Standard name
Human Ortholog
Description DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis; also known as glycerol-1-phosphatase; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; GPP2 has a paralog, GPP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.98 0.95 0.91 0.81 0.74 0.68 0.68 0.68 0.64 0.57 0.99 0.99 0.96 0.93 0.95 0.89 0.91 0.9 0.93 0.87 0.88 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0 0 0 0.13 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.08 0.19 0.22 0.27 0.2 0.24 0.31 0.3 0 0 0.05 0.05 0.1 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 2 1 2 2 5 0 0 0 0 0 0 0 0 2 0 1 0
Bud 0 1 0 0 0 0 2 0 3 0 2 3 0 0 0 1 1 0 4 13 7 7 8 7
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 2 1 2 3 8 8 2 10 10 6 1 1 3 1 1 3 0 0 0 0 0 0
Cytoplasm 173 363 351 265 168 305 331 242 254 282 228 213 144 204 210 176 191 182 165 346 328 178 155 202
Endoplasmic Reticulum 1 2 0 0 0 4 6 1 5 15 9 12 0 0 2 8 6 4 0 2 0 1 1 1
Endosome 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 2 1 0 1 0 2
Golgi 1 0 2 0 0 0 0 1 0 0 2 0 0 0 0 0 1 1 2 0 0 0 0 0
Mitochondria 5 1 1 4 12 10 19 10 47 17 19 25 0 0 0 7 1 3 1 1 5 1 1 1
Nucleus 0 0 0 0 3 6 15 11 11 21 8 15 0 0 0 1 2 2 0 3 1 4 3 1
Nuclear Periphery 1 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 1 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2 1 0 2
Vac/Vac Membrane 4 8 10 13 14 70 97 96 76 98 110 113 3 3 11 9 20 24 1 3 1 2 0 0
Unique Cell Count 182 373 359 278 185 377 446 357 373 416 358 371 145 207 219 189 201 205 181 385 354 206 177 224
Labelled Cell Count 187 378 367 283 199 398 481 372 399 447 390 393 148 208 226 204 223 219 181 385 354 206 177 224


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.8 7.3 8.3 7.2 6.5 9.6 10.2 11.0 11.8 12.6 15.3 13.9 16.3 17.1 16.2 19.7 24.7 26.3 13.1 13.8 11.0
Std Deviation (1e-4) 4.8 2.7 3.7 2.3 2.3 3.4 3.5 3.7 4.6 4.4 5.5 5.4 7.1 7.2 6.7 11.2 12.8 14.2 13.7 13.6 7.7
Intensity Change (Log2) – – – -0.21 -0.37 0.21 0.29 0.4 0.5 0.59 0.88 0.74 0.97 1.04 0.96 1.25 1.57 1.66 0.65 0.73 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 2.1 2.3 0 0 0 0 0 0 0
Cytoplasm -1.7 -3.7 -7.3 -9.2 -10.6 -10.6 -10.8 -11.6 -13.0 1.2 0.6 -1.3 -2.7 -1.8 -4.5
Endoplasmic Reticulum 0 0 0 0 0 0 3.6 0 3.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.7 3.6 2.7 6.7 3.5 4.1 4.7 0 0 0 0 0 0
Nucleus 0 0 0 3.5 3.4 3.3 4.3 0 3.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.3 2.6 6.9 7.9 9.1 7.4 8.3 10.0 10.0 0 0 1.4 1.2 3.6 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.225 8.9055 8.0627 6.8564 8.4998 8.3944 10.5129 11.9173 13.3799 10.871 11.358 12.3935 11.0098 12.1462 11.6288 10.8887 11.7632 11.3402
Actin 0.007 0.0014 0.0018 0.001 0.0015 0.0008 0.0079 0.0022 0.0187 0.0392 0.0042 0.0007 0.0091 0.0005 0.0212 0.015 0.0013 0.0025
