Standard name
Human Ortholog
Description Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.08 0 0 0 0 0 0
Cytoplasm 0.97 0.95 0.96 0.96 0.97 0.93 0.91 0.87 0.82 0.83 0.74 0.75 1.0 1.0 0.98 0.9 0.9 0.93 0.79 0.85 0.75 0.94 0.84 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.15 0.21 0.27 0.43 0.42 0.44 0.51 0 0 0 0 0 0 0 0 0.12 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 2 2 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 10 6 6 0 7 8
Bud 3 5 6 7 3 5 12 13 14 6 10 16 0 0 10 1 0 0 10 1 3 1 4 7
Bud Neck 0 3 6 4 1 2 3 5 1 0 0 1 0 0 0 0 1 0 6 4 6 1 4 4
Bud Site 0 0 1 1 1 0 2 2 4 1 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 5 10 5 2 12 9 8 4 9 6 0 9 14 10 15 13 0 2 3 0 1 2
Cytoplasm 193 248 277 302 331 364 613 564 383 327 265 345 189 398 417 122 137 156 166 232 215 167 209 280
Endoplasmic Reticulum 0 2 2 1 0 4 0 1 1 1 2 1 0 1 1 11 9 5 0 0 4 0 2 3
Endosome 0 0 0 0 0 0 0 0 0 0 1 2 0 0 2 1 1 1 3 1 0 0 3 2
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 4 2 1 1 0 0 3 1
Mitochondria 0 9 5 20 12 60 143 173 202 165 158 234 1 0 15 4 2 5 4 10 35 4 7 10
Nucleus 0 1 0 0 0 0 2 5 1 0 2 0 0 1 2 1 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 1 0 1 0 0 1 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2 0 0 0 1 2 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 2 0 1 4 2 2 1 6 5 0 0 0 2 0 2 0 0 1 0 0 2
Vac/Vac Membrane 0 0 0 0 0 1 3 1 0 1 3 4 0 0 0 3 9 4 2 6 5 1 1 2
Unique Cell Count 199 261 288 313 341 390 670 651 468 395 358 458 189 399 425 136 153 168 210 274 287 179 249 331
Labelled Cell Count 203 273 304 351 354 440 795 776 619 506 460 617 190 409 463 158 178 188 210 274 287 179 249 331


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 3.8 3.4 3.3 3.7 3.5 3.3 3.3 2.9 2.9 3.1 2.7 4.5 4.7 4.2 7.0 7.4 7.9 4.5 4.4 5.3
Std Deviation (1e-4) 0.8 0.6 0.4 1.0 1.2 1.3 1.3 0.8 0.8 0.7 2.1 0.8 1.2 1.0 0.9 1.6 2.3 2.6 0.7 1.5 1.6
Intensity Change (Log2) – – – -0.05 0.11 0.01 -0.06 -0.06 -0.26 -0.25 -0.17 -0.33 0.38 0.46 0.3 1.02 1.12 1.21 0.39 0.37 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7159 0.9879 0.9408 0.7242 0.5457 0.8808 0.689 0.6188 0.4611 0.1697 0.4399 0.7498 0.8647 1.7434 0.963 1.9731 1.2522 1.4292
Actin 0.1034 0.001 0.0297 0.0144 0.0378 0.0123 0.0659 0.0007 0.0043 0.0411 0.0433 0.0052 0.0024 0.0029 0.0016 0.0023 0.0008 0.0007
Bud 0.008 0.0021 0.0008 0.0005 0.0041 0.0082 0.001 0.0011 0.0007 0.0003 0.0019 0.001 0.0013 0.0005 0.0039 0.0001 0.0006 0.0001
Bud Neck 0.0167 0.0035 0.0023 0.0034 0.0008 0.0123 0.002 0.0001 0.0012 0.0003 0.0024 0.0007 0.0009 0.0004 0.0001 0.0001 0.0003 0.0052
Bud Periphery 0.0102 0.0047 0.0023 0.0008 0.0091 0.0049 0.0024 0.0012 0.0031 0.0006 0.0045 0.0048 0.003 0.0004 0.0043 0.0003 0.0014 0.0002
