ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF AIM10/YER087W
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0.07 0.05 0.06 0.08 0.06 0.09 0.06 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.45 0.55 0.52 0.6 0.74 0.8 0.77 0.74 0.8 0.77 0.78 0.78 0.38 0.44 0.57 – – – 0 0.06 0.05 0 0 0
Endoplasmic Reticulum 0.52 0.5 0.44 0.26 0.15 0.1 0.1 0.09 0.06 0 0.05 0.07 0.63 0.59 0.46 – – – 0.79 0.77 0.77 0.65 0.7 0.64
Endosome 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.06 0.1 0.11 0.12 0.15 0.17 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0.05 0.05 0.07 0 0.06 0.05 0 0.06 0 0 0.05 0 0 – – – 0.11 0.1 0.1 0.2 0.16 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 11 5 38 16 17 15 9 6 14 8 8 9 2 6 – – – 4 6 5 6 3 8
Bud 2 2 3 14 10 36 27 30 40 33 44 34 2 2 1 – – – 1 1 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 1 3 0 2 1 2
Bud Site 0 0 0 0 0 1 0 1 1 0 1 1 0 0 0 – – – – – – – – –
Cell Periphery 1 3 2 12 10 7 7 8 9 17 17 19 10 10 15 – – – 0 0 0 0 0 0
Cytoplasm 199 249 184 240 337 415 404 382 377 452 375 426 150 224 301 – – – 19 27 18 13 8 9
Endoplasmic Reticulum 227 227 154 104 69 50 53 46 26 25 25 38 250 300 243 – – – 398 382 315 302 281 272
Endosome 1 3 12 20 20 12 9 15 8 10 5 1 2 4 2 – – – 6 9 4 14 10 9
Golgi 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 – – – 3 3 2 10 13 11
Mitochondria 1 1 6 12 6 26 32 49 52 68 72 94 0 0 0 – – – 4 7 13 17 12 10
Nucleus 2 0 0 0 1 1 4 3 1 1 2 2 1 2 1 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2 0 1 5 0 1 1 0 0 2 0 – – – 3 1 1 1 1 0
Nucleolus 0 0 0 1 1 1 0 3 1 0 1 0 0 0 0 – – – 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 39 15 17 19 33 20 30 25 15 35 14 20 18 16 15 – – – 54 48 42 91 62 90
Unique Cell Count 438 454 354 399 458 516 522 514 471 587 483 546 399 508 525 502 498 411 464 401 423
Labelled Cell Count 480 512 385 460 505 586 582 577 536 658 565 643 442 562 584 502 498 411 464 401 423


