Standard name
Human Ortholog
Description Methionine-S-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan; reduced activity of human homolog implicated in Alzheimer disease
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 – 0.86 0.9 0.75 0.78 – 0.75 0.61 – 0.64 1.0 0.99 1.0 0.96 0.97 0.93 0.95 0.96 0.93 0.96 0.96 0.95
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.17 0 – 0.14 0.25 – 0.4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0.14 0 0 0 – 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0.09 0.15 0.17 0.23 – 0.12 0.23 – 0.16 0 0 0 0.07 0.05 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud 0 0 – 0 0 1 1 – 0 0 – 0 0 0 0 0 0 0 1 2 0 0 4 4
Bud Neck 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 – 1 1 0 0 – 1 2 – 1 0 2 1 3 8 5 0 0 0 0 0 0
Cytoplasm 392 612 – 30 93 77 145 – 116 75 – 16 391 818 720 293 300 272 376 600 37 516 656 572
Endoplasmic Reticulum 2 4 – 0 0 0 0 – 2 1 – 0 1 4 2 3 9 4 1 1 0 1 3 1
Endosome 1 0 – 0 0 0 0 – 0 0 – 0 0 1 0 1 0 0 3 1 0 3 2 3
Golgi 3 1 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 6 2 0 0 1 0
Mitochondria 1 0 – 0 2 17 5 – 22 31 – 10 0 0 0 1 2 1 0 1 0 1 2 1
Nucleus 1 8 – 5 1 3 3 – 4 6 – 0 0 1 3 1 6 4 0 4 0 2 1 2
Nuclear Periphery 0 0 – 1 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 – 1 0 0 0 – 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 1 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 1 3 1
Vac/Vac Membrane 4 2 – 3 15 17 42 – 19 28 – 4 0 4 3 22 16 23 1 6 0 4 1 2
Unique Cell Count 400 619 35 103 103 186 154 123 25 392 825 723 304 310 293 396 626 40 536 683 600
Labelled Cell Count 404 629 42 112 115 196 164 144 31 392 830 729 324 341 309 396 626 40 536 683 600


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 8.0 – 11.6 9.6 6.3 9.3 – 7.7 7.0 – 7.3 8.4 8.6 8.6 23.0 22.9 20.4 6.8 7.0 7.2
Std Deviation (1e-4) 1.5 1.4 – 20.5 1.3 1.5 5.3 – 1.9 1.6 – 1.7 1.5 1.6 1.5 5.5 5.7 5.0 1.1 1.3 1.6
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3454 5.4505 5.0486 4.3345 4.8453 4.6614 6.5694 7.8872 7.6556 7.0068 6.4431 7.3554 8.5401 10.6475 9.3152 9.2543 8.0328 9.7738
Actin 0.0041 0.0001 0.0007 0.0012 0.0005 0.0003 0.0109 0.0027 0.0058 0.0004 0.0176 0.0012 0.0233 0.0193 0.0051 0.0523 0.0023 0.0042
Bud 0.0004 0.0001 0.0003 0.0092 0.0025 0.0001 0.0012 0.0053 0.0013 0.0003 0.0004 0.0015 0.0003 0.0001 0.0004 0.0012 0.0002 0.0002
Bud Neck 0.0013 0.0002 0.0009 0.0015 0.0008 0.0062 0.0012 0.0003 0.0006 0.0008 0.0009 0.0014 0.0009 0.0002 0.0012 0.0009 0.0004 0.0009
Bud Periphery 0.0002 0 0.0001 0.0059 0.0013 0 0.0008 0.0034 0.0012 0.0001 0.0003 0.0008 0.0003 0.0001 0.0003 0.0011 0.0003 0.0001
