Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.07 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.96 0.92 0.95 0.88 0.81 0.79 0.75 0.74 0.79 0.63 0.98 0.99 0.97 0.98 0.99 0.97 0.97 0.95 0.94 0.88 0.92 0.92
Endoplasmic Reticulum 0 0 0.06 0.07 0.05 0.1 0.13 0.13 0.17 0.16 0.12 0.16 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 2 4 9 9 5 6 7 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 2 1 0 10 7 8 5 10 6 4 0 4 7 0 3 2 0 0 0 0 0 0
Cytoplasm 129 442 81 82 86 126 129 154 129 118 108 64 116 240 212 241 235 180 121 422 94 74 209 206
Endoplasmic Reticulum 1 11 5 6 5 14 21 25 29 26 17 16 3 3 5 12 15 11 1 6 3 1 6 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 2 1 4 7 10 5 6 7 4 0 0 1 2 1 4 0 0 0 0 0 0
Mitochondria 0 0 0 1 0 2 2 0 11 3 5 8 0 0 1 4 0 1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 1 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 3 6 9 5 4 2 7 1 1 0 2 3 1 0 9 0 3 3 2
Unique Cell Count 130 447 84 89 91 143 160 196 171 159 137 101 118 243 218 246 238 185 126 447 101 85 228 225
Labelled Cell Count 130 456 88 92 96 162 177 215 193 172 151 114 121 248 226 262 257 199 126 447 101 85 228 225


