Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of a complex that associates with actin filaments; forms a complex with Aim21p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; TcTex1-type dynein light chain family member; null mutant is sensitive to expression of the top1-T722A allele |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 21 | 7 | 6 | 6 | 22 | 8 | – | 29 | 17 | 27 | 26 | 22 | 12 | 17 | 10 | 6 | 1 | 1 | 36 | 15 | 14 | 5 | 26 | 24 |
Bud | 10 | 5 | 4 | 12 | 31 | 19 | – | 32 | 25 | 60 | 35 | 52 | 5 | 7 | 9 | 5 | 1 | 1 | 2 | 3 | 1 | 1 | 4 | 10 |
Bud Neck | 0 | 0 | 2 | 0 | 2 | 0 | – | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 1 | 4 | 0 | 5 | 7 |
Bud Site | 1 | 0 | 1 | 2 | 10 | 9 | – | 14 | 16 | 8 | 14 | 27 | 2 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 3 | 1 | 2 | – | 7 | 4 | 8 | 10 | 10 | 1 | 2 | 8 | 8 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 229 | 137 | 43 | 211 | 268 | 292 | – | 354 | 250 | 287 | 213 | 267 | 219 | 314 | 305 | 159 | 123 | 126 | 217 | 123 | 47 | 29 | 165 | 225 |
Endoplasmic Reticulum | 5 | 3 | 1 | 4 | 7 | 8 | – | 6 | 12 | 4 | 14 | 8 | 7 | 3 | 1 | 16 | 8 | 12 | 2 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1 | 0 | 2 | 15 | 2 | – | 3 | 3 | 4 | 1 | 3 | 0 | 1 | 3 | 1 | 0 | 4 | 9 | 3 | 3 | 1 | 6 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 11 | 1 | 9 | 38 | 55 | 153 | – | 273 | 317 | 340 | 290 | 419 | 3 | 0 | 3 | 3 | 1 | 7 | 3 | 4 | 8 | 1 | 1 | 7 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 4 | 3 | 3 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 5 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | – | 5 | 2 | 4 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 6 | 17 | 13 | – | 19 | 25 | 29 | 12 | 36 | 0 | 7 | 18 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 1 | 7 | 27 | 3 | – | 21 | 9 | 18 | 7 | 20 | 1 | 2 | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 3 | 2 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 5 | 6 | – | 14 | 11 | 14 | 3 | 7 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 2 | 2 |
Unique Cell Count | 265 | 147 | 54 | 252 | 375 | 411 | 598 | 525 | 598 | 475 | 639 | 240 | 340 | 336 | 168 | 126 | 140 | 284 | 156 | 85 | 46 | 222 | 296 | |
Labelled Cell Count | 278 | 155 | 69 | 291 | 462 | 516 | 783 | 700 | 807 | 633 | 878 | 251 | 355 | 360 | 200 | 139 | 164 | 284 | 156 | 85 | 46 | 222 | 296 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 5.2 | 3.3 | 4.0 | 4.3 | 3.7 | – | 3.8 | 3.3 | 3.5 | 3.5 | 3.4 | 5.3 | 5.7 | 5.6 | 5.7 | 6.0 | 6.2 | 4.7 | 5.2 | 5.5 |
