ORF
Human Ortholog
Description Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.96 0.96 0.73 0.58 0.53 0.37 – 0.46 0.36 0.32 0.29 0.98 0.98 0.98 0.84 0.86 0.86 0.84 0.82 0.94 0.93 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0 – 0.08 0.06 0.08 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.09 0.12 0.14 – 0.18 0.21 0.25 0.24 0 0 0 0 0 0 0.08 0.1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0 0.09 0.4 0.52 0.36 0.62 – 0.42 0.45 0.45 0.44 0 0 0 0.3 0.22 0.25 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 2 0 0 3 0 0 0 0 0 0 0 1 0 0 2 0
Bud 0 1 0 0 0 2 0 – 1 2 1 7 0 0 0 0 0 0 1 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 0 3 1 3 – 2 3 3 4 1 0 1 3 5 1 0 0 0 0 0 0
Cytoplasm 421 383 340 243 220 237 257 – 230 182 157 163 309 449 489 209 174 160 450 330 328 213 505 415
Endoplasmic Reticulum 1 5 2 0 3 1 1 – 4 2 1 4 5 3 1 5 7 2 0 1 2 0 4 3
Endosome 1 0 0 0 4 0 12 – 3 5 3 9 0 0 0 0 0 0 9 2 0 0 2 2
Golgi 5 1 0 0 3 0 1 – 2 5 8 3 0 0 0 1 0 1 6 2 0 2 0 1
Mitochondria 4 1 1 2 2 45 15 – 38 32 41 65 0 0 0 0 1 0 4 2 1 0 0 1
Nucleus 8 12 9 14 34 53 99 – 88 107 125 133 3 5 4 8 5 4 42 41 8 4 2 6
Nuclear Periphery 1 0 0 0 0 1 0 – 0 0 0 1 0 0 0 0 0 0 1 0 1 0 2 0
Nucleolus 3 0 0 0 0 0 0 – 2 0 1 4 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 62 16 31 133 197 162 431 – 212 231 220 247 2 12 13 74 45 47 12 17 1 4 7 9
Unique Cell Count 475 401 356 334 381 451 697 499 509 491 560 314 457 499 248 202 187 538 404 349 229 532 448
Labelled Cell Count 507 420 385 392 466 503 819 584 569 560 644 320 469 508 300 237 215 538 404 349 229 532 448


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.8 10.2 9.8 9.8 10.6 7.6 10.0 – 9.8 9.9 10.0 9.6 12.1 11.3 12.2 16.2 17.4 16.5 10.0 10.7 11.4
Std Deviation (1e-4) 1.6 1.4 1.2 1.1 1.7 1.8 1.6 – 1.9 1.8 1.9 2.0 1.7 1.6 1.8 3.6 4.0 3.4 1.7 1.6 1.9
Intensity Change (Log2) – – – -0.0 0.11 -0.36 0.03 – -0.01 0.02 0.02 -0.04 0.3 0.2 0.31 0.72 0.83 0.75 0.02 0.13 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -8.3 -12.0 -13.4 -18.2 – -15.1 -17.7 -18.5 -19.7 2.2 2.3 2.1 -4.7 -3.9 -4.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.9 2.3 – 5.1 4.5 5.3 6.5 0 0 0 0 0 0
Nucleus 1.2 3.7 4.9 5.9 – 6.9 7.9 9.0 8.7 -1.5 -1.6 -2.0 0.5 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 9.6 12.6 9.0 16.4 – 10.8 11.6 11.4 11.4 -4.8 -3.8 -4.0 6.7 4.5 5.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7981 6.1419 5.6901 5.3002 4.7357 5.6498 7.5153 8.3518 8.0412 7.2232 7.5776 7.9939 10.0352 11.4327 10.569 10.7263 10.6735 10.8631
Actin 0.0209 0.0002 0.0032 0.0139 0.0027 0.0035 0.0028 0.0005 0.0003 0.0002 0.0001 0.0003 0.0196 0.0003 0.0028 0.0004 0.016 0.0052