Bud Site 0.0011 0.0005 0.0003 0.0008 0.0008 0.0001 0.0024 0.0025 0.0003 0.0006 0.0007 0.0003 0.001 0.0004 0.001 0.002 0.0002 0.0001
Cell Periphery 0.0001 0 0 0.0001 0.0001 0 0.0001 0.0001 0 0 0 0 0.0001 0 0.0004 0.0001 0.0001 0
Cytoplasm 0.9067 0.9629 0.9477 0.9342 0.939 0.9251 0.861 0.9275 0.9358 0.8917 0.8591 0.8701 0.9031 0.9582 0.933 0.8429 0.9096 0.9425
Cytoplasmic Foci 0.0088 0.0025 0.0035 0.0076 0.0047 0.005 0.0117 0.0052 0.0044 0.0061 0.0165 0.0111 0.008 0.0024 0.0102 0.0144 0.0026 0.0045
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0039 0.0014 0.0033 0.0023 0.0027 0.0029 0.0049 0.0014 0.0016 0.0013 0.003 0.0017 0.0031 0.0014 0.0029 0.0014 0.0066 0.002
Endosome 0.012 0.001 0.0024 0.0026 0.0023 0.0054 0.0173 0.0023 0.0021 0.0028 0.0172 0.0066 0.0063 0.0008 0.002 0.0106 0.0102 0.0042
Golgi 0.0024 0.0001 0.0001 0.0002 0.0001 0.0001 0.0034 0.0004 0.0003 0.0031 0.0043 0.0031 0.0016 0.0005 0.0012 0.0037 0.0008 0.0017
Lipid Particles 0.0009 0 0 0 0.0001 0 0.0015 0.0008 0.0001 0.0002 0.0057 0.0001 0.0016 0.0001 0.0008 0.0002 0.0004 0.0003
Mitochondria 0.0017 0.0001 0.0002 0.0006 0.0002 0.0002 0.0188 0.0156 0.0147 0.0472 0.006 0.0465 0.002 0.0002 0.0009 0.0247 0.003 0.0014
None 0.0276 0.0091 0.0194 0.0124 0.0047 0.017 0.0212 0.0055 0.0089 0.0093 0.0277 0.0156 0.0139 0.0058 0.0145 0.0065 0.0024 0.0052
Nuclear Periphery 0.0053 0.0013 0.0028 0.0021 0.0026 0.0032 0.0075 0.0019 0.002 0.0019 0.0042 0.0026 0.0084 0.0011 0.0012 0.0015 0.03 0.0043
Nucleolus 0.0001 0.0001 0 0.0001 0.0001 0 0.0002 0.0001 0 0 0.0004 0.0001 0.0002 0 0 0.0002 0.0002 0.0001
Nucleus 0.0187 0.0188 0.0157 0.0133 0.0344 0.0312 0.029 0.0173 0.0175 0.0302 0.0181 0.031 0.0126 0.0078 0.0137 0.0241 0.0251 0.025
Peroxisomes 0.0005 0 0.0001 0.0003 0.0001 0.0001 0.002 0.0058 0.0005 0.0002 0.0061 0.0023 0.0021 0.0002 0.0093 0.0083 0.0001 0.0006
Punctate Nuclear 0.0025 0.0009 0.0017 0.0038 0.0017 0.0018 0.0023 0.0009 0.0022 0.0027 0.0097 0.0032 0.0098 0.0009 0.0012 0.0022 0.001 0.002
Vacuole 0.0013 0.0007 0.0008 0.0014 0.0014 0.0011 0.0018 0.0009 0.0005 0.0006 0.0014 0.0007 0.0007 0.0003 0.0005 0.0014 0.002 0.0006
Vacuole Periphery 0.0003 0.0001 0.0001 0.0003 0.0002 0.0002 0.0007 0.0001 0.0001 0.0004 0.0005 0.0002 0.0006 0 0.0001 0.0005 0.0024 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 81.5128 49.7631 39.564 79.037 37.3764 41.7273 48.9677 46.6944 50.6474 49.9952
Translational Efficiency 2.5516 2.8322 3.6028 1.909 2.874 4.4093 2.1512 2.1959 2.6769 2.2224

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1856 105 879 164 202 665 2443 1 2058 770 3322 165