Bud Site 0.0158 0.0079 0.0058 0.0046 0.0016 0.0018 0.0024 0.0052 0.0025 0.0011 0.0026 0.0029 0.007 0.0291 0.0098 0.0001 0.0004 0.0001
Cell Periphery 0.0015 0.0016 0.0006 0.0006 0.0007 0.0004 0.0005 0.0003 0.0003 0.0001 0.0003 0.0002 0.0006 0.0003 0.0004 0.0002 0.0004 0.0002
Cytoplasm 0.1832 0.2877 0.2557 0.2257 0.2393 0.34 0.1335 0.2319 0.1972 0.1815 0.213 0.3277 0.1541 0.1243 0.1707 0.1189 0.2666 0.2448
Cytoplasmic Foci 0.0424 0.0122 0.0131 0.0117 0.0191 0.0263 0.0346 0.0094 0.0089 0.0143 0.0113 0.0069 0.0065 0.0037 0.0088 0.0035 0.0092 0.003
Eisosomes 0.002 0.0003 0.0005 0.0003 0.0004 0.0001 0.0019 0.0001 0.0002 0.0003 0.0012 0.0001 0.0009 0.0002 0.0003 0.0004 0.0002 0.0001
Endoplasmic Reticulum 0.0069 0.0017 0.0031 0.0023 0.0035 0.0011 0.0042 0.0008 0.0012 0.0025 0.0026 0.0018 0.0027 0.0006 0.0021 0.0007 0.002 0.0012
Endosome 0.0308 0.0023 0.0094 0.0253 0.0195 0.0054 0.0165 0.0006 0.0017 0.0065 0.0093 0.0048 0.0035 0.0005 0.002 0.0005 0.0024 0.0005
Golgi 0.0178 0.0003 0.004 0.0145 0.0069 0.0023 0.0054 0.0002 0.0004 0.0052 0.0081 0.001 0.001 0.0006 0.0006 0.0003 0.0003 0.0001
Lipid Particles 0.0274 0.0005 0.0037 0.0125 0.0332 0.0062 0.011 0.0002 0.0023 0.0124 0.0093 0.0028 0.0011 0.0002 0.0005 0.0001 0.0005 0.0002
Mitochondria 0.0233 0.0007 0.0108 0.0139 0.0034 0.0052 0.005 0.0002 0.0004 0.0026 0.0025 0.0009 0.0011 0.0002 0.0014 0.0003 0.0004 0.0001
None 0.4437 0.6641 0.6301 0.6511 0.5647 0.5653 0.6701 0.7445 0.7678 0.6804 0.6373 0.6272 0.8089 0.8349 0.789 0.8713 0.7112 0.7427
Nuclear Periphery 0.0106 0.0003 0.0016 0.0011 0.0126 0.0008 0.0138 0.0002 0.0006 0.0033 0.004 0.0057 0.0008 0.0001 0.0006 0.0002 0.0004 0.0001
Nucleolus 0.0025 0.0011 0.002 0.0027 0.001 0.0008 0.0011 0.0003 0.0001 0.0003 0.0077 0.0003 0.0004 0.0001 0.0003 0.0001 0.0002 0
Nucleus 0.0035 0.0007 0.0083 0.0013 0.0037 0.0011 0.0023 0.0008 0.0005 0.0007 0.0038 0.0014 0.0007 0.0003 0.0006 0.0002 0.0006 0.0002
Peroxisomes 0.0338 0.0004 0.008 0.002 0.0182 0.0018 0.0107 0.0003 0.0023 0.0404 0.0063 0.0009 0.0011 0.0004 0.0011 0.0002 0.0002 0.0001
Punctate Nuclear 0.0092 0.0019 0.005 0.0009 0.0188 0.0021 0.0136 0.0013 0.0031 0.0049 0.0259 0.0027 0.0006 0.0003 0.001 0.0002 0.0006 0.0002
Vacuole 0.0052 0.0047 0.0023 0.0089 0.0012 0.001 0.0011 0.0005 0.0009 0.0008 0.0019 0.0006 0.0014 0.0003 0.0007 0.0002 0.0012 0.0002
Vacuole Periphery 0.0021 0.0005 0.0006 0.0016 0.0006 0.0004 0.001 0 0.0002 0.0004 0.0008 0.0004 0.0003 0 0.0001 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.049 2.137 5.6634 9.0663 4.8705 3.0718 4.7844 8.3334 8.3736 6.5181
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1419 229 1918 1149 1665 796 915 1851 3084 1025 2833 3000