Bud 0.005 0.0061 0.0023 0.001 0.0023 0.0011 0.0009 0.0046 0.0004 0.0017 0.0002 0.0002 0.0041 0.0029 0.0015 0.0004 0.0002 0.0009
Bud Neck 0.0006 0.0005 0.0005 0.0007 0.0006 0.0015 0.0038 0.0006 0.0007 0.0009 0.0008 0.001 0.0007 0.0003 0.0005 0.0007 0.0007 0.0016
Bud Periphery 0.0025 0.0028 0.0009 0.0004 0.0021 0.0004 0.0007 0.0022 0.0002 0.0008 0.0001 0.0001 0.0031 0.0011 0.0012 0.0003 0.0002 0.0018
Bud Site 0.004 0.0139 0.0042 0.0007 0.0005 0.0002 0.0208 0.0106 0.0035 0.0011 0.0007 0.0001 0.0009 0.0039 0.003 0.0007 0.0001 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0.0002 0 0.0005 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0001 0 0.0001
Cytoplasm 0.9198 0.9417 0.9411 0.9469 0.9142 0.9295 0.8684 0.8721 0.9392 0.829 0.7026 0.9494 0.8843 0.9472 0.9232 0.9292 0.9409 0.9274
Cytoplasmic Foci 0.0066 0.0031 0.0059 0.0069 0.0051 0.007 0.0168 0.0086 0.0059 0.0236 0.0782 0.0039 0.0122 0.0012 0.0032 0.0087 0.0017 0.0068
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0.002 0 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0033 0.0014 0.0029 0.0036 0.0075 0.0026 0.0036 0.0016 0.0018 0.0018 0.0157 0.0019 0.0041 0.0006 0.0027 0.0021 0.0027 0.0017
Endosome 0.0074 0.0009 0.0024 0.0036 0.0023 0.004 0.02 0.0038 0.0048 0.0075 0.0876 0.001 0.0085 0.0002 0.004 0.0043 0.01 0.0028
Golgi 0.0019 0.0001 0.0002 0.0002 0.0002 0.0003 0.0081 0.0008 0.0091 0.0193 0.0255 0.0001 0.0032 0 0.0017 0.0048 0.0006 0.0004
Lipid Particles 0.0011 0 0.0001 0.0001 0 0.0002 0.0086 0.0005 0.0003 0.002 0.0105 0 0.0058 0 0.0017 0.0038 0.0005 0.0002
Mitochondria 0.0014 0.0003 0.0003 0.0005 0.0003 0.0024 0.008 0.0011 0.0032 0.007 0.0042 0.0001 0.0015 0.0001 0.0067 0.0039 0.0026 0.0002
None 0.0176 0.0105 0.0174 0.0107 0.0381 0.0109 0.008 0.0598 0.0021 0.0375 0.018 0.0266 0.0365 0.0338 0.0141 0.0044 0.0022 0.0195
Nuclear Periphery 0.0034 0.0023 0.003 0.004 0.0051 0.0035 0.0077 0.0034 0.0007 0.0016 0.0182 0.0014 0.0034 0.0006 0.002 0.0019 0.0184 0.0025
Nucleolus 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0002 0.001 0 0.0003 0.0004 0 0.0002 0 0.0001 0 0.0003 0
Nucleus 0.0134 0.0123 0.0128 0.013 0.0151 0.0294 0.0049 0.0131 0.007 0.019 0.0103 0.0114 0.0107 0.0066 0.008 0.0064 0.0132 0.0294
Peroxisomes 0.0007 0.0002 0.0002 0.0003 0.0002 0.0001 0.0054 0.0011 0.0015 0.0042 0.0061 0.0001 0.0048 0 0.0021 0.0113 0.0001 0.0005
Punctate Nuclear 0.001 0.001 0.0022 0.0029 0.001 0.0043 0.0019 0.0109 0.0004 0.002 0.013 0.0015 0.0034 0.0003 0.0014 0.0012 0.0014 0.0014
Vacuole 0.0026 0.001 0.0013 0.0027 0.003 0.0011 0.0016 0.0014 0.0003 0.0011 0.0025 0.0004 0.0011 0.0003 0.001 0.0007 0.0013 0.0003
Vacuole Periphery 0.0003 0.0001 0.0002 0.0005 0.0007 0.0004 0.002 0.0004 0.0001 0.0003 0.0009 0.0001 0.0003 0 0.0004 0.0001 0.0015 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 52.0197 26.1986 29.1607 45.287 46.5471 46.8651 44.3697 49.1659 73.7263 58.9741
