Standard name
Human Ortholog
Description Protein involved in rRNA and ribosome biogenesis; activated in stochastic pulses of nuclear localization; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; relative distribution to the nucleus increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.94 0.14 0.72 0.6 0.66 0.46 0.49 0.36 0.48 0.18 0.37 0.49 0.83 0.89 0.94 0.94 0.91 0.86 0.88 0.82 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.09 0.07 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.16 0.14 0.33 0.34 0.55 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0.73 0.11 0.09 0.05 0.15 0.16 0.12 0.21 0.69 0.49 0.37 0 0 0 0 0 0 0 0.06 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.14 0.21 0.19 0.17 0.17 0.15 0.13 0.1 0.17 0.15 0.13 0.21 0.12 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0
Bud 3 2 0 1 0 1 5 4 3 6 4 0 0 0 0 0 0 0 1 1 0 4 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Bud Site 0 0 0 0 0 0 0 0 2 2 4 0 0 0 0 0 0
Cell Periphery 1 5 9 0 2 0 2 1 0 0 1 0 0 0 4 8 4 0 0 0 0 0 0
Cytoplasm 156 332 146 25 158 143 185 111 127 118 143 22 56 85 89 121 98 150 316 141 49 114 137
Endoplasmic Reticulum 0 2 3 1 0 0 0 0 0 0 2 0 0 2 8 2 4 2 2 4 0 0 2
Endosome 0 2 0 7 8 21 20 9 11 8 4 5 4 8 1 0 0 1 4 1 0 0 1
Golgi 1 0 2 0 0 0 0 0 0 0 0 0 0 0 3 2 1 0 0 0 0 0 0
Mitochondria 4 0 0 0 1 38 40 79 87 179 108 0 1 0 0 1 0 1 0 2 1 2 1
Nucleus 1 0 4 134 25 22 15 37 41 38 63 84 75 65 1 3 2 0 3 1 2 8 11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 2 0 0 0 2 2 3 2 1 3 0 0 0 1 0 0 0 0 0 2
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 0 2 2 2 2 4 0 1 1 0 0 0 0 1 0 0 2 4
Vac/Vac Membrane 0 5 3 26 45 46 47 42 39 44 29 21 23 23 23 16 7 0 8 2 0 1 3
Unique Cell Count 161 340 155 183 219 240 279 242 259 326 299 122 152 174 107 136 104 161 346 164 56 139 181
Labelled Cell Count 166 349 168 197 239 271 317 287 314 401 364 133 163 185 129 153 117 161 346 164 56 139 181


