Standard name | |
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Human Ortholog | |
Description | Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 4 | 0 | 3 | 1 | 1 | 4 | 4 | – | 6 | 9 | 7 | 6 | 7 | 3 | 1 | 3 | 0 | 1 | 3 | 0 | 1 | 1 | 2 |
Bud | 0 | 2 | 0 | 1 | 1 | 8 | 9 | 9 | – | 9 | 15 | 17 | 5 | 3 | 1 | 0 | 0 | 0 | 4 | 5 | 0 | 5 | 5 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 0 | 11 | 6 | 19 | 23 | 23 | – | 35 | 48 | 28 | 13 | 34 | 22 | 0 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 241 | 206 | 41 | 101 | 119 | 181 | 252 | 252 | – | 278 | 193 | 197 | 319 | 427 | 524 | 76 | 72 | 65 | 14 | 13 | 0 | 7 | 6 | 10 |
Endoplasmic Reticulum | 73 | 78 | 24 | 19 | 29 | 30 | 37 | 30 | – | 28 | 6 | 12 | 176 | 157 | 88 | 4 | 4 | 4 | 226 | 216 | 44 | 159 | 244 | 305 |
Endosome | 15 | 13 | 0 | 3 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 2 | 5 | 7 | 6 | 9 | 6 | 3 | 1 | 0 | 4 | 2 |
Golgi | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 6 | 1 | 0 | 1 | 3 | 2 |
Mitochondria | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 1 | – | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 2 | 4 |
Nucleus | 6 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | – | 0 | 0 | 1 | 1 | 1 | 0 | 15 | 13 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 242 | 82 | 19 | 6 | 7 | 3 | 9 | 11 | – | 7 | 2 | 7 | 34 | 26 | 41 | 119 | 131 | 121 | 347 | 175 | 70 | 152 | 144 | 152 |
Unique Cell Count | 527 | 356 | 81 | 125 | 154 | 218 | 299 | 295 | 314 | 219 | 223 | 490 | 585 | 631 | 199 | 206 | 177 | 619 | 426 | 120 | 332 | 418 | 496 | |
Labelled Cell Count | 584 | 388 | 89 | 145 | 164 | 244 | 338 | 332 | 365 | 274 | 271 | 554 | 658 | 685 | 226 | 237 | 209 | 619 | 426 | 120 | 332 | 418 | 496 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.1 | 14.0 | 8.6 | 11.6 | 12.2 | 10.7 | 10.0 | 10.0 | – | 10.2 | 10.8 | 10.6 | 13.4 | 12.7 | 10.9 | 12.8 | 13.9 | 14.8 | 15.6 | 16.7 | 16.9 |
Std Deviation (1e-4) | 5.7 | 8.5 | 2.0 | 4.5 | 4.5 | 2.0 | 2.1 | 2.1 | – | 2.6 | 2.5 | 2.5 | 4.6 | 5.0 | 4.3 | 7.1 | 8.7 | 10.6 | 6.4 | 7.3 | 6.6 |
Intensity Change (Log2) | – | – | – | 0.44 | 0.51 | 0.32 | 0.22 | 0.22 | – | 0.25 | 0.33 | 0.31 | 0.65 | 0.56 | 0.35 | 0.57 | 0.7 | 0.78 | 0.86 | 0.96 | 0.98 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 2.7 | 0 | 0 | – | 3.1 | 4.6 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.6 | 4.2 | 5.7 | 6.4 | 6.7 | – | 7.7 | 7.0 | 7.1 | 2.5 | 4.1 | 6.8 | -1.9 | -2.4 | -2.1 |
Endoplasmic Reticulum | -2.5 | -1.9 | -3.2 | -3.8 | -4.4 | – | -4.9 | -6.9 | -5.8 | 1.1 | -0.5 | -3.6 | -7.0 | -7.1 | -6.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.0 | -4.4 | -6.5 | -6.2 | -5.8 | – | -6.9 | -6.8 | -5.6 | -4.7 | -6.4 | -5.2 | 5.5 | 6.1 | 6.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.0731 | 9.503 | 9.4207 | 9.143 | 8.7795 | 9.3297 | 9.0594 | 8.9961 | 12.5729 | 8.5231 | 12.3186 | 9.0239 | 9.9645 | 12.4674 | 10.7611 | 10.918 | 10.2616 | 10.3451 |
Actin | 0.0002 | 0.0005 | 0.0008 | 0.0003 | 0.0001 | 0.0001 | 0.0015 | 0.0012 | 0.0055 | 0.0003 | 0.0009 | 0.0012 | 0.0096 | 0.001 | 0.0051 | 0.0081 | 0.0001 | 0.0073 |
Bud | 0.0008 | 0.0004 | 0.0027 | 0.0099 | 0.0004 | 0.0008 | 0.001 | 0.0162 | 0.0044 | 0.017 | 0.0036 | 0.0058 | 0.0004 | 0.0003 | 0.0007 | 0.0003 | 0.0003 | 0.0007 |
Bud Neck | 0.0006 | 0.0017 | 0.0015 | 0.0011 | 0.0049 | 0.0027 | 0.0005 | 0.0018 | 0.0023 | 0.0019 | 0.002 | 0.0021 | 0.0015 | 0.0018 | 0.0011 | 0.0154 | 0.0034 | 0.0051 |
