Standard name
Human Ortholog
Description Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.09 0 0.09 0.08 0.08 0.11 0.22 0.13 0 0.06 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.46 0.58 0.51 0.81 0.77 0.83 0.84 0.85 0.89 0.88 0.88 0.65 0.73 0.83 0.38 0.35 0.37 0 0 0 0 0 0
Endoplasmic Reticulum 0.14 0.22 0.3 0.15 0.19 0.14 0.12 0.1 0.09 0 0.05 0.36 0.27 0.14 0 0 0 0.37 0.51 0.37 0.48 0.58 0.62
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.46 0.23 0.23 0.05 0.05 0 0 0 0 0 0 0.07 0 0.06 0.6 0.64 0.68 0.56 0.41 0.59 0.46 0.35 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 4 0 3 1 1 4 4 6 9 7 6 7 3 1 3 0 1 3 0 1 1 2
Bud 0 2 0 1 1 8 9 9 9 15 17 5 3 1 0 0 0 4 5 0 5 5 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 3 0 11 6 19 23 23 35 48 28 13 34 22 0 6 3 0 0 0 0 0 0
Cytoplasm 241 206 41 101 119 181 252 252 278 193 197 319 427 524 76 72 65 14 13 0 7 6 10
Endoplasmic Reticulum 73 78 24 19 29 30 37 30 28 6 12 176 157 88 4 4 4 226 216 44 159 244 305
Endosome 15 13 0 3 0 1 0 0 1 0 0 0 2 5 7 6 9 6 3 1 0 4 2
Golgi 4 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 1 6 1 0 1 3 2
Mitochondria 0 0 2 0 1 1 1 1 1 1 1 0 0 1 1 0 0 1 2 0 1 2 4
Nucleus 6 0 2 0 0 0 2 2 0 0 1 1 1 0 15 13 5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 0 0 1 0
Nucleolus 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 242 82 19 6 7 3 9 11 7 2 7 34 26 41 119 131 121 347 175 70 152 144 152
Unique Cell Count 527 356 81 125 154 218 299 295 314 219 223 490 585 631 199 206 177 619 426 120 332 418 496
Labelled Cell Count 584 388 89 145 164 244 338 332 365 274 271 554 658 685 226 237 209 619 426 120 332 418 496


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.1 14.0 8.6 11.6 12.2 10.7 10.0 10.0 10.2 10.8 10.6 13.4 12.7 10.9 12.8 13.9 14.8 15.6 16.7 16.9
Std Deviation (1e-4) 5.7 8.5 2.0 4.5 4.5 2.0 2.1 2.1 2.6 2.5 2.5 4.6 5.0 4.3 7.1 8.7 10.6 6.4 7.3 6.6
Intensity Change (Log2) 0.44 0.51 0.32 0.22 0.22 0.25 0.33 0.31 0.65 0.56 0.35 0.57 0.7 0.78 0.86 0.96 0.98


