Standard name | |
---|---|
Human Ortholog | |
Description | 3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER3 has a paralog, SER33, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 3 | 0 | 6 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 |
Bud | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 0 | 2 | 0 | 0 | 7 | 6 | 15 | 5 | 4 | 22 | 1 | 1 | 1 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 171 | 357 | 40 | 59 | 56 | 90 | 97 | 111 | 101 | 52 | 51 | 71 | 266 | 258 | 291 | 318 | 131 | 208 | 165 | 341 | 58 | 155 | 186 | 173 |
Endoplasmic Reticulum | 0 | 4 | 0 | 0 | 0 | 1 | 4 | 11 | 7 | 7 | 7 | 11 | 5 | 7 | 3 | 12 | 5 | 4 | 0 | 2 | 2 | 5 | 3 | 17 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 6 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 3 | 4 | 5 | 6 | 2 | 3 | 7 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 3 | 1 | 0 | 3 | 0 | 0 | 1 | 4 |
Unique Cell Count | 172 | 361 | 40 | 60 | 56 | 94 | 111 | 130 | 119 | 63 | 64 | 98 | 272 | 265 | 294 | 329 | 137 | 212 | 169 | 359 | 66 | 163 | 196 | 210 |
Labelled Cell Count | 175 | 365 | 40 | 61 | 56 | 96 | 122 | 138 | 134 | 69 | 73 | 119 | 274 | 266 | 296 | 349 | 148 | 218 | 169 | 359 | 66 | 163 | 196 | 210 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 49.9 | 59.7 | 34.7 | 35.5 | 36.9 | 31.7 | 26.4 | 26.2 | 26.2 | 23.8 | 24.4 | 24.1 | 104.8 | 107.7 | 103.0 | 59.1 | 68.9 | 69.6 | 64.7 | 64.3 | 60.0 |
Std Deviation (1e-4) | 12.5 | 15.4 | 8.6 | 10.5 | 14.8 | 9.5 | 8.3 | 8.0 | 7.5 | 5.8 | 8.3 | 8.1 | 30.8 | 35.3 | 30.8 | 18.0 | 22.8 | 19.5 | 19.2 | 17.9 | 23.7 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.09 | -0.13 | -0.39 | -0.4 | -0.4 | -0.54 | -0.51 | -0.52 | 1.6 | 1.64 | 1.57 | 0.77 | 0.99 | 1.01 | 0.9 | 0.89 | 0.79 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.8 | 0 | -1.3 | -2.4 | -2.6 | -2.6 | -2.8 | -3.0 | -3.7 | -0.9 | -1.0 | -0.6 | -1.2 | -1.3 | -0.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 37.9452 | 54.2367 | 54.6729 | 49.176 | 47.4498 | 48.3256 | 39.1363 | 52.737 | 53.997 | 42.8257 | 43.172 | 49.3185 | 32.2231 | 51.3594 | 46.0623 | 59.2026 | 42.2677 | 47.199 |
Actin | 0.0299 | 0.0008 | 0.0005 | 0.0038 | 0.049 | 0.0005 | 0.0243 | 0.0036 | 0.0127 | 0.0021 | 0.0017 | 0.0011 | 0.0653 | 0.0009 | 0.0049 | 0.0002 | 0.0012 | 0.0026 |
Bud | 0.0019 | 0.0015 | 0.0016 | 0.0012 | 0.0005 | 0.0008 | 0.0014 | 0.0021 | 0.0007 | 0.0152 | 0.0019 | 0.0012 | 0.0023 | 0.0006 | 0.0009 | 0.0008 | 0.0004 | 0.0015 |
