Standard name
Human Ortholog
Description Anthranilate synthase; catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 0.08 0.09 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.99 0.98 0.97 0.91 0.84 0.84 0.77 0.83 0.83 0.99 0.98 1.0 0.99 0.98 0.99 0.98 0.95 0.9 0.97 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.09 0.07 0.09 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0.07 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 6 3 1 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 3 1 1 1 0 0 0 1 2 2 2 0 0 1 0 0 0 0 0 2 2 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 5 3 0 3 0 2 12 16 16 14 24 1 4 5 1 3 1 0 0 0 0 0 0
Cytoplasm 398 266 184 92 130 135 186 169 178 143 164 173 210 294 281 392 304 188 379 244 169 182 171 197
Endoplasmic Reticulum 0 0 1 1 1 2 10 18 14 17 9 7 2 2 0 15 6 10 3 1 3 1 2 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Mitochondria 1 2 2 0 1 2 6 2 14 13 15 16 0 1 0 2 1 0 0 1 1 0 0 0
Nucleus 1 1 0 0 0 0 1 1 2 1 0 2 0 0 0 0 0 1 0 1 0 0 1 0
Nuclear Periphery 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 0 2 2 0 0 1 6 13 0 5 6 3 0 0 1 2 5 0 0 2 3 0 0 2
Unique Cell Count 401 270 189 93 133 139 205 202 212 185 197 209 212 300 282 395 311 189 388 258 188 188 183 212
Labelled Cell Count 404 278 193 94 135 142 213 216 233 200 211 231 213 303 287 412 319 200 388 258 188 188 183 212


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 51.5 47.0 33.2 30.6 28.1 23.9 17.7 18.5 17.0 15.7 15.6 14.7 45.6 44.4 43.4 54.4 65.6 69.9 44.3 42.5 45.6
Std Deviation (1e-4) 9.8 13.3 11.1 10.0 7.8 6.4 5.1 5.4 4.5 4.3 4.4 3.6 10.1 10.3 10.5 16.7 18.9 21.3 9.5 11.8 13.5
Intensity Change (Log2) – – – -0.12 -0.24 -0.47 -0.91 -0.84 -0.97 -1.08 -1.09 -1.17 0.46 0.42 0.39 0.71 0.98 1.07 0.41 0.36 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.2 2.8 3.1 2.6 3.9 0 0 0 0 0 0
Cytoplasm 0.9 0.2 -0.1 -2.7 -4.6 -4.5 -5.9 -4.7 -4.8 1.3 0.5 2.2 1.8 0.3 1.6
Endoplasmic Reticulum 0 0 0 2.6 3.9 3.2 3.9 0 0 0 0 0 2.3 0 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.8 2.9 3.1 3.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.8 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0074 0.0003 0.0014 0.0024 0.0022 0.0005 0.0107 0.0001 0.0277 0 0.0459 0.0021 – – – – – –
Bud 0.0015 0.0001 0.0002 0.0007 0.0002 0.0002 0.0002 0 0.0006 0 0.0006 0 – – – – – –
Bud Neck 0.0006 0.0003 0.0003 0.0005 0.0006 0.0008 0.0003 0.0001 0.0003 0.0001 0.0003 0.0003 – – – – – –
Bud Periphery 0.0011 0 0 0.0003 0.0003 0 0.0001 0 0.0004 0 0.0006 0 – – – – – –
Bud Site 0.0028 0.0002 0.0016 0.0006 0.0001 0 0.0018 0 0.0017 0 0.0011 0 – – – – – –
Cell Periphery 0.0007 0.0001 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0002 0 – – – – – –
Cytoplasm 0.8998 0.9864 0.9915 0.9582 0.9269 0.9935 0.9286 0.9987 0.9177 0.9976 0.8542 0.9774 – – – – – –
Cytoplasmic Foci 0.0024 0.0004 0.0005 0.0012 0.0007 0.0004 0.0117 0.0001 0.0093 0.0003 0.0155 0.0011 – – – – – –
Eisosomes 0.0002 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0 – – – – – –
Endoplasmic Reticulum 0.002 0.0007 0.0003 0.0031 0.0057 0.0002 0.0021 0.0001 0.0025 0.0001 0.0032 0.0014 – – – – – –
Endosome 0.002 0.0001 0.0001 0.0044 0.0238 0.0002 0.0057 0 0.0118 0.0001 0.0119 0.0046 – – – – – –
Golgi 0.0008 0 0.0001 0.0005 0.0232 0 0.0017 0 0.0077 0 0.0071 0.007 – – – – – –
Lipid Particles 0.0012 0.0001 0.0001 0.0001 0.0002 0 0.003 0 0.0015 0 0.0057 0.0019 – – – – – –
Mitochondria 0.0138 0.0002 0.0001 0.0048 0.0082 0.0003 0.0014 0 0.0056 0 0.0048 0.0009 – – – – – –
None 0.0029 0.0042 0.0003 0.0003 0.0001 0.0001 0.0091 0.0001 0.0045 0.0001 0.0098 0.0001 – – – – – –
Nuclear Periphery 0.012 0.0024 0.0006 0.0083 0.0022 0.0005 0.0037 0.0001 0.0012 0.0003 0.012 0.0006 – – – – – –
Nucleolus 0.0032 0.0003 0 0.0001 0 0 0.0005 0 0.0007 0 0.0033 0 – – – – – –
Nucleus 0.0227 0.0028 0.0022 0.0112 0.0028 0.0027 0.0042 0.0006 0.002 0.0013 0.0088 0.002 – – – – – –
Peroxisomes 0.0005 0 0 0.0002 0 0 0.0041 0 0.001 0 0.0017 0.0001 – – – – – –
Punctate Nuclear 0.0065 0.0004 0.0001 0.0003 0 0 0.0095 0 0.0007 0 0.0069 0.0001 – – – – – –
Vacuole 0.0087 0.0005 0.0004 0.0017 0.0016 0.0003 0.0007 0 0.0018 0.0001 0.0032 0.0002 – – – – – –
Vacuole Periphery 0.0073 0.0003 0.0001 0.0011 0.0011 0.0001 0.0003 0 0.001 0 0.0031 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 82.1761 79.6091 79.2448 108.3353 98.5658 74.5326 107.8144 101.5873 97.9861 107.1245
Translational Efficiency 2.7095 2.8933 2.6101 1.8379 2.0687 3.0222 2.0824 1.8978 1.8458 1.7498