(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – – – – – – – – – – – – – – – – – – – –
Std Deviation (1e-4) – – – – – – – – – – – – – – – – – – – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 4.8 1.9 1.7 1.4 0.4 0 1.0 0.3 0.1 0.9 0 0 – – –
Bud 2.5 1.5 4.3 3.5 3.8 4.9 3.7 5.1 4.0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 2.5 1.9 0 0 0 0 2.5 2.8 2.8 2.1 0 2.4 – – –
Cytoplasm 2.3 6.4 8.9 7.9 6.8 8.6 7.9 7.8 8.2 -4.0 -2.3 1.6 – – –
Endoplasmic Reticulum -5.0 -9.0 -11.6 -11.4 -11.9 -13.1 -14.9 -13.4 -13.1 5.3 4.5 0.8 – – –
Endosome 1.1 0.7 -0.9 -1.6 -0.4 -1.6 -1.7 -2.4 -3.9 -2.9 -2.8 -3.5 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 1.2 -0.5 2.6 3.2 4.7 5.2 5.5 6.5 7.2 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 1.4 -0.7 0.6 0 -1.2 0.8 -1.4 -0.8 -0.2 -1.2 -1.5 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.768 7.3596 7.2664 6.958 6.2731 6.7754 8.9098 10.3821 10.2353 9.2716 9.5414 9.9181 6.656 7.3867 7.6629 7.0592 7.2856 7.2807
Actin 0.0001 0.0006 0.0007 0.0012 0 0.0002 0.0001 0.0002 0.0003 0.0001 0.0002 0.0001 0.0011 0.0001 0.0057 0.0311 0.0028 0.0002
Bud 0.0034 0.0003 0.0007 0.0142 0.0001 0.0035 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0004 0.0008 0.0003 0.0001
Bud Neck 0.0003 0.0007 0.0005 0.0015 0.0005 0.0015 0.0002 0.0005 0.0003 0.0002 0.001 0.0009 0.0002 0.0004 0.0068 0.0037 0.0007 0.0006
Bud Periphery 0.0015 0.0003 0.0005 0.006 0.0002 0.0016 0.0001 0.0002 0.0002 0 0.0008 0.0002 0.0003 0.0002 0.0006 0.0011 0.0004 0.0002
Bud Site 0.0021 0.0065 0.0051 0.0129 0.0002 0.0009 0.0002 0.0009 0.0002 0 0.0003 0.0002 0.0006 0.0007 0.003 0.0178 0.0008 0.0002
Cell Periphery 0.0013 0.002 0.0009 0.0032 0.0022 0.0013 0.0016 0.0021 0.0023 0.0006 0.0032 0.001 0.0011 0.0047 0.0013 0.0014 0.0008 0.0009
Cytoplasm 0.2238 0.2817 0.2023 0.2774 0.2687 0.244 0.1484 0.1687 0.156 0.0701 0.1656 0.1355 0.1262 0.159 0.0968 0.1249 0.2015 0.0732
Cytoplasmic Foci 0.0067 0.0138 0.0164 0.0096 0.0069 0.0247 0.0058 0.0063 0.0061 0.0014 0.0047 0.005 0.0082 0.0045 0.0082 0.0158 0.0188 0.0036
Eisosomes 0 0 0 0.0003 0 0 0 0 0 0 0 0 0.0002 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.5414 0.4104 0.5461 0.3603 0.4849 0.472 0.6814 0.5898 0.6652 0.7987 0.5003 0.6562 0.651 0.5814 0.6586 0.3903 0.3224 0.6302
Endosome 0.0603 0.0824 0.0833 0.0687 0.0467 0.0665 0.0381 0.049 0.0481 0.0279 0.0553 0.0506 0.056 0.0382 0.069 0.1215 0.1557 0.0644
Golgi 0.0008 0.0025 0.0029 0.0007 0.0004 0.0009 0.001 0.0015 0.0017 0.0004 0.0007 0.0012 0.0057 0.0009 0.0078 0.0195 0.0451 0.0011
Lipid Particles 0.0058 0.0087 0.0085 0.0018 0.0067 0.0037 0.0102 0.0113 0.0172 0.0019 0.0047 0.0065 0.0117 0.0071 0.0078 0.0109 0.0164 0.0032
Mitochondria 0.0006 0.0008 0.0016 0.0034 0.0002 0.0012 0.0002 0.0003 0.0009 0.0008 0.0002 0.0005 0.0086 0.0003 0.0039 0.0034 0.0093 0.0008
None 0.0163 0.0065 0.0069 0.01 0.0025 0.0073 0.0029 0.0033 0.001 0.0003 0.0005 0.0018 0.0149 0.0005 0.0009 0.0012 0.002 0.0005
Nuclear Periphery 0.0381 0.0357 0.0341 0.0324 0.0304 0.0359 0.031 0.0315 0.0212 0.0607 0.0282 0.0235 0.0305 0.0255 0.0244 0.029 0.0464 0.0403
Nucleolus 0.0003 0.0015 0.0003 0.0061 0.0002 0.0047 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0002 0.0012 0.0002 0.0003
Nucleus 0.0131 0.0132 0.0082 0.0153 0.0056 0.017 0.0056 0.0046 0.0033 0.003 0.0071 0.0046 0.0049 0.0051 0.0032 0.006 0.0066 0.0065
Peroxisomes 0 0.0001 0.0001 0.0002 0 0.0001 0 0 0.0001 0 0 0 0.0009 0 0.003 0.0025 0.0028 0
Punctate Nuclear 0.0018 0.0025 0.0016 0.0019 0.0005 0.0084 0.0003 0.0003 0.0004 0.0002 0.0003 0.0003 0.0014 0.0003 0.0005 0.0013 0.0014 0.0004
Vacuole 0.0781 0.1246 0.0742 0.164 0.1347 0.0987 0.07 0.1247 0.0712 0.0271 0.2158 0.1055 0.0723 0.1657 0.0928 0.1997 0.1511 0.1606
Vacuole Periphery 0.0041 0.0051 0.0051 0.009 0.0083 0.0061 0.0027 0.0043 0.0042 0.0064 0.0111 0.0059 0.004 0.0051 0.0049 0.0167 0.0144 0.0127