Bud Periphery | 0.0009 | 0.0009 | 0.0021 | 0.0047 | 0.0015 | 0.0012 | 0.0035 | 0.0177 | 0.0122 | 0.0353 | 0.0243 | 0.0262 | 0.0008 | 0.0009 | 0.0016 | 0.0013 | 0.0019 | 0.0029 |
Bud Site | 0.0033 | 0.0052 | 0.0118 | 0.0023 | 0.001 | 0.0005 | 0.0017 | 0.0184 | 0.0028 | 0.002 | 0.0008 | 0.0012 | 0.0018 | 0.0038 | 0.003 | 0.0016 | 0.0002 | 0.0005 |
Cell Periphery | 0.0181 | 0.0152 | 0.0133 | 0.0297 | 0.0226 | 0.0116 | 0.0208 | 0.05 | 0.0374 | 0.0324 | 0.0209 | 0.0151 | 0.021 | 0.0418 | 0.0544 | 0.0289 | 0.0338 | 0.0258 |
Cytoplasm | 0.1481 | 0.2049 | 0.1327 | 0.1797 | 0.1583 | 0.1207 | 0.0837 | 0.096 | 0.0855 | 0.0418 | 0.0782 | 0.0754 | 0.1473 | 0.1452 | 0.0663 | 0.0233 | 0.0276 | 0.0873 |
Cytoplasmic Foci | 0.0055 | 0.0084 | 0.0098 | 0.005 | 0.0051 | 0.0075 | 0.0057 | 0.0026 | 0.0121 | 0.004 | 0.0017 | 0.0035 | 0.0152 | 0.0041 | 0.0063 | 0.0075 | 0.0006 | 0.0056 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.5326 | 0.3732 | 0.4662 | 0.4257 | 0.285 | 0.4599 | 0.3308 | 0.2886 | 0.422 | 0.3467 | 0.3586 | 0.3775 | 0.4348 | 0.4502 | 0.482 | 0.4703 | 0.5191 | 0.4236 |
Endosome | 0.0392 | 0.0536 | 0.054 | 0.0485 | 0.0434 | 0.0489 | 0.0313 | 0.0074 | 0.0316 | 0.0522 | 0.029 | 0.0222 | 0.0569 | 0.0183 | 0.0257 | 0.04 | 0.0122 | 0.022 |
Golgi | 0.0014 | 0.002 | 0.0027 | 0.0017 | 0.0014 | 0.0013 | 0.0016 | 0.0007 | 0.005 | 0.0038 | 0.0016 | 0.0009 | 0.0084 | 0.0016 | 0.0016 | 0.0102 | 0.0003 | 0.0028 |
Lipid Particles | 0.0179 | 0.0134 | 0.0133 | 0.0126 | 0.0209 | 0.0146 | 0.0271 | 0.0164 | 0.0257 | 0.0739 | 0.0049 | 0.0112 | 0.0496 | 0.0222 | 0.0225 | 0.0581 | 0.0133 | 0.017 |
Mitochondria | 0.0019 | 0.0006 | 0.0015 | 0.0024 | 0.0005 | 0.0008 | 0.0006 | 0.0006 | 0.0014 | 0.0009 | 0.004 | 0.0012 | 0.0064 | 0.0016 | 0.0011 | 0.0013 | 0.0001 | 0.0013 |
None | 0.0019 | 0.0016 | 0.002 | 0.0008 | 0.0004 | 0.0006 | 0.3397 | 0.276 | 0.1212 | 0.1411 | 0.0976 | 0.2215 | 0.0083 | 0.0005 | 0.0008 | 0.0005 | 0.0001 | 0.0004 |
Nuclear Periphery | 0.0183 | 0.0201 | 0.0169 | 0.0185 | 0.0198 | 0.0203 | 0.0201 | 0.0118 | 0.0231 | 0.0123 | 0.039 | 0.0241 | 0.0508 | 0.0169 | 0.0161 | 0.0212 | 0.0242 | 0.0345 |
Nucleolus | 0.0014 | 0.0007 | 0.0023 | 0.0006 | 0.0033 | 0.0003 | 0.0005 | 0.0011 | 0.0012 | 0.0005 | 0.0005 | 0.0014 | 0.0007 | 0.0004 | 0.0006 | 0.0008 | 0.0001 | 0.0003 |
Nucleus | 0.0058 | 0.0106 | 0.0085 | 0.0047 | 0.0141 | 0.0065 | 0.0047 | 0.0113 | 0.0098 | 0.0023 | 0.0081 | 0.0206 | 0.0162 | 0.003 | 0.0146 | 0.0022 | 0.0025 | 0.0079 |
Peroxisomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0073 | 0.0001 | 0.0001 | 0.0002 | 0.0086 | 0.001 | 0.0051 | 0.0014 | 0 | 0.0131 |
Punctate Nuclear | 0.0008 | 0.0009 | 0.0019 | 0.0002 | 0.0005 | 0.0029 | 0.0042 | 0.0008 | 0.0063 | 0.0004 | 0.0003 | 0.0023 | 0.0063 | 0.0003 | 0.0006 | 0.002 | 0.0001 | 0.0013 |
Vacuole | 0.1972 | 0.2803 | 0.2495 | 0.2432 | 0.4075 | 0.2922 | 0.1176 | 0.1787 | 0.1777 | 0.2271 | 0.3033 | 0.1788 | 0.1506 | 0.2817 | 0.2869 | 0.2996 | 0.3512 | 0.3342 |
Vacuole Periphery | 0.0038 | 0.0054 | 0.0063 | 0.0081 | 0.0093 | 0.0066 | 0.0029 | 0.0021 | 0.0055 | 0.0039 | 0.0204 | 0.0074 | 0.0046 | 0.0034 | 0.0039 | 0.0059 | 0.0087 | 0.0064 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 335.2652 | 343.9671 | 527.3676 | 647.2756 | 557.5719 | 330.8381 | 523.5951 | 635.1552 | 710.391 | 718.089 |
Translational Efficiency | 2.6034 | 3.0475 | 2.2775 | 1.8493 | 2.1296 | 3.2678 | 2.5632 | 1.8473 | 2.1156 | 2.0709 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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