 0.269543* 0.036674 0.064210 0.068332 0.302248* 0.061181 0.068524 0.094709 0.275837*
Cell Periphery 0.000993 0.000376 0.000423 0.000335 0.000205 0.000813 0.000487 0.000227 0.000714 0.000639 0.000429 0.000314
Cytoplasm 0.461403* 0.359402* 0.316783* 0.298624* 0.467188* 0.339721* 0.283998* 0.296395* 0.463450* 0.347576* 0.313689* 0.298195*
Cytoplasmic Foci 0.158811 0.230026* 0.038426 0.034735 0.176486 0.154854 0.056462 0.040439 0.165063 0.184857* 0.040128 0.035833
Eisosomes 0.000247 0.000151 0.000112 0.000113 0.000113 0.000274 0.000218 0.000087 0.000200 0.000225 0.000122 0.000108
Endoplasmic Reticulum 0.000508 0.000773 0.006456 0.002760 0.000858 0.000795 0.004976 0.002008 0.000632 0.000786 0.006317 0.002615
Endosome 0.012444 0.035837 0.009105 0.011417 0.013821 0.027173 0.015512 0.003566 0.012931 0.030631 0.009710 0.009906
Golgi 0.002173 0.006879 0.000393 0.005507 0.001462 0.004060 0.000433 0.000440 0.001921 0.005185 0.000396 0.004532
Lipid Particles 0.005281 0.008871 0.003421 0.000986 0.009401 0.009727 0.004251 0.001059 0.006739 0.009385 0.003499 0.001000
Mitochondria 0.003520 0.009773 0.000499 0.001618 0.002859 0.008281 0.000194 0.002358 0.003286 0.008877 0.000470 0.001760
Mitotic Spindle 0.000686 0.004400 0.005067 0.023515 0.001482 0.004315 0.000267 0.017150 0.000968 0.004349 0.004614 0.022290
None 0.011676 0.005704 0.002144 0.003527 0.006463 0.003103 0.000758 0.002375 0.009832 0.004141 0.002013 0.003305
Nuclear Periphery 0.000289 0.000370 0.001110 0.000508 0.000774 0.000367 0.001106 0.000445 0.000460 0.000368 0.001110 0.000496
Nuclear Periphery Foci 0.000957 0.000810 0.004527 0.000999 0.000804 0.000308 0.002545 0.001195 0.000903 0.000508 0.004340 0.001037
Nucleolus 0.002521 0.004020 0.000603 0.000330 0.002513 0.001946 0.000599 0.000154 0.002519 0.002774 0.000602 0.000296
Nucleus 0.108668 0.067132 0.325994* 0.084788 0.127408 0.127078 0.303987* 0.069532 0.115296 0.103152 0.323917* 0.081852
Peroxisomes 0.006738 0.021665 0.005947 0.009573 0.006229 0.012288 0.005243 0.011242 0.006558 0.016030 0.005880 0.009895
Vacuole 0.048678 0.069174 0.115676 0.141994 0.048612 0.082468 0.198118 0.169202 0.048655 0.077162 0.123457 0.147231
Vacuole Periphery 0.001417 0.002218 0.000375 0.001028 0.000933 0.001885 0.000449 0.000398 0.001246 0.002018 0.000382 0.000907

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.41 -2.92 -3.45 -2.52 -0.57 -2.86 -3.61 -2.81 -2.41 -1.66 -3.28 -3.03 -3.91 -3.28 -0.58
Bud Neck 0.33 5.68 6.18 5.73 -0.88 -5.88 2.93 5.21 12.69 -0.16 -5.41 6.30 7.61 13.82 -0.70
Bud Site 0.09 0.05 -7.00 -7.02 -5.30 -5.36 -0.47 -5.72 -4.17 -3.37 -1.71 -1.12 -9.72 -9.03 -5.97
Cell Periphery 2.65 2.41 3.30 2.11 4.18 -5.86 -2.86 0.52 6.15 3.20 0.47 1.82 3.26 6.05 4.86
Cytoplasm 9.13 10.63 13.23 4.31 -0.29 10.29 4.60 7.65 1.74 -1.08 14.51 11.90 15.50 4.42 -0.62
Cytoplasmic Foci -9.23 22.54 25.77 30.28 1.45 2.87 10.01 20.13 23.75 1.28 -3.78 26.95 32.52 38.35 1.76
Eisosomes 3.34 4.20 4.83 3.66 1.32 -8.67 -3.16 1.05 9.95 3.65 -1.20 3.31 4.80 9.72 2.31
Endoplasmic Reticulum -2.49 -11.15 -5.13 -4.53 6.12 0.32 -4.09 -5.07 -5.35 2.43 -1.60 -11.65 -6.01 -5.61 6.64
Endosome -10.09 1.82 4.56 12.41 1.37 -5.96 0.39 6.94 13.64 2.45 -11.67 2.14 6.54 16.22 2.09
Golgi -5.14 7.95 -0.29 3.52 -2.38 -5.72 4.65 5.51 9.59 0.55 -7.11 9.28 -0.12 3.78 -2.35
Lipid Particles -3.09 1.96 7.12 6.80 5.93 -0.20 2.89 5.22 9.68 3.02 -3.09 3.80 8.40 12.16 6.35
Mitochondria -3.84 4.43 3.29 5.74 -2.08 -5.71 4.68 1.73 6.33 -1.10 -6.32 5.78 3.92 9.69 -2.22
Mitotic Spindle -3.35 -2.24 -6.17 -4.17 -2.78 -2.24 1.32 -2.38 -1.91 -2.59 -4.26 -2.10 -6.47 -4.81 -3.18
None 3.51 7.20 5.92 2.02 -2.03 2.01 3.69 2.72 1.86 -2.44 4.99 7.63 6.15 1.77 -2.18
Nuclear Periphery -1.08 -11.22 -10.25 -9.73 1.26 2.00 -1.95 -1.20 -7.00 1.48 1.03 -8.63 -7.62 -12.21 1.99
Nuclear Periphery Foci 0.72 -5.63 -1.64 -3.59 5.29 3.96 -3.87 -4.25 -7.02 1.95 2.84 -6.03 -3.21 -8.88 5.36
Nucleolus -1.91 6.40 7.02 5.17 2.45 1.13 3.85 4.32 7.79 1.58 -0.48 7.23 8.02 7.71 2.96
Nucleus 8.08 -14.29 -4.96 -10.67 11.30 0.19 -3.91 -0.36 -0.49 3.74 2.86 -14.51 -4.24 -6.25 12.06
Peroxisomes -7.56 1.07 0.80 7.78 -0.51 -4.71 0.87 0.26 3.58 -0.64 -8.58 1.15 0.80 8.26 -0.62
Vacuole -5.36 -10.64 -24.18 -21.20 -8.00 -7.63 -4.99 -13.66 -11.98 -1.53 -9.86 -11.69 -27.76 -24.15 -8.40
Vacuole Periphery -1.50 2.12 1.75 6.74 -1.05 -4.44 3.32 4.45 7.55 0.73 -2.24 2.72 2.30 8.61 -0.79
Standard name
Human Ortholog
Description Protein involved in 3' mRNA processing; interacts with Ref2p; APCC(Cdh1) substrate; potential Cdc28p substrate
Localization
Cell Percentages cytoplasm (57%), bud neck (11%), mixed (10%)
Cell Cycle Regulation Yes
cytoplasm - S/G2 (0.00025)
Subcompartmental Group N/A

Fir1

Fir1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fir1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available