Standard name | |
---|---|
Human Ortholog | |
Description | RNA-binding protein that directly activates mRNA decapping; binds mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein increases in abundance and relocalizes to nucleolus and to nuclear foci upon DNA replication stress; EDC2 has a paralog, EDC1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 5 | 6 | 2 | 0 | 2 | 0 | 2 | 2 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 16 | 6 | 0 | 2 | – | 10 | 4 | 1 | 3 | 4 | 1 | 1 | 1 | 0 | 0 | 0 | 6 | 9 | 4 | 4 | 9 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 5 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 5 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 440 | 419 | 249 | 167 | 110 | 103 | – | 43 | 59 | 46 | 39 | 43 | 160 | 253 | 363 | 235 | 123 | 184 | 385 | 331 | 328 | 158 | 198 | 210 |
Endoplasmic Reticulum | 5 | 6 | 1 | 3 | 3 | 0 | – | 0 | 1 | 0 | 0 | 0 | 6 | 6 | 1 | 14 | 7 | 11 | 2 | 2 | 4 | 3 | 4 | 6 |
Endosome | 0 | 0 | 2 | 7 | 14 | 17 | – | 12 | 3 | 7 | 8 | 4 | 20 | 22 | 10 | 3 | 3 | 6 | 14 | 14 | 11 | 10 | 10 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 0 | 0 | 9 | 5 | 1 | 19 | 11 | 24 | 2 | 2 | 1 | 2 | 0 | 0 |
Mitochondria | 7 | 2 | 23 | 22 | 15 | 37 | – | 73 | 119 | 114 | 116 | 133 | 0 | 1 | 0 | 1 | 5 | 1 | 3 | 2 | 3 | 0 | 0 | 1 |
Nucleus | 12 | 18 | 10 | 7 | 93 | 182 | – | 212 | 328 | 333 | 269 | 322 | 2 | 2 | 2 | 2 | 2 | 1 | 67 | 122 | 61 | 26 | 17 | 20 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 7 | 1 | 3 | 4 | 2 |
Nucleolus | 0 | 0 | 0 | 2 | 1 | 14 | – | 118 | 43 | 61 | 42 | 69 | 2 | 1 | 2 | 1 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 7 | 5 | 7 | 4 | 3 | 2 |
Vac/Vac Membrane | 78 | 198 | 156 | 93 | 247 | 250 | – | 212 | 182 | 157 | 94 | 125 | 127 | 75 | 44 | 273 | 144 | 255 | 20 | 49 | 39 | 18 | 25 | 30 |
Unique Cell Count | 499 | 537 | 416 | 269 | 444 | 537 | 608 | 639 | 651 | 500 | 616 | 288 | 351 | 412 | 451 | 241 | 397 | 524 | 561 | 475 | 240 | 285 | 299 | |
Labelled Cell Count | 543 | 644 | 457 | 308 | 483 | 606 | 683 | 742 | 727 | 579 | 708 | 334 | 377 | 429 | 553 | 298 | 486 | 524 | 561 | 475 | 240 | 285 | 299 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 11.1 | 5.9 | 8.0 | 9.7 | 8.8 | – | 9.0 | 8.9 | 9.0 | 9.1 | 9.2 | 13.9 | 14.1 | 12.9 | 18.0 | 17.4 | 17.4 | 11.2 | 12.7 | 13.3 |
Std Deviation (1e-4) | 1.2 | 1.8 | 1.6 | 1.5 | 1.7 | 1.8 | – | 2.0 | 2.3 | 2.3 | 2.6 | 2.4 | 2.6 | 2.6 | 2.8 | 5.5 | 5.4 | 5.3 | 2.5 | 2.5 | 3.1 |
Intensity Change (Log2) | – | – | – | 0.44 | 0.71 | 0.58 | – | 0.6 | 0.59 | 0.6 | 0.62 | 0.64 | 1.23 | 1.25 | 1.13 | 1.6 | 1.56 | 1.56 | 0.92 | 1.1 | 1.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -1.2 | -4.2 | -3.9 | – | -2.2 | -3.7 | -4.7 | -3.4 | -3.7 | -3.0 | -3.3 | -3.7 | -4.2 | -3.1 | -3.9 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -10.4 | -12.9 | – | -18.4 | -17.7 | -18.8 | -16.9 | -18.5 | -1.1 | 3.5 | 9.3 | -2.3 | -2.2 | -3.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 3.0 |