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 643.09 704.05 956.14 667.46 697.57 669.58 763.20 847.02 672.50 677.28 893.82 778.25
Standard Deviation 69.77 83.83 110.67 78.45 84.26 88.22 94.35 99.88 82.53 88.43 138.95 127.01
Intensity Change Log 2 — 0.130657 0.572201 0.053661 — -0.059082 0.129723 0.280058 — 0.035050 0.358911 0.175879

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000333 0.000760 0.000523 0.000222 0.000180 0.000749 0.000488 0.000037 0.000250 0.000752 0.000512 0.000108
Bud Neck 0.044206 0.058077 0.014690 0.010509 0.015513 0.024439 0.006245 0.010311 0.028715 0.031954 0.011962 0.010387
Bud Site 0.021978 0.026234 0.046455 0.008529 0.005237 0.015755 0.015349 0.009041 0.012940 0.018096 0.036409 0.008845
Cell Periphery 0.000497 0.000233 0.000175 0.000353 0.000153 0.000470 0.000189 0.000054 0.000311 0.000417 0.000180 0.000168
Cytoplasm 0.536414* 0.294207* 0.634254* 0.428787* 0.561934* 0.421042* 0.637498* 0.735781* 0.550192* 0.392706* 0.635302* 0.618202*
Cytoplasmic Foci 0.212557* 0.354575* 0.033580 0.056872 0.222121* 0.283425* 0.054942 0.009852 0.217721* 0.299321* 0.040480 0.027861
Eisosomes 0.000700 0.000348 0.000048 0.000104 0.000192 0.000537 0.000068 0.000015 0.000426 0.000495 0.000054 0.000049
Endoplasmic Reticulum 0.000626 0.000474 0.001032 0.001532 0.000746 0.000687 0.001482 0.000124 0.000691 0.000639 0.001177 0.000663
Endosome 0.018564 0.056594 0.002596 0.005473 0.011120 0.022119 0.003518 0.000333 0.014545 0.029821 0.002894 0.002301
Golgi 0.003796 0.018227 0.000114 0.000297 0.003369 0.011239 0.000153 0.000074 0.003566 0.012800 0.000127 0.000160
Lipid Particles 0.008493 0.006252 0.000914 0.005013 0.006562 0.016249 0.001674 0.000121 0.007451 0.014016 0.001160 0.001995
Mitochondria 0.003002 0.014051 0.000353 0.000948 0.004828 0.015649 0.000229 0.000499 0.003988 0.015292 0.000313 0.000671
Mitotic Spindle 0.000047 0.001551 0.002658 0.000710 0.001745 0.008831 0.000428 0.002658 0.000964 0.007204 0.001938 0.001912
None 0.002011 0.000934 0.002555 0.010006 0.001784 0.001373 0.003008 0.005043 0.001889 0.001275 0.002701 0.006944
Nuclear Periphery 0.000222 0.000121 0.000383 0.001407 0.000226 0.000292 0.000403 0.000231 0.000224 0.000254 0.000389 0.000682
Nuclear Periphery Foci 0.000224 0.000180 0.001345 0.000780 0.000308 0.000391 0.001611 0.000043 0.000269 0.000344 0.001431 0.000325
Nucleolus 0.000435 0.000759 0.000222 0.002438 0.000743 0.001856 0.000485 0.000163 0.000601 0.001611 0.000307 0.001034
Nucleus 0.064708 0.045856 0.149582* 0.416269* 0.104876 0.086731 0.200184* 0.194397* 0.086394 0.077599 0.165926* 0.279374*
Peroxisomes 0.004193 0.041925 0.000793 0.003034 0.003985 0.020758 0.001511 0.000538 0.004081 0.025487 0.001025 0.001494