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.7 0 0 3.1 4.6 3.3 0 0 0 0 0 0
Cytoplasm 4.6 4.2 5.7 6.4 6.7 7.7 7.0 7.1 2.5 4.1 6.8 -1.9 -2.4 -2.1
Endoplasmic Reticulum -2.5 -1.9 -3.2 -3.8 -4.4 -4.9 -6.9 -5.8 1.1 -0.5 -3.6 -7.0 -7.1 -6.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.0 -4.4 -6.5 -6.2 -5.8 -6.9 -6.8 -5.6 -4.7 -6.4 -5.2 5.5 6.1 6.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.0731 9.503 9.4207 9.143 8.7795 9.3297 9.0594 8.9961 12.5729 8.5231 12.3186 9.0239 9.9645 12.4674 10.7611 10.918 10.2616 10.3451
Actin 0.0002 0.0005 0.0008 0.0003 0.0001 0.0001 0.0015 0.0012 0.0055 0.0003 0.0009 0.0012 0.0096 0.001 0.0051 0.0081 0.0001 0.0073
Bud 0.0008 0.0004 0.0027 0.0099 0.0004 0.0008 0.001 0.0162 0.0044 0.017 0.0036 0.0058 0.0004 0.0003 0.0007 0.0003 0.0003 0.0007
Bud Neck 0.0006 0.0017 0.0015 0.0011 0.0049 0.0027 0.0005 0.0018 0.0023 0.0019 0.002 0.0021 0.0015 0.0018 0.0011 0.0154 0.0034 0.0051
Bud Periphery 0.0009 0.0009 0.0021 0.0047 0.0015 0.0012 0.0035 0.0177 0.0122 0.0353 0.0243 0.0262 0.0008 0.0009 0.0016 0.0013 0.0019 0.0029
Bud Site 0.0033 0.0052 0.0118 0.0023 0.001 0.0005 0.0017 0.0184 0.0028 0.002 0.0008 0.0012 0.0018 0.0038 0.003 0.0016 0.0002 0.0005
Cell Periphery 0.0181 0.0152 0.0133 0.0297 0.0226 0.0116 0.0208 0.05 0.0374 0.0324 0.0209 0.0151 0.021 0.0418 0.0544 0.0289 0.0338 0.0258
Cytoplasm 0.1481 0.2049 0.1327 0.1797 0.1583 0.1207 0.0837 0.096 0.0855 0.0418 0.0782 0.0754 0.1473 0.1452 0.0663 0.0233 0.0276 0.0873
Cytoplasmic Foci 0.0055 0.0084 0.0098 0.005 0.0051 0.0075 0.0057 0.0026 0.0121 0.004 0.0017 0.0035 0.0152 0.0041 0.0063 0.0075 0.0006 0.0056
Eisosomes 0 0 0 0 0 0 0.0002 0.0002 0.0001 0 0 0.0001 0.0002 0.0001 0 0.0001 0 0
Endoplasmic Reticulum 0.5326 0.3732 0.4662 0.4257 0.285 0.4599 0.3308 0.2886 0.422 0.3467 0.3586 0.3775 0.4348 0.4502 0.482 0.4703 0.5191 0.4236
Endosome 0.0392 0.0536 0.054 0.0485 0.0434 0.0489 0.0313 0.0074 0.0316 0.0522 0.029 0.0222 0.0569 0.0183 0.0257 0.04 0.0122 0.022
Golgi 0.0014 0.002 0.0027 0.0017 0.0014 0.0013 0.0016 0.0007 0.005 0.0038 0.0016 0.0009 0.0084 0.0016 0.0016 0.0102 0.0003 0.0028
Lipid Particles 0.0179 0.0134 0.0133 0.0126 0.0209 0.0146 0.0271 0.0164 0.0257 0.0739 0.0049 0.0112 0.0496 0.0222 0.0225 0.0581 0.0133 0.017
Mitochondria 0.0019 0.0006 0.0015 0.0024 0.0005 0.0008 0.0006 0.0006 0.0014 0.0009 0.004 0.0012 0.0064 0.0016 0.0011 0.0013 0.0001 0.0013
None 0.0019 0.0016 0.002 0.0008 0.0004 0.0006 0.3397 0.276 0.1212 0.1411 0.0976 0.2215 0.0083 0.0005 0.0008 0.0005 0.0001 0.0004
Nuclear Periphery 0.0183 0.0201 0.0169 0.0185 0.0198 0.0203 0.0201 0.0118 0.0231 0.0123 0.039 0.0241 0.0508 0.0169 0.0161 0.0212 0.0242 0.0345
Nucleolus 0.0014 0.0007 0.0023 0.0006 0.0033 0.0003 0.0005 0.0011 0.0012 0.0005 0.0005 0.0014 0.0007 0.0004 0.0006 0.0008 0.0001 0.0003
Nucleus 0.0058 0.0106 0.0085 0.0047 0.0141 0.0065 0.0047 0.0113 0.0098 0.0023 0.0081 0.0206 0.0162 0.003 0.0146 0.0022 0.0025 0.0079
Peroxisomes 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0073 0.0001 0.0001 0.0002 0.0086 0.001 0.0051 0.0014 0 0.0131
Punctate Nuclear 0.0008 0.0009 0.0019 0.0002 0.0005 0.0029 0.0042 0.0008 0.0063 0.0004 0.0003 0.0023 0.0063 0.0003 0.0006 0.002 0.0001 0.0013
Vacuole 0.1972 0.2803 0.2495 0.2432 0.4075 0.2922 0.1176 0.1787 0.1777 0.2271 0.3033 0.1788 0.1506 0.2817 0.2869 0.2996 0.3512 0.3342
Vacuole Periphery 0.0038 0.0054 0.0063 0.0081 0.0093 0.0066 0.0029 0.0021 0.0055 0.0039 0.0204 0.0074 0.0046 0.0034 0.0039 0.0059 0.0087 0.0064

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 335.2652 343.9671 527.3676 647.2756 557.5719 330.8381 523.5951 635.1552 710.391 718.089
Translational Efficiency 2.6034 3.0475 2.2775 1.8493 2.1296 3.2678 2.5632 1.8473 2.1156 2.0709

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (85%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Vtc1

Vtc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vtc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available