Endosome | 0 | 2.9 | 2.9 | – | 2.0 | 0 | 0 | 0 | 0 | 4.8 | 4.6 | 2.3 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 5.1 |
Mitochondria | 1.4 | -1.5 | 0.9 | – | 3.5 | 6.1 | 5.7 | 7.4 | 7.1 | -4.1 | -4.2 | -4.8 | -4.8 | -2.1 | -4.4 |
Nucleus | 0.2 | 8.4 | 12.0 | – | 12.4 | 16.6 | 16.6 | 16.8 | 16.8 | 0 | -2.0 | -2.3 | -2.5 | 0 | -2.7 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 3.3 | – | 9.6 | 5.4 | 6.4 | 6.1 | 7.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.8 | 5.3 | 2.8 | – | -0.9 | -3.1 | -4.7 | -6.3 | -6.1 | 1.8 | -4.9 | -9.0 | 6.8 | 5.5 | 7.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.7675 | 6.1292 | 5.7431 | 4.7968 | 4.8876 | 5.6965 | 4.0518 | 4.7427 | 4.4874 | 4.2766 | 3.5177 | 4.3789 | 7.7365 | 8.2343 | 7.7236 | 6.925 | 7.1118 | 7.4218 |
Actin | 0.0301 | 0.0016 | 0.0057 | 0.0044 | 0.0002 | 0.0011 | 0.0132 | 0.0014 | 0.011 | 0.0376 | 0.0039 | 0.003 | 0.0046 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0003 |
Bud | 0.0014 | 0.0002 | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0.0016 | 0.0014 | 0.0009 | 0.0004 | 0.0007 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0001 |
Bud Neck | 0.0097 | 0.0006 | 0.0011 | 0.0007 | 0.0008 | 0.0014 | 0.0016 | 0.0004 | 0.0012 | 0.0049 | 0.0023 | 0.0031 | 0.0011 | 0.0008 | 0.0015 | 0.0011 | 0.0019 | 0.0022 |
Bud Periphery | 0.0016 | 0.0001 | 0.0009 | 0.0003 | 0 | 0.0006 | 0.0003 | 0.0001 | 0.0017 | 0.0011 | 0.001 | 0.0003 | 0.0004 | 0.0001 | 0.0005 | 0 | 0 | 0 |
Bud Site | 0.0055 | 0.0046 | 0.0065 | 0.0006 | 0.0001 | 0.0003 | 0.0029 | 0.0043 | 0.0208 | 0.0139 | 0.0016 | 0.0007 | 0.0015 | 0.0023 | 0.0009 | 0.0002 | 0.0002 | 0.0001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.6508 | 0.9113 | 0.8086 | 0.7785 | 0.8456 | 0.8499 | 0.6945 | 0.8406 | 0.8123 | 0.6001 | 0.7271 | 0.7688 | 0.6786 | 0.6966 | 0.7066 | 0.7598 | 0.7027 | 0.672 |
Cytoplasmic Foci | 0.0265 | 0.0023 | 0.0094 | 0.0151 | 0.0089 | 0.0045 | 0.0392 | 0.0084 | 0.0146 | 0.0414 | 0.0137 | 0.0178 | 0.0053 | 0.0049 | 0.0037 | 0.0025 | 0.0046 | 0.0047 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0083 | 0.0028 | 0.004 | 0.0028 | 0.0029 | 0.0047 | 0.0116 | 0.0036 | 0.0065 | 0.0067 | 0.0196 | 0.0057 | 0.0033 | 0.0034 | 0.0029 | 0.0032 | 0.0025 | 0.0035 |
Endosome | 0.0587 | 0.0014 | 0.008 | 0.0294 | 0.0454 | 0.0183 | 0.0384 | 0.0037 | 0.0107 | 0.0451 | 0.0385 | 0.0205 | 0.0114 | 0.0045 | 0.0034 | 0.002 | 0.0025 | 0.0054 |
Golgi | 0.0243 | 0.0001 | 0.0024 | 0.0225 | 0.0103 | 0.0071 | 0.0097 | 0.0003 | 0.0055 | 0.0366 | 0.0068 | 0.0055 | 0.0007 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0002 |