Std Deviation (1e-4) | 0.7 | 1.0 | 0.6 | 1.4 | 1.3 | 1.3 | – | 1.1 | 1.0 | 1.0 | 1.0 | 1.0 | 1.1 | 1.3 | 1.4 | 1.6 | 2.0 | 2.5 | 0.7 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.28 | 0.39 | 0.18 | – | 0.21 | 0.01 | 0.11 | 0.09 | 0.04 | 0.7 | 0.8 | 0.77 | 0.79 | 0.87 | 0.92 | 0.52 | 0.66 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | -1.3 | -1.3 | – | -2.9 | -4.5 | -4.5 | -4.8 | -5.4 | 2.5 | 3.0 | 2.5 | 3.4 | 4.1 | 1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6972 | 1.1422 | 1.163 | 0.6589 | 1.2169 | 0.8999 | 0.6164 | 0.6466 | 0.5943 | 0.3431 | 0.4699 | 0.6104 | 2.2844 | 2.6817 | 2.6801 | 2.6429 | 2.5098 | 2.5605 |
Actin | 0.4828 | 0.2987 | 0.4337 | 0.4815 | 0.3976 | 0.4533 | 0.4306 | 0.3984 | 0.552 | 0.4622 | 0.4717 | 0.4664 | 0.4604 | 0.3764 | 0.5308 | 0.4703 | 0.5311 | 0.4721 |
Bud | 0.0015 | 0.0032 | 0.0137 | 0.0276 | 0.0012 | 0.002 | 0.0017 | 0.0048 | 0.0195 | 0.027 | 0.0035 | 0.0022 | 0.0013 | 0.0049 | 0.0143 | 0.0171 | 0.0061 | 0.0007 |
Bud Neck | 0.0189 | 0.0146 | 0.0161 | 0.0052 | 0.001 | 0.2302 | 0.0093 | 0.0046 | 0.0211 | 0.0024 | 0.1248 | 0.1535 | 0.0239 | 0.0074 | 0.0186 | 0.0048 | 0.0026 | 0.2202 |
Bud Periphery | 0.0022 | 0.0066 | 0.0264 | 0.0743 | 0.0018 | 0.0053 | 0.0033 | 0.0061 | 0.0368 | 0.0659 | 0.0049 | 0.0043 | 0.0021 | 0.0064 | 0.0335 | 0.061 | 0.0148 | 0.0009 |
Bud Site | 0.041 | 0.1962 | 0.0676 | 0.0095 | 0.0058 | 0.0024 | 0.0233 | 0.1305 | 0.0386 | 0.0046 | 0.0041 | 0.0045 | 0.0796 | 0.1851 | 0.0504 | 0.0055 | 0.003 | 0.0016 |
Cell Periphery | 0.002 | 0.0038 | 0.002 | 0.0006 | 0.0002 | 0.0006 | 0.0008 | 0.0015 | 0.0015 | 0.0011 | 0.0005 | 0.0006 | 0.0046 | 0.0046 | 0.0017 | 0.0023 | 0.0004 | 0.0005 |
Cytoplasm | 0.1073 | 0.2303 | 0.1372 | 0.0055 | 0.0878 | 0.106 | 0.0808 | 0.257 | 0.1181 | 0.1524 | 0.0284 | 0.0836 | 0.1128 | 0.2349 | 0.1467 | 0.1037 | 0.0657 | 0.1024 |
Cytoplasmic Foci | 0.1374 | 0.0661 | 0.086 | 0.1591 | 0.1312 | 0.0782 | 0.154 | 0.0467 | 0.0524 | 0.0808 | 0.153 | 0.1105 | 0.0936 | 0.0676 | 0.0358 | 0.0833 | 0.0903 | 0.0818 |
Eisosomes | 0.0017 | 0.0013 | 0.0012 | 0.0008 | 0.0006 | 0.0005 | 0.0008 | 0.0006 | 0.001 | 0.0004 | 0.0005 | 0.0006 | 0.0019 | 0.0016 | 0.0007 | 0.0014 | 0.0007 | 0.0005 |
Endoplasmic Reticulum | 0.0017 | 0.0019 | 0.0031 | 0.0002 | 0.0011 | 0.003 | 0.0037 | 0.0022 | 0.0024 | 0.0049 | 0.001 | 0.0047 | 0.0035 | 0.0017 | 0.003 | 0.0019 | 0.0017 | 0.001 |
Endosome | 0.0189 | 0.0036 | 0.0175 | 0.0272 | 0.0057 | 0.0072 | 0.0514 | 0.008 | 0.0164 | 0.0318 | 0.0412 | 0.0232 | 0.038 | 0.0054 | 0.0087 | 0.016 | 0.0191 | 0.009 |
Golgi | 0.0213 | 0.0035 | 0.0104 | 0.0194 | 0.0093 | 0.0102 | 0.0273 | 0.0104 | 0.0175 | 0.0167 | 0.0516 | 0.0226 | 0.0182 | 0.0097 | 0.0108 | 0.0253 | 0.0284 | 0.0083 |
Lipid Particles | 0.0113 | 0.004 | 0.0131 | 0.0382 | 0.0104 | 0.0086 | 0.0284 | 0.0009 | 0.0029 | 0.0019 | 0.029 | 0.0105 | 0.0131 | 0.0014 | 0.005 | 0.0167 | 0.0244 | 0.0033 |
Mitochondria | 0.0039 | 0.001 | 0.0126 | 0.0054 | 0.0711 | 0.006 | 0.012 | 0.003 | 0.0166 | 0.0081 | 0.0069 | 0.0091 | 0.0056 | 0.001 | 0.0107 | 0.0176 | 0.0146 | 0.0017 |
