Standard name
Human Ortholog
Description Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.1 0.14 0 0 0 0.05 0.06 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.2 0.13 0.17 0 0 0 0 0 0 0 0 0 0 0.65 0.74 0.57 0.09 0.1 0.09 0.1 0.06 0.06
Mitochondria 0.9 0.9 0.86 0.92 0.82 0.8 0.82 0.8 0.91 0.89 0.9 0.87 0.95 0.92 0.91 0.12 0.09 0.12 0.73 0.71 0.77 0.7 0.63 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.06 0 0 0 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.19 0.12 0 0 0.09 0.12 0.1 0.11 0.14 0.08 0.12 0.1 0.1 0.06 0.07 0.1 0.09 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.13 0.12
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0.1 0 0.06 0.08 0 0.07 0 0 0 0.05 0.05 0.06 0 0.16 0.14 0.16 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 9 1 5 1 4 3 0 2 2 0 6 1 6 28 19 27 3 6 5 10 23 22
Bud 0 3 0 4 1 2 13 8 7 3 8 3 3 3 5 1 1 1 0 2 1 1 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Bud Site 0 2 0 0 0 1 1 0 5 2 0 1 0 0 0 1 0 1 – – – – – –
Cell Periphery 0 1 8 2 3 1 3 0 2 0 0 2 3 3 5 9 8 2 0 0 0 0 1 2
Cytoplasm 4 0 5 6 9 14 12 10 9 8 7 5 2 6 9 10 3 6 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 2 1 3 2 0 0 0 1 0 0 2 5 1 5 0 0 1 0 1 2
Endosome 3 0 2 2 3 3 6 6 0 0 0 1 0 1 0 0 1 5 3 1 3 1 9 6
Golgi 0 4 39 25 40 4 9 10 0 0 0 1 7 5 6 135 139 110 11 14 20 18 23 20
Mitochondria 112 114 165 172 192 192 298 253 243 232 163 181 350 331 399 25 16 24 92 97 168 134 242 239
Nucleus 1 1 0 0 0 2 4 1 1 4 0 1 1 0 1 0 2 3 0 0 0 0 0 0
Nuclear Periphery 5 2 0 1 6 1 23 13 11 6 14 13 1 1 3 1 0 1 1 0 0 0 4 3
Nucleolus 24 15 5 4 20 29 37 34 38 21 22 21 38 23 31 20 16 18 3 2 0 1 5 6
Peroxisomes 0 2 8 0 8 3 12 7 4 3 3 6 4 12 16 3 1 5 4 4 8 19 51 41
SpindlePole 1 0 1 0 1 2 6 6 3 1 2 3 0 1 0 0 0 2 1 0 0 1 3 1
Vac/Vac Membrane 4 8 19 8 14 20 16 21 5 10 3 11 20 20 18 33 27 32 2 6 6 2 8 6
Unique Cell Count 125 126 192 187 235 240 363 317 266 260 181 208 368 361 438 208 188 194 127 137 220 193 386 363
Labelled Cell Count 154 152 261 225 304 276 447 374 328 292 224 250 435 407 501 271 234 242 127 137 220 193 386 363