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 702.91 887.83 1051.20 939.10 761.08 1002.67 977.27 370.66 708.62 987.01 996.83 935.65
Standard Deviation 69.56 134.87 128.90 118.45 141.33 169.16 128.12 — 81.38 169.54 132.41 126.06
Intensity Change Log 2 — 0.336943 0.580625 0.417939 — 0.397727 0.360709 -1.037952 — 0.368862 0.470486 -0.160603

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000081 0.001174 0.000264 0.000141 0.000207 0.000545 0.000126 0.000208 0.000093 0.000631 0.000163 0.000141
Bud Neck 0.003076 0.018680 0.001703 0.001714 0.005549 0.002279 0.004654 0.000007 0.003319 0.004516 0.003873 0.001703
Bud Site 0.002476 0.058061 0.003098 0.005078 0.006293 0.012609 0.006042 0.000023 0.002850 0.018807 0.005263 0.005047
Cell Periphery 0.000187 0.000154 0.000087 0.000074 0.000146 0.000230 0.000044 0.000201 0.000183 0.000220 0.000056 0.000075
Cytoplasm 0.622830* 0.535442* 0.538191* 0.544172* 0.597353* 0.690710* 0.732825* 0.000050 0.620329* 0.669537* 0.681325* 0.540875*
Cytoplasmic Foci 0.068231 0.186344* 0.004960 0.043398 0.148519 0.085243 0.007260 0.000015 0.076111 0.099030 0.006651 0.043135
Eisosomes 0.000077 0.000060 0.000017 0.000044 0.000072 0.000041 0.000010 0.000742 0.000077 0.000043 0.000012 0.000048
Endoplasmic Reticulum 0.000571 0.000986 0.002713 0.000844 0.001438 0.002934 0.000554 0.000001 0.000656 0.002668 0.001125 0.000839
Endosome 0.004935 0.010697 0.001079 0.004226 0.010099 0.005940 0.000904 0.000000 0.005442 0.006589 0.000950 0.004200
Golgi 0.000880 0.005067 0.000080 0.000085 0.000703 0.003147 0.000067 0.000010 0.000863 0.003409 0.000071 0.000084
Lipid Particles 0.003884 0.003548 0.000647 0.000914 0.005570 0.003809 0.000362 0.000086 0.004050 0.003774 0.000438 0.000909
Mitochondria 0.002502 0.000232 0.000328 0.000142 0.005401 0.001731 0.000298 0.000024 0.002786 0.001527 0.000306 0.000142
Mitotic Spindle 0.000635 0.003790 0.001993 0.001049 0.012394 0.009704 0.007914 0.000285 0.001789 0.008898 0.006347 0.001045
None 0.083995 0.001357 0.006087 0.002999 0.002053 0.003122 0.007366 0.997642* 0.075952 0.002881 0.007028 0.009027
Nuclear Periphery 0.000295 0.000114 0.001028 0.000505 0.000351 0.000391 0.000522 0.000000 0.000300 0.000354 0.000656 0.000502
Nuclear Periphery Foci 0.007430 0.003338 0.002072 0.001480 0.001983 0.004199 0.000772 0.000002 0.006896 0.004082 0.001116 0.001471
Nucleolus 0.007440 0.000356 0.000148 0.000228 0.000383 0.000312 0.000149 0.000003 0.006747 0.000318 0.000149 0.000227
Nucleus 0.140481* 0.029223 0.389059* 0.094937 0.113571 0.088267 0.186171* 0.000011 0.137840* 0.080215 0.239855* 0.094362