Lipid Particles | 0.018 | 0 | 0.0007 | 0.0022 | 0.0007 | 0.0082 | 0.0105 | 0.0001 | 0.0026 | 0.0082 | 0.0048 | 0.005 | 0.0021 | 0 | 0 | 0 | 0 | 0.0001 |
Mitochondria | 0.0191 | 0.0011 | 0.0192 | 0.0302 | 0.0007 | 0.0073 | 0.0025 | 0.0003 | 0.0033 | 0.0144 | 0.0166 | 0.0033 | 0.0007 | 0.0005 | 0.0006 | 0.0003 | 0.0003 | 0.0004 |
None | 0.0177 | 0.0024 | 0.0046 | 0.002 | 0.0023 | 0.0023 | 0.0265 | 0.0247 | 0.0092 | 0.0125 | 0.0069 | 0.0107 | 0.0171 | 0.0127 | 0.0107 | 0.0085 | 0.0081 | 0.0108 |
Nuclear Periphery | 0.0191 | 0.0056 | 0.0104 | 0.006 | 0.0071 | 0.0098 | 0.0244 | 0.0086 | 0.0087 | 0.0121 | 0.0224 | 0.0146 | 0.0117 | 0.012 | 0.0103 | 0.0099 | 0.008 | 0.0096 |
Nucleolus | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0005 | 0.0009 | 0.0002 | 0.0003 | 0.0085 | 0.0015 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0003 |
Nucleus | 0.0877 | 0.0633 | 0.1063 | 0.0869 | 0.0699 | 0.0663 | 0.0906 | 0.0945 | 0.0742 | 0.117 | 0.1117 | 0.1202 | 0.2522 | 0.2518 | 0.2508 | 0.2062 | 0.2504 | 0.2788 |
Peroxisomes | 0.0105 | 0.0001 | 0.0009 | 0.0125 | 0.0003 | 0.0011 | 0.0132 | 0.0012 | 0.0068 | 0.0124 | 0.002 | 0.0064 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.0031 | 0.0011 | 0.0021 | 0.0017 | 0.0023 | 0.002 | 0.0116 | 0.0052 | 0.0047 | 0.0203 | 0.0089 | 0.0095 | 0.0054 | 0.0058 | 0.0044 | 0.0039 | 0.0164 | 0.0095 |
Vacuole | 0.0046 | 0.0009 | 0.006 | 0.0026 | 0.0018 | 0.0093 | 0.0063 | 0.0016 | 0.0034 | 0.0041 | 0.0076 | 0.0029 | 0.002 | 0.0027 | 0.0021 | 0.0015 | 0.0009 | 0.0014 |
Vacuole Periphery | 0.0021 | 0.0001 | 0.0016 | 0.0009 | 0.0004 | 0.0047 | 0.0016 | 0.0002 | 0.0007 | 0.0015 | 0.0021 | 0.001 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.1535 | 24.772 | 10.0265 | 25.5749 | 26.1299 | 37.7669 | 37.5704 | 44.5677 | 47.3302 | 29.867 |
Translational Efficiency | 1.9947 | 1.8068 | 3.0901 | 1.682 | 2.1022 | 1.9288 | 1.4804 | 2.0759 | 1.7654 | 1.945 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2250 | 1332 | 324 | 1305 | 1088 | 1192 | 221 | 2050 | 3338 | 2524 | 545 | 3355 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 879.71 | 990.44 | 1398.53 | 1337.27 | 796.52 | 967.81 | 1379.98 | 1009.28 | 852.59 | 979.75 | 1391.01 | 1136.86 |
Standard Deviation | 129.73 | 156.30 | 217.88 | 342.17 | 129.02 | 164.33 | 226.48 | 219.73 | 135.24 | 160.54 | 221.59 | 317.19 |
Intensity Change Log 2 | — | 0.171042 | 0.668811 | 0.604191 | — | 0.281013 | 0.792865 | 0.341544 | — | 0.224345 | 0.729091 | 0.485321 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000104 | 0.000300 | 0.000711 | 0.001211 | 0.000694 | 0.000352 | 0.000670 | 0.002585 | 0.000296 | 0.000325 | 0.000694 | 0.002050 |
Bud Neck | 0.002724 | 0.002240 | 0.003597 | 0.014075 | 0.007937 | 0.004096 | 0.009056 | 0.009906 | 0.004423 | 0.003117 | 0.005810 | 0.011528 |
Bud Site | 0.001437 | 0.004642 | 0.002153 | 0.024307 | 0.008574 | 0.008571 | 0.002428 | 0.022686 | 0.003763 | 0.006497 | 0.002264 | 0.023316 |