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
508 1953 2780 306 1773 1317 1408 1238 2281 3270 4188 1544

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 784.34 1002.41 1263.37 1679.81 905.26 975.78 1315.94 1623.88 878.33 991.68 1281.04 1634.96
Standard Deviation 125.40 194.22 179.91 260.01 156.55 146.86 209.50 285.42 158.38 177.16 191.98 281.45
Intensity Change Log 2 — 0.353922 0.687726 1.098747 — 0.108224 0.539690 0.843041 — 0.227499 0.610303 0.967397

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000594 0.000589 0.000441 0.000591 0.000291 0.000824 0.001792 0.001054 0.000359 0.000684 0.000895 0.000962
Bud Neck 0.002611 0.001177 0.002101 0.002965 0.001641 0.001392 0.001186 0.002208 0.001857 0.001264 0.001793 0.002358
Bud Site 0.003425 0.006562 0.002527 0.006329 0.004061 0.005884 0.008670 0.010698 0.003919 0.006289 0.004592 0.009832
Cell Periphery 0.001258 0.001474 0.000328 0.015368 0.000890 0.001714 0.002380 0.018181 0.000972 0.001571 0.001018 0.017624
Cytoplasm 0.238594* 0.085918 0.088235 0.021442 0.076465 0.073323 0.043537 0.023480 0.112573* 0.080845 0.073207 0.023076
Cytoplasmic Foci 0.127411 0.038761 0.021489 0.007261 0.063913 0.034717 0.010531 0.014424 0.078054 0.037132 0.017805 0.013004
Eisosomes 0.000635 0.000264 0.000078 0.000110 0.000170 0.000446 0.000091 0.000145 0.000273 0.000337 0.000082 0.000138
Endoplasmic Reticulum 0.349758* 0.714100* 0.674901* 0.628189* 0.584627* 0.737086* 0.824676* 0.649187* 0.532320* 0.723358* 0.725255* 0.645025*
Endosome 0.022010 0.023987 0.027792 0.041656 0.043694 0.023827 0.023863 0.041985 0.038865 0.023922 0.026471 0.041920
Golgi 0.026137 0.019563 0.004575 0.010105 0.016164 0.021316 0.009525 0.024829 0.018385 0.020269 0.006239 0.021911
Lipid Particles 0.115015* 0.025793 0.030320 0.035429 0.025116 0.023587 0.027074 0.075697 0.045138 0.024905 0.029229 0.067716
Mitochondria 0.008518 0.004446 0.002209 0.000232 0.004927 0.007537 0.000809 0.000676 0.005727 0.005691 0.001738 0.000588
Mitotic Spindle 0.000293 0.000203 0.001216 0.001539 0.000113 0.000302 0.000761 0.000414 0.000153 0.000243 0.001063 0.000637
None 0.003833 0.005725 0.001350 0.000184 0.001807 0.000628 0.001671 0.000223 0.002258 0.003672 0.001458 0.000215
Nuclear Periphery 0.007640 0.001865 0.025460 0.003209 0.003520 0.000369 0.001252 0.000424 0.004438 0.001263 0.017321 0.000976
Nuclear Periphery Foci 0.016692 0.005542 0.041449 0.042834 0.006162 0.002962 0.022541 0.010192 0.008507 0.004503 0.035092 0.016661
Nucleolus 0.001014 0.000230 0.000363 0.000040 0.000536 0.000195 0.000031 0.000047 0.000642 0.000216 0.000251 0.000046
Nucleus 0.029887 0.006014 0.013506 0.000981 0.004650 0.002775 0.000654 0.000368 0.010271 0.004709 0.009185 0.000490
Peroxisomes 0.003794 0.001490 0.000416 0.000111 0.001379 0.002489 0.000360 0.000917 0.001917 0.001892 0.000397 0.000757
Vacuole 0.036556 0.049265 0.058979 0.174358 0.139988 0.051229 0.016947 0.117487 0.116953 0.050056 0.044848 0.128758
Vacuole Periphery 0.004325 0.007031 0.002265 0.007066 0.019884 0.007398 0.001649 0.007363 0.016419 0.007179 0.002058 0.007304