None | 0.0827 | 0.1442 | 0.1177 | 0.0063 | 0.151 | 0.0607 | 0.0541 | 0.0814 | 0.0617 | 0.1076 | 0.0084 | 0.0516 | 0.0575 | 0.0649 | 0.0696 | 0.0324 | 0.0801 | 0.0491 |
Nuclear Periphery | 0.0019 | 0.0003 | 0.0013 | 0.0001 | 0.0004 | 0.0029 | 0.0041 | 0.0004 | 0.0004 | 0.0011 | 0.0006 | 0.0047 | 0.0092 | 0.0003 | 0.0006 | 0.0007 | 0.001 | 0.0015 |
Nucleolus | 0.0011 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0025 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0021 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Nucleus | 0.0014 | 0.0005 | 0.0008 | 0.0001 | 0.0003 | 0.001 | 0.0049 | 0.0006 | 0.0005 | 0.0008 | 0.0002 | 0.0011 | 0.0028 | 0.0005 | 0.0006 | 0.0005 | 0.0003 | 0.0003 |
Peroxisomes | 0.0547 | 0.0179 | 0.0347 | 0.1337 | 0.1205 | 0.0179 | 0.0923 | 0.0412 | 0.0381 | 0.0245 | 0.0668 | 0.0407 | 0.0583 | 0.0244 | 0.0555 | 0.1334 | 0.1124 | 0.0425 |
Punctate Nuclear | 0.005 | 0.001 | 0.0031 | 0.0036 | 0.0018 | 0.0019 | 0.0103 | 0.0009 | 0.0006 | 0.0012 | 0.0017 | 0.003 | 0.0057 | 0.0008 | 0.0008 | 0.0047 | 0.0014 | 0.0021 |
Vacuole | 0.0009 | 0.0008 | 0.0009 | 0.001 | 0.0004 | 0.0006 | 0.0027 | 0.0007 | 0.0011 | 0.0037 | 0.0005 | 0.0012 | 0.0042 | 0.0006 | 0.0014 | 0.0007 | 0.001 | 0.0002 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0009 | 0.0015 | 0.0001 | 0.0004 | 0.001 | 0.0005 | 0.0011 | 0.0012 | 0.0001 | 0.0006 | 0.0005 | 0.0006 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.5127 | 2.5889 | 7.6843 | 21.0751 | 28.1617 | 11.5779 | 16.3603 | 18.5507 | 14.2944 | 21.4018 |
Translational Efficiency | 0.5235 | 9.9531 | 3.2673 | 1.3271 | 0.7496 | 2.2681 | 1.8581 | 1.8168 | 2.2811 | 1.5776 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1802 | 1645 | 1256 | 637 | 1461 | 1439 | 1324 | 1449 | 3263 | 3084 | 2580 | 2086 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 705.60 | 796.10 | 896.01 | 906.72 | 591.37 | 779.27 | 858.36 | 901.20 | 654.45 | 788.25 | 876.69 | 902.89 |
Standard Deviation | 80.76 | 97.10 | 111.16 | 103.36 | 74.72 | 104.70 | 104.21 | 116.90 | 96.58 | 101.06 | 109.28 | 112.97 |
Intensity Change Log 2 | — | 0.174099 | 0.344664 | 0.361807 | — | 0.398062 | 0.537522 | 0.607786 | — | 0.280546 | 0.435808 | 0.479186 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.042177 | 0.141931* | 0.046068 | 0.261548* | 0.079946 | 0.083676 | 0.320331* | 0.294199* | 0.059088 | 0.114749* | 0.186814* | 0.284229* |
Bud Neck | 0.043256 | 0.035927 | 0.034430 | 0.025194 | 0.033864 | 0.051398 | 0.029042 | 0.024295 | 0.039051 | 0.043145 | 0.031665 | 0.024570 |
Bud Site | 0.037228 | 0.079649 | 0.120910* | 0.202337* | 0.026923 | 0.043779 | 0.223928* | 0.185321* | 0.032614 | 0.062912 | 0.173777* | 0.190517* |