Cell Periphery | 0.000057 | 0.000095 | 0.000164 | 0.000367 | 0.000295 | 0.000089 | 0.000135 | 0.000152 | 0.000134 | 0.000092 | 0.000152 | 0.000236 |
Cytoplasm | 0.566527* | 0.649878* | 0.182620* | 0.351587* | 0.428136* | 0.628603* | 0.113908* | 0.531801* | 0.521419* | 0.639830* | 0.154757* | 0.461703* |
Cytoplasmic Foci | 0.033206 | 0.037202 | 0.002911 | 0.012817 | 0.028637 | 0.070291 | 0.000362 | 0.009124 | 0.031717 | 0.052829 | 0.001877 | 0.010561 |
Eisosomes | 0.000015 | 0.000020 | 0.000016 | 0.000017 | 0.000032 | 0.000029 | 0.000015 | 0.000015 | 0.000020 | 0.000024 | 0.000016 | 0.000016 |
Endoplasmic Reticulum | 0.002645 | 0.002575 | 0.003773 | 0.004112 | 0.008556 | 0.002819 | 0.004159 | 0.002632 | 0.004572 | 0.002690 | 0.003929 | 0.003208 |
Endosome | 0.001250 | 0.002527 | 0.001425 | 0.007198 | 0.002015 | 0.008781 | 0.000712 | 0.003213 | 0.001499 | 0.005481 | 0.001136 | 0.004763 |
Golgi | 0.000151 | 0.000290 | 0.000182 | 0.008269 | 0.000774 | 0.004214 | 0.000080 | 0.002431 | 0.000354 | 0.002143 | 0.000141 | 0.004702 |
Lipid Particles | 0.000956 | 0.001070 | 0.000871 | 0.001414 | 0.001466 | 0.001376 | 0.000594 | 0.000655 | 0.001122 | 0.001214 | 0.000759 | 0.000950 |
Mitochondria | 0.000966 | 0.000810 | 0.000675 | 0.008146 | 0.001399 | 0.002065 | 0.001379 | 0.003298 | 0.001107 | 0.001403 | 0.000960 | 0.005184 |
Mitotic Spindle | 0.000547 | 0.002420 | 0.000882 | 0.077113 | 0.003315 | 0.015195 | 0.001911 | 0.020924 | 0.001449 | 0.008453 | 0.001299 | 0.042780 |
None | 0.005497 | 0.005577 | 0.004831 | 0.012717 | 0.011553 | 0.003508 | 0.008228 | 0.011427 | 0.007471 | 0.004600 | 0.006209 | 0.011929 |
Nuclear Periphery | 0.000915 | 0.000663 | 0.001268 | 0.003844 | 0.001026 | 0.000678 | 0.002543 | 0.000963 | 0.000951 | 0.000670 | 0.001785 | 0.002083 |
Nuclear Periphery Foci | 0.001900 | 0.002976 | 0.002780 | 0.003326 | 0.003894 | 0.002951 | 0.000607 | 0.001831 | 0.002550 | 0.002964 | 0.001899 | 0.002413 |
Nucleolus | 0.000493 | 0.000393 | 0.000295 | 0.000735 | 0.003092 | 0.001407 | 0.000617 | 0.000576 | 0.001340 | 0.000871 | 0.000425 | 0.000638 |
Nucleus | 0.354870* | 0.249073* | 0.746690* | 0.416100* | 0.460580* | 0.162693* | 0.829921* | 0.339110* | 0.389325* | 0.208279* | 0.780441* | 0.369057* |
Peroxisomes | 0.000380 | 0.000586 | 0.000106 | 0.000583 | 0.000643 | 0.001541 | 0.000076 | 0.002374 | 0.000465 | 0.001037 | 0.000094 | 0.001678 |
Vacuole | 0.025079 | 0.036382 | 0.043902 | 0.046297 | 0.026771 | 0.078100 | 0.022498 | 0.033185 | 0.025630 | 0.056084 | 0.035223 | 0.038285 |
Vacuole Periphery | 0.000281 | 0.000281 | 0.000148 | 0.005764 | 0.000612 | 0.002644 | 0.000102 | 0.001112 | 0.000389 | 0.001397 | 0.000129 | 0.002922 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.87 | -10.23 | -9.53 | -7.03 | -1.00 | 6.14 | -1.37 | -2.65 | -3.39 | -1.63 | -0.31 | -6.12 | -4.62 | -4.55 | -1.89 |