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.03 1.00 -0.03 -0.05 -0.52 -3.59 -3.46 -3.96 -1.26 1.50 -3.24 -3.36 -3.71 -1.78 -0.38
Bud Neck 3.27 0.96 -1.19 -3.08 -1.82 1.04 1.86 -2.21 -3.02 -3.73 2.95 -0.07 -2.39 -4.62 -2.45
Bud Site -2.81 1.28 -2.73 -0.59 -3.41 -1.94 -3.88 -5.38 -3.57 -1.37 -3.12 -1.54 -5.93 -3.43 -5.03
Cell Periphery -0.88 5.63 -4.59 -4.51 -4.91 -4.19 -4.57 -12.85 -12.15 -11.41 -3.88 -0.45 -13.48 -12.92 -13.40
Cytoplasm 14.43 14.19 19.59 12.09 13.08 0.73 8.35 15.25 13.00 6.11 7.70 9.50 22.82 20.04 18.43
Cytoplasmic Foci 12.60 15.44 17.53 12.88 8.22 8.13 17.55 15.88 7.98 -2.28 13.10 20.91 22.25 12.99 3.68
Eisosomes 3.24 5.21 4.79 2.65 -0.66 -4.78 2.96 0.96 5.51 -2.99 -1.40 5.68 4.28 5.36 -2.73
Endoplasmic Reticulum -21.60* -19.22 -9.87 4.66 2.69 -12.68 -21.79 -4.59 7.20 14.94 -20.19 -20.42 -8.91 8.25 8.14
Endosome -0.99 -3.06 -5.32 -5.09 -3.93 8.69 9.07 0.61 -8.31 -8.70 8.84 7.19 -1.60 -10.48 -8.90
Golgi 2.41 8.90 5.63 4.68 -3.42 -2.57 4.78 -3.73 -1.32 -7.00 -1.31 11.10 -1.79 -0.82 -9.20
Lipid Particles 12.96 12.20 9.29 -1.83 -0.90 0.71 -0.72 -11.20 -11.48 -10.01 8.56 6.30 -5.47 -11.41 -9.99
Mitochondria 2.29 3.64 4.83 8.71 7.51 -1.80 8.71 9.37 4.98 0.73 0.05 7.31 9.87 8.14 6.09
Mitotic Spindle 0.86 -4.01 -2.72 -2.85 -0.89 -1.84 -2.97 -1.81 -0.63 1.34 -1.42 -5.40 -3.08 -2.43 1.68
None -1.34 2.36 3.47 5.91 5.75 2.17 0.21 3.02 2.61 3.09 -1.94 1.61 4.34 6.14 6.20
Nuclear Periphery 4.66 -9.40 2.81 -1.11 11.61 6.22 4.06 6.11 -1.00 3.46 5.44 -11.25 6.70 0.62 15.25
Nuclear Periphery Foci 6.00 -10.02 -4.89 -7.05 -0.51 2.96 -8.26 -3.50 -6.44 6.03 4.54 -17.12 -5.66 -8.85 9.36
Nucleolus 3.39 2.74 4.20 7.82 7.17 3.06 7.33 7.17 1.63 -1.36 5.14 4.84 8.05 4.36 6.88
Nucleus 9.25 6.36 11.87 4.78 14.10 2.28 8.28 8.71 3.39 1.90 5.93 0.99 14.00 6.48 15.67
Peroxisomes 5.68 9.00 9.93 8.02 5.04 -3.87 6.49 2.61 5.50 -3.39 0.16 10.92 7.01 6.38 -2.96
Vacuole -3.14 -6.29 -9.66 -8.86 -8.13 14.61 23.66 2.56 -10.46 -17.66 14.68 16.20 -2.46 -14.21 -15.24
Vacuole Periphery -4.06 3.96 -2.31 -0.08 -4.31 10.30 17.67 10.69 -0.01 -9.45 10.34 17.56 9.48 -0.27 -9.99
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF AIM10/YER087W
Localization
Cell Percentages ER (81%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

YER087C-A

YER087C-A


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yer087c-a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available