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1140 159 778 1896 1935 859 201 671 3075 1018 979 2567

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2523.97 4549.78 3488.83 2765.29 3233.44 4002.79 2840.16 2657.83 2970.42 4088.22 3355.65 2737.20
Standard Deviation 537.17 1012.37 717.19 472.71 605.88 853.62 393.40 453.19 674.83 902.42 713.57 470.06
Intensity Change Log 2 — 0.850102 0.467049 0.131736 — 0.307936 -0.187098 -0.282821 — 0.570938 0.136555 -0.086297

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000081 0.000218 0.000182 0.000271 0.000051 0.000100 0.000494 0.000482 0.000062 0.000119 0.000246 0.000327
Bud Neck 0.000382 0.002036 0.004914 0.001192 0.000287 0.000668 0.007370 0.002428 0.000322 0.000882 0.005418 0.001515
Bud Site 0.001943 0.005652 0.003284 0.006252 0.000885 0.001096 0.006281 0.013471 0.001277 0.001808 0.003899 0.008139
Cell Periphery 0.000466 0.007656 0.002090 0.000961 0.000411 0.000940 0.001534 0.000542 0.000431 0.001989 0.001976 0.000852
Cytoplasm 0.933101* 0.821102* 0.741468* 0.893872* 0.929228* 0.881078* 0.682306* 0.831424* 0.930664* 0.871710* 0.729322* 0.877548*
Cytoplasmic Foci 0.005431 0.019716 0.017100 0.004565 0.004676 0.007673 0.024621 0.012797 0.004956 0.009554 0.018645 0.006717
Eisosomes 0.000001 0.000003 0.000003 0.000002 0.000000 0.000001 0.000006 0.000004 0.000000 0.000001 0.000004 0.000003
Endoplasmic Reticulum 0.000422 0.000071 0.000129 0.000175 0.000064 0.000058 0.000325 0.000188 0.000196 0.000060 0.000169 0.000178
Endosome 0.000333 0.000333 0.001655 0.000948 0.000152 0.000217 0.003133 0.002453 0.000219 0.000235 0.001958 0.001342
Golgi 0.003634 0.006480 0.004526 0.002240 0.003204 0.002977 0.003944 0.008383 0.003364 0.003525 0.004406 0.003846
Lipid Particles 0.001543 0.010913 0.005263 0.003782 0.001895 0.002132 0.008937 0.003306 0.001765 0.003504 0.006018 0.003657
Mitochondria 0.000051 0.000054 0.000200 0.000204 0.000055 0.000113 0.000095 0.000928 0.000054 0.000104 0.000179 0.000393
Mitotic Spindle 0.001319 0.000878 0.003014 0.002920 0.000844 0.001322 0.001269 0.002681 0.001020 0.001252 0.002655 0.002858
None 0.007131 0.007330 0.003123 0.002536 0.009283 0.008580 0.002695 0.002951 0.008485 0.008385 0.003035 0.002644
Nuclear Periphery 0.000058 0.000320 0.000768 0.000067 0.000055 0.000159 0.000609 0.000117 0.000056 0.000184 0.000735 0.000080
Nuclear Periphery Foci 0.004014 0.007304 0.004821 0.002234 0.002546 0.003465 0.008361 0.002424 0.003090 0.004065 0.005548 0.002284
Nucleolus 0.000039 0.000082 0.000280 0.000115 0.000043 0.000378 0.000208 0.000252 0.000041 0.000331 0.000265 0.000151
Nucleus 0.032000 0.070805 0.115682 0.042095 0.038422 0.078224 0.085759 0.053041 0.036041 0.077065 0.109539 0.044956
Peroxisomes 0.000011 0.000029 0.000026 0.000064 0.000013 0.000016 0.000080 0.000201 0.000012 0.000018 0.000037 0.000100
Vacuole 0.006070 0.038019 0.088741 0.034568 0.006427 0.009386 0.160787 0.059510 0.006294 0.013858 0.103533 0.041088
Vacuole Periphery 0.001971 0.000997 0.002731 0.000937 0.001460 0.001417 0.001186 0.002417 0.001649 0.001351 0.002414 0.001324