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 9.9 8.5 8.5 7.7 5.9 5.9 5.2 5.1 4.6 4.7 8.7 8.5 7.5 11.7 11.9 11.4 8.2 8.4
Std Deviation (1e-4) 0.9 1.4 1.4 2.0 1.7 1.4 1.5 1.6 1.3 1.0 1.1 1.4 1.8 1.5 2.4 2.4 2.1 1.2 1.7
Intensity Change (Log2) 0.0 -0.15 -0.53 -0.52 -0.69 -0.73 -0.88 -0.84 0.04 -0.0 -0.17 0.47 0.49 0.42 -0.04 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 -0.8 0 -0.7
Cytoplasm -14.8 -5.4 -7.6 -6.5 -9.8 -9.4 -11.9 -9.7 -12.9 -10.6 -9.0 -2.9 -1.6 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 3.8 3.4 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.2 4.9 7.9 8.1 0 0 0 0 0 0 0 0
Nucleus 13.2 3.1 2.6 1.4 4.1 4.2 3.3 5.3 11.8 9.4 7.7 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 4.0 5.3 5.1 4.7 4.7 4.3 4.0 3.1 4.5 4.2 3.8 5.2 3.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6476 1.7378 1.4725 1.5875 0.909 1.9452 2.1842 2.8272 2.5393 1.9228 1.9376 2.4551 3.1166 3.827 3.1413 3.5667 3.1434 3.4802
Actin 0.0454 0.0012 0.0034 0.0001 0.0073 0.0055 0.0103 0.0008 0.0126 0.0037 0.006 0.0006 0.0102 0.001 0.0034 0.04 0.0381 0.004
Bud 0.0017 0.0047 0.0018 0.0004 0.0007 0.0014 0.0019 0.004 0.0065 0.0002 0.0058 0.0015 0.0038 0.0044 0.0049 0.0032 0.0006 0.002
Bud Neck 0.0036 0.0008 0.001 0.0011 0.0043 0.0059 0.0079 0.0013 0.0021 0.0011 0.0016 0.0043 0.0029 0.001 0.0024 0.0097 0.0029 0.0083
Bud Periphery 0.0017 0.0015 0.0011 0.0003 0.0011 0.0011 0.0012 0.0013 0.0032 0.0001 0.0024 0.0008 0.0052 0.0022 0.0021 0.0037 0.0007 0.0018
Bud Site 0.0061 0.0071 0.0017 0.0004 0.0007 0.0011 0.0055 0.0252 0.0046 0.0005 0.0017 0.0007 0.0046 0.0099 0.0042 0.009 0.0012 0.0015
Cell Periphery 0.0005 0.0003 0.0001 0.0002 0.0002 0.0001 0.0005 0.0002 0.0005 0.0001 0.0001 0.0001 0.0006 0.0002 0.0002 0.0024 0.0001 0.0001
Cytoplasm 0.5342 0.6442 0.6103 0.6451 0.5547 0.5876 0.4884 0.4786 0.5243 0.5479 0.3666 0.5499 0.5182 0.6125 0.5857 0.4877 0.4333 0.4494
Cytoplasmic Foci 0.023 0.014 0.0101 0.0817 0.0175 0.0096 0.0351 0.008 0.0134 0.0481 0.0465 0.0107 0.0264 0.0109 0.0133 0.0168 0.0179 0.0069
Eisosomes 0.0009 0 0 0 0.0001 0 0.0001 0 0.0001 0.0002 0.0001 0 0.0002 0 0 0.0003 0.0005 0
Endoplasmic Reticulum 0.0079 0.0089 0.005 0.0032 0.0034 0.0058 0.009 0.0029 0.0035 0.003 0.005 0.003 0.0045 0.0018 0.0033 0.0087 0.0057 0.0021
Endosome 0.0251 0.008 0.016 0.0419 0.0661 0.0124 0.0241 0.0031 0.0081 0.0111 0.0288 0.0069 0.0202 0.0049 0.0149 0.0193 0.0252 0.0077
Golgi 0.0116 0.0009 0.0093 0.0013 0.032 0.0049 0.0039 0.0002 0.0038 0.0042 0.0175 0.0002 0.005 0.0004 0.0024 0.0108 0.0067 0.0034
Lipid Particles 0.0082 0.0006 0.0013 0.0092 0.0171 0.0068 0.0144 0.0001 0.0049 0.0061 0.0393 0.0001 0.008 0.0001 0.0048 0.0044 0.0222 0.005
Mitochondria 0.0102 0.0004 0.0102 0.0003 0.0317 0.0146 0.0027 0.0008 0.004 0.001 0.0216 0.0006 0.0045 0.0005 0.0023 0.0052 0.0048 0.0025
None 0.0391 0.0477 0.064 0.0059 0.0664 0.0141 0.068 0.1466 0.0717 0.0908 0.0457 0.039 0.0276 0.0323 0.0243 0.0267 0.054 0.0141
Nuclear Periphery 0.0186 0.0128 0.015 0.0068 0.0097 0.0173 0.0114 0.0054 0.0071 0.0052 0.0184 0.0091 0.0076 0.0047 0.0064 0.0107 0.0311 0.0064
Nucleolus 0.0018 0.0013 0.001 0.0012 0.0117 0.0041 0.0022 0.0018 0.0041 0.0046 0.0022 0.0031 0.0042 0.0021 0.0019 0.0026 0.0054 0.0034
Nucleus 0.2124 0.2291 0.2258 0.1671 0.1639 0.2926 0.2739 0.283 0.2891 0.2288 0.3231 0.3441 0.3092 0.2925 0.2954 0.3116 0.3058 0.4665
Peroxisomes 0.0116 0.0027 0.0014 0.0021 0.0012 0.0023 0.0151 0.0003 0.0077 0.0362 0.0303 0.0002 0.0154 0.0008 0.0042 0.0109 0.0076 0.0026
Punctate Nuclear 0.0225 0.0076 0.0159 0.0016 0.0021 0.007 0.0171 0.0338 0.0177 0.0051 0.0317 0.0212 0.0126 0.0138 0.0177 0.008 0.0321 0.0088
Vacuole 0.012 0.0056 0.0043 0.0254 0.0051 0.0048 0.0063 0.0022 0.01 0.0015 0.0043 0.0028 0.008 0.0035 0.0051 0.007 0.003 0.003
Vacuole Periphery 0.0019 0.0005 0.001 0.0048 0.0029 0.001 0.0011 0.0003 0.001 0.0003 0.0012 0.001 0.0011 0.0005 0.001 0.0014 0.001 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.7478 22.5401 23.9978 26.0016 26.2954 23.0091 21.0563 23.2401 21.3822 26.4608
Translational Efficiency 1.0061 1.0368 0.8789 0.6495 0.977 0.8369 0.8926 0.796 0.983 0.9209

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein involved in rRNA and ribosome biogenesis; activated in stochastic pulses of nuclear localization; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (79%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Dot6

Dot6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dot6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available