Bud Neck | 1.14 | -4.86 | -13.70 | -14.07 | -11.52 | 3.96 | -3.44 | -3.26 | -6.42 | 1.96 | 2.63 | -5.17 | -13.56 | -14.77 | -4.68 |
Bud Site | -3.58 | -5.27 | -9.93 | -7.16 | -8.28 | 0.46 | 1.93 | -6.45 | -6.09 | -6.59 | -3.19 | -0.94 | -12.53 | -9.40 | -10.77 |
Cell Periphery | -6.42 | -9.42 | -12.62 | -10.63 | -2.30 | 11.23 | 3.39 | 7.23 | -7.76 | 1.81 | 5.78 | -6.11 | -8.05 | -13.00 | 0.59 |
Cytoplasm | -8.40 | 37.82 | 39.75 | 43.72 | -6.79 | -15.71 | 29.28 | -4.59 | 13.85 | -41.85* | -14.95 | 48.55 | 20.28 | 34.49 | -31.03 |
Cytoplasmic Foci | -1.55 | 17.61 | 14.47 | 13.41 | -3.15 | -9.93 | 13.71 | 9.34 | 17.75 | -12.61 | -8.54 | 22.54 | 17.48 | 22.00 | -8.87 |
Eisosomes | -4.21 | -5.95 | -9.85 | -4.08 | 0.78 | 2.02 | 2.16 | 7.07 | 4.99 | 2.71 | -2.77 | -3.73 | -1.00 | 1.92 | 3.16 |
Endoplasmic Reticulum | -0.02 | -5.15 | -2.80 | -4.39 | 3.58 | 9.44 | 3.40 | 9.76 | -1.35 | 3.02 | 5.23 | -2.09 | 3.10 | -3.26 | 4.95 |
Endosome | -4.56 | -4.03 | -3.50 | -0.95 | -0.61 | -6.65 | 4.43 | -0.79 | 5.90 | -3.46 | -8.04 | -2.04 | -3.72 | 4.60 | -1.84 |
Golgi | -2.51 | -4.12 | -2.89 | -2.72 | -2.71 | -3.19 | 1.63 | -1.47 | 2.12 | -3.10 | -3.70 | 0.86 | -3.66 | -0.15 | -4.04 |
Lipid Particles | -0.96 | -3.58 | -7.54 | -7.01 | -2.86 | 1.04 | 0.73 | 2.98 | 1.87 | 0.47 | -0.58 | -2.13 | -4.69 | -4.21 | -0.56 |
Mitochondria | 0.31 | 0.31 | -4.92 | -5.12 | -5.95 | -1.02 | -3.03 | -2.96 | -1.50 | -1.51 | -0.50 | -0.91 | -5.31 | -4.73 | -5.85 |
Mitotic Spindle | -1.95 | -2.45 | -12.18 | -11.53 | -11.90 | -4.37 | -1.93 | -7.08 | -1.63 | -1.19 | -5.19 | -2.31 | -14.42 | -10.04 | -8.97 |
None | 0.04 | 3.26 | -6.48 | -6.41 | -9.71 | 5.03 | 1.65 | 0.98 | -9.65 | -1.26 | 4.41 | 2.55 | -3.49 | -10.74 | -7.18 |
Nuclear Periphery | 2.76 | -7.30 | -10.98 | -11.88 | -6.58 | 3.34 | -8.83 | -4.06 | -6.99 | 7.62 | 3.80 | -11.21 | -11.51 | -13.69 | 1.07 |
Nuclear Periphery Foci | -2.66 | -3.38 | -2.76 | -0.18 | 2.03 | 2.68 | 6.49 | 3.98 | 1.53 | -4.36 | -0.56 | -1.10 | 0.63 | 1.27 | 1.47 |
Nucleolus | 1.53 | 0.67 | -5.83 | -7.72 | -6.93 | 2.83 | 2.39 | 4.17 | 1.46 | 2.45 | 2.41 | 1.99 | 0.67 | -2.56 | -2.03 |
Nucleus | 12.36 | -23.17 | -14.15 | -23.42 | 12.81 | 27.56 | -20.75 | 8.06 | -25.87 | 29.23 | 27.66 | -31.40 | -4.59 | -32.19 | 28.46 |
Peroxisomes | -0.83 | 2.24 | -0.18 | 0.75 | -3.00 | -3.79 | 8.03 | -2.03 | -0.46 | -3.10 | -3.37 | 4.62 | -2.25 | -0.66 | -3.36 |
Vacuole | -5.30 | -7.98 | -17.68 | -14.04 | -1.99 | -12.88 | -2.48 | -8.69 | 6.30 | -1.40 | -14.03 | -8.13 | -19.75 | -5.26 | -0.80 |
Vacuole Periphery | 0.03 | 0.49 | -2.71 | -2.74 | -2.82 | -3.06 | 7.29 | -0.74 | 2.49 | -2.51 | -3.14 | 2.85 | -2.95 | 0.12 | -3.69 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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