Standard name
Human Ortholog
Description Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.52 0.92 0.95 0.93 0.88 0.72 0.67 0.64 0.47 0.4 0.32 0.33 0.81 0.85 0.89 0.89 0.92 – 0.34 0.71 0.65 0.7 0.7 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 – 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0.06 0 0 0 0 0 0.08 0.08 0.07 0 0 – 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.14 0.05 0 0.06 0 0.33 0.19 0.31 0.51 0.51 0.6 0.61 0 0 0 0.08 0 – 0.22 0.06 0.15 0.1 0.08 0.05
Nucleus 0 0 0 0 0 0 0 0.05 0.07 0.08 0.14 0.13 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0.05 0.05
Vac/Vac Membrane 0.3 0.06 0 0 0.13 0 0.13 0.09 0.11 0.09 0.12 0.13 0.14 0.16 0.06 0.08 0.09 – 0.17 0.08 0 0.05 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 2 4 6 3 7 3
Bud 0 1 1 1 1 3 2 5 5 5 4 1 0 0 0 0 0 – 1 2 4 6 13 19
Bud Neck 0 1 1 2 4 3 2 3 3 3 2 0 1 0 0 1 0 – 0 1 1 3 5 3
Bud Site 0 0 0 0 0 0 0 1 3 0 1 3 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 2 0 – 0 0 2 1 2 2
Cytoplasm 74 271 294 184 240 174 210 254 114 115 135 124 187 317 394 304 252 – 70 234 217 275 387 366
Endoplasmic Reticulum 4 1 2 1 0 0 0 0 0 0 0 0 3 0 0 20 15 – 3 0 7 2 6 5
Endosome 12 2 1 3 5 1 18 15 2 7 8 8 19 29 30 6 3 – 11 4 7 3 11 4
Golgi 0 0 3 1 0 0 0 0 0 0 0 0 2 0 0 0 0 – 1 1 3 5 0 3
Mitochondria 20 15 2 11 3 79 59 123 125 146 251 226 5 2 0 26 4 – 46 21 48 40 46 24
Nucleus 3 1 2 1 1 4 11 18 16 24 60 47 0 2 3 3 2 – 7 5 3 3 3 7
Nuclear Periphery 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 – 3 0 1 0 0 4
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 – 0 0 0 0 3 6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – 1 1 0 0 0 0
SpindlePole 1 0 3 2 7 2 14 9 7 8 11 7 1 1 0 2 0 – 12 10 6 15 29 27
Vac/Vac Membrane 43 17 11 7 36 7 42 35 27 25 50 47 33 60 28 27 26 – 36 25 8 18 20 28
Unique Cell Count 143 293 310 198 272 241 314 399 243 285 417 372 230 374 442 342 274 208 329 333 392 554 523
Labelled Cell Count 157 310 320 213 297 274 358 465 302 335 523 463 251 412 456 391 302 208 329 333 392 554 523


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.9 5.0 4.9 5.3 4.0 4.4 4.5 4.0 4.1 3.8 4.1 4.6 4.8 4.7 4.9 5.3 4.8 3.9 4.0 4.2
Std Deviation (1e-4) 0.8 0.8 1.6 2.2 1.7 1.9 1.5 1.8 1.8 1.4 1.0 1.0 1.3 1.6 1.3 1.1 1.5 1.2 1.7 1.5 1.5
Intensity Change (Log2) – – – -0.04 0.07 -0.31 -0.2 -0.16 -0.34 -0.29 -0.39 -0.31 -0.12 -0.07 -0.1 -0.04 0.08 -0.08 -0.35 -0.32 -0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 2.4 2.9 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6304 -0.432 -0.6376 0.1008 -0.4712 -0.5656 0.5898 0.652 0.5917 0.7445 0.5088 0.5371 2.5273 3.4029 3.0178 3.1403 2.8968 3.0553
Actin 0.0122 0.0003 0.005 0.028 0.0392 0.0028 0.0432 0.0005 0.0028 0.0243 0.0148 0.0107 0.0006 0.0001 0.0014 0.0012 0.0005 0.0004