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.9 18.6 19.6 21.9 21.4 17.4 16.4 14.9 12.9 11.8 11.2 12.6 26.6 25.4 24.3 34.4 34.5 33.0 20.0 20.6 19.5
Std Deviation (1e-4) 6.1 5.0 5.3 6.1 6.3 6.1 5.3 4.6 4.1 3.5 3.6 4.3 6.8 6.5 6.6 12.0 10.0 11.8 5.0 5.4 5.2
Intensity Change (Log2) – – – 0.16 0.13 -0.17 -0.26 -0.4 -0.61 -0.74 -0.81 -0.64 0.44 0.37 0.31 0.81 0.81 0.75 0.03 0.07 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.5 0 0 0 0 0 -2.0 0 -2.1 0 0 3.0 2.0 3.1
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0
Cytoplasm 0.4 0.7 1.6 0.5 0.4 0.5 0.3 0.7 0 0 0 0 1.2 0 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -1.8 -0.9 -6.4 -7.1 -6.4 -7.7 -7.6 -6.4 -6.6 -7.5 -7.8 -8.5 9.0 10.5 7.3
Mitochondria 1.9 -1.2 -1.6 -1.2 -1.7 1.8 1.1 1.2 0.3 3.8 2.1 1.9 -14.8 -15.1 -14.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 3.6 0 0 0 3.9 3.5 0 0 0 0 0 0
Nucleolus 0 2.6 3.6 3.2 3.3 4.2 2.5 3.6 3.0 3.3 1.9 2.2 2.9 2.5 2.8
Peroxisomes 0 -0.4 0 -0.5 -1.3 -1.8 0 -1.4 -0.7 0 -0.5 -0.3 -1.7 0 -0.9
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 -1.5 -0.6 -2.5 -1.3 -3.8 -2.6 -3.4 -1.7 -2.0 -1.9 -2.8 1.8 1.3 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 58.788 59.0815 60.0047 57.0128 61.2051 57.5323 66.8718 70.4886 67.9357 60.1286 58.4279 66.3399 60.121 61.4533 60.6676 47.9478 56.4661 62.5203
Actin 0.0086 0.001 0.0028 0.0202 0 0.0001 0.0158 0.0009 0.0011 0.0161 0.0003 0 0.0004 0.0009 0.0011 0 0 0.0001
Bud 0 0.0002 0.0001 0.0001 0 0 0.0001 0 0 0.0002 0 0 0.0001 0.0001 0 0 0 0
Bud Neck 0.0004 0 0.0001 0.0009 0 0 0.0002 0 0 0 0 0 0 0.0001 0 0 0 0
Bud Periphery 0.0001 0.0005 0.0002 0.0001 0 0 0.0003 0 0.0001 0.0005 0 0 0.0001 0 0 0 0 0
Bud Site 0.0002 0.0005 0.0009 0.0004 0 0 0.0003 0 0.0003 0.0002 0 0 0.0004 0.0005 0 0 0 0
Cell Periphery 0.0002 0.0003 0.0002 0.0002 0 0.0001 0.0003 0.0002 0.0002 0.0001 0.0003 0 0.0005 0.0003 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0004 0 0 0.0044 0 0 0.0007 0 0.0005 0.0001 0 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasmic Foci 0.0052 0 0.0003 0.0033 0 0.001 0.0119 0.0001 0.0079 0.0027 0.001 0 0.0061 0.0028 0.0048 0.0001 0.0022 0
Eisosomes 0.0006 0.0003 0.0008 0.0015 0.0001 0.0002 0.002 0.0025 0.0014 0.0004 0.0008 0.0002 0.0006 0.0003 0.0003 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0006 0 0 0.0042 0 0 0.001 0 0.0001 0.0002 0 0 0 0 0.0001 0 0 0
Endosome 0.003 0.0001 0.0001 0.0062 0 0 0.0061 0.0001 0.0021 0.0109 0 0 0.0002 0.0006 0.0008 0.0001 0.0001 0
Golgi 0.0054 0.0002 0.0003 0.0026 0.0001 0.0001 0.0052 0.0004 0.0006 0.0074 0 0.0001 0.0002 0.0014 0.0003 0.0002 0.0001 0.0001
Lipid Particles 0.0096 0.0067 0.0043 0.0047 0.0026 0.0047 0.0098 0.0034 0.0053 0.0079 0.0048 0.001 0.0057 0.0058 0.0033 0.0032 0.0036 0.0015
Mitochondria 0.9391 0.9628 0.9786 0.9268 0.9929 0.9435 0.9252 0.9847 0.9545 0.9358 0.9768 0.9968 0.9469 0.9686 0.9686 0.9879 0.9758 0.9897
None 0.0017 0 0 0.0094 0 0.0001 0.001 0 0.0008 0.0003 0.0001 0 0.0006 0 0.0002 0 0 0
Nuclear Periphery 0.0008 0.0001 0 0.004 0 0.0001 0.0026 0 0.0006 0.0004 0 0 0.0001 0 0.0012 0 0 0
Nucleolus 0.0068 0.0002 0.0003 0.0005 0.0001 0.0107 0.0021 0.0002 0.0062 0.0001 0.0089 0 0.0221 0.0007 0.004 0.0028 0.0141 0.0006
Nucleus 0.0004 0.0001 0 0.0011 0 0.0003 0.0005 0 0.0011 0.0001 0.0003 0 0.0016 0.0001 0.0004 0.0003 0.0006 0
Peroxisomes 0.0078 0.001 0.005 0.0017 0.0002 0.0006 0.0055 0.0036 0.0025 0.0101 0.0017 0.0002 0.0021 0.0025 0.0009 0.0004 0.0006 0.0002
Punctate Nuclear 0.0012 0 0 0.0062 0 0 0.0006 0 0.0006 0.0009 0 0 0.0006 0 0.0003 0 0.0001 0
Vacuole 0.0008 0.0012 0.0004 0.0002 0.0003 0.0007 0.0012 0.0003 0.0018 0.0019 0.0009 0 0.0017 0.001 0.0012 0.0006 0.0006 0.0002
Vacuole Periphery 0.0069 0.0249 0.0054 0.0012 0.0035 0.0378 0.0077 0.0036 0.0122 0.0038 0.0038 0.0016 0.0098 0.0141 0.0119 0.0042 0.0019 0.0071