Cell Periphery | 0.000896 | 0.001299 | 0.001915 | 0.005101 | 0.001709 | 0.003048 | 0.003573 | 0.006502 | 0.001260 | 0.002115 | 0.002766 | 0.006074 |
Cytoplasm | 0.310588* | 0.199445* | 0.257208* | 0.162734* | 0.105270* | 0.230438* | 0.139745* | 0.206099* | 0.218657* | 0.213906* | 0.196928* | 0.192856* |
Cytoplasmic Foci | 0.307540* | 0.199928* | 0.087943 | 0.053913 | 0.238243* | 0.232721* | 0.050252 | 0.044927 | 0.276512* | 0.215229* | 0.068601 | 0.047671 |
Eisosomes | 0.006329 | 0.005657 | 0.000797 | 0.004789 | 0.006382 | 0.009634 | 0.002814 | 0.007422 | 0.006353 | 0.007513 | 0.001832 | 0.006618 |
Endoplasmic Reticulum | 0.000853 | 0.001439 | 0.012845 | 0.010018 | 0.000490 | 0.000942 | 0.006170 | 0.004594 | 0.000691 | 0.001207 | 0.009420 | 0.006251 |
Endosome | 0.010142 | 0.013502 | 0.007187 | 0.003755 | 0.017358 | 0.016117 | 0.003484 | 0.005469 | 0.013373 | 0.014722 | 0.005287 | 0.004946 |
Golgi | 0.017837 | 0.028340 | 0.001612 | 0.001101 | 0.051640 | 0.030416 | 0.001484 | 0.004782 | 0.032972 | 0.029309 | 0.001546 | 0.003658 |
Lipid Particles | 0.013744 | 0.008252 | 0.011305 | 0.004776 | 0.044658 | 0.015698 | 0.004507 | 0.002804 | 0.027586 | 0.011726 | 0.007816 | 0.003406 |
Mitochondria | 0.011248 | 0.017286 | 0.001682 | 0.000918 | 0.033108 | 0.025915 | 0.001602 | 0.007013 | 0.021036 | 0.021312 | 0.001641 | 0.005152 |
Mitotic Spindle | 0.000527 | 0.005713 | 0.006658 | 0.006298 | 0.001258 | 0.005599 | 0.003151 | 0.004502 | 0.000854 | 0.005660 | 0.004858 | 0.005051 |
None | 0.001015 | 0.000659 | 0.003734 | 0.001735 | 0.001226 | 0.001023 | 0.000281 | 0.000262 | 0.001109 | 0.000829 | 0.001962 | 0.000712 |
Nuclear Periphery | 0.000129 | 0.000088 | 0.001070 | 0.000166 | 0.000062 | 0.000080 | 0.000097 | 0.000109 | 0.000099 | 0.000084 | 0.000571 | 0.000127 |
Nuclear Periphery Foci | 0.000165 | 0.000429 | 0.002680 | 0.001924 | 0.000210 | 0.000175 | 0.001019 | 0.000588 | 0.000185 | 0.000310 | 0.001828 | 0.000996 |
Nucleolus | 0.000284 | 0.000170 | 0.000764 | 0.000077 | 0.000487 | 0.000757 | 0.000062 | 0.000117 | 0.000375 | 0.000444 | 0.000404 | 0.000105 |
Nucleus | 0.058678 | 0.020939 | 0.264147* | 0.056656 | 0.018940 | 0.027450 | 0.041469 | 0.044685 | 0.040885 | 0.023977 | 0.149873* | 0.048340 |
Peroxisomes | 0.113301* | 0.215659* | 0.057049 | 0.069149 | 0.332219* | 0.195679* | 0.114379* | 0.056223 | 0.211321* | 0.206336* | 0.086470 | 0.060170 |
Vacuole | 0.023378 | 0.022932 | 0.079693 | 0.127657 | 0.005439 | 0.024066 | 0.052508 | 0.098800 | 0.015346 | 0.023461 | 0.065743 | 0.107612 |
Vacuole Periphery | 0.000686 | 0.000755 | 0.000305 | 0.000154 | 0.000568 | 0.001389 | 0.000101 | 0.001287 | 0.000633 | 0.001051 | 0.000200 | 0.000941 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -15.74 | 0.55 | -15.21 | -5.97 | -15.29 | -0.55 | -21.92 | -19.42 | -18.76 | 2.75 | -12.09 | -18.82 | -26.57 | -17.40 | -9.15 |