Vacuole 0.075920 0.076344 0.107534 0.045698 0.053439 0.065207 0.070360 0.030625 0.063783 0.067695 0.095527 0.036398
Vacuole Periphery 0.001077 0.002299 0.000192 0.001018 0.000937 0.002200 0.000174 0.000061 0.001001 0.002222 0.000186 0.000427

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.97 -1.66 1.43 3.70 2.43 -4.58 -3.69 4.34 5.86 5.31 -4.90 -3.23 3.93 6.36 4.78
Bud Neck -1.69 10.52 10.62 6.24 1.33 -3.37 4.93 3.65 6.19 -1.83 -1.07 10.02 11.13 8.52 1.03
Bud Site -1.00 -5.23 5.34 4.40 9.07 -4.22 -4.39 -1.22 3.39 3.45 -2.24 -8.03 4.27 4.69 10.23
Cell Periphery 5.18 7.87 3.10 -3.01 -6.98 -2.98 -2.27 9.70 4.01 10.54 -1.21 6.40 6.68 3.05 0.89
Cytoplasm 12.90 -8.48 11.35 -6.37 18.35 12.56 -5.18 -12.44 -22.19 -4.79 16.56 -9.34 -3.21 -18.02 5.55
Cytoplasmic Foci -8.44 37.53 29.47 18.37 -8.57 -6.73 29.67 43.77 35.54 14.99 -10.42 49.47 53.58 38.27 6.62
Eisosomes 5.68 14.82 13.48 5.35 -10.42 -5.21 10.62 17.64 7.96 9.13 -1.14 17.17 17.42 8.57 1.40
Endoplasmic Reticulum 0.87 -5.91 -6.01 -6.38 -2.87 0.27 -4.73 3.87 5.91 9.49 0.28 -6.59 -0.90 -1.34 6.61
Endosome -4.86 14.77 10.33 6.59 -4.40 -5.42 8.11 12.28 12.03 10.89 -6.40 16.59 16.64 12.07 2.00
Golgi -3.97 11.98 10.96 4.97 -2.04 -4.41 9.19 9.46 6.45 4.64 -5.77 14.59 14.34 8.05 -0.70
Lipid Particles 2.20 10.00 3.97 1.30 -11.49 -5.15 8.20 12.30 8.95 5.63 -4.41 13.67 11.45 8.41 -4.98
Mitochondria -3.08 3.99 3.08 3.74 -3.80 -3.40 4.18 4.00 5.15 -2.52 -4.46 5.51 5.03 6.05 -3.29
Mitotic Spindle -2.87 -3.29 -3.15 1.72 2.39 -2.93 1.29 -0.13 2.94 -1.80 -3.43 -1.61 -0.83 3.19 0.97
None 2.02 -0.61 -5.79 -6.54 -5.58 1.11 -1.74 -10.21 -8.22 -2.53 1.86 -1.66 -8.31 -8.97 -6.70
Nuclear Periphery 0.51 -2.45 -10.92 -17.25 -15.99 -2.21 -6.98 -6.13 -3.78 2.15 -0.92 -4.86 -10.94 -16.19 -11.87
Nuclear Periphery Foci 0.61 -12.69 -5.90 -5.84 4.44 -1.11 -8.65 5.95 4.97 10.46 -1.46 -14.87 -2.21 -0.35 12.68
Nucleolus -2.08 7.75 -3.77 -2.97 -4.18 -1.47 1.28 2.78 2.29 2.33 -1.75 2.61 -1.78 1.05 -3.58
Nucleus 3.18 -22.00 -38.95 -35.70 -27.30 3.67 -13.98 -24.72 -24.92 -3.63 2.21 -22.80 -43.80 -39.73 -23.18
Peroxisomes -6.54 8.94 1.24 6.68 -2.29 -7.36 2.98 5.07 9.30 2.51 -9.83 7.03 4.91 11.09 -0.70
Vacuole -0.64 -11.45 6.94 3.55 16.74 -3.50 -6.31 -1.82 2.00 4.51 -2.06 -14.88 3.17 3.87 16.29
Vacuole Periphery -3.08 11.77 0.17 1.79 -1.28 -1.66 7.51 8.82 2.89 6.60 -2.05 12.54 2.45 2.87 -0.95
Standard name
Human Ortholog
Description Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency
Localization
Cell Percentages cytoplasm (70%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Zrg8

Zrg8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zrg8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available