Bud 0.0015 0.0001 0.0004 0.0104 0.0003 0.0015 0.0019 0.0013 0.0057 0.0028 0.0008 0.0021 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.0023 0.0006 0.0012 0.0022 0.0022 0.0054 0.003 0.001 0.0039 0.0023 0.0035 0.0048 0.0013 0.0006 0.0011 0.001 0.0009 0.0043
Bud Periphery 0.0005 0.0001 0.0006 0.0038 0.0004 0.0005 0.0023 0.001 0.0022 0.0027 0.0022 0.0014 0.0001 0.0002 0.0002 0.0001 0 0.0001
Bud Site 0.0013 0.0014 0.0016 0.0031 0.0009 0.0002 0.0027 0.0072 0.0079 0.0065 0.0008 0.0005 0.003 0.0016 0.0016 0.0003 0.0002 0.0002
Cell Periphery 0.0001 0 0.0001 0.0005 0.0001 0 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0 0.0001 0.0001 0 0 0
Cytoplasm 0.0981 0.1272 0.0722 0.0622 0.0761 0.0891 0.0828 0.1644 0.083 0.0425 0.0784 0.0717 0.1434 0.1658 0.1319 0.1338 0.1707 0.172
Cytoplasmic Foci 0.0213 0.0067 0.0073 0.0184 0.0098 0.0093 0.0184 0.0062 0.01 0.0064 0.0426 0.0059 0.0074 0.0047 0.0063 0.0068 0.0057 0.0065
Eisosomes 0.0004 0.0001 0.0001 0.0008 0.0003 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0001 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0032 0.0026 0.0023 0.0072 0.006 0.0014 0.0086 0.0023 0.0017 0.0017 0.0029 0.0024 0.0023 0.0023 0.0024 0.0024 0.0024 0.0022
Endosome 0.01 0.0034 0.0048 0.0086 0.0144 0.0044 0.0178 0.0039 0.0075 0.0069 0.0063 0.0064 0.0036 0.0044 0.0025 0.0034 0.0047 0.0034
Golgi 0.0028 0.0001 0.0009 0.0017 0.0061 0.0011 0.0054 0.0005 0.0021 0.0046 0.0028 0.0035 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001
Lipid Particles 0.0062 0.0006 0.0014 0.0022 0.0184 0.0014 0.0161 0.001 0.0041 0.0056 0.0119 0.0017 0.0004 0.0002 0.0004 0.0004 0.0002 0.0003
Mitochondria 0.0023 0.0005 0.0023 0.003 0.0043 0.0009 0.0043 0.0026 0.0037 0.0024 0.0052 0.0117 0.0004 0.0006 0.0007 0.0008 0.0006 0.0006
None 0.2241 0.3053 0.301 0.2807 0.2378 0.1826 0.1759 0.2944 0.2358 0.2528 0.1639 0.1667 0.2662 0.2924 0.3429 0.2322 0.4208 0.1897
Nuclear Periphery 0.0198 0.0201 0.0162 0.0121 0.0348 0.0122 0.021 0.0101 0.0086 0.0388 0.017 0.0176 0.0092 0.0093 0.0094 0.0256 0.0111 0.0081
Nucleolus 0.0075 0.0084 0.0061 0.0082 0.0084 0.0096 0.0112 0.0066 0.0065 0.0093 0.0133 0.0096 0.0034 0.0029 0.0032 0.0043 0.0041 0.005
Nucleus 0.4754 0.438 0.4805 0.4599 0.4206 0.5841 0.4953 0.4151 0.5128 0.4772 0.4729 0.5473 0.4842 0.4624 0.4541 0.4942 0.3326 0.5381
Peroxisomes 0.0023 0.0002 0.0014 0.0007 0.0171 0.0005 0.0158 0.0011 0.0086 0.0025 0.0301 0.0009 0.0003 0.0001 0.0003 0.0003 0.0002 0.0001
Punctate Nuclear 0.1068 0.0822 0.0927 0.0841 0.1011 0.0915 0.0701 0.0776 0.091 0.1081 0.128 0.133 0.0716 0.0394 0.0394 0.0918 0.0434 0.067
Vacuole 0.0013 0.0018 0.0013 0.0019 0.001 0.0008 0.0024 0.0027 0.0016 0.0014 0.0014 0.001 0.002 0.0119 0.0013 0.0006 0.0013 0.0015
Vacuole Periphery 0.0006 0.0004 0.0005 0.0005 0.0008 0.0003 0.0011 0.0005 0.0003 0.0008 0.0009 0.0008 0.0003 0.0004 0.0002 0.0003 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.9865 46.5413 36.7638 46.1707 56.6148 48.6773 38.7089 56.35 62.8395 52.1444