Bud 0.0008 0.0001 0.0002 0.0004 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Neck 0.0049 0.0003 0.0008 0.0028 0.0014 0.0017 0.0009 0.0004 0.0005 0.0012 0.0014 0.0017 0.0025 0.0002 0.0007 0.0011 0.0014 0.0013
Bud Periphery 0.0011 0 0.0001 0.0005 0.0001 0.0001 0 0 0.0001 0 0 0 0.0003 0 0.0001 0 0.0002 0.0001
Bud Site 0.0028 0.0008 0.0003 0.0019 0.0003 0.0001 0.0003 0.0005 0.0003 0.0001 0.0001 0.0001 0.0013 0.0002 0.0004 0.0001 0.0005 0.0001
Cell Periphery 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0
Cytoplasm 0.7492 0.8601 0.7965 0.6602 0.7242 0.8067 0.8506 0.8704 0.8393 0.8265 0.8361 0.7891 0.8316 0.9248 0.8493 0.8296 0.8943 0.8563
Cytoplasmic Foci 0.0144 0.0019 0.0023 0.0076 0.0109 0.0045 0.0031 0.0061 0.0032 0.0027 0.004 0.0034 0.0079 0.0014 0.0068 0.0011 0.0041 0.0034
Eisosomes 0.0003 0 0 0.0001 0 0 0 0 0 0 0 0 0.0002 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0072 0.0021 0.0035 0.0039 0.0069 0.0028 0.0037 0.0035 0.0022 0.002 0.0028 0.0029 0.005 0.0011 0.002 0.0029 0.0033 0.0029
Endosome 0.0213 0.0041 0.0042 0.0196 0.0453 0.005 0.0031 0.0032 0.0016 0.0017 0.0028 0.0037 0.0163 0.0005 0.0024 0.0008 0.0094 0.0039
Golgi 0.0051 0.0001 0.0008 0.0077 0.0045 0.0009 0.0003 0.0003 0.0001 0 0 0.0001 0.0044 0 0.0006 0 0.003 0.0009
Lipid Particles 0.0047 0 0.0012 0.0064 0.0096 0.0006 0.0003 0 0 0 0 0.0001 0.0037 0 0.0007 0 0.0045 0.0003
Mitochondria 0.006 0.0002 0.0005 0.0017 0.0029 0.0005 0.0004 0.0003 0.0003 0.0003 0.0002 0.0003 0.0025 0.0003 0.0003 0.0002 0.0014 0.0005
None 0.0081 0.003 0.0032 0.0097 0.0046 0.0027 0.0064 0.0033 0.0077 0.0028 0.0053 0.0041 0.0078 0.0027 0.0107 0.0016 0.0035 0.0057
Nuclear Periphery 0.0154 0.0074 0.0123 0.0089 0.023 0.0086 0.0075 0.0054 0.006 0.0074 0.0069 0.0078 0.0105 0.0028 0.0049 0.007 0.0104 0.0078
Nucleolus 0.0021 0.0001 0.0001 0.0005 0.0009 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0006 0.0001 0.0001 0.0001 0.0004 0.0001
Nucleus 0.1072 0.1145 0.1663 0.2441 0.1362 0.1539 0.1168 0.1026 0.134 0.152 0.1357 0.18 0.076 0.0641 0.112 0.1517 0.0396 0.1085
Peroxisomes 0.0098 0 0.0003 0.0022 0.0019 0.0004 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001 0.0028 0.0001 0.0022 0 0.0011 0.0002
Punctate Nuclear 0.0145 0.0028 0.0027 0.006 0.0202 0.0058 0.0019 0.0021 0.0019 0.0017 0.003 0.0043 0.0048 0.0008 0.0032 0.0027 0.0048 0.0018
Vacuole 0.0027 0.0019 0.0013 0.0012 0.0029 0.0015 0.0012 0.0008 0.0015 0.0008 0.0011 0.0011 0.0013 0.0004 0.0004 0.0004 0.0008 0.0007
Vacuole Periphery 0.0014 0.0004 0.0004 0.0005 0.0012 0.0003 0.0002 0.0001 0.0003 0.0002 0.0002 0.0002 0.0006 0.0001 0.0001 0.0001 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.7178 15.9093 19.6756 20.5191 14.101 17.787 21.4478 30.7612 24.5579 19.3439
Translational Efficiency 1.1362 2.0611 1.933 1.7455 1.9821 1.5924 1.6986 1.5549 1.8267 1.5433