Translational Efficiency 1.2217 1.6007 1.6857 1.5692 1.421 1.6821 1.5928 1.7836 1.7234 1.6191

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1763 298 203 227 1209 2348 263 1864 2972 2646 466 2091

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 740.94 966.97 1277.21 3811.19 936.93 915.21 1644.66 2449.69 820.67 921.04 1484.59 2597.50
Standard Deviation 128.68 205.51 255.98 1878.90 229.69 156.95 527.97 1232.48 201.38 163.96 468.04 1384.46
Intensity Change Log 2 — 0.384114 0.785567 2.362813 — -0.033838 0.811776 1.386586 — 0.165774 0.800261 1.899735

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000135 0.000285 0.000800 0.000222 0.000152 0.000168 0.000564 0.000161 0.000142 0.000181 0.000667 0.000168
Bud Neck 0.007075 0.017428 0.002727 0.004581 0.001417 0.014177 0.002889 0.001620 0.004774 0.014543 0.002818 0.001942
Bud Site 0.008106 0.019584 0.005991 0.014068 0.003055 0.013245 0.007713 0.006297 0.006051 0.013959 0.006963 0.007140
Cell Periphery 0.000121 0.000126 0.000310 0.001678 0.000143 0.000108 0.000302 0.000214 0.000130 0.000110 0.000305 0.000373
Cytoplasm 0.642013* 0.615063* 0.518827* 0.647940* 0.785432* 0.700586* 0.578132* 0.778677* 0.700355* 0.690954* 0.552297* 0.764484*
Cytoplasmic Foci 0.160197 0.156125 0.013295 0.033434 0.058425 0.105549 0.015560 0.010396 0.118796 0.111245 0.014573 0.012897
Eisosomes 0.000082 0.000057 0.000046 0.000007 0.000024 0.000054 0.000046 0.000002 0.000058 0.000055 0.000046 0.000002
Endoplasmic Reticulum 0.000985 0.000732 0.003596 0.000081 0.001204 0.000346 0.002147 0.000044 0.001074 0.000389 0.002778 0.000048
Endosome 0.006619 0.012368 0.004491 0.017683 0.003671 0.007130 0.003357 0.000223 0.005420 0.007720 0.003851 0.002118
Golgi 0.001940 0.003339 0.000383 0.009700 0.000354 0.001787 0.000293 0.000293 0.001295 0.001962 0.000332 0.001314
Lipid Particles 0.003455 0.001371 0.002281 0.002697 0.001045 0.001175 0.001830 0.000346 0.002475 0.001197 0.002026 0.000601
Mitochondria 0.002286 0.000549 0.000316 0.000557 0.000154 0.002894 0.000246 0.000244 0.001419 0.002630 0.000276 0.000278
Mitotic Spindle 0.002744 0.015088 0.013953 0.010916 0.001317 0.008258 0.005383 0.018713 0.002163 0.009027 0.009117 0.017866
None 0.009452 0.001900 0.003594 0.009755 0.012536 0.003973 0.010765 0.014541 0.010706 0.003740 0.007641 0.014021
Nuclear Periphery 0.000245 0.000203 0.001295 0.000364 0.000203 0.000147 0.000815 0.000309 0.000228 0.000153 0.001024 0.000315
Nuclear Periphery Foci 0.000937 0.001368 0.006268 0.001644 0.002883 0.000368 0.006053 0.000797 0.001728 0.000480 0.006147 0.000889
Nucleolus 0.000794 0.000387 0.000243 0.000107 0.000437 0.000558 0.000381 0.000177 0.000649 0.000539 0.000321 0.000169
Nucleus 0.101682 0.070636 0.270359* 0.196632* 0.095824 0.091466 0.170218* 0.154935* 0.099299 0.089120 0.213841* 0.159462*