Translational Efficiency 2.0313 1.9305 2.4223 2.3972 2.1114 1.7411 2.1238 1.8923 1.8376 1.9179

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
182 1484 187 1148 1934 2225 311 2243 2116 3709 498 3391

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 625.69 737.69 855.40 766.80 611.29 704.47 837.33 839.30 612.53 717.76 844.12 814.76
Standard Deviation 86.12 81.88 86.75 102.17 66.59 86.69 87.76 106.92 68.61 86.35 87.82 110.78
Intensity Change Log 2 — 0.237567 0.451151 0.293402 — 0.204681 0.453939 0.457330 — 0.221409 0.452530 0.376740

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000618 0.000347 0.000523 0.000491 0.000047 0.000202 0.000409 0.000616 0.000096 0.000260 0.000452 0.000573
Bud Neck 0.099417 0.015034 0.001500 0.010163 0.007131 0.027729 0.001342 0.017900 0.015069 0.022649 0.001401 0.015281
Bud Site 0.079581 0.007265 0.000920 0.034101 0.001067 0.009422 0.001216 0.029522 0.007820 0.008559 0.001105 0.031072
Cell Periphery 0.001271 0.000141 0.000138 0.000278 0.000089 0.000624 0.000121 0.000199 0.000191 0.000430 0.000127 0.000225
Cytoplasm 0.188385* 0.377465* 0.079796 0.209664* 0.402516* 0.333261* 0.110741* 0.282853* 0.384098* 0.350947* 0.099121 0.258076*
Cytoplasmic Foci 0.102770 0.152197 0.005593 0.009663 0.121328 0.084146 0.006318 0.004052 0.119731 0.111373 0.006046 0.005951
Eisosomes 0.000247 0.000082 0.000078 0.000056 0.000078 0.000143 0.000055 0.000026 0.000093 0.000119 0.000064 0.000036
Endoplasmic Reticulum 0.003508 0.002017 0.007227 0.004336 0.000590 0.001400 0.005663 0.001914 0.000841 0.001647 0.006250 0.002734
Endosome 0.018346 0.008036 0.001733 0.006618 0.002810 0.008339 0.001447 0.002062 0.004146 0.008217 0.001554 0.003605
Golgi 0.003043 0.002856 0.000079 0.001807 0.000741 0.001802 0.000063 0.001050 0.000939 0.002224 0.000069 0.001306
Lipid Particles 0.013104 0.008403 0.002565 0.001812 0.007477 0.010265 0.002083 0.000514 0.007961 0.009520 0.002264 0.000954
Mitochondria 0.013744 0.011779 0.000552 0.002172 0.003129 0.012216 0.000452 0.002089 0.004042 0.012041 0.000490 0.002117
Mitotic Spindle 0.005993 0.005361 0.000278 0.033437 0.000383 0.005223 0.000291 0.030481 0.000866 0.005278 0.000286 0.031482
None 0.023421 0.007244 0.004968 0.006050 0.005514 0.017673 0.006364 0.009506 0.007054 0.013500 0.005840 0.008336
Nuclear Periphery 0.000724 0.001583 0.002026 0.002929 0.000895 0.001940 0.001700 0.002278 0.000880 0.001797 0.001823 0.002498
Nuclear Periphery Foci 0.001698 0.000880 0.000998 0.001265 0.000268 0.000556 0.001368 0.000324 0.000391 0.000686 0.001229 0.000643
Nucleolus 0.017728 0.003437 0.002680 0.001711 0.001534 0.005815 0.001020 0.000891 0.002927 0.004863 0.001643 0.001168
Nucleus 0.370338* 0.359249* 0.854709* 0.624720* 0.426133* 0.442601* 0.831505* 0.578532* 0.421334* 0.409251* 0.840218* 0.594168*