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.0394 26.2577 23.1553 37.8052 42.4989 86.3685 92.7296 88.5556 85.3338 94.0818
Translational Efficiency 0.9257 0.9488 0.9911 0.7279 0.7941 0.9985 0.8717 0.7417 0.79 0.791

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2023 1013 431 1353 1406 1684 1489 186 3429 2697 1920 1539

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1688.25 1948.75 3300.18 1833.53 2016.13 2053.69 2411.80 2011.12 1822.69 2014.27 2611.22 1854.99
Standard Deviation 376.21 552.78 845.71 482.02 497.92 521.46 572.66 502.07 459.52 535.86 743.15 487.93
Intensity Change Log 2 — 0.207020 0.967016 0.119095 — 0.026630 0.258522 -0.003589 — 0.111648 0.624759 0.053620

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001042 0.005640 0.013897 0.006231 0.003747 0.003129 0.003928 0.003606 0.002151 0.004072 0.006166 0.005914
Bud Neck 0.000220 0.001897 0.006559 0.001814 0.001287 0.000860 0.001143 0.002003 0.000658 0.001249 0.002358 0.001836
Bud Site 0.000123 0.001089 0.002535 0.003596 0.000802 0.000548 0.000744 0.001708 0.000401 0.000752 0.001146 0.003368
Cell Periphery 0.000069 0.000092 0.000235 0.000598 0.000089 0.000058 0.000057 0.000216 0.000077 0.000071 0.000097 0.000551
Cytoplasm 0.000093 0.000272 0.000214 0.000410 0.000239 0.000212 0.000220 0.000414 0.000153 0.000234 0.000218 0.000411
Cytoplasmic Foci 0.012794 0.025111 0.034627 0.009153 0.026268 0.018158 0.014322 0.011945 0.018319 0.020769 0.018880 0.009491
Eisosomes 0.001323 0.002283 0.006136 0.001269 0.002176 0.001037 0.001080 0.001018 0.001673 0.001505 0.002215 0.001239
Endoplasmic Reticulum 0.000064 0.000101 0.000181 0.000273 0.000151 0.000079 0.000053 0.000191 0.000100 0.000087 0.000082 0.000263
Endosome 0.000476 0.001398 0.000598 0.003651 0.000717 0.001135 0.000701 0.004174 0.000575 0.001234 0.000678 0.003715
Golgi 0.010961 0.019896 0.009740 0.026128 0.013265 0.016472 0.013077 0.031158 0.011906 0.017758 0.012328 0.026736
Lipid Particles 0.006711 0.006851 0.008771 0.004598 0.006739 0.006270 0.004998 0.002822 0.006722 0.006488 0.005845 0.004383
Mitochondria 0.944456* 0.903266* 0.851883* 0.907196* 0.910464* 0.932272* 0.933176* 0.901079* 0.930518* 0.921377* 0.914928* 0.906457*
Mitotic Spindle 0.000248 0.000702 0.008759 0.001205 0.000594 0.000463 0.003030 0.001967 0.000390 0.000553 0.004316 0.001297
None 0.006610 0.003630 0.009097 0.002155 0.004576 0.002524 0.003277 0.003253 0.005776 0.002939 0.004583 0.002288
Nuclear Periphery 0.000613 0.000272 0.001863 0.000529 0.000598 0.000251 0.000726 0.000477 0.000606 0.000259 0.000981 0.000523
Nuclear Periphery Foci 0.000203 0.000254 0.000587 0.000221 0.000415 0.000191 0.000215 0.000209 0.000290 0.000215 0.000298 0.000219
Nucleolus 0.000428 0.002153 0.003426 0.000445 0.001030 0.000770 0.000877 0.000470 0.000675 0.001289 0.001449 0.000448
Nucleus 0.000037 0.000568 0.000233 0.000078 0.000092 0.000118 0.000059 0.000060 0.000060 0.000287 0.000098 0.000076
Peroxisomes 0.003111 0.015571 0.015813 0.009166 0.013592 0.006574 0.007177 0.014625 0.007408 0.009954 0.009116 0.009826
Vacuole 0.003089 0.003816 0.008770 0.008370 0.005690 0.004509 0.003520 0.006535 0.004156 0.004249 0.004698 0.008148
Vacuole Periphery 0.007332 0.005136 0.016077 0.012915 0.007468 0.004373 0.007620 0.012071 0.007388 0.004659 0.009519 0.012813

