Standard name | |
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Human Ortholog | |
Description | Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 0 | 5 | 1 | 9 | 7 | 1 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 287 | 173 | 121 | 116 | 116 | 127 | 131 | 142 | 119 | 73 | 100 | 138 | 288 | 351 | 488 | 347 | 224 | 209 | 298 | 164 | 118 | 221 | 287 | 273 |
Endoplasmic Reticulum | 2 | 1 | 2 | 1 | 1 | 6 | 5 | 5 | 5 | 6 | 1 | 8 | 3 | 4 | 2 | 9 | 6 | 6 | 0 | 0 | 1 | 0 | 5 | 3 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Mitochondria | 1 | 1 | 0 | 2 | 0 | 7 | 8 | 5 | 17 | 11 | 28 | 25 | 2 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 2 |
Nucleus | 4 | 0 | 0 | 1 | 1 | 2 | 1 | 4 | 9 | 4 | 4 | 7 | 0 | 1 | 1 | 6 | 3 | 1 | 2 | 4 | 2 | 0 | 1 | 5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 8 | 2 | 2 | 4 | 5 | 3 | 10 | 5 | 6 | 6 | 8 | 4 | 8 | 3 | 1 | 44 | 56 | 18 | 2 | 1 | 1 | 0 | 2 | 9 |
Unique Cell Count | 299 | 176 | 124 | 122 | 122 | 142 | 151 | 157 | 150 | 97 | 140 | 181 | 299 | 356 | 491 | 378 | 258 | 221 | 312 | 177 | 129 | 228 | 307 | 314 |
Labelled Cell Count | 303 | 177 | 126 | 124 | 125 | 147 | 161 | 162 | 163 | 101 | 154 | 193 | 302 | 361 | 493 | 410 | 294 | 238 | 312 | 177 | 129 | 228 | 307 | 314 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.1 | 15.7 | 11.5 | 11.6 | 12.7 | 11.5 | 9.6 | 10.8 | 10.6 | 10.6 | 9.8 | 10.2 | 19.5 | 18.4 | 17.2 | 12.7 | 15.7 | 17.6 | 15.7 | 14.9 | 15.5 |
Std Deviation (1e-4) | 2.4 | 3.2 | 2.1 | 2.5 | 3.0 | 2.7 | 2.5 | 2.2 | 2.1 | 2.1 | 2.2 | 2.1 | 3.2 | 3.2 | 2.9 | 2.5 | 3.4 | 3.1 | 3.1 | 3.1 | 3.7 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.15 | 0.01 | -0.26 | -0.08 | -0.11 | -0.11 | -0.22 | -0.17 | 0.77 | 0.69 | 0.59 | 0.15 | 0.46 | 0.62 | 0.46 | 0.38 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | -1.0 | -2.6 | -3.2 | -2.4 | -4.6 | -5.0 | -5.7 | -5.1 | -0.7 | 0.8 | 1.8 | -2.2 | -3.3 | -1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.9 | 0 | 5.3 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 5.1 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.6423 | 28.9771 | 26.1507 | 25.7604 | 23.4439 | 25.6425 | 19.5181 | 25.26 | 22.6803 | 21.3166 | 20.8206 | 22.6692 | 23.535 | 30.8021 | 27.1421 | 25.4709 | 28.8742 | 27.4701 |
Actin | 0.0042 | 0.0001 | 0.0175 | 0.0001 | 0.009 | 0.0096 | 0.0029 | 0.0002 | 0.0024 | 0.0002 | 0.005 | 0.0001 | 0.0251 | 0.0002 | 0.0154 | 0.0002 | 0.0001 | 0.0075 |
Bud | 0.0022 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0023 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0012 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.001 | 0.0002 | 0.0004 | 0.0003 | 0.0014 | 0.0014 | 0.0009 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0005 | 0.001 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0008 |
Bud Periphery | 0.0013 | 0.0001 | 0.0006 | 0 | 0.0003 | 0.0001 | 0.0009 | 0 | 0.0005 | 0 | 0 | 0 | 0.0006 | 0 | 0.0007 | 0 | 0 | 0 |