Peroxisomes 0.014888 0.011850 0.000409 0.000698 0.002742 0.007124 0.000258 0.000532 0.003787 0.009015 0.000315 0.000588
Vacuole 0.038560 0.023445 0.033046 0.045317 0.015258 0.027845 0.027471 0.032808 0.017262 0.026085 0.029564 0.037043
Vacuole Periphery 0.002617 0.001330 0.000182 0.002713 0.000271 0.001676 0.000114 0.001852 0.000473 0.001537 0.000140 0.002143

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.64 -0.62 0.70 -2.26 2.21 -3.44 -8.84 -4.57 -2.44 1.94 -4.29 -10.26 -6.14 -2.94 2.75
Bud Neck 5.31 6.14 5.65 3.25 -8.24 -11.18 6.84 -7.18 6.12 -13.25 -4.02 7.94 0.66 6.58 -15.43
Bud Site 5.23 5.65 4.13 -4.60 -6.68 -7.30 -2.66 -10.03 -4.30 -9.18 -0.59 4.41 -5.88 -6.35 -11.35
Cell Periphery 4.16 4.11 3.89 -3.15 -2.29 -7.20 -3.95 -4.71 6.45 0.25 -4.72 1.59 0.96 5.81 -1.19
Cytoplasm -10.37 6.25 0.72 21.44 -9.61 8.54 27.45 22.48 14.21 -11.53 4.68 33.31 24.96 23.91 -15.02
Cytoplasmic Foci -3.91 8.60 8.31 29.09 -2.39 7.90 28.52 31.66 28.53 1.94 1.91 30.81 32.33 39.99 0.63
Eisosomes 4.45 3.90 4.90 3.54 3.49 -5.30 0.02 11.93 9.34 6.65 -3.13 0.89 9.87 10.24 7.56
Endoplasmic Reticulum 1.45 -5.33 -2.47 -11.80 4.61 -5.70 -11.60 -20.03 -9.92 7.68 -6.24 -13.99 -20.51 -15.01 8.72
Endosome 3.06 4.90 4.26 3.76 -2.10 -8.02 2.31 4.50 10.61 2.35 -6.72 4.70 3.90 10.70 -0.30
Golgi 0.21 3.78 2.50 2.63 -1.77 -2.37 2.55 0.87 3.11 -1.28 -3.00 3.44 1.16 4.11 -2.10
Lipid Particles 1.85 4.32 4.69 13.43 2.38 -3.02 7.54 12.63 13.64 4.85 -2.16 9.05 12.86 18.24 5.57
Mitochondria 0.59 4.01 3.62 8.14 -6.25 -8.23 5.68 2.59 10.09 -12.35 -8.69 6.88 4.17 12.97 -13.30
Mitotic Spindle 0.21 1.46 -4.22 -4.96 -5.57 -4.89 -1.19 -9.82 -6.97 -9.72 -5.96 -0.42 -11.57 -8.58 -10.88
None 2.54 3.08 2.80 1.99 -2.44 -7.72 0.12 -3.15 5.96 -2.42 -5.41 2.38 -0.21 6.04 -3.25
Nuclear Periphery -5.74 -8.44 -15.05 -10.39 -4.52 -5.72 -10.50 -17.62 -8.24 -7.37 -7.33 -12.91 -22.55 -12.53 -8.47
Nuclear Periphery Foci 0.90 -0.07 0.28 -2.83 0.71 -2.51 -6.23 -1.80 1.75 5.95 -2.98 -6.50 -3.55 -0.13 5.28
Nucleolus 2.82 3.01 3.21 5.37 1.43 -9.04 2.45 2.42 9.91 -0.14 -3.68 2.56 3.55 11.35 1.25
Nucleus 0.52 -15.37 -10.22 -24.48 10.02 -1.74 -27.20 -22.97 -20.85 12.15 1.65 -34.49 -26.82 -31.71 15.81
Peroxisomes 0.82 4.14 4.08 10.61 -1.13 -5.54 9.62 9.52 9.13 0.39 -7.18 9.00 8.77 13.93 -0.49
Vacuole 1.88 -2.32 -5.42 -12.31 -1.88 -6.86 -6.01 -15.41 -10.69 -1.04 -5.82 -7.58 -19.15 -16.19 -1.88
Vacuole Periphery 2.64 5.10 1.83 0.05 -2.01 -9.17 5.10 -1.89 2.70 -2.36 -9.32 6.33 -1.88 1.99 -3.08
Standard name
Human Ortholog
Description Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation
Localization
Cell Percentages nucleus (28%), cytoplasm (6%), mixed (49%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tho1

Tho1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tho1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available