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 118.0 85.2 60.6 53.1 37.1 35.6 29.1 28.3 25.3 26.9 24.8 22.5 52.8 50.3 46.6 83.6 101.8 95.7 57.3 59.8 59.1
Std Deviation (1e-4) 23.1 18.6 21.7 20.8 13.5 14.5 12.0 11.1 9.6 9.5 8.3 9.0 16.8 19.3 18.6 30.1 35.6 39.8 22.8 23.7 22.5
Intensity Change (Log2) – – – -0.19 -0.71 -0.77 -1.06 -1.1 -1.26 -1.17 -1.29 -1.43 -0.2 -0.27 -0.38 0.46 0.75 0.66 -0.08 -0.02 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.2 -0.6 -2.1 -3.4 -3.7 -4.1 -4.3 -3.6 -5.4 0.8 1.4 0.4 0.8 1.3 0.4
Endoplasmic Reticulum 0.2 0 1.0 1.7 1.7 2.4 2.3 1.6 2.1 0 0 0 0 0.1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 80.4418 104.1793 96.3464 94.7704 99.853 95.4871 104.5026 133.0912 122.2913 102.3742 109.2013 115.0115 93.474 111.9561 104.38 105.07 97.4032 101.8531
Actin 0.004 0.0009 0.0114 0.0004 0.001 0.0017 0.0027 0.0014 0.0042 0.0036 0.0012 0.0025 0.0101 0.0006 0.0043 0.0004 0.0005 0.0018
Bud 0.0016 0.0007 0.0025 0.0019 0.0041 0.0024 0.0029 0.0014 0.0027 0.0026 0.0067 0.0039 0.0028 0.0007 0.0028 0.0011 0.0026 0.0008
Bud Neck 0.0011 0.001 0.0014 0.001 0.0012 0.0014 0.0009 0.0007 0.0014 0.0028 0.0022 0.0036 0.0013 0.0008 0.0018 0.0007 0.0014 0.0018
Bud Periphery 0.0042 0.0006 0.0019 0.0015 0.0017 0.0036 0.0048 0.0006 0.0039 0.0026 0.026 0.0047 0.0039 0.0004 0.0029 0.0008 0.0022 0.0004
Bud Site 0.0026 0.0005 0.0029 0.0002 0.0004 0.0004 0.0012 0.0005 0.002 0.007 0.0009 0.0005 0.0017 0.0005 0.0058 0.0002 0.0003 0.0002
Cell Periphery 0.0336 0.0103 0.0282 0.0292 0.0033 0.0101 0.013 0.0083 0.0158 0.0082 0.0041 0.0059 0.021 0.011 0.0194 0.0244 0.0025 0.0029
Cytoplasm 0.5417 0.8754 0.705 0.5319 0.6253 0.6752 0.7337 0.9128 0.7929 0.5842 0.7589 0.7463 0.6428 0.8919 0.7746 0.6456 0.7797 0.7725
Cytoplasmic Foci 0.0144 0.0018 0.0068 0.0027 0.0082 0.004 0.011 0.002 0.005 0.005 0.0096 0.0056 0.0031 0.0011 0.0051 0.0024 0.0234 0.0153
Eisosomes 0.0011 0 0.0009 0.0001 0 0 0.0008 0 0.0002 0 0.0002 0 0.0005 0.0004 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0964 0.0472 0.0238 0.0099 0.012 0.1396 0.0536 0.017 0.0254 0.0262 0.0105 0.0392 0.1114 0.0277 0.0509 0.0098 0.0134 0.0956
Endosome 0.0211 0.0045 0.0146 0.0193 0.038 0.0243 0.0127 0.0045 0.011 0.0603 0.0303 0.0345 0.0143 0.0026 0.017 0.0177 0.0194 0.0324
Golgi 0.0078 0.0019 0.0023 0.0012 0.0021 0.0085 0.0026 0.0011 0.0034 0.009 0.0027 0.0072 0.0028 0.0008 0.0054 0.0007 0.0011 0.0071
Lipid Particles 0.0084 0.0006 0.004 0.0016 0.002 0.0008 0.003 0.0005 0.0015 0.0018 0.0015 0.0003 0.0019 0.0005 0.002 0.0017 0.0025 0.0009
Mitochondria 0.0165 0.0007 0.0052 0.0015 0.0007 0.0066 0.0057 0.0008 0.0051 0.0061 0.0135 0.006 0.0107 0.0005 0.0061 0.0004 0.0136 0.0016
None 0.0057 0.0002 0.0053 0.0002 0.0003 0.0009 0.0051 0.0005 0.0045 0.0012 0.0095 0.0006 0.0083 0.0003 0.0026 0.0003 0.0127 0.0009
Nuclear Periphery 0.0457 0.0036 0.011 0.0091 0.0151 0.0104 0.0236 0.0024 0.0067 0.0163 0.007 0.0186 0.0313 0.0119 0.0101 0.0093 0.0043 0.0109
Nucleolus 0.0022 0.0001 0.0086 0.0002 0.0002 0.009 0.0023 0.0002 0.0015 0.0005 0.0045 0.0003 0.0006 0.0001 0.0014 0.0007 0.0018 0.0002
Nucleus 0.0091 0.0016 0.0062 0.0035 0.0042 0.0117 0.0064 0.0018 0.0034 0.0074 0.0052 0.0401 0.0072 0.0024 0.0041 0.0032 0.0037 0.0041
Peroxisomes 0.0065 0 0.0025 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0003 0.0006 0.0001 0.0003 0 0.0006 0 0.0007 0.0005
Punctate Nuclear 0.001 0 0.0009 0 0.0001 0.0001 0.0032 0.0001 0.0002 0.0006 0.0004 0.0002 0.0005 0 0.0004 0 0.0004 0.0081
Vacuole 0.1344 0.0428 0.1243 0.2672 0.2023 0.0608 0.0895 0.0399 0.0869 0.2047 0.0739 0.056 0.0971 0.0416 0.0697 0.2193 0.0791 0.0342
Vacuole Periphery 0.0407 0.0055 0.0303 0.1173 0.0778 0.0284 0.0209 0.0035 0.0224 0.0495 0.0307 0.024 0.0266 0.0041 0.0129 0.0611 0.0346 0.0079

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1227.0099 1153.4672 1343.8782 1452.8401 1606.507 1144.977 1145.8325 1161.419 1287.8639 1232.0724
Translational Efficiency 1.9107 2.141 1.8894 1.6414 1.7999 2.0512 1.8572 1.9939 1.6933 1.7465

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
496 829 1877 131 2044 1972 191 1560 2540 2801 2068 1691