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.37 -8.31 -4.05 -0.95 -0.11 -3.23 -8.51 -3.53 -2.75 3.39 -4.38 -12.06 -5.33 -3.76 1.21
Bud Neck -4.80 -13.54 -9.93 2.17 11.43 -3.03 -9.57 -11.25 -8.96 6.04 -4.89 -16.37 -15.79 -6.29 12.64
Bud Site -2.74 -4.65 -6.17 -0.42 -1.23 -1.58 -9.41 -5.59 -5.40 -0.93 -2.05 -9.09 -9.65 -8.31 -1.78
Cell Periphery -4.44 -7.34 -4.84 4.18 5.62 -5.48 -5.74 -1.00 4.45 5.34 -5.88 -8.61 -5.27 4.63 6.86
Cytoplasm 8.86 28.70 16.58 -4.03 -20.76 10.44 18.75 19.31 12.08 -9.12 13.64 34.12 24.96 5.21 -21.63
Cytoplasmic Foci -5.95 -10.66 -1.93 5.57 10.09 -4.62 -9.61 -7.26 -4.27 5.89 -7.00 -14.02 -6.79 2.21 11.22
Eisosomes -5.28 -11.90 -8.80 2.22 5.86 -3.40 -10.27 -7.06 -5.82 6.06 -5.94 -15.37 -11.41 -5.48 7.86
Endoplasmic Reticulum 1.89 1.34 1.57 -0.57 0.83 0.33 -5.36 -0.88 -0.98 3.93 1.96 -0.14 1.02 -0.92 1.76
Endosome -0.25 -3.56 -1.53 -1.34 2.61 -0.97 -8.03 -3.29 -3.04 3.55 -0.41 -5.80 -3.68 -3.30 3.47
Golgi -1.96 -1.82 3.58 3.49 5.51 0.40 -0.35 -0.06 -0.19 0.04 -0.30 -2.57 2.41 2.26 3.90
Lipid Particles -4.48 -7.64 -6.17 2.95 3.14 -0.58 -6.84 -5.02 -4.04 4.77 -3.31 -9.49 -7.04 -1.81 4.82
Mitochondria -1.00 -8.94 -1.56 -1.32 2.47 -1.85 -3.50 -1.47 -1.13 -1.20 -1.95 -9.63 -2.09 -1.14 0.79
Mitotic Spindle 0.69 -2.78 -0.90 -1.69 2.07 -1.02 -3.23 -2.20 -1.21 -0.12 -0.56 -3.61 -2.05 -1.36 2.03
None 0.36 8.40 9.71 2.84 3.29 0.92 10.79 9.71 8.44 -0.33 0.44 13.11 14.03 9.96 2.39
Nuclear Periphery -4.84 -11.05 -3.02 4.34 10.73 -4.09 -11.20 -10.74 -1.05 8.95 -5.69 -12.85 -9.11 3.18 12.05
Nuclear Periphery Foci -2.92 -2.56 1.55 4.51 6.92 -2.24 -8.89 -3.53 -0.09 7.43 -2.40 -7.75 0.19 2.93 9.23
Nucleolus -4.96 -14.11 -13.54 -3.08 10.39 -1.13 -9.41 -7.06 0.54 1.07 -1.16 -15.99 -13.76 0.74 9.62
Nucleus -5.68 -19.57 -9.20 3.33 15.96 -12.41 -7.70 -6.51 4.42 4.49 -14.36 -20.49 -10.32 8.26 16.26
Peroxisomes -2.98 -5.05 -8.92 -4.10 -1.42 -0.41 -7.58 -3.96 -3.81 -0.10 -1.03 -7.88 -7.48 -6.04 -1.33
Vacuole -5.88 -17.92 -21.58 -1.03 10.34 -2.48 -13.47 -17.29 -16.44 5.89 -5.65 -21.92 -26.78 -19.25 11.02
Vacuole Periphery 1.42 -0.78 1.92 1.17 5.93 0.10 1.05 0.59 0.39 -0.30 0.49 -1.23 2.05 0.95 5.20
Standard name
Human Ortholog
Description Anthranilate synthase; catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Trp2

Trp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available