Bud Site | 0.0008 | 0.0001 | 0.0022 | 0 | 0.0003 | 0.0001 | 0.0016 | 0 | 0.0004 | 0 | 0.0004 | 0 | 0.0006 | 0 | 0.0006 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.825 | 0.9453 | 0.8525 | 0.9072 | 0.8159 | 0.8025 | 0.8419 | 0.9807 | 0.9313 | 0.96 | 0.8266 | 0.9256 | 0.8011 | 0.9592 | 0.8699 | 0.9036 | 0.863 | 0.8107 |
Cytoplasmic Foci | 0.0125 | 0.0004 | 0.0144 | 0.0002 | 0.001 | 0.0039 | 0.0185 | 0.0003 | 0.0043 | 0.0005 | 0.0197 | 0.0003 | 0.0166 | 0.0002 | 0.0091 | 0.0609 | 0.0001 | 0.0007 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0046 | 0.0005 | 0.0011 | 0.0003 | 0.0092 | 0.0031 | 0.0061 | 0.0002 | 0.0014 | 0.0005 | 0.0004 | 0.0005 | 0.0027 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.001 |
Endosome | 0.0086 | 0.0005 | 0.0056 | 0.0001 | 0.0028 | 0.0048 | 0.017 | 0.0001 | 0.0058 | 0.0002 | 0.0008 | 0.0002 | 0.0061 | 0 | 0.0033 | 0.0005 | 0 | 0.0006 |
Golgi | 0.0018 | 0.0001 | 0.0025 | 0 | 0.0015 | 0.0035 | 0.0072 | 0 | 0.0045 | 0 | 0.0034 | 0 | 0.0044 | 0 | 0.0024 | 0.0002 | 0 | 0.0011 |
Lipid Particles | 0.0038 | 0.0007 | 0.0069 | 0 | 0.005 | 0.01 | 0.0026 | 0 | 0.0008 | 0 | 0.001 | 0 | 0.0148 | 0 | 0.0067 | 0.0016 | 0 | 0.0007 |
Mitochondria | 0.0107 | 0.0034 | 0.0022 | 0.0001 | 0.0186 | 0.0024 | 0.0038 | 0.0001 | 0.002 | 0.0001 | 0.0005 | 0.0001 | 0.0029 | 0.0001 | 0.0042 | 0.0001 | 0.0001 | 0.0003 |
None | 0.0066 | 0.0002 | 0.0005 | 0.0002 | 0.001 | 0.0025 | 0.004 | 0.0003 | 0.001 | 0.0003 | 0.0002 | 0.0002 | 0.0018 | 0.0002 | 0.0019 | 0.0004 | 0.0001 | 0.0003 |
Nuclear Periphery | 0.0172 | 0.0041 | 0.0056 | 0.0049 | 0.0223 | 0.0205 | 0.0088 | 0.0014 | 0.0027 | 0.003 | 0.0189 | 0.0052 | 0.0168 | 0.0028 | 0.0039 | 0.0027 | 0.0066 | 0.0126 |
Nucleolus | 0.0006 | 0.0004 | 0.0006 | 0 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0011 | 0 | 0.0004 | 0 | 0 | 0.0001 |
Nucleus | 0.0834 | 0.0415 | 0.0803 | 0.086 | 0.1065 | 0.129 | 0.0588 | 0.0163 | 0.0397 | 0.0341 | 0.0689 | 0.0667 | 0.0815 | 0.0365 | 0.0649 | 0.0255 | 0.1291 | 0.1616 |
Peroxisomes | 0.0035 | 0.0002 | 0.0019 | 0 | 0.0003 | 0.0014 | 0.0175 | 0 | 0.0006 | 0 | 0.0526 | 0 | 0.0128 | 0 | 0.0106 | 0.001 | 0 | 0.0003 |
Punctate Nuclear | 0.0073 | 0.0002 | 0.0022 | 0.0004 | 0.0006 | 0.0029 | 0.0016 | 0.0002 | 0.0007 | 0.0005 | 0.0005 | 0.0004 | 0.0082 | 0.0003 | 0.0021 | 0.0023 | 0.0002 | 0.0009 |
Vacuole | 0.0034 | 0.0012 | 0.0018 | 0.0002 | 0.0016 | 0.0012 | 0.0027 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0003 | 0.0008 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0004 |
Vacuole Periphery | 0.0013 | 0.0007 | 0.0005 | 0.0001 | 0.0018 | 0.0007 | 0.0009 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0004 | 0 | 0 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 108.363 | 102.5266 | 96.0171 | 89.9287 | 114.3464 | 88.5397 | 97.4089 | 86.1312 | 107.299 | 101.0509 |
Translational Efficiency | 0.6835 | 0.7275 | 0.7832 | 0.8977 | 0.6936 | 0.772 | 0.6465 | 0.8321 | 0.7405 | 0.6923 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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