Peroxisomes 0.001288 0.013697 0.000077 0.000312 0.001782 0.000981 0.000265 0.000691 0.001337 0.002715 0.000215 0.000314
Vacuole 0.048407 0.127307 0.046278 0.297434* 0.084904 0.082132 0.043608 0.000000 0.051990 0.088292 0.044315 0.295631*
Vacuole Periphery 0.000301 0.000372 0.000091 0.000224 0.001227 0.001673 0.000086 0.000000 0.000392 0.001495 0.000088 0.000223

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.22 -7.98 -3.81 2.78 3.97 NaN NaN NaN NaN NaN -8.72 -6.42 -3.37 6.38 0.27
Bud Neck -3.05 1.35 3.40 3.45 1.34 NaN NaN NaN NaN NaN -0.77 0.54 3.87 3.57 2.96
Bud Site -4.78 -1.57 -2.15 4.28 -1.08 NaN NaN NaN NaN NaN -7.90 -2.13 -1.93 4.20 -1.05
Cell Periphery 2.10 8.84 15.75 2.12 4.66 NaN NaN NaN NaN NaN -0.90 20.22 15.49 6.95 -0.13
Cytoplasm 3.93 16.83 9.26 3.26 2.62 NaN NaN NaN NaN NaN -1.00 5.72 9.27 9.16 7.81
Cytoplasmic Foci -6.46 25.26 8.80 8.81 -5.33 NaN NaN NaN NaN NaN -3.66 26.97 10.37 11.49 -4.79
Eisosomes 3.06 11.88 5.85 0.67 -5.46 NaN NaN NaN NaN NaN 7.56 15.57 3.99 -1.29 -5.91
Endoplasmic Reticulum -1.03 -9.46 -5.11 -1.47 6.59 NaN NaN NaN NaN NaN -7.21 -8.56 -4.35 4.42 1.57
Endosome -1.64 13.76 4.83 2.64 -3.77 NaN NaN NaN NaN NaN -0.46 16.66 5.70 4.09 -5.14
Golgi -1.14 4.46 4.55 1.46 1.94 NaN NaN NaN NaN NaN -2.08 4.92 4.87 2.98 -1.03
Lipid Particles 0.53 11.07 10.85 0.91 -0.57 NaN NaN NaN NaN NaN 0.65 12.78 10.91 2.26 -3.71
Mitochondria 2.92 2.64 3.00 1.02 7.88 NaN NaN NaN NaN NaN 1.29 2.99 3.30 1.53 8.88
Mitotic Spindle -1.42 -2.96 -2.20 0.31 0.14 NaN NaN NaN NaN NaN -3.18 -5.43 -1.16 2.34 3.16
None 22.82* 21.70 22.51 -1.71 5.57 NaN NaN NaN NaN NaN 22.03* 20.89 9.66 -0.95 -0.21
Nuclear Periphery 4.56 -22.11 -7.55 -9.16 7.40 NaN NaN NaN NaN NaN -1.92 -29.01 -7.44 -4.58 3.94
Nuclear Periphery Foci 6.26 12.77 14.05 1.30 2.18 NaN NaN NaN NaN NaN 6.66 17.54 13.57 4.77 -2.33
Nucleolus 17.51* 18.17 18.17 1.67 0.02 NaN NaN NaN NaN NaN 17.63* 18.11 18.04 2.19 -0.23
Nucleus 20.52 -32.14 3.40 -6.27 23.83 NaN NaN NaN NaN NaN 9.94 -31.61 3.22 -1.48 16.58
Peroxisomes -2.91 7.36 6.54 3.30 -3.14 NaN NaN NaN NaN NaN -1.52 7.71 7.12 4.26 -1.95
Vacuole -6.40 -7.99 -15.40 -8.18 -13.61 NaN NaN NaN NaN NaN -11.13 -13.12 -15.11 -11.74 -13.46
Vacuole Periphery -0.20 6.75 3.57 2.45 -0.94 NaN NaN NaN NaN NaN -1.60 5.31 3.62 2.03 -1.95
Standard name
Human Ortholog
Description Methionine-S-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan; reduced activity of human homolog implicated in Alzheimer disease
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mxr1

Mxr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mxr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available