Bud Neck | 0.0011 | 0.0005 | 0.0007 | 0.0008 | 0.002 | 0.0018 | 0.0006 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0012 | 0.0013 | 0.0004 | 0.0004 | 0.0006 | 0.0005 | 0.0024 |
Bud Periphery | 0.0029 | 0.0011 | 0.0019 | 0.0011 | 0.0005 | 0.0006 | 0.0016 | 0.0053 | 0.0007 | 0.0359 | 0.0003 | 0.0006 | 0.0016 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0011 |
Bud Site | 0.0016 | 0.0031 | 0.0012 | 0.0008 | 0.0004 | 0.0001 | 0.0034 | 0.0007 | 0.0003 | 0.0019 | 0.0006 | 0.0001 | 0.0014 | 0.0015 | 0.0007 | 0.0001 | 0.0003 | 0.0002 |
Cell Periphery | 0.0067 | 0.0103 | 0.0068 | 0.0027 | 0.0031 | 0.0005 | 0.0026 | 0.0006 | 0.0005 | 0.0006 | 0.0001 | 0.0003 | 0.0027 | 0.0029 | 0.0015 | 0.0007 | 0.0001 | 0.0016 |
Cytoplasm | 0.6204 | 0.919 | 0.8483 | 0.7517 | 0.7696 | 0.883 | 0.7664 | 0.9679 | 0.9392 | 0.8037 | 0.9717 | 0.9019 | 0.6442 | 0.9535 | 0.888 | 0.6402 | 0.7276 | 0.8786 |
Cytoplasmic Foci | 0.0094 | 0.0036 | 0.0027 | 0.0096 | 0.011 | 0.0134 | 0.0241 | 0.002 | 0.0024 | 0.0083 | 0.0083 | 0.0047 | 0.0128 | 0.0092 | 0.0124 | 0.0038 | 0.0293 | 0.0073 |
Eisosomes | 0.0004 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0823 | 0.0132 | 0.0128 | 0.0185 | 0.0082 | 0.0434 | 0.0707 | 0.0033 | 0.0031 | 0.0166 | 0.0004 | 0.0415 | 0.0371 | 0.0052 | 0.0087 | 0.0074 | 0.0121 | 0.0295 |
Endosome | 0.0299 | 0.0033 | 0.005 | 0.0302 | 0.0177 | 0.0108 | 0.0183 | 0.0016 | 0.0169 | 0.0414 | 0.0053 | 0.014 | 0.0163 | 0.0016 | 0.0074 | 0.0382 | 0.0638 | 0.0257 |
Golgi | 0.0089 | 0.0004 | 0.0004 | 0.006 | 0.0017 | 0.001 | 0.0056 | 0.0006 | 0.0016 | 0.0019 | 0.0002 | 0.0024 | 0.0107 | 0.0004 | 0.0041 | 0.0005 | 0.0058 | 0.0031 |
Lipid Particles | 0.006 | 0.0013 | 0.0007 | 0.0464 | 0.0032 | 0.0005 | 0.0093 | 0.0001 | 0.0007 | 0.0016 | 0.0001 | 0.0001 | 0.0325 | 0.0022 | 0.0088 | 0.0002 | 0.0053 | 0.0006 |
Mitochondria | 0.0189 | 0.0005 | 0.0022 | 0.0058 | 0.0451 | 0.0056 | 0.0054 | 0.0005 | 0.0009 | 0.0019 | 0.0001 | 0.0069 | 0.0341 | 0.0004 | 0.0244 | 0.0004 | 0.0023 | 0.0033 |
None | 0.0055 | 0.0006 | 0.0015 | 0.001 | 0.0047 | 0.0028 | 0.0105 | 0.0004 | 0.0009 | 0.0039 | 0.0008 | 0.0006 | 0.003 | 0.0019 | 0.0036 | 0.0002 | 0.0143 | 0.0007 |
Nuclear Periphery | 0.0293 | 0.0022 | 0.0082 | 0.0052 | 0.011 | 0.0044 | 0.0152 | 0.0034 | 0.0025 | 0.0183 | 0.0006 | 0.0023 | 0.016 | 0.002 | 0.0039 | 0.0062 | 0.0668 | 0.01 |
Nucleolus | 0.006 | 0.0004 | 0.0019 | 0.0005 | 0.0138 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0007 | 0 | 0 | 0.0251 | 0.0006 | 0.0006 | 0.0001 | 0.0011 | 0.0001 |
Nucleus | 0.0234 | 0.0025 | 0.0055 | 0.004 | 0.0075 | 0.0025 | 0.0064 | 0.0015 | 0.0011 | 0.0068 | 0.0009 | 0.0017 | 0.022 | 0.0017 | 0.0019 | 0.0046 | 0.0274 | 0.0038 |