Peroxisomes 0.002697 0.003984 0.000219 0.001471 0.000349 0.002800 0.000208 0.000049 0.001742 0.002933 0.000212 0.000203
Vacuole 0.047489 0.078634 0.150747 0.043393 0.030993 0.044130 0.192880 0.010147 0.040779 0.048016 0.174526 0.013756
Vacuole Periphery 0.000947 0.000772 0.000259 0.003072 0.000382 0.001081 0.000221 0.001816 0.000717 0.001046 0.000238 0.001953

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.93 -9.61 -3.69 0.17 7.64 -0.25 -8.42 -1.06 -0.82 6.35 -1.39 -12.64 -1.87 -0.78 9.50
Bud Neck -2.36 4.13 3.50 3.01 -0.25 -12.35 -6.72 -2.79 11.63 5.46 -9.28 -0.51 6.57 11.83 6.26
Bud Site -2.83 -0.82 -0.56 2.56 0.10 -9.55 -8.01 -4.58 6.06 4.18 -6.85 -4.72 -0.37 6.58 4.34
Cell Periphery -0.19 -7.10 -4.51 -4.50 -3.73 3.32 -6.33 -7.85 -9.71 2.47 2.53 -9.45 -7.40 -7.81 -1.02
Cytoplasm 1.45 11.90 1.77 0.20 -8.10 10.70 19.31 8.20 -3.42 -16.16 1.28 20.45 -1.95 -3.12 -21.46
Cytoplasmic Foci 0.93 31.08 19.19 9.45 -4.93 -11.28 10.61 15.07 28.82 2.28 1.87 30.35 33.17 30.05 1.06
Eisosomes 2.22 1.84 7.01 6.89 8.61 -10.33 -2.55 12.95 21.44 8.36 0.66 1.79 8.79 22.52 12.43
Endoplasmic Reticulum 1.64 -6.93 7.28 7.15 9.27 5.24 -1.85 7.57 4.50 7.97 6.08 -6.74 10.51 5.71 11.72
Endosome -2.64 2.94 2.27 3.56 1.19 -4.45 2.86 10.03 10.64 9.29 -3.09 5.15 14.95 11.17 9.31
Golgi -0.57 6.06 0.31 0.65 -1.31 -4.84 0.13 2.15 5.74 4.05 -1.71 5.96 5.21 4.71 0.38
Lipid Particles 6.91 0.59 -1.57 -4.25 -1.81 -0.74 -5.27 1.43 2.24 6.00 7.41 -1.48 6.07 -0.66 6.70
Mitochondria 4.48 5.30 5.71 1.85 3.05 -4.59 -4.19 -2.80 4.50 2.73 -2.09 5.02 5.57 4.56 4.80
Mitotic Spindle -2.01 -1.66 -1.63 1.12 0.74 -4.59 -3.97 -7.37 -2.01 -0.82 -4.42 -3.31 -6.81 -1.52 -0.41
None 7.47 6.23 2.07 -6.18 -4.64 5.93 2.02 1.17 -14.58 -1.59 8.08 4.04 0.39 -15.55 -4.95
Nuclear Periphery 0.53 -14.33 -6.45 -6.79 10.35 3.25 -13.44 -6.35 -9.11 10.79 3.82 -19.23 -6.65 -10.36 15.73
Nuclear Periphery Foci -2.78 -7.62 -5.60 -3.27 4.87 9.51 -4.87 5.46 -8.08 8.28 9.24 -9.72 1.26 -8.61 10.22
Nucleolus 5.31 8.16 10.77 4.77 5.45 -0.25 0.89 1.90 7.28 3.09 1.50 4.23 6.19 7.80 4.00
Nucleus 4.90 -11.02 -5.95 -8.16 5.93 1.35 -9.94 -17.18 -20.89 1.93 3.43 -14.61 -19.76 -22.39 5.42
Peroxisomes -1.12 5.42 5.06 4.65 0.17 -8.49 2.20 3.63 10.22 7.28 -3.15 5.73 6.30 11.18 6.03
Vacuole -5.48 -11.90 -7.36 -3.69 4.95 -4.46 -13.60 -4.72 -0.92 12.76 -3.52 -17.80 -5.45 -2.64 16.29
Vacuole Periphery 1.37 7.55 1.10 0.45 -0.66 -1.78 1.78 -0.34 1.04 -0.81 -0.92 7.27 0.58 1.08 -0.83
Standard name
Human Ortholog
Description DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis; also known as glycerol-1-phosphatase; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; GPP2 has a paralog, GPP1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gpp2

Gpp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available