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3984.27 4579.34 5228.55 4453.19 3966.13 4085.23 5296.58 4695.52 3969.67 4231.47 5234.83 4676.75
Standard Deviation 1121.52 1473.12 1339.46 1059.75 1143.17 1209.30 1442.19 1029.17 1138.99 1312.53 1349.42 1033.60
Intensity Change Log 2 — 0.200824 0.392095 0.160524 — 0.042685 0.417329 0.243553 — 0.124101 0.404739 0.202541

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000185 0.000136 0.000110 0.000290 0.000048 0.000083 0.000243 0.000155 0.000075 0.000099 0.000123 0.000165
Bud Neck 0.000676 0.000787 0.001257 0.003627 0.000254 0.000723 0.007943 0.000798 0.000336 0.000742 0.001874 0.001018
Bud Site 0.011820 0.007403 0.003351 0.011136 0.003518 0.008307 0.004691 0.005290 0.005139 0.008040 0.003475 0.005743
Cell Periphery 0.057224 0.031315 0.014894 0.080022 0.010449 0.003156 0.027137 0.025046 0.019583 0.011490 0.016024 0.029305
Cytoplasm 0.225720* 0.508934* 0.667960* 0.301851* 0.657866* 0.788063* 0.292344* 0.701287* 0.573479* 0.705450* 0.633268* 0.670343*
Cytoplasmic Foci 0.028609 0.013206 0.006404 0.012787 0.016835 0.014971 0.022490 0.005205 0.019134 0.014449 0.007889 0.005793
Eisosomes 0.000005 0.000003 0.000002 0.000011 0.000001 0.000001 0.000009 0.000003 0.000002 0.000002 0.000003 0.000003
Endoplasmic Reticulum 0.006591 0.002320 0.000846 0.000527 0.004250 0.001665 0.000392 0.000838 0.004707 0.001859 0.000804 0.000814
Endosome 0.003833 0.001864 0.000699 0.001476 0.001371 0.001941 0.001916 0.000711 0.001852 0.001919 0.000812 0.000770
Golgi 0.246963* 0.087486 0.040965 0.058758 0.069356 0.028762 0.077245 0.028658 0.104038 0.046142 0.044316 0.030990
Lipid Particles 0.037327 0.028114 0.010809 0.048242 0.009206 0.003638 0.027719 0.018289 0.014697 0.010882 0.012371 0.020609
Mitochondria 0.000200 0.000356 0.000240 0.000079 0.000365 0.000689 0.000148 0.000185 0.000333 0.000590 0.000232 0.000176
Mitotic Spindle 0.002677 0.001516 0.001394 0.001264 0.001273 0.002222 0.004668 0.000425 0.001547 0.002013 0.001697 0.000490
None 0.000951 0.003365 0.001363 0.000792 0.001849 0.003135 0.001199 0.001236 0.001674 0.003203 0.001348 0.001201
Nuclear Periphery 0.000858 0.000856 0.001303 0.001319 0.000553 0.000267 0.003200 0.000437 0.000612 0.000442 0.001478 0.000505
Nuclear Periphery Foci 0.013298 0.018081 0.015354 0.016689 0.013455 0.006019 0.032308 0.008827 0.013425 0.009589 0.016920 0.009436
Nucleolus 0.000080 0.000139 0.000090 0.000114 0.000061 0.000142 0.000175 0.000092 0.000065 0.000141 0.000098 0.000093
Nucleus 0.001471 0.007464 0.010884 0.011459 0.002815 0.010363 0.018776 0.007450 0.002553 0.009505 0.011613 0.007761
Peroxisomes 0.000064 0.000066 0.000022 0.000096 0.000045 0.000134 0.000021 0.000065 0.000049 0.000114 0.000022 0.000068
Vacuole 0.118838 0.161439 0.141081 0.384671* 0.066821 0.064795 0.385068* 0.160303 0.076979 0.093398 0.163615 0.177685
Vacuole Periphery 0.242608* 0.125149* 0.080970 0.064791 0.139608* 0.060923 0.092308 0.034702 0.159722* 0.079931 0.082017 0.037033