Peroxisomes | 0.0031 | 0.0001 | 0.0001 | 0.0021 | 0.001 | 0.0002 | 0.0018 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.008 | 0.0002 | 0.0005 | 0 | 0.0005 | 0.0001 |
Punctate Nuclear | 0.002 | 0.0002 | 0.0005 | 0.0005 | 0.0004 | 0.0003 | 0.0074 | 0.0001 | 0.0002 | 0.0019 | 0.0002 | 0.0001 | 0.0047 | 0.0031 | 0.0009 | 0.0001 | 0.0243 | 0.0002 |
Vacuole | 0.0907 | 0.0333 | 0.0847 | 0.0914 | 0.0332 | 0.0224 | 0.0204 | 0.0049 | 0.0118 | 0.0288 | 0.0047 | 0.0157 | 0.0505 | 0.0107 | 0.0224 | 0.2515 | 0.0119 | 0.0208 |
Vacuole Periphery | 0.0195 | 0.0021 | 0.0126 | 0.0168 | 0.0157 | 0.0053 | 0.0036 | 0.001 | 0.0031 | 0.0077 | 0.0015 | 0.0034 | 0.0082 | 0.001 | 0.0038 | 0.044 | 0.0049 | 0.0067 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 48.1272 | 46.1708 | 36.3371 | 44.7145 | 36.019 | 40.6704 | 26.7014 | 26.4954 | 25.0442 | 29.6225 |
Translational Efficiency | 1.272 | 1.3451 | 1.5961 | 1.1412 | 1.3206 | 0.8278 | 0.8198 | 0.6362 | 0.6924 | 0.6305 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1134 | 956 | 944 | 1526 | 1559 | 744 | 114 | 2042 | 2693 | 1700 | 1058 | 3568 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 4035.37 | 3057.60 | 2021.54 | 1086.53 | 3547.40 | 2618.80 | 1391.55 | 1207.01 | 3752.88 | 2865.56 | 1953.66 | 1155.48 |
Standard Deviation | 1085.93 | 786.30 | 1319.45 | 301.13 | 818.95 | 689.03 | 397.14 | 374.02 | 971.02 | 776.43 | 1268.28 | 349.86 |
Intensity Change Log 2 | — | -0.400301 | -0.997246 | -1.892973 | — | -0.437856 | -1.350069 | -1.555324 | — | -0.417749 | -1.151647 | -1.725149 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000152 | 0.000374 | 0.000460 | 0.000605 | 0.000099 | 0.000296 | 0.000709 | 0.000358 | 0.000121 | 0.000340 | 0.000487 | 0.000464 |
Bud Neck | 0.000869 | 0.001516 | 0.003531 | 0.002757 | 0.000692 | 0.000895 | 0.002898 | 0.003120 | 0.000767 | 0.001244 | 0.003463 | 0.002965 |
Bud Site | 0.004861 | 0.012978 | 0.025294 | 0.021590 | 0.002952 | 0.013996 | 0.030492 | 0.024750 | 0.003756 | 0.013424 | 0.025854 | 0.023398 |
Cell Periphery | 0.012163 | 0.010458 | 0.006327 | 0.000194 | 0.008072 | 0.007674 | 0.001076 | 0.000221 | 0.009794 | 0.009240 | 0.005761 | 0.000209 |
Cytoplasm | 0.718700* | 0.790875* | 0.792299* | 0.706019* | 0.764083* | 0.857185* | 0.654673* | 0.787675* | 0.744972* | 0.819896* | 0.777470* | 0.752751* |
Cytoplasmic Foci | 0.012479 | 0.016732 | 0.038827 | 0.034068 | 0.009854 | 0.016201 | 0.069568 | 0.027457 | 0.010959 | 0.016499 | 0.042139 | 0.030284 |