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1807 1237 260 180 1066 1980 1493 905 2873 3217 1753 1085

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 943.00 999.93 1304.10 1418.22 968.40 1065.81 1214.20 1401.96 952.42 1040.48 1227.53 1404.66
Standard Deviation 126.88 218.02 168.60 221.52 134.03 147.76 173.70 220.99 130.16 180.95 175.88 221.16
Intensity Change Log 2 — 0.084569 0.467725 0.588752 — 0.138275 0.326331 0.533770 — 0.112029 0.397820 0.561158

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000223 0.000215 0.000585 0.000739 0.000211 0.000444 0.000299 0.005420 0.000219 0.000356 0.000341 0.004644
Bud Neck 0.001948 0.001267 0.003060 0.002502 0.001042 0.002217 0.001444 0.007769 0.001612 0.001852 0.001683 0.006895
Bud Site 0.002992 0.005284 0.002395 0.005055 0.002107 0.005318 0.001687 0.039313 0.002663 0.005305 0.001792 0.033629
Cell Periphery 0.000108 0.000229 0.000149 0.000257 0.000119 0.000061 0.000080 0.000293 0.000112 0.000125 0.000090 0.000287
Cytoplasm 0.626424* 0.720358* 0.249717* 0.383085* 0.633174* 0.716536* 0.392317* 0.418062* 0.628928* 0.718006* 0.371167* 0.412260*
Cytoplasmic Foci 0.041153 0.014586 0.002081 0.005384 0.029643 0.021668 0.001579 0.038136 0.036882 0.018945 0.001654 0.032703
Eisosomes 0.000019 0.000010 0.000014 0.000022 0.000020 0.000012 0.000006 0.000027 0.000020 0.000011 0.000007 0.000026
Endoplasmic Reticulum 0.003006 0.001138 0.002527 0.002130 0.003380 0.001170 0.001141 0.002569 0.003145 0.001157 0.001347 0.002496
Endosome 0.001655 0.001525 0.000903 0.002130 0.002530 0.002452 0.000272 0.028346 0.001980 0.002095 0.000365 0.023997
Golgi 0.000558 0.000916 0.000120 0.000267 0.000404 0.000677 0.000070 0.026886 0.000501 0.000769 0.000078 0.022470
Lipid Particles 0.000971 0.000368 0.000668 0.001648 0.001180 0.000771 0.000276 0.001539 0.001049 0.000616 0.000334 0.001557
Mitochondria 0.001283 0.000950 0.000693 0.000493 0.000331 0.000688 0.000478 0.009040 0.000930 0.000789 0.000510 0.007622
Mitotic Spindle 0.001354 0.004270 0.006856 0.111535* 0.001647 0.009080 0.003180 0.102859* 0.001462 0.007230 0.003725 0.104298*
None 0.003136 0.025079 0.007779 0.016780 0.006165 0.007412 0.010473 0.007656 0.004260 0.014205 0.010073 0.009170
Nuclear Periphery 0.000461 0.000270 0.001095 0.001074 0.000822 0.000364 0.000636 0.005294 0.000595 0.000328 0.000704 0.004594
Nuclear Periphery Foci 0.002687 0.002570 0.001723 0.007547 0.003418 0.001769 0.001228 0.003248 0.002958 0.002077 0.001302 0.003961
Nucleolus 0.000450 0.000559 0.000183 0.000194 0.000564 0.000327 0.000114 0.001797 0.000493 0.000416 0.000124 0.001531
Nucleus 0.281911* 0.201806* 0.684786* 0.313164* 0.279631* 0.193277* 0.566679* 0.226600* 0.281065* 0.196557* 0.584197* 0.240961*