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.29 1.59 -2.35 -3.42 -3.62 -2.27 -6.79 -4.08 -2.59 2.02 -2.03 -4.23 -3.77 -2.65 -1.19
Bud Neck -1.15 -4.86 -3.97 -3.78 -2.72 -6.76 -7.65 -8.75 -1.17 7.10 -7.09 -10.26 -8.37 -3.10 5.70
Bud Site 2.00 4.11 0.23 -1.16 -2.57 -6.62 -2.53 -3.28 3.10 -0.56 -4.52 1.81 -1.37 2.72 -2.97
Cell Periphery 5.94 10.61 -2.19 -5.49 -7.64 10.03 -5.23 -9.28 -14.95 0.42 6.81 2.88 -5.20 -10.71 -7.69
Cytoplasm -15.10 -27.78 -2.67 6.50 12.05 -12.77 15.58 -3.30 8.80 -16.99 -13.14 -4.38 -8.25 3.72 -3.80
Cytoplasmic Foci 5.52 8.28 3.89 -0.35 -2.78 1.39 -3.23 10.80 11.47 6.90 4.04 9.45 12.67 11.63 4.46
Eisosomes 3.59 3.67 -3.69 -5.34 -5.41 -0.66 -7.11 -3.90 -3.40 5.39 0.64 -4.10 -3.59 -3.84 -0.16
Endoplasmic Reticulum 4.26 5.98 6.32 5.80 3.39 5.16 8.30 7.10 3.58 -3.23 6.37 9.27 8.97 5.19 -0.01
Endosome 4.70 12.24 7.12 1.16 -3.28 -3.46 -2.72 6.38 7.18 5.79 -0.64 10.81 10.28 7.29 0.93
Golgi 16.99 24.07 19.35 4.70 -2.90 14.34 -0.28 14.96 0.05 7.99 17.33 19.04 23.13 6.96 6.95
Lipid Particles 2.37 7.96 -1.84 -3.92 -8.17 6.20 -7.72 -7.16 -12.90 3.84 3.25 1.78 -4.37 -7.64 -6.53
Mitochondria -1.80 -2.11 4.33 3.19 7.98 -2.73 6.15 3.44 3.97 -0.86 -2.94 2.43 3.31 4.29 1.66
Mitotic Spindle 2.75 3.78 2.70 0.40 0.45 -2.87 -3.01 6.25 5.13 3.71 -2.02 -1.43 8.32 5.69 7.52
None -3.21 -4.51 0.95 3.36 3.63 -2.26 4.27 3.10 3.22 -0.25 -3.31 3.01 2.63 4.15 0.91
Nuclear Periphery -0.07 -5.38 -3.53 -3.35 0.82 7.27 -10.75 2.54 -5.05 11.13 4.31 -10.85 2.46 -1.91 11.81
Nuclear Periphery Foci -3.02 -2.16 -1.87 0.94 -0.28 8.57 -6.41 4.96 -3.82 8.09 4.75 -4.03 4.86 0.14 8.30
Nucleolus -4.93 -2.98 -3.59 1.60 -1.85 -2.30 -10.26 -3.97 1.31 6.29 -3.02 -9.96 -4.04 1.68 1.00
Nucleus -6.24 -12.26 -5.53 -2.16 0.45 -10.82 -5.67 -9.42 2.50 4.15 -12.22 -12.81 -10.87 1.32 5.13
Peroxisomes -0.22 4.99 -0.72 -0.63 -1.82 -1.33 2.79 -0.86 1.03 -1.89 -1.38 3.96 -0.85 0.97 -2.25
Vacuole -4.82 -4.01 -11.98 -9.82 -10.76 -0.03 -16.05 -16.11 -15.38 10.92 -4.57 -17.33 -17.41 -13.59 -1.47
Vacuole Periphery 11.09 16.88 15.94 7.39 2.55 16.01 6.35 24.47 8.13 6.11 17.42 17.15 30.24 14.30 14.22
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl34a

Rpl34a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl34a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available