Eisosomes | 0.000002 | 0.000003 | 0.000007 | 0.000010 | 0.000001 | 0.000003 | 0.000020 | 0.000006 | 0.000002 | 0.000003 | 0.000008 | 0.000008 |
Endoplasmic Reticulum | 0.001166 | 0.001499 | 0.001398 | 0.000457 | 0.001640 | 0.001116 | 0.001018 | 0.000405 | 0.001440 | 0.001331 | 0.001357 | 0.000427 |
Endosome | 0.001287 | 0.002298 | 0.002603 | 0.006292 | 0.001363 | 0.001768 | 0.009326 | 0.006511 | 0.001331 | 0.002066 | 0.003327 | 0.006417 |
Golgi | 0.076141 | 0.056427 | 0.012610 | 0.003627 | 0.064312 | 0.026817 | 0.005413 | 0.003621 | 0.069293 | 0.043468 | 0.011835 | 0.003624 |
Lipid Particles | 0.012449 | 0.017289 | 0.006330 | 0.001169 | 0.011092 | 0.011317 | 0.004657 | 0.001380 | 0.011663 | 0.014675 | 0.006150 | 0.001290 |
Mitochondria | 0.000211 | 0.000241 | 0.000403 | 0.002086 | 0.000086 | 0.000820 | 0.000017 | 0.001563 | 0.000138 | 0.000495 | 0.000361 | 0.001787 |
Mitotic Spindle | 0.002558 | 0.002953 | 0.006280 | 0.014777 | 0.001304 | 0.004155 | 0.001227 | 0.016954 | 0.001832 | 0.003479 | 0.005736 | 0.016023 |
None | 0.003868 | 0.001373 | 0.002144 | 0.001033 | 0.001341 | 0.000798 | 0.000129 | 0.001026 | 0.002405 | 0.001121 | 0.001927 | 0.001029 |
Nuclear Periphery | 0.000644 | 0.000339 | 0.000357 | 0.000188 | 0.000482 | 0.000332 | 0.000167 | 0.000170 | 0.000550 | 0.000336 | 0.000336 | 0.000178 |
Nuclear Periphery Foci | 0.020899 | 0.014767 | 0.004906 | 0.000908 | 0.017673 | 0.004239 | 0.005559 | 0.000775 | 0.019032 | 0.010159 | 0.004976 | 0.000832 |
Nucleolus | 0.000097 | 0.000250 | 0.000138 | 0.000179 | 0.000053 | 0.000087 | 0.000055 | 0.000253 | 0.000072 | 0.000179 | 0.000129 | 0.000221 |
Nucleus | 0.009923 | 0.007746 | 0.033278 | 0.016606 | 0.007558 | 0.005023 | 0.013132 | 0.011392 | 0.008554 | 0.006554 | 0.031107 | 0.013622 |
Peroxisomes | 0.000020 | 0.000123 | 0.000161 | 0.001168 | 0.000011 | 0.000153 | 0.000734 | 0.001347 | 0.000014 | 0.000136 | 0.000223 | 0.001270 |
Vacuole | 0.048364 | 0.039586 | 0.050318 | 0.182581 | 0.059305 | 0.031411 | 0.196248 | 0.107352 | 0.054698 | 0.036009 | 0.066042 | 0.139527 |
Vacuole Periphery | 0.073148 | 0.022173 | 0.012330 | 0.003685 | 0.048026 | 0.015714 | 0.002883 | 0.003665 | 0.058605 | 0.019346 | 0.011312 | 0.003674 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.12 | -4.90 | -1.45 | -0.48 | 0.16 | -3.71 | -3.67 | -2.28 | 0.12 | 1.90 | -3.86 | -8.79 | -2.35 | -0.26 | 1.41 |
Bud Neck | -2.00 | -6.04 | -5.45 | -4.17 | 2.65 | -1.47 | -4.25 | -7.48 | -6.47 | -1.03 | -2.97 | -7.19 | -9.28 | -7.23 | 2.13 |