Peroxisomes 0.000418 0.000279 0.000070 0.000098 0.000211 0.001042 0.000037 0.004764 0.000341 0.000749 0.000042 0.003990
Vacuole 0.028544 0.017538 0.034504 0.145773 0.032430 0.034437 0.017949 0.055563 0.029986 0.027939 0.020405 0.070529
Vacuole Periphery 0.000699 0.000783 0.000092 0.000122 0.000973 0.000279 0.000056 0.014819 0.000801 0.000473 0.000061 0.012381

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.15 -6.84 -6.36 -6.52 0.63 -1.41 -6.05 -3.12 -2.25 -2.42 -1.40 -8.32 -3.43 -2.80 -2.39
Bud Neck 1.68 -5.36 -5.07 -6.25 0.98 -2.71 -6.39 -10.63 -5.99 -7.57 -1.00 -5.44 -10.09 -8.35 -6.85
Bud Site -2.26 -1.75 -4.50 -1.23 -1.80 -4.37 -0.52 -5.54 -3.67 -5.40 -4.33 -0.08 -5.63 -3.84 -5.59
Cell Periphery -1.56 -6.12 -4.68 -0.24 -0.05 5.96 1.17 -3.40 -8.10 -4.71 -0.21 -2.07 -5.57 -2.31 -4.48
Cytoplasm -9.52 30.96 21.49 25.86 -2.55 -7.61 30.31 23.86 34.55 -2.45 -12.22 44.04 32.65 42.36 -2.92
Cytoplasmic Foci 11.94 20.62 19.20 9.25 -3.01 3.38 14.33 9.00 7.79 -5.74 10.83 25.04 16.58 8.51 -5.98
Eisosomes 4.11 -1.66 -4.76 -7.02 -2.46 4.62 6.54 -1.27 -7.25 -10.16 6.40 7.42 -3.63 -9.28 -10.41
Endoplasmic Reticulum 7.55 -0.99 2.94 -4.17 2.93 3.71 3.23 3.61 -0.54 1.55 7.23 5.06 6.39 -1.69 2.58
Endosome 0.54 1.25 -0.16 -0.60 -1.20 0.33 9.21 -1.95 -1.98 -4.74 -0.23 11.14 -2.63 -2.39 -4.84
Golgi -0.78 2.90 2.51 1.81 -1.42 -0.91 2.13 -2.84 -2.63 -3.15 -1.05 4.10 -2.72 -2.47 -3.15
Lipid Particles 4.14 -1.58 -4.26 -6.33 -3.43 1.93 3.78 -2.03 -4.47 -8.91 2.96 3.86 -4.76 -7.53 -9.59
Mitochondria 0.57 0.59 0.98 0.54 2.51 -2.43 -3.00 -2.26 -1.68 -1.84 0.36 0.67 -1.08 -1.52 -1.78
Mitotic Spindle -2.11 -1.66 -2.85 -2.56 -2.18 -4.11 -1.20 -8.06 -6.11 -7.75 -4.80 -2.29 -8.61 -6.99 -7.94
None -7.04 -3.59 -2.47 2.81 -1.49 -0.78 -2.74 -3.78 -3.99 -2.24 -7.22 -8.50 -7.84 1.81 -2.58
Nuclear Periphery 2.16 -11.01 -11.01 -11.36 -2.59 2.34 -1.26 -3.00 -3.79 -2.75 2.97 -7.72 -4.08 -4.55 -2.96
Nuclear Periphery Foci -0.33 0.26 -2.31 -2.13 -2.22 3.79 5.86 3.27 -1.33 -3.85 2.48 5.33 0.88 -1.65 -4.39
Nucleolus -0.64 0.86 -0.01 0.95 -1.49 1.01 1.82 0.06 -1.35 -2.61 0.53 2.45 -0.31 -0.91 -2.79
Nucleus 9.69 -24.57 -2.80 -6.31 13.39 9.92 -33.45 -6.35 -16.37 23.99 14.61 -46.08 -7.75 -17.58 27.25
Peroxisomes 0.83 1.91 1.86 2.03 -0.47 -2.94 4.63 -1.29 0.62 -1.77 -1.99 2.85 -0.84 0.28 -1.77
Vacuole 1.87 -5.51 -13.30 -13.50 -10.38 -2.99 -1.73 -19.33 -18.01 -18.68 -2.89 -5.53 -23.91 -22.82 -21.05
Vacuole Periphery -0.04 2.34 2.33 1.76 -0.14 1.61 2.12 -1.36 -2.55 -2.86 1.39 3.31 -1.53 -2.17 -2.86
ORF
Human Ortholog
Description Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YER034W

YER034W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yer034w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available