Bud Neck | 2.51 | 3.69 | 7.18 | 4.70 | 3.51 | -4.46 | 1.79 | 3.67 | 9.04 | 2.66 | -1.64 | 4.14 | 7.95 | 9.81 | 4.43 |
Bud Site | -10.11 | -12.13 | -15.87 | -10.60 | -6.80 | -4.86 | -26.65* | -22.50 | -19.60 | 4.91 | -10.89 | -27.58 | -27.32 | -20.39 | -1.54 |
Cell Periphery | -3.14 | -8.07 | -9.05 | -7.73 | -6.61 | -2.88 | -5.36 | -7.04 | -4.14 | -4.19 | -3.77 | -8.35 | -10.21 | -7.48 | -6.80 |
Cytoplasm | 13.79 | 7.51 | 14.88 | 3.49 | 7.29 | -16.58 | -6.54 | -14.26 | 2.28 | -7.39 | 0.70 | 3.76 | 3.31 | 2.67 | -0.30 |
Cytoplasmic Foci | 14.68 | 34.21 | 38.66 | 23.10 | 5.40 | 0.80 | 30.71 | 32.05 | 32.01 | 1.75 | 11.45 | 46.45 | 52.25 | 39.99 | 6.26 |
Eisosomes | 1.11 | 11.35 | 3.14 | 2.37 | -5.56 | -3.78 | 6.86 | -0.18 | 3.16 | -5.46 | -2.16 | 12.60 | 0.96 | 2.70 | -7.74 |
Endoplasmic Reticulum | -2.26 | -13.43 | -8.36 | -7.70 | 2.89 | -3.13 | -10.98 | -10.49 | -9.24 | 2.96 | -3.37 | -17.07 | -12.95 | -11.43 | 5.61 |
Endosome | -3.22 | 4.12 | 6.78 | 8.47 | 3.87 | 0.74 | 11.09 | 8.69 | 8.07 | -1.36 | -1.46 | 11.71 | 10.18 | 10.96 | 1.23 |
Golgi | -5.38 | 15.64 | 16.38 | 16.71 | 1.58 | 6.88 | 19.31 | 17.11 | 13.45 | -2.63 | 2.15 | 23.67 | 19.97 | 19.18 | -2.40 |
Lipid Particles | 4.83 | 1.54 | 8.46 | 3.65 | 6.88 | 9.33 | 13.74 | 14.62 | 9.93 | 3.44 | 10.19 | 13.03 | 16.97 | 11.00 | 8.55 |
Mitochondria | -3.04 | 8.73 | 9.47 | 10.07 | 3.95 | 2.28 | 13.49 | 10.22 | 7.70 | -3.10 | -0.09 | 15.81 | 10.92 | 10.27 | -2.87 |
Mitotic Spindle | -4.54 | -5.52 | -2.75 | 0.01 | 0.91 | -3.05 | -2.42 | -2.29 | 0.98 | -0.56 | -5.35 | -6.08 | -3.80 | 0.93 | 0.83 |
None | 1.61 | -3.69 | -0.38 | -0.69 | 1.63 | 0.38 | 2.46 | 2.58 | 1.63 | 1.08 | 0.93 | -1.89 | 1.39 | 0.59 | 2.69 |
Nuclear Periphery | 0.70 | -16.30 | -2.93 | -3.74 | 15.78 | -1.10 | -5.93 | -5.12 | -2.94 | 0.41 | 0.37 | -15.80 | -3.38 | -3.79 | 15.37 |
Nuclear Periphery Foci | -1.13 | -13.51 | -5.85 | -4.19 | 2.30 | 0.20 | -5.38 | -4.38 | -7.24 | 3.13 | -0.99 | -13.41 | -7.99 | -4.98 | 5.61 |
Nucleolus | 3.08 | -4.83 | 7.19 | 3.54 | 6.67 | -0.65 | 3.16 | 2.89 | 1.64 | -0.47 | -0.40 | -0.74 | 4.19 | 1.86 | 5.60 |
Nucleus | 14.31 | -26.59 | -1.41 | -9.40 | 23.30 | -3.53 | -9.88 | -10.60 | -8.45 | 0.39 | 9.13 | -24.69 | -5.82 | -13.08 | 20.42 |
Peroxisomes | -14.01 | 11.71 | 8.46 | 20.39 | -1.56 | 14.06 | 25.55 | 35.06* | 21.21 | 9.59 | 0.89 | 24.58 | 30.71 | 30.84 | 6.26 |
Vacuole | -0.63 | -19.26 | -18.01 | -17.72 | -8.92 | -10.59 | -17.95 | -25.08 | -20.89 | -10.26 | -6.85 | -25.68 | -29.73 | -27.31 | -12.76 |
Vacuole Periphery | -0.47 | 3.13 | 4.55 | 6.03 | 1.94 | -2.76 | 4.13 | -1.40 | 0.69 | -2.80 | -2.64 | 5.19 | -0.63 | 0.91 | -2.44 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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