Bud Site | -5.52 | -12.01 | -8.30 | -2.52 | 3.84 | -8.50 | -4.38 | -12.88 | -3.63 | 0.77 | -9.78 | -13.64 | -15.20 | -4.47 | 3.70 |
Cell Periphery | 1.28 | 5.00 | 13.54 | 10.34 | 8.11 | 0.33 | 12.66 | 15.25 | 6.69 | 2.38 | 0.64 | 4.88 | 20.04 | 12.18 | 8.16 |
Cytoplasm | -5.55 | -4.35 | 2.43 | 8.77 | 7.59 | -8.44 | 6.18 | 0.17 | 9.59 | -6.22 | -8.76 | -0.79 | 1.84 | 11.55 | 2.34 |
Cytoplasmic Foci | -3.50 | -10.94 | -11.10 | -8.11 | 3.45 | -4.25 | -7.77 | -12.00 | -5.62 | 5.40 | -5.89 | -13.35 | -16.34 | -9.67 | 6.11 |
Eisosomes | -1.69 | -5.72 | -10.15 | -8.16 | -1.91 | -3.05 | -6.96 | -13.99 | -6.77 | 5.47 | -3.23 | -8.83 | -15.93 | -9.94 | 1.99 |
Endoplasmic Reticulum | -1.01 | -0.72 | 4.15 | 4.25 | 3.89 | 1.11 | 1.42 | 3.32 | 2.03 | 2.57 | 0.38 | 0.23 | 4.56 | 4.22 | 3.93 |
Endosome | -4.84 | -5.75 | -7.34 | -5.14 | -4.49 | -1.56 | -4.97 | -6.01 | -5.01 | 2.07 | -4.51 | -7.52 | -8.96 | -6.84 | -3.88 |
Golgi | 4.17 | 17.64 | 20.93 | 16.70 | 7.09 | 10.14 | 19.89 | 23.10 | 9.15 | 0.80 | 8.58 | 24.79 | 31.13 | 18.80 | 7.65 |
Lipid Particles | -2.36 | 4.19 | 9.10 | 10.02 | 7.67 | -0.14 | 4.80 | 11.28 | 7.50 | 3.07 | -2.30 | 5.67 | 14.39 | 12.42 | 7.94 |
Mitochondria | -0.19 | -3.20 | -2.21 | -2.15 | -1.70 | -1.14 | 3.80 | -2.60 | -0.42 | -2.77 | -1.23 | -4.45 | -3.40 | -1.79 | -2.44 |
Mitotic Spindle | -0.82 | -4.63 | -4.99 | -4.73 | -2.55 | -1.88 | 0.69 | -7.14 | -4.50 | -7.26 | -2.32 | -5.85 | -8.70 | -6.75 | -4.75 |
None | 3.04 | 1.96 | 3.52 | 2.23 | 5.46 | 4.87 | 17.67 | 2.52 | -0.92 | -5.60 | 3.64 | 1.04 | 4.03 | 1.34 | 5.62 |
Nuclear Periphery | 3.61 | 2.85 | 5.79 | 1.85 | 3.77 | 0.97 | 3.25 | 3.61 | 0.70 | 0.88 | 2.61 | 2.65 | 6.34 | 1.48 | 3.75 |
Nuclear Periphery Foci | 3.35 | 9.70 | 12.54 | 13.49 | 9.55 | 12.48 | 9.42 | 18.45 | 5.89 | 6.51 | 8.35 | 14.92 | 21.20 | 14.43 | 10.88 |
Nucleolus | -0.94 | -2.76 | -4.12 | 0.49 | -0.71 | -1.20 | -0.47 | -3.11 | -2.58 | -3.04 | -1.14 | -4.53 | -3.82 | -0.54 | -1.95 |
Nucleus | 1.84 | -10.27 | -6.65 | -9.20 | 6.24 | 3.15 | -2.83 | -9.05 | -11.39 | 1.18 | 2.88 | -11.55 | -10.59 | -13.73 | 7.85 |
Peroxisomes | -2.16 | -7.70 | -5.12 | -4.39 | -4.23 | -4.25 | -5.46 | -5.71 | -4.86 | -2.15 | -4.01 | -9.69 | -7.63 | -6.51 | -5.97 |
Vacuole | 2.37 | -1.41 | -22.51 | -24.05 | -20.11 | 6.75 | -8.13 | -15.10 | -20.77 | 5.04 | 6.56 | -4.47 | -26.29 | -31.24 | -14.39 |
Vacuole Periphery | 11.18 | 13.83 | 16.80 | 10.19 | 5.08 | 9.41 | 16.61 | 16.84 | 6.01 | -0.87 | 14.33 | 17.49 | 23.63 | 11.65 | 5.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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