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.12 -5.19 -4.78 -0.44 2.84 0.66 -0.19 0.14 -0.59 0.39 -3.35 -5.13 -3.74 -1.75 0.20
Bud Neck -4.85 -6.41 -6.46 0.15 4.63 1.69 0.52 -1.32 -2.22 -1.64 -3.35 -6.32 -4.91 -2.22 1.49
Bud Site -2.84 -5.87 -4.57 -3.05 -1.30 0.67 0.12 -1.45 -2.13 -1.75 -1.70 -3.61 -4.34 -3.82 -3.25
Cell Periphery -0.56 -2.59 -4.00 -3.98 -2.68 1.05 1.20 -2.01 -2.62 -2.70 0.19 -0.72 -4.16 -4.29 -4.05
Cytoplasm -5.25 -4.37 -4.96 -2.03 -3.00 0.15 0.24 -1.21 -1.27 -1.41 -1.65 -1.61 -4.09 -2.31 -2.95
Cytoplasmic Foci -4.97 -5.42 2.57 6.24 6.24 3.30 4.84 4.88 2.38 0.94 -1.55 -0.37 6.38 7.06 5.40
Eisosomes -3.95 -6.66 0.18 3.03 6.42 6.85 5.79 5.68 0.11 0.33 1.25 -2.53 1.74 1.06 3.24
Endoplasmic Reticulum -2.59 -3.95 -3.86 -3.15 -1.53 2.12 2.97 -0.59 -1.93 -2.40 0.79 1.13 -3.29 -3.66 -3.77
Endosome -3.42 -0.71 -5.17 -3.50 -5.02 -1.84 0.08 -2.44 -2.14 -2.45 -3.76 -0.64 -5.60 -4.33 -5.34
Golgi -3.83 0.50 -5.35 -1.84 -4.72 -1.59 0.10 -2.66 -2.18 -2.69 -4.01 -0.30 -5.70 -3.27 -5.29
Lipid Particles -0.11 -1.05 1.66 1.51 2.01 0.39 1.69 2.32 1.97 1.33 0.26 1.02 2.19 1.83 1.30
Mitochondria 6.03 8.40 5.88 -0.43 -4.56 -3.38 -3.47 0.65 2.17 2.22 2.12 3.23 4.12 2.41 1.30
Mitotic Spindle -1.73 -2.81 -2.83 -1.28 2.47 0.72 -2.96 -2.30 -2.55 1.07 -1.17 -4.22 -2.97 -2.38 3.08
None 8.05 -2.86 14.18 4.87 8.24 7.47 3.22 1.81 -1.05 0.03 12.64 3.26 13.86 3.18 6.48
Nuclear Periphery 1.08 -3.03 0.25 -2.19 4.57 1.92 -0.64 0.49 -1.30 1.28 1.74 -1.71 0.38 -2.55 3.32
Nuclear Periphery Foci -0.67 -2.26 -0.26 0.52 2.22 2.08 1.85 1.81 -0.28 0.10 1.21 -0.11 1.08 -0.09 1.36
Nucleolus -1.70 -4.65 -0.18 1.68 4.62 1.17 0.58 2.64 1.59 1.81 -1.55 -3.52 2.05 2.12 4.62
Nucleus -1.12 -3.79 -2.07 1.03 2.77 -0.61 1.31 1.22 1.30 0.01 -1.28 -2.28 -0.89 1.17 0.94
Peroxisomes -6.08 -4.27 -3.77 2.55 2.01 3.66 3.20 -0.16 -1.31 -1.20 -2.11 -1.33 -1.42 0.07 -0.37
Vacuole -0.97 -2.64 -4.08 -3.47 0.13 1.00 2.03 -0.29 -0.65 -0.98 -0.21 -0.69 -3.33 -3.22 -2.70
Vacuole Periphery 1.96 -4.15 -3.49 -4.72 1.30 3.20 -0.13 -1.09 -1.86 -1.06 3.93 -2.22 -3.72 -5.82 -2.12
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Arg5,6

Arg5,6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arg5,6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available